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Harvesting schedule effects on forage yield and nutritive values in low-lignin alfalfa

  • Xu, Xuan;Min, Doohong
    • Journal of Animal Science and Technology
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    • v.64 no.2
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    • pp.262-273
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    • 2022
  • Under the four-cut system, low-lignin alfalfa (Medicago sativa L.) may extend harvesting intervals improving harvest management flexibility and producing forage products with higher nutritive values. The objective of this study was to compare forage yield and nutritive values of low-lignin and conventional alfalfa varieties when applied to six different harvest schedules in the first (2016) and second (2017) production years. There were 12 treatments of two alfalfa varieties as whole plots and six harvest schedules as subplots. Across harvest schedules, there were four cuttings in two production years. Three harvest intervals including "Standard" (high quality, HQ), "Standard+5-day" (medium quality, MQ), and "Standard+10-day" (high yield, HY) were chosen for the first cutting, and 30-day (HQ) and 35-day (HY) for the second cuttings. The third and fourth cuttings in 2016 were timed near final harvest date and in 2017 occurred at 35-day (MQ) and 40-day (HY). Variety by harvest schedule interaction was not significant, but the whole plot and sub-plot effects were significant. Hi-Gest 360 was consistently higher in nutritive value and with a similar yield as Gunner. Harvest schedules did not consistently differ in forage yield and nutritive values. HS-1 ("Standard" + 35-day + Medium Quality + High Yield) with shorter first two cutting intervals provided lower acid detergent fiber (ADF), neutral detergent fiber (NDF), higher relative feed value (RFV), and similar forage yield compared to other schedules. HS-1 had the highest economic incomes when considering RFV and yield among the six different harvest schedules.

Functional Characterization of Drosophila melanogaster CYP6A8 Fatty Acid Hydroxylase

  • Sang-A Lee;Vitchan Kim;Byoungyun Choi;Hyein Lee;Young-Jin Chun;Kyoung Sang Cho;Donghak Kim
    • Biomolecules & Therapeutics
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    • v.31 no.1
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    • pp.82-88
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    • 2023
  • Genomic analysis indicated that the genome of Drosophila melanogaster contains more than 80 cytochrome P450 genes. To date, the enzymatic activity of these P450s has not been extensively studied. Here, the biochemical properties of CYP6A8 were characterized. CYP6A8 was cloned into the pCW vector, and its recombinant enzyme was expressed in Escherichia coli and purified using Ni2+-nitrilotriacetate affinity chromatography. Its expression level was approximately 130 nmol per liter of culture. Purified CYP6A8 exhibited a low-spin state in the absolute spectra of the ferric forms. Binding titration analysis indicated that lauric acid and capric acid produced type I spectral changes, with Kd values 28 ± 4 and 144 ± 20 µM, respectively. Ultra-performance liquid chromatography-mass spectrometry analysis showed that the oxidation reaction of lauric acid produced (ω-1)-hydroxylated lauric acid as a major product and ω-hydroxy-lauric acid as a minor product. Steady-state kinetic analysis of lauric acid hydroxylation yielded a kcat value of 0.038 ± 0.002 min-1 and a Km value of 10 ± 2 µM. In addition, capric acid hydroxylation of CYP6A8 yielded kinetic parameters with a kcat value of 0.135 ± 0.007 min-1 and a Km value of 21 ± 4 µM. Because of the importance of various lipids as carbon sources, the metabolic analysis of fatty acids using CYP6A8 in this study can provide an understanding of the biochemical roles of P450 enzymes in many insects, including Drosophila melanogaster.

Effect of Korean Service Quality Awards on the Market Value by using Event Study Methodology (한국의 서비스 품질상 수상이 기업가치에 미치는 영향 : 사건연구방법론적 접근)

  • Oh, Byoung-Sub;Park, Ji-Young;Chung, Soong-Hwan;Choi, Kang-Hwa
    • Korean Management Science Review
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    • v.27 no.3
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    • pp.161-196
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    • 2010
  • This paper empirically investigates the impact of winning a service quality award on the market value in Korea. We estimates the mean "abnormal" change in the stock prices of sample firms when information of winning a service quality award was publicly announced. To access the validity of the research question, this paper employed collected 47 firms data that received the Korean Service Quality Awards so far. Event study methodology was used to analyze the effect of Korean service quality awards. The findings are as follows; The average abnormal returns on the event date are not significant at the 0.05 level which means that the receiving Korean Service Quality Awards has no influence on the firms' market value. On the other hand successive awarded firms have an increasing effect on the market value and it is significant at the 0.05 level. Furthermore, the results show that the factors of firm size such as firm's total assets are critical to vary the firms' abnormal returns. There might be some limitations in this study. The most obvious problem is the limitation of sample size. Although 518 sample cases were found during the period from 2000 to 2008, most of the cases were deleted according to the sample criteria. We are expecting the future research with more data and more precise results. Furthermore, our research consider the only two service award institutions even though there are several different service award authorities in Korea. It is needed to expand the research scope and range to adopt the various service award institutions for the future work.

