• Title/Summary/Keyword: VV/A

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Development of a SNP marker set related to crown gall disease in grapevines by a genome wide association study

  • Kim, Dae-Gyu;Jang, Hyun A;Lim, Dong Jun;Hur, Youn Young;Lee, Kyo-Sang;Min, Jiyoung;Oh, Sang-Keun
    • Korean Journal of Agricultural Science
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    • v.47 no.3
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    • pp.693-705
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    • 2020
  • Grapes (Vitis spp. L.) are the third most produced fruit in the world. Crown gall disease caused by Agrobacterium vitis forms galls in the stems of the grapevines and reduces the vitality of the fruit trees, resulting in reduced yields. This pathogen has occurred in vineyards worldwide and caused serious economic losses. It is a soil-borne disease, so Agrobacterium vitis can survive for several years in vineyards and is difficult to control. Additionally, since there is no effective chemical control method, the most effective control method is the breeding of resistant varieties. To make the resistant variety, marker-assisted selection (MAS) enables fast breeding with low cost. In this study, we applied a genome-wide association study (GWAS), by combining phenotyping and genotyping-by-sequencing (GBS), for the development of a single nucleotide polymorphism (SNP) marker set related to crown gall disease using 350 grapevine varieties. As a result of the GBS based genotyping analysis, about 58,635 SNPs were obtained. In addition, the phenotypic analysis showed 35.2% resistance, 73% moderate susceptibility and 16.4% highly susceptibility. Moreover, after confirmation, two genes (VvARF4 and VvATL6-like) were shown to be related to crown gall disease based on the results of GWAS analysis, using the phenotypic data, and GBS. High-resolution melting analysis (HRMA) was performed using the Luna® Universal Probe with real-time PCR to distinguish the melting peaks of the resistant and susceptible varieties. Our data show that these SNP markers are expected to be helpful in evaluating resistance against grapevine crown gall disease and in breeding.

ATInSAR HOLOGRAM OBSERVATIONS OF COASTAL WAVE REFARCTION

  • Marghany, Maged
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.438-440
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    • 2003
  • This study is introducing a new approach of ATInSAR hologram for modeling wave refraction spectra pattern. TOPSAR data with L$_{-HH}$ and C-vv bands utilized spatial variation of wave refraction. Based on the phase information in along track interferometry, and ATInSAR hologram the quantitative information such swell wave height and spectra energy have been modeled. The phase information in ATInSAR hologram images can be transferred to wave refraction The ATInSAR hologram can be used to investigate the wave refraction pattern along the coastal waters. The fringe information pattern was shown to be useful in modeling wave refaction spectra varaition. The hologram interferometry wave refraction model consists of two sub-models. The purpose of first sub-model is to determine the swell wave height by using ATInSAR. Second sub-model aims to generate the holographic interferometry from the information of two wave spectra which detected by ATInSAR technique. The azimuth cut-off variations along the fringe patterns will be estimated. As azimuth cut-off contains the wave height information which could be used the significant wave height variation in convergence and divergence zone.

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A Study on the Branch Composition of an Optimum Polarization Diversity by Considering XPD in Indoor Radio Environments (실내무선 환경에서 XPD를 고려한 최적편파 다이버시티의 브랜치 구성에 관한 연구)

  • 하덕호;이주현;윤영석
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.2
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    • pp.302-311
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    • 1999
  • In this paper, in order to suggest an optimal polarization diversity composition method in indoor radio wave propagation environments, the variation of the cross polarization discrimination(XPD) was theoretically analyzed by a computer simulation and compared to the actual measured data. From the results, it can be seen that the cross polarization discrimination of the case, a circularly polarized antenna was used at the transmitting end as well as the vertical and horizontal polarized antenna branches were used at receiving end (CV-CH), is lower than that of the case, horizontal polarized antenna at the transmitting end as well as the horizontal and vertical polarized antenna branches at the receiving end(HH-HV), and that of the case, vertical polarized antenna at the transmitting end as well as the vertical and horizontal polarized antenna branches at the receiving end(VV-VH). In this paper, to get more effective CV-CH polarization diversity composition, the amount of cross polarization discrimination values at the signals received by horizontal polarized antenna is compensated and the polarization diversity effect through the cumulative probability distribution is estimated. From the evaluation results, it was found that the polarization diversity effect was better at the compensated case than at the uncompensated case. On the other hand, it can be known that the polarization diversity effect is getting better as the cross polarization discrimination values are getting lower, and also be known that the effect can be improved if a transmitting antenna is composed of the ellipse polarized antenna by adjusting the axial ratio of the circularly polarized antenna and, a receiving antenna is made up of the vertical and horizontal polarized antenna branches.

