• 한국작물학회지
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• 제55권4호
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• pp.357-364
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• 2010

Kjeldahl method used in many materials from various plant parts to determine protein contents, is laborious and time-consuming and utilizes hazardous chemicals. Near-infrared (NIR) reflectance spectroscopy, a rapid and environmentally benign technique, was investigated as a potential method for the prediction of protein content. Near-infrared reflectance spectra(1100-2400 nm) of coarse cereal grains(n=100 for each germplasm) were obtained using a dispersive spectrometer as both of grain itself and flour ground, and total protein contents determined according to Kjeldahl method. Using multivariate analysis, a modified partial least-squares model was developed for prediction of protein contents. The model had a multiple coefficient of determination of 0.99, 0.99, 0.99, 0.96 and 0.99 for foxtail millet, sorghum, millet, adzuki bean and mung bean germplasm, respectively. The model was tested with independent validation samples (n=10 for each germplasm). All samples were predicted with the coefficient of determination of 0.99, 0.99, 0.99, 0.91 and 0.99 for foxtail millet, sorghum, millet, adzuki bean and mung bean germplasm, respectively. The results indicate that NIR reflectance spectroscopy is an accurate and efficient tool for determining protein content of diverse coarse cereal germplasm for nutrition labeling of nutritional value. On the other hands appropriate condition of cereal material to predict protein using NIR was flour condition of grains.

• 한국항공우주학회지
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• 제40권2호
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• pp.146-156
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• 2012

비행 중 결빙은 항공기 운용에 위험한 상황을 초래하게 되며, 개발 및 감항 인증 과정중 성능과 안전에 미치는 영향이 평가되어야 한다. 평가를 위한 결빙 풍동시험에서 스케일링 기법은 실제와 동등한 수집율과 결빙량이 모사되는 대체시험 조건을 결정하는 기법이다. 본 논문에서는 Olsen 및 Ruff-IV 기법과 무차원 Weber 수를 일치시켜 속도를 계산하는 방법을 적용하여 연구자들이 쉽게 이용할 수 있도록 스케일링 프로그램을 개발하였다. 동일조건에서 수행된 NASA 스케일링 결과와 비교하여 프로그램을 검증하였다. 또한 FAR Part 25 Appendix C를 적용한 스케일링 사례를 제시하고 결빙코드 FENSAP-ICE를 이용하여 스케일링 기법을 검증하였다.

• 대한지역사회영양학회지
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• 제25권4호
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• pp.317-328
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• 2020

Objectives: The objective of this study was to verify the validity of a mobile phone application (app) that applies a 24-hour dietary recall with meal photos, as a means of being a more accurate method of estimating dietary sodium intake. Methods: Of the 203 subjects enrolled, 172 subjects (84 males and 88 females) were selected for the final analysis, excluding those with an intake less than 500 kcal and urine output less than 500 ml. Dietary sodium assessment methods used for comparing with the 24-hour urinary sodium excretion are as follows: 1) face-to-face 24-hour dietary recall, 2) 24-hour dietary recall using the mobile app, 3) face-to-face 24-hour dietary recall considering liquid intakes from soup, stew, water kimchi and noodle, etc (liquid-based dishes), 4) 24-hour dietary recall using the mobile app considering liquid intakes from liquid-based dishes, and 5) food frequency questionnaire. Repeated ANOVA with Bonferroni method was used for comparing the average sodium intake, and Pearson's correlation was applied to correlate the methods used. Results: In women, no significant difference was observed in the average sodium intake between all methods. Moreover, analysis in men and total adults revealed no significant difference between the 24-hour urinary sodium secretion, and 24-hour dietary recall using the app and 24-hour dietary recall using the app considering liquid intakes. Sodium intake by food frequency questionnaire was significantly different when compared with the intake determined from 24-hour urinary sodium excretion. Sodium intake from all methods (except food frequency questionnaire) significantly correlated with values obtained from 24-hour urine sodium excretion. Conclusions: Results of this study validated a mobile phone app using a 24-hour dietary recall with meal photos to better estimate dietary sodium intakes. It is believed that further studies in the future will enable the application as a tool to more accurately determine sodium intake.

