• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 추계학술대회논문집B
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• pp.247-254
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• 2000

The base pressure of vacuum vessel of the KSTAR (Korea Superconducting Tokamak Advanced Research) Tokamak is to be a ultra high vacuum, $10^{-6}{\sim}10^{-7}Pa$, to produce clean plasma with low impurity containments. For this purpose, the KSTAR vacuum vessel and plasma facing components need to be baked up to at least $250^{\circ}C,\;350^{\circ}C$ respectively, within 24 hours by hot nitrogen gas from a separate baking/cooling line system to remove impurities from the plasma-material interaction surfaces before plasma operation. Here by applying the implicit numerical method to the heat balance equations of the system, overall temperature distributions of the KSTAR vacuum vessel and plasma facing components are obtained during the whole baking process. The model for 2-dimensional baking analysis are segmented into 9 imaginary sectors corresponding to each plasma facing component and has up-down symmetry. Under the resulting combined loads including dead weight, baking gas pressure, vacuum pressure and thermal loads, thermal stresses in the vacuum vessel during bakeout are calculated by using the ANSYS code. It is found that the vacuum vessel and its supports are structurally rigid based on the thermal stress analyses.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2021년도 학술발표회
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• pp.117-117
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• 2021

본 연구에서는 Sentinel-1A/B C-band SAR(Synthetic Aperture Radar) 위성영상을 기반으로 인공신경망(Artificial Neural Network, ANN) 모형을 활용해 금강 유역 상류 40×50 km² 면적에 대한 토양수분을 산정하였다. 10 m 공간 해상도의 Sentinel-1A/B SAR 영상은 8일 간격으로 2015년부터 2019년까지 5년 동안 구축하였고, SNAP(SentiNel Application Platform)을 통해 기하 보정, 방사 보정 및 잡음(Noise) 보정을 수행하고 VV 및 VH 편파 후방산란계수로 변환하였다. ANN 모형 검증자료로 TDR(Time Domain Reflectometry)로 측정된 9개 지점의 실측 토양수분 자료를 구축하였으며, 수문학적 개념인 선행강우를 고려하기 위해 동지점에 대한 강수량 자료를 구축하였다. ANN은 각 지점에 해당하는 토양 속성별로 모델링하고, 전체 기간 및 계절별로 나누어 모의하였으며, 전체 자료의 60%와 40%를 각각 훈련 및 테스트 데이터로 사용하였다. 산정된 토양수분은 상관계수(Correlation Coefficient, R)와 평균제곱근오차(Root Mean Square Error, RMSE)를 활용하여 검증을 수행할 예정이다.

• 대한미생물학회지
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• 제19권1호
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• pp.73-77
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• 1984

Vibrio vulnificus is an organism capable of causing septicemia and wound infection in compromised patients. The source of infection is known to be raw oysters and others. The prevalence of the organism in Korean sea water and shellfish is not known. The authors surveyed shellfish and other specimens obtained mainly from a market in Seoul and from ones in Inchon. Five cultures of V. vulnificus were isolated from oyster and clam samples. Two isolates had typical characteristics of the strains isolated from patients, i.e., definite hemolysis and typical biochemical reactions. However, other 2 isolates were sucrose positive, although the identity were confirmed by Center for Disease Control. We do not know wether such strains are pathogenic or not. For the isolation of V. vulnificus from environmental samples, TCBS agar and VV agar were not very selective or differential. We isolated our strains with the use of OF-lactose agar and SPS agar. However OF-lactose agar did not support good growth of V. vulnificus, while SPS agar did not suppress other vibrios.

• 대한원격탐사학회지
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• 제10권2호
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• pp.49-62
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• 1994

Look direction bias in a single look SAR image can often be misinterpreted in the geological application of radar data. This paper investigates digital processing techniques for SAR image data integration and compensation of the SAR data look direction bias. The two important approaches for reducing look direction bias and integration of multiple SAR data sets are (1) principal component analysis (PCA), and (2) wavelet transform(WT) integration techniques. These two methods were investigated and tested with the ERS-1 (VV-polarization) and CCRS*s airborne (HH-polarization) C-SAR image data sets recorded over the Sudbury test site, Canada. The PCA technique has been very effective for integration of more than two layers of digital image data. When there only two sets of SAR data are available, the PCA thchnique requires at least one more set of auxiliary data for proper rendition of the fine surface features. The WT processing approach of SAR data integration utilizes the property which decomposes images into approximated image ( low frequencies) characterizing the spatially large and relatively distinct structures, and detailed image (high frequencies) in which the information on detailed fine structures are preserved. The test results with the ERS-1and CCRS*s C-SAR data indicate that the new WT approach is more efficient and robust in enhancibng the fine details of the multiple SAR images than the PCA approach.