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A Study on the Method of Constructive Simulation Operation Analysis for Warfighting Experiment Supplied with the Validation Evaluation (타당성 평가가 보완된 모델 운용상의 전투실험 모의분석 절차 연구)

  • Park, Jin-Woo;Kim, Nung-Jin;Kang, Sung-Jin;Soo, Hyuk
    • Journal of the Korea Society for Simulation
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    • v.19 no.4
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    • pp.77-87
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    • 2010
  • Currently, our society has been changed from the industrial society to the information society. As the war progresses to Information Warfare, Network-Centric Warfare, Long-Range Precision Engagement and Robot Warfare, the military should advance to High-tech Scientific force. For this creation of the war potential, it is regarded as the warfighting experiment is a critical method. Surely it is rational that LVC(Live Virtual Constructive simulation) is desirable to make the warfighting experiment. But because it is limited by the cost, the time, the place and the resource, the constructive simulation(M&S : Modeling&Simulation) is a good tool to solve those problems. There are some studies about the evaluation process for developing the model, but it is unsatisfying in the process of the constructive simulations' operation. This study focuses on the way of constructive simulation operation, which is supplied with the evaluation process(VV&A : Verification Validation & Accreditation). We introduce the example of the rear area operation simulation for "appropriateness evaluation to the organization of logistic corps" by the AWAM(Army Weapon Analysis Model). This study presents the effective methods of the constructive simulations, which is based on the reliable evaluation process, so it will contribute to the warfighting experiments.

Analysis of molecular mechanism of cellular localization of various N-terminal mutants of Aplysia PDE4 in HEK293T cells (ApPDE4 long-form의 N-말단 돌연변이체들의 세포내타기팅과 타기팅 기전 분석)

  • Um, Su-Min;Jun, Yong-Woo;Kim, Kun-Hyung;Lee, Jin-A;Jang, Deok-Jin
    • Analytical Science and Technology
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    • v.29 no.1
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    • pp.10-18
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    • 2016
  • Phosphodiesterase (PDE) plays an important role in cAMP-mediated signaling within cells. We previously showed that the long-form of Aplysia PDE4 (ApPDE4) was localized in the plasma membrane and the presynaptic terminal in Aplysia sensory neurons, and the 16 N-terminal amino acid was sufficient for this targeting process. In this study, we characterized the cellular localization of various ApPDE4 mutants. We first identified the roles of each amino acid within the group of 16 N-terminal amino acids of long-form ApPDE4. As a result, we were able to identify various mutants that were localized to both the plasma membrane and the Golgi complex, Golgi only, or both the endoplasmic reticulum (ER) and the Golgi complex. To examine the role of palmitoylation on the cellular localization of ApPDE4 mutants, 2-bromo palmitate (2-BR) was used as a treatment. As a result, in the presence of 2-BR, the plasma membrane targeting of many mutants was impaired, indicating that palmitoylation was involved in the plasma membrane targeting of the mutants. We also found that PI4P play crucial roles in the Golgi targeting of (N16,C3S/VV/G)-mRFP, L(N16,C3S/LFS/R)-mRFP, and L(N16,EPL/R)-mRFP.