• 한국산학기술학회논문지
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• 제13권5호
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• pp.1976-1982
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• 2012

압축기는 다양한 유체기계에 널리 사용되고 있으며, 특히 압축기의 성능은 냉동공조 시스템의 성능계수에 직접적인 영향을 미치므로 압축기의 효율을 높이기 위한 노력이 계속되어 왔다. 용도에 맞게 다양한 종류의 압축기가 개발되었고, 그 중 스크롤 압축기는 그 크기에 비하여 압축 성능이 우수하여 여러 분야에서 선호하는 압축기이다. 스크롤 압축기의 개발을 위하여 실험적인 연구는 많이 수행되어 온 반면, 개발 목적에 맞게 압축기를 해석할 수 있는 모델은 많지 않은 실정이다. 따라서 본 연구에서는 스크롤 압축기를 기하학적으로 모델링하여 해석할 수 있는 모델을 1-D 상용 프로그램인 AMESim을 이용하여 개발하였으며, 실험 데이터를 이용하여 모델의 신뢰성을 검증하였다. 본 연구 결과는 추후 스크롤 압축기를 개발하는 과정에서 압축기의 성능을 예상하고 최적화 할 수 있는 방법을 제공할 것으로 기대된다.

• 대한공간정보학회지
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• 제19권3호
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• pp.83-90
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• 2011

본 논문의 목적은 공업용수 사용 추세패턴 모형을 개발하고 개발된 모형이 GIS시스템내에서 활용될 수 있는 청사진을 제시하는데 있다. 연구내용은, 사용데이터의 수집을 위해 실시간 모니터링 테크닉이 도입되었고 실시간 데이터는 5분단위로 센서 및 현장서버로부터 관리서버로 전송되었다. 취득된 데이터는 선택된 다항식에 대입되었고 결과로 요일별, 각 월의 일평균 수요모형들이 개발되었다. 도출된 모형들은 일련의 검증과정을 거쳐 최종 모형으로 압축선택되며 평균모형으로 변환되었다. 변환된 평균모형의 도식화를 통해 공업용수 수요패턴분석이 이루어졌다. 연구결과로, 수요패턴은 상당한 일관성을 보이고 있어 확률높은 요일별, 또는 계절별 수요예측이 가능하다는 결론이 도출되었다. 또한 이러한 예측모형을 활용할 정보화도구로서 GIS의 활용방안이 제시된다.

• 한국항공우주학회지
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• 제48권8호
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• pp.631-640
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• 2020

현재 항공기 시뮬레이터는 실제 항공기를 이용한 조종사 훈련에 비해 경제적, 시간적 및 공간적 비용 측면에서 상당한 이점을 가지고 있어서 많은 관심을 받고 있다. 항공기 시뮬레이터의 구성 요소 중 비행역학 모델은 실제 항공기의 비행을 모의하는데 중요한 역할을 한다. 따라서 자동화된 도구를 사용하여 비행역학 모델의 충실도를 검증하는 것은 중요한 의미를 지닌다. 본 논문에서는 항공기 시뮬레이터의 효율적인 개발을 위하여 비행역학 모델의 충실도를 자동 검증하는 소프트웨어를 개발한다. 충실도 검증 프로세스로부터 도출된 요구사항에 대하여 소프트웨어 구조 및 GUI를 설계한 다음 Window 기반 환경에서 C# 언어를 이용하여 소프트웨어를 구현한다. CTSW 기종에 대한 실험 결과는 개발된 소프트웨어가 기능 및 성능, 그리고 사용자 편의성 측면에서 효과적임을 보여준다.

• 한국축산시설환경학회지
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• 제20권3호
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• pp.117-124
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• 2014

The objectives were to assess the use of near-infrared reflectance spectroscopy (NIRS) as a tool for estimating nutrient compositions of corn kernel, and to apply an NIRS-based indium gallium arsenide array detector to the system for collecting spectra and analyzing calibration equations using equipments designed for field application. Partial Least Squares Regression (PLSR) was employed to develop calibration equations based on representative data sets. The kennard-stone algorithm was applied to induce a calibration set and a validation set. As a result, the method for structuring a calibration set significantly affected prediction accuracy. The prediction of chemical composition of corn kernel resulted in the following (kennard-stone algorithm: relative) moisture ($R^2=0.82$, RMSEP=0.183), crude protein ($R^2=0.80$, RMSEP=0.142), crude fat ($R^2=0.84$, RMSEP=0.098), crude fiber ($R^2=0.74$, RMSEP=0.098), and crude ash ($R^2=0.81$, RMSEP=0.048). Result of this experiment showed the potential of NIRS to predict the chemical composition of corn kernel.