Relationship between the level of Sexual Contacts and Self-Esteem, Self-Assertiveness of Teenage Girls Who Have or Had Boyfriends (이성교제를 하는 십대여학생의 성접촉과 자아존중감.자기주장의 관계)

  • Lee, Sun-Kyoung
    • Women's Health Nursing
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    • v.7 no.2
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    • pp.212-228
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    • 2001
  • This is a cross-sectional descriptive correlation study that aimed to understand the relationship between the level of sexual contacts during the dates and psycho-social factors in teenage girls who have or had boyfriends. The purpose of this study was to describe the relationship between sexual contacts and self-esteem and self- assertiveness. The subjects of the study were 6,130 teenage girls who have or had boyfriends. They were selected convienently 12,733 teenage girls from 254 secondary schools located in 7 large cities and 9 provinces in Korea. A structured questionaire was used it included items one general characteristics, the levels of sexual contacts, self-esteem and self-assertiveness. Seven levels of sexual contacts were rated they were holding a hand, putting arms around each other's shoulders, kiss, french kiss, touching breast, petting genitalia, and sexual intercourse during the dates. Self-esteem and self-assertiveness were measured by the "Self-esteem scale" of Rogenberg (1971) and the questionaires developed by S. B. Chang et al(2000), respectively. The self-reported questionaires were collected from October 2, to October 28, 2000 and analyzed by using SPSS 10.0 Program. The data were analyzed by descriptive statistics, reliability, One-way ANOVA with Duncan method & Scheffe method. The results of the analysis were as follows. 1.An average Percentage of the respondents who have (46.1%) or had (53.9%) boyfriends was 48.1% (n=6,130) and the most common sexual contact during the dates was french kiss (26.7%, n=1,634). While 7.5%(458) of respondents had a sexual intercourse, 13.7%(842) of respondents didn't have any sexual contact during the dates. 2. Distribution of starting point of the first sexual contact demonstrates that 1,950 respondents (31.8%) held partner's hand at the first date and 1,367 respondents (22.3%) put arms around each other's shoulders at the 2nd or 3rd date. Sexual contacts such as kiss, french kiss, touching breast, petting genitalia, or sexual intercourse were experienced mostly at the later than the 10th date. However, surprisingly large number(1.7%) of respondents had genital contact and sexual intercourses at the first date. 3. The means of self-esteem and self-assertiveness socres of the respondents who have or had boyfriends are 27.43$({\pm}4.03)$ and 17.96$({\pm}2.55)$, respectively. The range of scores for self esteem was 10-40, 40 with most self esteem. The range of scores for self assertiveness was 7-21, 21 with most self assertiveness. 4. As sexual contacts proceeded, the means of self-esteem and self-assertiveness values decreased. In detail, the respondents having no or light sexual contacts (kiss, putting arms around each other's shoulder, holding a hand) had similar self-esteem values($p{\leq}0.05$). However, ones having relatively intensive sexual contacts (french kiss, touching breast) showed significantly lower self-esteem values($p{\leq}0.05$). Same trend has been obtained for self-assertiveness value. 5. The higher values of self-esteem and self-assertiveness values the respondents had, the later they started the moderate sexual contact (kiss, french kiss) during the dates. Interestingly, among the respondents having intensive sexual contacts (petting genitalia, sexual intercourse) during the dates, those who experienced the such contacts at the first date or later than 10th date have higher self-esteem and self-assertiveness values than one experienced the such contacts during the 2nd$\sim$9th dates, giving U-shape curve. 6. There was a significant relationship between self-esteem and self-assertiveness in girls who have or had boyfriends($P{\leq}.001$). In conclusion, the research shows that self-esteem and self-assertiveness values are significantly related with the sexual contacts of the teenage girls who have or had boyfriends. These results strongly suggest that proper sex education program for teenage girls should contain the program on improving the self-esteem and self-assertiveness. We believe that sex education program is the one of the best ways to prevent the unwanted sexual contacts and pregnancy of teenage girls.