Extracorporeal Membrane Oxygenation in Pediatric Patients with Respiratory Failure: Early Experience with the Double-Lumen Cannula Over 2 Years

  • Kim, Woojung;Kwon, Hye Won;Min, Jooncheol;Cho, Sungkyu;Kwak, Jae Gun;Kim, Woong Han
    • Journal of Chest Surgery
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    • v.53 no.3
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    • pp.132-139
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    • 2020
  • Background: The double-lumen cannula (DLC) has begun to be used worldwide for venovenous (VV) extracorporeal membrane oxygenation (ECMO). We aimed to examine whether the DLC could be an effective tool in the treatment of pediatric respiratory failure in Korea. Methods: We reviewed the records of patients weighing under 15 kg who underwent ECMO due to respiratory failure between January 2017 and December 2018. Outcomes of ECMO using a DLC and conventional ECMO using central method or 2 peripheral cannulas were compared. Results: Twelve patients were treated with ECMO for respiratory failure. Among them, a DLC was used in 5 patients, the median age of whom was 3.8 months (interquartile range, 0.1-49.7 months). In these patients, the median values of pH, partial pressure of carbon dioxide, and partial pressure of oxygen were 7.09, 74 mm Hg, and 37 mm Hg before ECMO and corrected to 7.31, 44 mm Hg, and 85 mm Hg, respectively, after ECMO cannulation. Median blood flow rate in the patients treated with ECMO using a DLC was slightly higher than that in the conventional ECMO group, but this difference was not statistically significant (86.1 mL/kg/min and 74.3 mL/kg/min, respectively; p=1.00). One patient from the DLC group and 3 patients from the conventional group were weaned off ECMO. Conclusion: VV ECMO using a DLC provided adequate oxygenation, ventilation, and blood flow rate in Korean pediatric patients with respiratory failure. Further prospective and randomized studies are warranted.

Measurement of Backscattering Coefficients of Rice Canopy Using a Ground Polarimetric Scatterometer System (지상관측 레이다 산란계를 이용한 벼 군락의 후방산란계수 측정)

  • Hong, Jin-Young;Kim, Yi-Hyun;Oh, Yi-Sok;Hong, Suk-Young
    • Korean Journal of Remote Sensing
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    • v.23 no.2
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    • pp.145-152
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    • 2007
  • The polarimetric backscattering coefficients of a wet-land rice field which is an experimental plot belong to National Institute of Agricultural Science and Technology in Suwon are measured using ground-based polarimetric scatterometers at 1.8 and 5.3 GHz throughout a growth year from transplanting period to harvest period (May to October in 2006). The polarimetric scatterometers consist of a vector network analyzer with time-gating function and polarimetric antenna set, and are well calibrated to get VV-, HV-, VH-, HH-polarized backscattering coefficients from the measurements, based on single target calibration technique using a trihedral corner reflector. The polarimetric backscattering coefficients are measured at $30^{\circ},\;40^{\circ},\;50^{\circ}\;and\;60^{\circ}$ with 30 independent samples for each incidence angle at each frequency. In the measurement periods the ground truth data including fresh and dry biomass, plant height, stem density, leaf area, specific leaf area, and moisture contents are also collected for each measurement. The temporal variations of the measured backscattering coefficients as well as the measured plant height, LAI (leaf area index) and biomass are analyzed. Then, the measured polarimetric backscattering coefficients are compared with the rice growth parameters. The measured plant height increases monotonically while the measured LAI increases only till the ripening period and decreases after the ripening period. The measured backscattering coefficientsare fitted with polynomial expressions as functions of growth age, plant LAI and plant height for each polarization, frequency, and incidence angle. As the incidence angle is bigger, correlations of L band signature to the rice growth was higher than that of C band signatures. It is found that the HH-polarized backscattering coefficients are more sensitive than the VV-polarized backscattering coefficients to growth age and other input parameters. It is necessary to divide the data according to the growth period which shows the qualitative changes of growth such as panicale initiation, flowering or heading to derive functions to estimate rice growth.