• 대기
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• 제26권2호
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• pp.277-288
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• 2016

Polar lows are intense mesoscale cyclones that mainly occur over the sea in polar regions. Owing to their small spatial scale of a diameter less than 1000 km, simulating polar lows is a challenging task. At King Sejong station in West Antartica, polar lows are often observed. Despite the recent significant climatic changes observed over West Antarctica, adequate validation of regional simulations of extreme weather events such as polar lows are rare for this region. To address this gap, simulation results from a recent version of the Polar Weather Research and Forecasting model (Polar WRF) covering Antartic Peninsula at a high horizontal resolution of 3 km are validated against near-surface meteorological observations. We selected a case of high wind speed event on 7 January 2013 recorded at Automatic Meteorological Observation Station (AMOS) in King Sejong station, Antarctica. It is revealed by in situ observations, numerical weather prediction, and reanalysis fields that the synoptic and mesoscale environment of the strong wind event was due to the passage of a strong mesoscale polar low of center pressure 950 hPa. Verifying model results from 3 km grid resolution simulation against AMOS observation showed that high skill in simulating wind speed and surface pressure with a bias of $-1.1m\;s^{-1}$ and -1.2 hPa, respectively. Our evaluation suggests that the Polar WRF can be used as a useful dynamic downscaling tool for the simulation of Antartic weather systems and the near-surface meteorological instruments installed in King Sejong station can provide invaluable data for polar low studies over West Antartica.

• The Korean Journal of Physiology and Pharmacology
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• 제13권5호
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• pp.373-378
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• 2009

Cerebellar Purkinje cells (PCs) play a crucial role in motor functions and their progressive degeneration is closely associated with spinocerebellar ataxias. Although immunohistochemical (IHC) analysis can provide a valuable tool for understanding the pathophysiology of PC disorders, the method validation of IHC analysis with cerebellar tissue specimens is unclear. Here we present an optimized and validated IHC method using antibodies to calbindin D28k, a specific PC marker in the cerebellum. To achieve the desired sensitivity, specificity, and reproducibility, we modified IHC analysis procedures for cerebellar tissues. We found that the sensitivity of staining varies depending on the commercial source of primary antibody. In addition, we showed that a biotin-free signal amplification method using a horseradish peroxidase polymer-conjugated secondary antibody increases both the sensitivity and specificity of ICH analysis. Furthermore, we demonstrated that dye filtration using a $0.22\;{\mu}m$ filter eliminates or minimizes nonspecific staining while preserving the analytical sensitivity. These results suggest that our protocol can be adapted for future investigations aiming to understand the pathophysiology of cerebellar PC disorders and to evaluate the efficacy of therapeutic strategies for treating' these diseases.

• Journal of Microbiology and Biotechnology
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• 제20권10호
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• pp.1463-1470
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• 2010

E. coli has long been widely used as a host system for the manufacture of recombinant proteins intended for human therapeutic use. When considering the impurities to be eliminated during the downstream process, residual host cell DNA is a major safety concern. The presence of residual E. coli host cell DNA in the final products is typically determined using a conventional slot blot hybridization assay or total DNA Threshold assay. However, both the former and latter methods are time consuming, expensive, and relatively insensitive. This study thus attempted to develop a more sensitive real-time PCR assay for the specific detection of residual E. coli DNA. This novel method was then compared with the slot blot hybridization assay and total DNA Threshold assay in order to determine its effectiveness and overall capabilities. The novel approach involved the selection of a specific primer pair for amplification of the E. coli 16S rRNA gene in an effort to improve sensitivity, whereas the E. coli host cell DNA quantification took place through the use of SYBR Green I. The detection limit of the real-time PCR assay, under these optimized conditions, was calculated to be 0.042 pg genomic DNA, which was much higher than those of both the slot blot hybridization assay and total DNA Threshold assay, where the detection limits were 2.42 and 3.73 pg genomic DNA, respectively. Hence, the real-time PCR assay can be said to be more reproducible, more accurate, and more precise than either the slot blot hybridization assay or total DNA Threshold assay. The real-time PCR assay may thus be a promising new tool for the quantitative detection and clearance validation of residual E. coli host cell DNA during the manufacturingprocess for recombinant therapeutics.