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Brand Equity and Purchase Intention: The Fashion Market in China (상표자산이 구매의도에 미치는 영향: 중국패션시장에서)

  • Lee, Dong-Hae;Choi, Young-Ro
    • Journal of Distribution Science
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    • v.13 no.7
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    • pp.85-90
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    • 2015
  • Purpose - Global trends play a part to change the structure of the fashion industry. In particular, companies attempting to conduct innovative marketing centering on such products as SPA brands are growing into global companies. SPA stands for "Specialty Store Retailer of Private Label Apparel", meaning its activities are fully integrated from manufacturing through sales, including material procurement design, product, distribution, inventory management, and final sales. For this reason, more understanding of individual corporate profitability is very sensitive to consumer's attitudinal changes. The effects that corporate marketing activities on customer lifetime value through brand attitude were analyzed based on a structural equation model. Rust suggested value equity, brand equity, and relationship equity as customer equity driver. The study examines Chinese consumer because China is the fastest growing fashion market in the world. Research design, data, and methodology - The survey targeted Chinese college student age 20s. Only respondents who had purchased SPA brands in the past year were included for this research. A total of 303, except for 47 missing data of 350 distributed questionnaires were included in this research. The questionnaire is consists of six part to measure value, brand, relationship equity, attitude toward brand, purchase intention and demographic characteristics. This research conducted exploratory factor analysis and reliability test. To verify research hypotheses, structural equation model test was conducted. As for customer equity, diversified models in consideration of the scope of acquisition data, a method of collection of data, influencing factor, and predictability were suggested based on a net present value model. However, the history of customer equity study is relatively short, and sufficient empirical analyses have not been conducted, so more integrated analysis is required. In this study, the concept of driver suggested by Rust was applied to figure out the effects that consumer's attitude has on customer equity. The customer equity driver suggested by them consists of brand equity, value equity, and relationship equity. Results - This study reveals that value equity and brand equity have a positive influence on relationship equity. And, relationship equity has a positive influence on purchase intention through brand attitude. However, value equity and brand equity do not influence on brand attitude. Conclusion - The results of this research generated following implications. First, SPA brands need to take advantage of their value equity such as perceived low price and up-to-date fashion style to attract Chinese young consumer. Second, strong brand equity promises dominants position in the competitive market. As Chinese fashion market grows rapidly, SPA brands can consider branding strategy such as flagship store and celebrity marketing enhancing brand image. Third, the core concept of customer equity strategy is to maintain a relationship with their expecting and existing customers. The relationship equity is built by brand equity and value equity. When SPA brands serves product and service meet with individual customers, customers have intimacy to the brands.

A Study on the Software development for the correlation of the water pollution of pollution material analysis (공해물질분석에 관한 수질오탁의 상관성 계산 위한 SOFTWARE 개발에 관한 연구)

  • 택훈
    • Journal of the Speleological Society of Korea
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    • v.15 no.16
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    • pp.38-60
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    • 1987
  • There are BOD(Biochemical Oxygen Demand) and COD(Chemical Oxygen Demand) in the date of the water pollution in the pollution analysis, BOD and COD analysis the result of the analysis. The computer developed the program in the analysis. Went by BOD, the relation conversion equation type of COD, the control chart, the standard deviation, and the coefficient of correlation etc.. for the purpose of the development of the program. There is a purpose by which it is what coefficient of correlation in the development of this program or not if the value (X and Y) of unknow is understood. In the future, if this program is applied the utilization to not only the data is analyzed about the water pollution but also a lot of fields is proposed.

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Evaluation of Growth Characteristics and Forage Yield of Domestically Bred Silage Corn Varieties

  • Kim, Jong Geun;Yu, Young Sang;Wang, Li Li;Li, Yan Fen
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.3
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    • pp.146-154
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    • 2022
  • This experiment was conducted to evaluate the growth characteristics and productivity of silage corn varieties developed in Korea. Corn cultivation was carried out using the experimental field in the Pyeongchang campus of Seoul National University (550 m above sea level). There have 10 domestic cultivars (Gwangpyeongok, Dacheongok, Yanganok, Jangdaok, Cheongdaok, Daanok, Sinhwangok, Sinhwangok II, Pyeonggangok, and Hwangdaok) with one imported cultivar (P1543) which tested as a control, and randomized block design with three replications. Among the 100-grains weight of the seeds, Dacheongok was the heaviest, and the germination rate for each variety was 74.6% on average, while that of Daanok and Sinhwangok were over 90%. Sinhwangok was the fastest in tasseling and silking date. The number of days required to be silking date was as slow as 85 days in Dacheongok, Cheongdaok and Pyeonggangok, and as fast as 80 days or less in Sinhwangok, Sinhwangok II and Hwangdaok. The plant height of P1543 was the highest as 344cm, and Hwangdaok and Daanok were short. In terms of the ratio of ears, Daanok had the highest rate of 60.18%, and Jangdaok and Dacheongok had the lowest. There was no significant difference in dry matter content in stover, but P1543 was generally higher in ear and total dry matter content. The dry matter yield was highest in P1543, and the yield of TDN was significantly higher in P1543 and Yanganok. There was a significant difference in the crude protein content of ears and the dry digestibility of stems (p<0.05), while there was no significant difference in the content of each part or element. Combining the above results, Yanganok was the highest in terms of yield, and Dacheongok, Sinhwangok and Pyeonggangok were also recommended for domestically grown corn varieties in the mountainous regions of Gangwon-do.

