• Title/Summary/Keyword: VP1

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Typing and Sequence Analysis of the VP7 Gene of Rotavirus Isolated from Infants in Korea (한국영아에서 분리된 로타바이러스의 VP7 유전자형 및 염기서열 분석)

  • Song, Mi-Ok;Yun, Karyn;Chung, Sang-In;Choi, Chul-Soon;Lim, In-Seok;Kang, Shien-Young;An, Chang-Nam;Kim, Won-Yong
    • The Journal of Korean Society of Virology
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    • v.30 no.2
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    • pp.101-112
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    • 2000
  • Rotaviruses are the most common cause of severe vomiting and diarrhea in children worldwide and classified as a genus in the family Reoviridae. Rotavirus has eleven segmented dsRNAs and the virion consists of three shells. Outer capsid VP7 and VP4 induce neutralizing antibodies and are classified into G types (glycoprotein VP7) and P types (protease-sensitive VP4). Characterization of VP7 gene of Korean isolates of human rotavirus was performed using multiplex PCR and nucleotide sequence analysis. After RT-PCR amplification of full length (1,062 bp) of VP7 genes, the amplified PCR products were G typed by multiplex PCR and the nucleotide sequences were compared with those of reference rotavirus from GenBank. The G type analysis revealed that 25% (2/8) belong to G1, whereas 37.5% (3/8) benong to G2 and G4, respectively. The Korean isolates within the same serotypes showed high homology of nucleotide sequences and could be discriminated from foreign isolates exception with two strains (CAU009 and CAU022). But Korean isolates CAU009 and CAU022 were close related into japanease isolates 417 (99.2%) and indian isolates (97.6%) than Korean isolatese. Our results showed that these two strains were supposed to be originated from abroad. As a results, The G typing and nucleotide sequence analysis of VP7 gene of rotavirus isolated from infants in Korea could be used for identification, serotying and determination of novel or unusual strains of rotaviruses.

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The Activity of Standard and Fast SCR over V-based Catalysts Supported on Various TiO2 (다양한 TiO2에 담지된 바나디아 촉매의 표준 및 빠른 SCR 활성)

  • Ji Eun Jeong;Yeon Jeong Jo;Inyoung Lee;Jeongkeun Lee;Chang-Yong Lee
    • Applied Chemistry for Engineering
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    • v.34 no.6
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    • pp.584-589
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    • 2023
  • The physicochemical properties of VOx/TiO2 catalysts with different TiO2 supports were analyzed, and SCR reactions were performed. VOx/TiO2 catalysts were prepared by impregnation using anatase TiO2, which was manufactured by Sigma Aldrich and prepared from TiOCl2 and titanium isopropoxide (TTIP) as a precursor. They are denoted as VS, VC, and VP. The specific surface area of the VS was 1/10 or less of that of the VC and VP, and the dispersibility of vanadium oxide was relatively low. As a result of XPS analysis, the ratio of adsorbed oxygen was higher in VS and VP with Ti3+ than in VC. In VC and VP, vanadium mainly existed in V4+ and V3+ states in relation to the dispersibility of vanadium oxide. The amount of adsorbed oxygen contributed more to NH3-SCR activity than vanadium oxide dispersibility below 250 ℃, while vanadium oxide dispersibility contributed more to activity beyond 300 ℃. The fast SCR activity in all three samples was the highest at NO2/NOx = 0.5, followed by VS < VC < VP samples. It was determined that the dispersibility of vanadium oxide had a significant effect on fast NH3-SCR activity.

Effects of Exposure Dose Reduction by Optimization of Automatic Exposure Control Factors in Digital Radiographic Examination of Paranasal Sinus (부비동 디지털 엑스선검사에서 자동노출제어 조절인자의 최적화를 통한 조사선량 감소 효과)