• BMB Reports
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• 제42권1호
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• pp.47-52
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• 2009

Alanine racemase catalyzes the interconversion of L-alanine and D-alanine and plays a crucial role in spore germination and cell wall biosynthesis. In this study, alanine racemase produced by Bacillus anthracis was expressed and purified as a monomer in Escherichia coli and the importance of lysine 41 in the cofactor binding octapeptide and tyrosine 270 in catalysis was evaluated. The native enzyme exhibited an apparent $K_m$ of 3 mM for L-alanine, and a $V_{max}$ of $295\;{\mu}moles/min/mg$, with the optimum activity occurring at $37^{\circ}C$ and a pH of 8-9. The activity observed in the absence of exogenous pyridoxal 5'-phosphate suggested that the cofactor is bound to the enzyme. Additionally, the UV-visible absorption spectra indicated that the activity was pH independece, of VV-visible absorption spectra suggests that the bound PLP exists as a protonated Schiff's base. Furthermore, the loss of activity observed in the apoenzyme suggested that bound PLP is required for catalysis. Finally, the enzyme followed non-competitive and mixed inhibition kinetics for hydroxylamine and propionate with a $K_i$of $160\;{\mu}M$ and 30 mM, respectively.

• 한국시뮬레이션학회논문지
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• 제18권3호
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• pp.113-122
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• 2009

사용자가 요구하는 기능과 특성에 대한 다양한 요구사항은 소프트웨어를 점점 더 복잡하게 만들고 이를 검증하고 검사하는 것은 어려운 문제이다. 게다가 개발된 소프트웨어 코드를 검증하고 검사를 수행하는 과정에서 트레이싱 혹은 로깅 코드를 삽입하는 과정과 검사가 완료되어 삭제하는 과정에서 사용자의 부주의로 버그가 삽일될 수 있는 문제점도 발생한다. 본 논문은 소프트웨어 개발 과정 중에서 검증 및 검사 과정을 지원할 수 있는 Aspect DEVS 검증 틀을 제시한다. Aspect DEVS 검증틀은 관점 지향 프로그래밍 기법을 사용하여 이미 구현되어 있는 소프트웨어와 사용자는 요구사항을 만족하는 지를 확인하는 동시에 소스 코드에 테스팅 코드를 삽입할 때 발생하는 문제들을 해결한다. Aspect DEVS 검증틀을 사용한 검증의 첫 번째 단계는 관찰 대상을 관점 지향 프로그래밍 기법을 사용하여 명세한 하고, 사용자의 요구사항을 DEVS 다이어그램을 명세한 후 이를 DEVSIM++로 구현한다. 마지막으로 프로그램의 수행 과정 중에서 발생하는 이벤트들을 대상으로 구현한 DEVS 모델의 이벤트 입력으로 넣어 소프트웨어가 사용자의 요구사항을 만족하는 지를 검사함으로써 검증 과정 중에 대상 소프트웨어의 수정 없이 검증 및 검사를 수행할 수 있다.

• 대한화학회지
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• 제28권1호
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• pp.26-33
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• 1984

HF와 DF 혼합계내에서 일어나는 다음 두 진동-진동 에너지 이동 반응의 속도상수$(k_{vv})$를 n=2~5에 대해 300~800K 온도 범위에서 이론적으로 계산하였다. HF(v=n) + DF(v=0) ${\to}$ HF(v=n-l) + DF(v=l) + ${\Delta}E$(a) DF(v=n) + HF(v=0) ${\to}$ DF(v=n-l) + HF(v=l) + ${\Delta}E$(b) 이용한 충돌모형은 수소결합에너지를 분자간 상호작용의 주축으로 삼는 loosely-held, non-rigid dimer model이었고, 계산법으로는 반고전적 방법을 사용하였다. 계산 결과 반응(a)에 대한 속도상수가 반응(b)에 대한 속도상수 보다 훨씬 HF-DF 혼합계 내에서는 진동에너지가 HF에서 DF로 이동함이, 또한 반응(a)에 대한 속도 상수는 온도가 높아질 수록 감소하고, 진동 양자수가 커질 수록 증가하는 반면에, 반응 (b)에 대한 속도 상수는 온도 의존성과 양자수 의존성에 있어서 대체로 이와 반대의 경향을 보임을 밝혔으며, 이 계산 결과는 주로 에너지 차 ${\Delta}E$의 부호와 크기로써 설명될 수 있음을 보였다.