Vegetation Changes and Yields of Tall Fescue-based Mixture Pasture in the Central Region of South Korea

  • Jeong Sung Jung;Se Young Lee;Mirae Oh;Hyung Soo Park;Bae Hun Lee;Ki Choon Choi
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.4
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    • pp.199-205
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    • 2023
  • Pasture formation and management are crucial to avoid yield reduction. This experiment aimed to examine the effects of tall fescue-centered mixed-seeding combinations on yield and vegetation changes in perennial pastures in the central region for two years, from September 2020 to October 2022. The treatments were arranged in three replications in a randomized block design: control (C), tall fescue-based mixture-1 (T-1), and tall fescue-based mixture-2 (T-2). The tall fescue (TF), orchard grass (OG), perennial ryegrass (PRG), Kentucky bluegrass (KBG), and white clover (WC) were used. The emergency rate of grasses (70.0 to 73.3%) did not differ among mixed seeding combinations. Overwintering rates (81.7 to 83.3%) were similar among treatments. The plant height of grasses was similar at each harvest date, with the highest height (86.2 cm) recorded in the second harvest of the first year, followed by that (58.4 cm) in the third harvest of the first year; it was least (38.9 cm) in the fourth harvest of the second year. There was no significant difference in the dry matter yield of grasses among the mixed seeding combination treatments in the first, third, or fourth harvests of the first year (p>0.05). For second-year grasses, dry matter yield was not significantly different in harvest date among the treatments (p>0.05). Based on mixed seeding ratio, orchard grass showed the highest yield at 70% in the C treatment, followed by tall fescue at 80% and 60% in the T-1 and T-2 treatments, respectively, in the first harvest after seeding. There was no significant difference in feed value between treatments (p>0.05), but a significant difference was observed between the third and fourth harvest (p<0.05). Therefore, it indicated that it is important to create perennial pastures in the central region through mixed seeding combinations centered on tall fescue.

Effect of Operational Parameters on the Products from Catalytic Pyrolysis of Date Seeds, Wheat Straw, and Corn Cob in Fixed Bed Reactor

  • Sultan Mahmood;Hafiz Miqdad Masood;Waqar Ali khan;Khurram Shahzad
    • Korean Chemical Engineering Research
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    • v.61 no.4
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    • pp.591-597
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    • 2023
  • Pakistan depends heavily on imports for its fuel requirements. In this experiment, catalytic pyrolysis of a blend of feedstock's consisting of date seed, wheat straw, and corn cob was conducted in a fixed bed reactor to produce oil that can be used as an alternative fuel. The main focus was to emphasize the outcome of important variables on the produced oil. The effects of operating conditions on the yield of bio-oil were studied by changing temperature (350-500 ℃), heating rate (10, 15, 20 ℃/min), and particle size (1, 2, 3 mm). Moreover, ZnO was used as a catalyst in the process. First, the thermal degradation of the feedstock was investigated by TGA and DTG analysis at 10 ℃/min of different particle sizes of 1, 2, and 3mm from a temperature range of 0 to 1000 ℃. The optimum temperature was found to be 450 ℃ for maximum degradation, and the oil yield was indicated to be around 37%. It was deduced from the experiment that the maximum production of bio-oil was 32.21% at a temperature of 450 ℃, a particle size of 1mm, and a heating rate of 15 ℃/min. When using the catalyst under the same operating conditions, the bio-oil production increased to 41.05%. The heating value of the produced oil was 22 MJ/kg compared to low-quality biodiesel oil, which could be used as a fuel.

Probing Tidal Field Strength of Virgo Cluster Galaxies

  • Yoon, Hye-In;Chung, Ae-Ree
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.2
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    • pp.74.2-74.2
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    • 2011
  • We probe the tidal perturbation parameter f of Virgo cluster galaxies. The goal is to measure the strength of tidal fields around individual galaxies to get better understanding gravitational processes that can affect galaxy evolution in the cluster environment. The f-value is defined as a logarithmic ratio between the net internal gravitational force within a galaxy and the external tidal force exerted by a neighboring galaxy. Hence, it provides one way to quantify the tidal field strength of galaxies, in particular, due to galaxy neighbors. In this study, we determine f-values of the VIVA galaxies, samples of the VLA Imaging study of Virgo galaxies in Atomic gas, using the Extended Virgo Cluster Catalog (EVCC) which is the most complete Virgo cluster catalog to date. With diagnostics based on the HI gas and R-band morphologies, we discuss the impact of the tidal fields on the evolution of the VIVA sample. Also, we compare the tidal field strength to the intra cluster medium (ICM) pressure for each sample galaxy to pin down environmental processes at work.

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