  • Jeong, Min-Gyu;Seoung, Youl-Hun
    • Journal of radiological science and technology
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    • v.44 no.3
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    • pp.173-181
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    • 2021
  • The purpose of this study was to reduce dose while maintaining image quality during digital radiographic examination of paranasal sinus by using the automatic exposure control (AEC) system. The tube voltage was set as six stages that increased by about 10 kVp to 70 kVp, 81 kVp, 90 kVp, 102 kVp, 109 kVp and 117 kVp. And then the AEC system conditions were consisted of 9 setting environments, that change mode of the sensitivity (S200, S400, S800) and the density (+2.5, 0, -2.5). We measured automatically exposed tube current (mAs) under 54 conditions with combined these, and assessed SNR and PSNR through the acquired images. In addition, four radiologists performed a qualitative assessment of the acquired images for each combination on a five-point scale of the Likert. As a result, the lowest dose and the highest values of SNR and PSNR in images with a qualitative assessment more than 4 point were the AEC control factors of 90 kVp, S800, D2.5. We applied this condition to the clinical trial, it showed an effect of 83.1% reduction in exposure radiation dose (mR). Therefore, AEC system could be used as dose reduction technology if it understood and used related regulatory factors and physical characteristics.

A Study on Optimum kVp in Four Spot Films of Stomach Examinations (위(胃) 사등분할촬영시(四等分割撮影時) 적정(適正)한 관전압(管電壓)에 관(關)한 검토(檢討))

  • Kim, Wha-Gon;Kyong, Kwang-Hyon;Kim, Young-Hwan;Huh, Joon
    • Journal of radiological science and technology
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    • v.3 no.1
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    • pp.37-41
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    • 1980
  • In an attempt to provide optimum kVp for four spot films of stomach examinations, we measured experimentally film density and scatter radiation with field size. And to investigate the effect of concentrations in barium sulphite and kVp in spot films of stomach fluoroscopy were carried out and the following results were obained. 1. The entire density of film by field size has the sharpest increase from $10cm^2$ to $100cm^2$, and relatively flattened curve beyond $500cm^2$ in field size. 2. The quantity of scatter radiation reaching an X-ray film depends upon field size: the larger the fields, the more scatter radiation. 3. It is necessary for increasing 3 to 5kVp as for the absence of barium sulphite and 5 to 7 kVp in the case of 20 per cent and 25 per cent in barium sulphite concentrations to produce uniform density in the four spot films for stomach fluoroscopy.

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Leveraging and Fostering Diversity in the IS Discipline: Intradisciplinary Knowledge Building via the IT View-IS Phenomenon (VP) Matrix

  • Inchan Kim;Jama Summers
    • Asia pacific journal of information systems
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    • v.34 no.1
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    • pp.49-90
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    • 2024
  • Intradisciplinary research refers to research that integrates ideas often associated with different research domains in a discipline. Such cross-fertilization leverages abundant diversity present in the IS discipline to tackle increasingly complex IS problems and grand challenges. Despite its importance and recent attention, a concerted, sustained effort toward intradisciplinary research is lagging. A fundamental issue we see is a lack of an elaborate IS research map that effectively shows similarities and differences among research domains and demonstrates types of ideas that may travel and integrate into different domains. We thus aim to propose an elaborate IS research map and compile research elements that can be tried and combined across research domains. To do so, we utilize two IS classics (i.e., IT views and IS phenomena), identify their complementarity, and interweave the two disparate ways of portraying the IS research field. The resultant view-phenomenon (VP) matrix specifies research domains based on two consistent, comprehensive criteria and helps researchers discern similarities and differences among research domains more effectively. The VP matrix also sheds light on a variety of research elements that can flow across research domains. The VP matrix along with the research elements together facilitate intradisciplinary efforts and, more broadly, help the IS discipline to prosper. The VP matrix is particularly helpful for doctoral students and young scholars.

