• Asian-Australasian Journal of Animal Sciences
• /
• v.32 no.3
• /
• pp.421-429
• /
• 2019

Objective: In this study, the effects of vacuum (VP) and high-oxygen modified atmosphere ($80%\;O_2+20%\;CO_2$) packaging (HiOx-MAP) on physico-chemical and microbiological properties of minced water buffalo meat were investigated. Methods: After minced meat preparation, samples were packaged under VP and HiOx-MAP and stored at $2^{\circ}C{\pm}0.5^{\circ}C$ for 14 days. Samples taken on certain days were subjected to total aerobic mesophilic bacteria, total aerobic psychrotrophic bacteria, lactic acid bacteria, Pseudomonas, Enterobacteriaceae and yeast-mold counts as well as pH, color ($L^*$, $a^*$, and $b^*$) and thiobarbituric acid reactive substances (TBARS) analyses. Results: In minced water buffalo meat packaged under HiOx-MAP, TBARS value exceeded 1 mg malondialdehyde/kg on the 4th day of the storage. In VP samples, TBARS value remained close to initial TBARS value during storage. According to the findings, $a^*$ value was determined to be high in the HiOx-MAP samples within initial days of the storage. However, no significant changes in $a^*$ value were observed in VP samples during storage. In contrast, the mean value of $L^*$ was detected as higher in HiOx-MAP sample than VP samples. The count of psychrotrophic bacteria increased more than that of mesophilic bacteria during storage. The growth of Enterobacteriaceae and Pseudomonas was delayed in both the packaging methods. However, lactic acid bacteria exhibited more growth in VP samples compared to MAP samples. Conclusion: The lipid oxidation proceeded faster than expected in minced water buffalo meat packed with HiOx-MAP method. This situation adversely affected the $a^*$ value. On the other hand, similar microbiological results were obtained in both packing methods.

• Restorative Dentistry and Endodontics
• /
• v.48 no.2
• /
• pp.16.1-16.11
• /
• 2023

Objectives: The aim of this study was to evaluate the influence of peak kilovoltage (kVp) and a metal artifact reduction (MAR) tool on image quality and the diagnosis of vertical root fracture (VRF) in cone-beam computed tomography (CBCT). Materials and Methods: Twenty single-rooted human teeth filled with an intracanal metal post were divided into 2 groups: control (n = 10) and VRF (n = 10). Each tooth was placed into the socket of a dry mandible, and CBCT scans were acquired using a Picasso Trio varying the kVp (70, 80, 90, or 99), and the use of MAR (with or without). The examinations were assessed by 5 examiners for the diagnosis of VRF using a 5-point scale. A subjective evaluation of the expression of artifacts was done by comparing random axial images of the studied protocols. The results of the diagnoses were analyzed using 2-way analysis of variance and the Tukey post hoc test, the subjective evaluations were compared using the Friedman test, and intra-examiner reproducibility was evaluated using the weighted kappa test (α = 5%). Results: The kVp and MAR did not influence the diagnosis of VRF (p > 0.05). According to the subjective classification, the 99 kVp protocol with MAR demonstrated the least expression of artifacts, while the 70 kVp protocol without MAR led to the most artifacts. Conclusions: Protocols with higher kVp combined with MAR improved the image quality of CBCT examinations. However, those factors did not lead to an improvement in the diagnosis of VRF.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• v.13 no.2
• /
• pp.134-145
• /
• 2010

Purpose: The serologic diagnosis of rotaviral infections is not commonly used in clinical practice, but is used in seroepidemiologic studies. In this study, the usefulness of Escherichia coli-expressed recombinant VP6 proteins of group A rotavirus in the serodiagnosis of rotavirus infections by ELISA was evaluated. Methods: The recombinant VP6 proteins of group A rotavirus expressed in E. coli Rosetta II strain were purified and identified. One hundred sera from 22 children (4 healthy neonates, 13 healthy children, and 5 immunocompromised children) who had serial sera samples prior to and after rotavirus infections were provided by the Gyeongsang National University Hospital, a member of the National Biobank of Korea. IgG, IgA, and IgM antibodies against rVP6 were analyzed by ELISA in all of the patients and Western blot analysis in 4 neonates. Results: ELISA tests using rVP6 proteins of group A rotavirus as antigen revealed that IgG, IgA, and IgM antibodies increased after rotaviral infections in most neonates and healthy children. IgG antibodies also increased after rotaviral infections in most immunocompromised children without an adequate increase in IgM or IgA antibodies. Western blot analysis in four neonates revealed very early IgM antibody responses, even in the sera with low optical densities in ELISA tests. Conclusion: Our study showed that ELISA using rVP6 as an antigen is a valid diagnostic tool for seroepidemiologic studies of rotavirus infections and Western blot analysis is a sensitive test in detecting IgG, IgA, and and IgM antibodies in patients with rotavirus infections.

