• 한국식생활문화학회지
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• 제10권5호
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• pp.443-448
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• 1995

Korean fresh elephant-foot (Amorphophalus konjak K. Koch) and its powder were analyzed and compared with foreign samples to investigate the physico-chemical characteristics of Korean konjak. The Korean fresh konjak contained 80.6% of moisture content and most of the solid component comprises much of sugar, protein and trace of fat and fibre. The mannan content of Korean konjak powder is far smaller than those of Japanese and Chinese konjak powder. The analysis of the Korean konjak revealed that glutamic acid, aspartic acid and arginine included 45% of total amount, and other amino acid was incresaed with the order of valine, serine, leucine and glycine. The Korean konjak contained a moderate amount of K component and other inorganic component was larger with the order of P, Na and Ca. The yield of refined powder obtained from dried chip of Konjak was 61.0% in Korean one and 57.5% in Chinese one. The degree of lightness of Chinese konjak powder was slightly higher than that of Korean product, but the difference could not be recognized by naked eye.

• 생약학회지
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• 제21권3호
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• pp.235-238
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• 1990

Fatty acids, sterols and amino acids were confirmed from seeds of Acanthopanax senticosus for. inermis Harms. Fatty acids were isolated from petroleum ether extract and identified by gas liquid chromatography. Its composition was myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid and behenic acid. The composition of sterols that isolated from n-hexane extract were determined by GC/MS. It was confirmed to be a mixture of ${\beta}-sitosterol$ and stigmasterol. And several kinds of amino acids-cysteic acid, threonine, serine, proline, glutamic acid, glycine, alanine, valine, isoleucine, leucine, histidine, lysine and arginine-were confirmed from water fraction.

• 한국식품조리과학회지
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• 제12권3호
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• pp.273-278
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• 1996

To investigate the main components of Korean traditional soy sauce for its typical taste, we compare the physicochemical properties of Korean traditional soy sauce and commercial soy sauce. The physicochemical analysis revealed that each components showed significant difference between Korean traditional soy sauce and commercial soy sauce. The significant characters for discrimination between Korean traditional soy sauce and commercial soy sauce were salt content, ammonia nitrogen content and total acidity (R$^2$=0.99). The components of Korean traditional soy sauce divided into 3 clusters, and each group is characterized as formol nitrogen, salt and total nitrogen content by cluster analysis. Main amino acids of Korean traditional soy sauce were alanine, glutamic acid, leucine and valine.

• BMB Reports
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• 제31권1호
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• pp.37-43
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• 1998

The catabolic ${\alpha}$-acetolactate synthase was purified to homogeneity from Serratia marcescens ATCC 25419 using ammonium sulfate fractionation, DEAE-Sepharose, Phenyl-Sepharose, and Hydroxylapatite column chromatography. The native molecular weight of the enzyme was approximately 150 kDa and composed of two identical subunits with molecular weights of 64 kDa each. The N-terminal amino acid sequence of the enzyme was determined to be Ala-Gln-Glu-Lys-Thr-Gly-Asn-Asp-Trp-Gln-His-Gly-Ala-Asp-Leu-Val-Val-Lys-Asn-Leu. It was not inhibited by the branched chain amino acids and sulfometuron methyl herbicide. The optimum pH of the enzyme was around pH 5.5 and the pI value was 6.1. The catabolic ${\alpha}$-acetolactate synthase showed weak immunological relationships with recombinant tobacco ALS, barley ALS, and the valine-sensitive ALS isozyme from Serratia marcescens.

• Asian-Australasian Journal of Animal Sciences
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• 제12권6호
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• pp.956-965
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• 1999

Genetic selection and sophisticated management technology have produced modern sows which excel in litter size and milk production. Recent research has identified nutritional innovations which may enhance productivity of the high producing sow. Selected research in three areas which have the potential to enhance reproductive performance are summarized in this report. First, preliminary evidence indicates that organic chromium may enhance litter size and conception rate. Secondly, high producing sows, particularly primiparous sows, required higher protein/lysine in late gestation and or lactation to optimize weaning weight and subsequent litter size. Valine and isoleucine, but not leucine, have been shown to enhance milk production. Phased feeding programs with diet cost partitioned toward gilts and away form parity 3 to 8 sows have been suggested as a means of improving reproductive performance in young sows. Lastly, recent research with sufficient sow numbers to document the effect of dietary fiber on reproductive performance has shown that sows fed fiber farrowed and weaned more pigs.