Effects of Gas Composition in the Modified Atmosphere Packaging on the Shelf-life of Longissimus dorsi of Korean Native Black Pigs-Duroc Crossbred during Refrigerated Storage

  • Muhlisin, Muhlisin;Panjono, Panjono;Kim, Dong Soo;Song, Yeong Rae;Lee, Sung-Jin;Lee, Jeong Koo;Lee, Sung Ki
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.8
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    • pp.1157-1163
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    • 2014
  • This study was conducted to observe the effects of gas composition in modified atmosphere packaging (MAP) on the shelf-life of Longissimus dorsi of Korean Native Black Pigs-Duroc Crossbred ($KNP{\times}D$) during refrigerated storage. Muscle sample was obtained from the left side of carcass of seven months old of $KNP{\times}D$ barrow. The sample was sliced into 1 cm in thickness, placed on trays (two slices/tray) and filled with different gas composition, i.e. 0:20:80/$O_2:CO_2:N_2$ (MAP1), 30:20:50/$O_2:CO_2:N_2$ (MAP2) and 70:20:10/$O_2:CO_2:N_2$ (MAP3). Other slices of sample were vacuum packed (VP) as a control. All packs were stored at $5{\pm}1^{\circ}C$. At 12 d of storage, pH value of MAP2 and MAP3 were higher (p<0.05) than that of MAP1 and pH value of MAP1 was higher (p<0.05) than that of VP. At 6 d of storage, redness ($a^*$) value of MAP2 and MAP3 were higher (p<0.05) than that of VP and MAP1 and, at 9 and 12 d of storage, redness value of MAP3 was higher (p<0.05) than that of VP, MAP1, and MAP2. At 3, 6, 9, and 12 d of storage, the 2- thiobarbituric acid reactive substances (TBARS) value of MAP3 was higher than that of MAP2 and TBARS value of MAP2 was higher than that of VP and MAP1. At 3, 6, 9, and 12 d of storage, volatile basic nitrogen values of MAP2 and MAP3 were higher (p<0.05) than those of VP and MAP1. At 3 d of storage, total aerobic plate counts of MAP2 and MAP3 were higher (p<0.05) than those of VP and MAP1 and, at 6 d of storage, total aerobic plate counts of MAP3 was higher (p<0.05) than that of MAP1 and MAP2. However, there was no significant different total aerobic plate count among MAP1, MAP2, and MAP3 at 9 and 12 d of storage. There was no significant different total anaerobic plate count among MAP1, MAP2, and MAP3 during storage. It is concluded that the MAP containing 30:20:50/$O_2:CO_2:N_2$ gas composition (MAP2) might be ideal for better meat quality for $KNP{\times}D$ meat.

The unique role of domain 2A of the hepatitis A virus precursor polypeptide P1-2A in viral morphogenesis

  • Morace, Graziella;Kusov, Yuri;Dzagurov, Georgy;Beneduce, Francesca;Gauss-Muller, Verena
    • BMB Reports
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    • v.41 no.9
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    • pp.678-683
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    • 2008
  • The initial step during assembly of the hepatitis A virus particle is driven by domain 2A of P1-2A, which is the precursor of the structural proteins. The proteolytic removal of 2A from particulate VP1-2A by an as yet unknown host enzyme presumably terminates viral morphogenesis. Using a genetic approach, we show that a basic amino acid residue at the C-terminus of VP1 is required for efficient particle assembly and that host proteases trypsin and cathepsin L remove 2A from hepatitis A virus particles in vitro. Analyses of insertion mutants in the C-terminus of 2A reveal that this part of 2A is important for liberation of P1-2A from the polyprotein. The data provide the first evidence that the VP1/2A junction is involved in both viral particle assembly and maturation and, therefore, seems to coordinate the first and last steps in viral morphogenesis.

Camera Modelling of Linear Pushbroom Images - Quality analysis of various algorithms (대표적 위성영상 카메라 모델링 알고리즘들의 비교연구)

  • 김태정;김승범;신동석
    • Korean Journal of Remote Sensing
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    • v.16 no.1
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    • pp.73-86
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    • 2000
  • Commonly-used methods for camera modelling of pushbroom images were implemented and their performances were assessed. The models include Vector Propagation) model, Gugan and Downman(GD)'s model, Orun and Natarajan(ON)'s model, and Direct Linear Transformation(DLT) model The models were tested on a SPOT full-scene over Seoul. The number of ground control points(GCP) used range from 1 to 23. For less than 6 GCPs all other models fail except VP, with VP's accuracy being 2.7 pixels. With mode than 6 GCPs ON shows the best accuracy with 1pixel accuracy while the accuracy of VP is 1.5 pixels. GD fails in most cases due to the correlation among model parameters. The accuracy of DLT does not converge but fluctuates between 1 and 4 pixels subject to GCPs used. VP has an advantage in that its results can be used for the estimation of satellite orbit. Unresolved topics are: to remove errors in GCPs from the aforementioned accuracy value; to improve the performance of VP.