• Journal of the Korean Society of Safety
• /
• v.23 no.1
• /
• pp.66-71
• /
• 2008

A new route to phthalocyanine complexes were developed to synthesize these products by fusion in the absence of solvent. This new method of synthesis without using solvent has advantages over the conventional synthetic methods since there are no risk of explosion and formation of harmful vapor from organic solvent. Reaction of PcFe with axial ligands such as $PcFe(4-VP)_2$[Pc: Phthalocyanine, 4-VP: 4-Vinylpyridine] and $PcFe(VIM)_2$[VIM: 1-Vinylimidazole] afforded powderlike, pure dark greenish blue colored products. The resulted products are soluble in $CH_2Cl_2$ and found to be complexes of the type $PcFeL_2$. Spectral properties were studied with ATR-FTIR and UV/Vis. Thermal and electrical characterization was also performed. Phthalocyanine complexes exhibit useful properties such as UV/Vis blocking, antistatic characteristics and excellent thermal stability and we anticipate various applicability in numerous products.

• Journal of radiological science and technology
• /
• v.13 no.2
• /
• pp.31-36
• /
• 1990

We got the following results from the experiment and examination in order to measure the bone-marrow dose of the patients when we did chest or abdomen radiography in the hospitals located in Seoul City from Jan. 1989 until Feb. 1990. 1. In the exposure factors for chest radiography, tube voltage $60{\sim}69\;kVp$ took 48.3%, $80{\sim}89\;or\;90{\sim}99\;kVp$ took 13.8% respectively, $70{\sim}79\;kVp$ 10.3% and $100{\sim}129\;kVp$ 10.3%. In tube current and exposure times, $6{\sim}10\;mAs$ took 41.4%, $16{\sim}20\;mAs$ took 20.7% and $11{\sim}15\;mAs$ 13.8%, measure under 5mAs 10.4% orderly. 2. In chest radiography, the bone-marrow dose came to the minimum 3.48 mrad, to the maximum 35.67 mrad, to the mean 14.46 mrad, to the standard deviation 8.89 mrad. 3. Comparing bone-marrow doses of the patients when we used Bucky technique and non-Bucky technique, that of Bucky technique was very higher than that of non-Bucky technique. Because the result was that Bucky technique had the span of $6.09{\sim}35.67$ mrad, while non-Bucky technique had the span of $3.48{\sim}17.40$ mrad. 4. In the exposure factors for abdomen radiography, tube voltage of $70{\sim}79\;kVp$ was 63.0%, that of $80{\sim}89\;kVp$ was 22.2%, that of $60{\sim}69\;kVp$ was 11.1 %. Tube current and exposure times of $31{\sim}40\;kVp$ was 33.4%, that of $51{\sim}60\;mAs$ was 29.6% and that of $41{\sim}50\;mAs$ was 22.2%. 5. In abdomen radiography, the bone-marrow dose of the patients came to the minimum of 6.96 mrad, to the maximum of 60.90 mrad, to the mean of 35.73mrad, to the standard deviation of 12.65 mrad.

• Journal of the Korean Society of Radiology
• /
• v.13 no.5
• /
• pp.749-755
• /
• 2019

In this study, we investigated the effects of tube voltage (kVp) and tube current (mAs) on an exposure index (EI) applied to a digital radiography. There used an inverter type digital X-ray generator and an image receptor (IR) utilized a portable wireless detector without a grid. Radiographic images were acquired using a cone pyramid phantom produced using a 3D printer. The X-ray tube voltage was increased from 40 kVp to 120 kVp with 10 kVp increment, and the tube current was increased from 1 mAs to 128 mAs with the twice increment. As a result, kVp had logarithmic relationship with the EI as high $R^2$ value, and mAs had a very high linear relationship too. Also, there was a high correlation between the area dose and the EI of the IR, with the $R^2$ value being 0.76 or more. In conclusion, it had showed that mAs affected the EI linearly, and that it could be advantageous to adjust the easy-to-predict mAs to maintain proper image qualities.