Analysis of Backscattering Coefficients of Corn Fields Using the First-Order Vector Radiative Transfer Technique (1차 Vector Radiative Transfer 기법을 이용한 옥수수 생육에 따른 후방산란 특성 분석)

  • Kweon, Soon-Koo;Hwang, Ji-Hwan;Park, Sin-Myeong;Hong, Sungwook;Oh, Yisok
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.25 no.4
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    • pp.476-482
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    • 2014
  • In this study, we analyzed the effect of corn growth on the radar backscattering coefficient. At first, we measured the backscattering coefficients of various corn fields using a polarimetric scatterometer system. The backscattering coefficients of the corn fields were also computed using the 1st-order VRT(Vector Radiative Transfer) model with field-measured input parameters. Then, we analyzed the experimental and numerical backscattering coefficients of corn fields. As a result, we found that the backscatter from an underlying soil layer is dominant for early growing stage. On the other hand, for vegetative stage with a higher LAI(Leaf-Area-Index), the backscatter from vegetation canopy becomes dominant, and its backscattering coefficients increase as incidence angle increases because of the effect of leaf angle distribution. It was also found that the estimated backscattering coefficients agree quite well with the field-measured radar backscattering coefficients with an RMSE(Root Mean Square Error) of 1.32 dB for VV-polarization and 0.99 dB for HH-polarization. Finally, we compared the backscattering characteristics of vegetation and soil layers with various LAI values.

A Comparison of the Amount of Fluoride Ion Released and Remineralization Effect on the Initial Caries Lesion of the Various Fluoride Varnishes (시판중인 불소 바니쉬의 불소이온 유리량 및 초기우식병소의 재광화 효과 비교)

  • Lee, Kayoung;Lee, Sangho;Lee, Nanyoung;Jih, Myeongkwan
    • Journal of the korean academy of Pediatric Dentistry
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    • v.43 no.4
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    • pp.443-451
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    • 2016
  • Subjects of this study were : $FluoroDose^{(R)}$ (FD, Centrix Inc., USA), $Enamelast^{TM}$ (EL, Ultradent Product Inc., USA), $Clinpro^{TM}$ white varnish (CW, 3M ESPE, USA), $CavityShield^{TM}$ (CS, 3M ESPE, USA), V $varnish^{TM}$ (VV, Vericom, Korea), MI $varnish^{TM}$ (MI, GC, Japan). The amount of fluoride ion release was measured eight times during 168 hours to see change in accumulation with the course of time using a measuring instrument. And the remineralization rate was measured with Quantitative Light-induced Fluorescence (QLF). V $varnish^{TM}$ group and MI $varnish^{TM}$ group showed high remineralization rates with statistically significance while $CavityShield^{TM}$ group was the lowest rate of remineralization (p < 0.05). After that, several chosen samples were scanned through electron microscope (SEM). Demineralized enamel was observed as the number of enamel crystal was very small; enamel rods and crystals were highly protruding. Remineralized groups with fluoride varnishes show the decreasing tendency of the surface roughness compared to the demineralized enamel.

Analysis of Relationships between Features Extracted from SAR Data and Land-cover Classes (SAR 자료에서 추출한 특징들과 토지 피복 항목 사이의 연관성 분석)

  • Park, No-Wook;Chi, Kwang-Hoon;Lee, Hoon-Yol
    • Korean Journal of Remote Sensing
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    • v.23 no.4
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    • pp.257-272
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    • 2007
  • This paper analyzed relationships between various features from SAR data with multiple acquisition dates and mode (frequency, polarization and incidence angles), and land-cover classes. Two typical types of features were extracted by considering acquisition conditions of currently available SAR data. First, coherence, temporal variability and principal component transform-based features were extracted from multi-temporal and single mode SAR data. C-band ERS-1/2, ENVISAT ASAR and Radarsat-1, and L-band JERS-1 SAR data were used for those features and different characteristics of different SAR sensor data were discussed in terms of land-cover discrimination capability. Overall, tandem coherence showed the best discrimination capability among various features. Long-term coherence from C-band SAR data provided a useful information on the discrimination of urban areas from other classes. Paddy fields showed the highest temporal variability values in all SAR sensor data. Features from principal component transform contained particular information relevant to specific land-cover class. As features for multiple mode SAR data acquired at similar dates, polarization ratio and multi-channel variability were also considered. VH/VV polarization ratio was a useful feature for the discrimination of forest and dry fields in which the distributions of coherence and temporal variability were significantly overlapped. It would be expected that the case study results could be useful information on improvement of classification accuracy in land-cover classification with SAR data, provided that the main findings of this paper would be confirmed by extensive case studies based on multi-temporal SAR data with various modes and ground-based SAR experiments.