A Study on Exposure Technics in Roentgenography of the Stomach (위조영촬영시(胃造影撮影時) 촬영조건(撮影條件)에 관(關)한 연구(硏究))

  • Kyong, Kwang-Hyon;Kim, Heung-Tai;Kwon, Dal-Kwan;Sun, Han-Kyung;Huh, Joon
    • Journal of radiological science and technology
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    • v.4 no.1
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    • pp.45-53
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    • 1981
  • In examinations of the stomach roentgenography, it is imperative to obtain adequate film density throughout all its different regions. Therefore, it is necessary to use more sophiscated exposure techniques. In order to achieve these purpose, the radiologic technologists must be measured abdominal thickness in variations with patient positions. In consideration of these problem, the author was made an experiment on correction method of kVp and mAs by abdominal thickness in roentgenography of the stomach. The results were summarized as follws: 1. When the patient in erect position, abdominal thickness was the most thickened at the level of 3cm inferior to umbilicus without regard to body habitus and it was the most thickened at the level of 3cm superior to umbilicus in prone and supine position. 2. As a result of measuring film density for stomach, the adequate film density was represented from 0.70 to 2.49 in erect position and $0.28{\sim}1.18$ in supine position, $0.5{\sim}2.45$ in prone position. 3. In order to obtain uniform film density in 1.25, the correction factor for kVp by abdominal thickness was represented average ${\pm}4.5kVp\;per\;{\pm}1cm$ in a fixed 50 mAs, and average ${\pm}3.9kVp\;per\;{\pm}1cm$ in a fixed 100mAs. 4. In order to obtain uniform film density in 1.25, the correction factor for mAs by abdominal thickness was represented average ${\pm}30.9%\;per\;{\pm}1cm$ in a fixed 80 kvp and ${\pm}26.9%\;per\;{\pm}1cm$ in a fixed 100kVp.

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A Fifteen-year Epidemiological Study of Ventriculoperitoneal Shunt Infections in Pediatric Patients: A Single Center Experience (소아 환자에서의 뇌실-복강 단락 감염의 역학적 고찰: 15년 간의 단일 기관 연구)

  • Kim, Yeon Kyung;Shin, Hyung Jin;Kim, Yae Jean
    • Pediatric Infection and Vaccine
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    • v.19 no.3
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    • pp.141-148
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    • 2012
  • Purpose : Ventriculoperitoneal (VP) shunt insertion is an important treatment modality in children with hydrocephalus. VP shunt infection is a major complication and an important factor that determines the surgery outcome. This 15-year study was performed to evaluate the epidemiology of VP shunt infections in pediatric patients treated at our center. Methods : A retrospective review of medical records was performed in patients 18 years old or younger who underwent VP shunt insertion surgery from April 1995 to June 2010. Results : Three hundred twenty-seven VP shunt surgeries were performed in a total of 190 pediatric patients (83 females, 107 males). The median age of the patients was 2.4 years (range, 0.02-17.9 years). Having a malignant brain tumor was the most frequent cause for VP shunt insertion. The shunt infection rate was 6.7% (22/327) per 100 operations and 9.5% (18/190) per 100 patients, and the incidence rate was 0.45 infection cases per 100 shunt operations-year. The most common pathogen was coagulase-negative staphylococcus (n=7) followed by methicillin resistant Staphylococcus aureus (n=1). Ten cases were treated with vancomycin and beta-lactam antibiotic (cephalosporin or carbapenem) combination therapy and 7 cases were treated with vancomycin monotherapy. The median duration of antibiotic treatment was 26 days (range, 7 to 58 days). Surgical intervention was performed in 18 cases (18/22, 81.8%). Conclusion : Epidemiologic information regarding VP shunt infections in pediatric patients is valuable that will help guide proper antibiotic management. Additional studies on the risk factors for developing VP shunt infections are also warranted.

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