• 한국자기공명학회논문지
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• 제4권2호
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• pp.133-139
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• 2000

The strategy to incorporate [$^{15}$ N]-labeled amino acids were discussed. Instead of using specific auxotrophic strains for selective labeling, the prototrophic strain, BL2l(DE3), was used with a plasmid, pLysS, and found to be very effective for several amino acids including alanine, lysine, leucine, and threonine. Isoleucine, valine, glutamine, and tyrosine were also found to be effective despite some diffusion into other amino acids. Interesting result was obtained when [$^{15}$ N]-labeled glycine was tried: only glycines were labeled when amino acid mixture was added in the growth medium, and serines were co-labeled when amino acids were omitted. These results can be used as a guideline when selective labeling strategy is considered, and when the resulting data are interpreted.

• Journal of Nutrition and Health
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• 제9권2호
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• pp.98-101
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• 1976

The essential amino acids composition of Green algae (chlorella ellipsoidea) that was isolated near the suwon was compared with that of soy flour and the provisional pattern of amino acids of reference protein of FAO and found that the limit amino acid was methionine. The rest of essential amino acids were distributed widely and abunduntly. While soy flour was defficient in methionine and valine on comparing with the provisional pattern. Slightly more content of methionine was recognized in chlorella protein than that of spirulina maxima which was reported to be eaten in the Lake Chard region of Africa and Mexico as a daily food. It was concluded that good quality protein is able to be synthesised from the wild alage if a qualified alage media was given to them.

• 대한의생명과학회지
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• 제7권3호
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• pp.127-131
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• 2001

This study was undertaken to examine the effect of amino acids on anoxia-induced cell injury in rabbit renal cortical slices. In order to induce anoxic cell injury, slices were exposed to a 100% $N_2$ atmosphere and control slices were exposed to 100% $O^2$. Irreversible cell injury was estimated by measuring lactate dehydrogenase (LDH) release and alterations in renal cell function were examined by measuring p-aminohippurate (PAH) uptake. Anoxia caused the increase in LDH release in a time-dependent manner. Glycine and glutathione almost completely prevented anoxia-induced LDH release. Of amino acids tested, glycine and alanine exerted the protective effect against anoxia-induced cell injury. However, asparagine with amide side chain, leucine and valine with hydrocarbon side chain, and basic amino acids (lysine, histidine, and arginine) were not effective. Anoxia-induced inhibition of PAM uptake was prevented by glycine. ATP content was decreased by anoxia, which was not affected by glycine. Anoxia-induced depletion of glutathione was significantly prevented by glycine. These results suggest that neutral amino acids with simple structure exert the Protective effect against anoxia-induced cell injury the involvement of specific interaction of amino acids and cell structure.

• 미생물과산업
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• 제16권1호
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• pp.7-17
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• 1990

In summary, the nature of the amino acid changes that impart oncogenicity in either the focus formation or tumorigenicity assay can be inferred by analysis of PCR-amplified DNA from tumor-derived NIH3T3 transformants and confirmed by analysis of primary liver tumors. Putative activating mutations in the c-K-ras genetic locus have been shown to involve a single-base modification of either G-C base pair at codon 12 leading to aspartate or cystein substitutions for glycine. The oncogenicity of an N-ras oncogene containing the N-ras C gene region may be related to an amino acid substitution of valine for glycine at codon 13.

• 한국응용과학기술학회지
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• 제3권1호
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• pp.33-37
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• 1986

The composition of fatty acid and amino acid for Seong-ge-al(sea urchin) were analysed by the automatic analyser. There are two kind of samples such as raw and saited, fermented one. The results obtained are as follows. 1. In the general composition, crude protein, crude lipid and ash as 15.8%, 3.5% and 2.2%, respectively. The raw sample showed pH 5.6 value. 2. In case of amino acid, it contained 17 different kind of amino acids. Among them, a lot of glutamic acid, aspartic acid, arginine, glycine and valine were contained. 3. In case of fermentation period, threonine, histidine, lysine and arginine were increased a little bit. 4. In case of fatty acid, it contained 13 different kind of fatty acids. Among them, a lot of palmitic acid, behnic acid, linoleic acid and arachidonic acid were contained.