• 전자공학회논문지C
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• 제35C권11호
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• pp.39-47
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• 1998

본 논문에서는 공유 버퍼 ATM 스위치를 위한 파이프라인 방식의 고속 메모리 구조를 제안하고 설계하였다. 제안된 메모리 구조는 빠른 동작 속도와 용량 가변성을 지원하여 공유 버퍼 ATM 스위치가 가지는 메모리 cycle time의 제한을 극복하였다. 본 메모리 구조가 지원하는 용량 가변성은 ATM 스위치에서의 교환 성능 가변성을 제공한다. 본 메모리 구조는 작은 메모리 bank들로 이루어진 2차원 배열 구조를 가진다. 메모리 용량은 부가적인 메모리 bank들을 추가하여 메모리 bank들의 배열 크기를 증가 시킴으로 인해 증가된다. 설계된 파이프라인 방식의 메모리는 4160 bit 메모리 bank를 16개 이용하여 4 × 4의 배열로 설계하였고 전체 용량은 65 Kbit이다. 레이아웃후 시뮬레이션을 통한 최대 동작 속도는 5 VV/sub dd/ 및 25℃에서 4ns이다. 설계된 메모리는 공유 가변 버퍼 ATM 스위치의 시험 설계된 칩에 내장되었다. 시험 설계된 칩은 0.6 ㎛ 2-metal 1-poly CMOS 공정 기술을 이용하여 설계하였다.

• 복식문화연구
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• 제30권3호
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• pp.403-413
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• 2022

The purpose of this study was to develop a 3D mesh-type impact protection pad with excellent motion adaptability and functionality by applying 3D printing technology. The hexagonal 3D mesh, which constitutes the basic structure of the pad, comprises two types: small and large. The bridge connecting the basic units was designed as the I-type, V-type, IV-type, and VV-type. After evaluating the characteristics of the bridge, it was found that the V-type bridge had the highest flexibility and tensile elongation. The hip joint pad and knee pad were completed by combining the hexagonal 3D mesh structure with the optimal bridge design. The impact protection pad was printed using a fused deposition modeling-type 3D printer with a filament made of thermoplastic polyurethane material, and the protection pad's performance was evaluated. When an impact force of approximately 6,500N was applied to the pad, the force attenuation percentage was 78%, and when an impact force of approximately 8,000N was applied, the force attenuation percentage was 75%. Through these results, it was confirmed that the 3D-printed impact protection pad with a hexagonal 3D mesh structure connected by a V-shaped bridge developed in this study can adapt to changes in the body surface according to movement and provides excellent impact protection performance.

• IMMUNE NETWORK
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• 제2권1호
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• pp.41-48
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• 2002

Background: Viral antigens presented on the cell surface in association with MHC class I molecules are recognized by CD8+ T cells. MHC restricted peptides are important in eliciting cellular immune responses. As peptide antigens have a weak immunigenicity, pH-sensitive liposomes were used for peptide delivery to induce effective cytotoxic T lymphocyte (CTL) responses. In the previous study, as the HBx peptides could induce specific CTLs in vitro, we tested whether the HLA-A2/$K^b$ transgenic mice that were immunized by HBx-derived peptides could be protected from a viral challenge. Methods: HBx-peptides encapsulated by pH-sensitive liposomes were prepared. $A2K^b$ transgenic mice were immunized i.m. on days one and seven with the indicated concentrations of liposome-encapsulated peptides. Three weeks later, mice were infected with $1{\times}10^7pfu$/head of recombinant vaccinia virus (rVV)-HBx via i.p. administration. The ovaries were extracted from the mice, and the presence of rVV-HBx in the ovaries was analyzed using human TK-143B cells. IFN-${\gamma}$ secretion by these cells was directly assessed using a peptide-pulsed target cell stimulation assay with either peptide-pulsed antigen presenting cells (APCs), concanavalin A ($2{\mu}g/ml$), or a vehicle. To generate peptide-specific CTLs, splenocytes obtained from the immunized mice were stimulated with $20{\mu}g/ml$ of each peptide and restimulated with peptide-pulsed APC four times. The cytotoxic activity of the CTLs was assessed by standard $^{51}Cr$-release assay and intracellular IFN-${\gamma}$ assay. Results: Immunization of these peptides as a mixture in pH-sensitive liposomes to transgenic mice induced a good protective effect from a viral challenge by inducing the peptide-specific CD8+ T cells. Mice immunized with $50{\mu}g/head$ were much better protected against viral challenge compared to those immunized with $5{\mu}g$/head, whereas the mice immunized with empty liposomes were not protected at all. After in vitro CTL culture by peptide stimulation, however, specific cytotoxicity was much higher in the CTLs from mice immunized with $5{\mu}g/head$ than $50{\mu}g/head$ group. Increase of the number of cells that intracellular IFN-${\gamma}$ secreting cell among CD8+ T cells showed similar result. Conclusion: Mice immunized with XEPs within pH-sensitive liposome were protected against viral challenge. The protective effect depended on the amount of antigen used during immunization. XEP-3-specific CTLs could be induced by peptide stimulation in vitro from splenocytes obtained from immunized mice. The cytotoxic effect of CTLs was measured by $^{51}Cr$-release assay and the percentage of accumulated intracellular IFN-${\gamma}$ secreting cells after in vitro restimulation was measured by flow cytometric analysis. The result of $^{51}Cr$-release cytotoxicity test was well correlated with that of the flow cytometric analysis. Viral protection was effective in immunized group of $50{\mu}g/head$, while in the in vitro restimulation, it showed more spectific response in $5{\mu}g$/head group.