• IMMUNE NETWORK
• /
• v.13 no.4
• /
• pp.157-162
• /
• 2013

Application of vaccine materials through oral mucosal route confers great economical advantage in animal farming industry due to much less vaccination cost compared with that of injection-based vaccination. In particular, oral administration of recombinant protein antigen against foot-and- mouth disease virus (FMDV) is an ideal strategy because it is safe from FMDV transmission during vaccine production and can induce antigen-specific immune response in mucosal compartments, where FMDV infection has been initiated, which is hardly achievable through parenteral immunization. Given that effective delivery of vaccine materials into immune inductive sites is prerequisite for effective oral mucosal vaccination, M cell-targeting strategy is crucial in successful vaccination since M cells are main gateway for luminal antigen influx into mucosal lymphoid tissue. Here, we applied previously identified M cell-targeting ligand Co1 to VP1 of FMDV in order to test the possible oral mucosal vaccination against FMDV infection. M cell-targeting ligand Co1-conjugated VP1 interacted efficiently with M cells of Peyer's patch. In addition, oral administration of ligand-conjugated VP1 enhanced the induction of VP1-specific IgG and IgA responses in systemic and mucosal compartments, respectively, in comparison with those from oral administration of VP1 alone. In addition, the enhanced VP1-specific immune response was found to be due to antigen-specific Th2-type cytokine production. Collectively, it is suggested that the M cell-targeting strategy could be applied to develop efficient oral mucosal vaccine against FMDV infection.

• Progress in Medical Physics
• /
• v.6 no.2
• /
• pp.3-12
• /
• 1995

A 50 $\mu\textrm{m}$ thick niobium filter and its quantitatively determined aluminum equivalent filter were evaluated for effects on entrance skin dose, image quality, and x-ray tube loading for three different tube voltages in radiology. There was no significant difference in the reducion in entrance skin dose and increase in tube loading between two filters while keeping radiographic contrast on the film. For the clinical use of the aluminum equivalent filter as an alternative to the niobium filter in radiology, aluminum equivalent filter thickness at the mid energy range of radiology, 90 kVp, was measured and the filter was applied to the other kVp values, 73 and 125 kVps, to evaluate the effect on the entrance skin dose and tube loading. There was no significant difference between two filter cases at the selected kVp. The aluminum filter with equivalent thickness can be used as an inexpensive alternative to the niobium filter.

• Fashion & Textile Research Journal
• /
• v.2 no.5
• /
• pp.398-404
• /
• 2000

We examined the effect of individual sweating responses on thermoregulatory responses induced by heat of sorption, immediately after the onset of sweating. The present study consists of two experiments. In experiment 1, made of 100% cotton (C) and 100% polyester (P) clothing were exposed in the chamber at ambient temperature (Ta) of $27.2^{\circ}C$ and relative humidity (rh) raised from 50% to 95% at five different increase rates of environmental vapor pressure (VP). The increase rate of clothing surface temperature (Tcs), peak Tcs and peak time showed significant correlation with the increase rate of environmental VP in C-clothing (p<0.05). In experiment 2, seven female subjects were studied during leg water immersion ($35-41^{\circ}C$) for 70min in Ta of 27.2 and 50%rh. There were significant positive correlations in the increase rate of clothing microclimate VP vs. changes in Tcs, skin blood flow, mean skin temperature and mean body temperature (p<0.05). The present results showed that individual clothing microclimate VP had significant effects on thermoregulatory responses induced by heat of sorption wearing C ensembles.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
• /
• v.22 no.2
• /
• pp.293-303
• /
• 1992

Generally the patient's absorb dose and readability of radiograms are affected by the exposure time and kVp of which are related with the radiographic density and contrast The investigator carried studies to know the adequate level of exposure time and kVp to obtain the better readability of radiograms. In these studies dried human mandible with selfcuring acrylic resins attached with aluminum step wedge was used and readability of radiograms were compared with each other by videodensitometry among various combination sets of the exposure time, such as 5, 6, 8, 12, 15, 19, 24, 30, 38, 48 and 60, and varing level of kVp, such as 60, 65, 70, 80 and 90 respectively. The obtained results were as follows: 1. As exposure time and kVp were increased, radiographic density of radiograms was increased. 2. The subject contrast was increased where aluminum step wedge was thin and reduced in the reversed condition. At the thin aluminum step wedge, subject contrast was increased at the condition of lower kilovoltage than that of higher kilovoltage. 3. In the case of non-constant radiographic density, the radiographic contrast was reduced with the increment kilovoltage. The radiographic contrast was increased in the lower kilovoltage with the longer exposure time and the higher kilovoltage with the shorter exposure time. 4. At the condition of short exposure time, better readability of each reading item was obtained with the increment of the kilovoltage but at the opposite condition increasing exposure time worsened readability of radiograms. Since X-ray machine in the current dental clinics is fixed between the range of 60-70kVp and 10mA, good radiograms can be obtained by varied exposure time. But according to the conclusion of these studies, better radiograms can be obtained by using filtered high kVp and then the absorb dose to patient and exposure time can be reduced.