• Title/Summary/Keyword: V-bed

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Protein Adsorption and Hydrodynamic Stability of a Dense, Pellicular Adsorbent in High-Biomass Expanded Bed Chromatography

  • Chow, Yen Mei;Tey, Beng Ti;Ibrahim, Mohd Nordin;Ariff, Arbakariya;Ling, Tae Chuan
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.3
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    • pp.268-272
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    • 2006
  • A dense, pellicular UpFront adsorbent ($p=1.5 g/cm^3$, UpFront Chromatography, Cophenhagen, Denmark) was characterized in terms of hydrodynamic properties and protein adsorption performance in expanded bed chromatography. Cibacron Blue 3GA was immobilised into the adsorbent and protein adsorption of bovine serum albumin (BSA) was selected to test the setup. The Bodenstein number and axial dispersion coefficient estimated for this dense pellicular adsorbent was 54 and $1.63{\times}10^{-5}m^2/s$, respectively, indicating a stable expanded bed. It could be shown that the BSA protein was captured by the adsorbent in the presence of 30% (w/v) of whole-yeast cells with an estimated dynamic binding capacity $(C/C_o = 0.01)$ of approximately 6.5 mg/mL adsorbent.

THERMAL AND STRUCTURAL ANALYSIS OF CALANDRIA VESSEL OF A PHWR DURING A SEVERE ACCIDENT

  • Kulkarni, P.P.;Prasad, S.V.;Nayak, A.K.;Vijayan, P.K.
    • Nuclear Engineering and Technology
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    • v.45 no.4
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    • pp.469-476
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    • 2013
  • In a postulated severe core damage accident in a PHWR, multiple failures of core cooling systems may lead to the collapse of pressure tubes and calandria tubes, which may ultimately relocate inside the calandria vessel forming a terminal debris bed. The debris bed, which may reach high temperatures due to the decay heat, is cooled by the moderator in the calandria. With time, the moderator is evaporated and after some time, a hot dry debris bed is formed. The debris bed transfers heat to the calandria vault water which acts as the ultimate heat sink. However, the questions remain: how long would the vault water be an ultimate heat sink, and what would be the failure mode of the calandria vessel if the heat sink capability of the reactor vault water is lost? In the present study, a numerical analysis is performed to evaluate the thermal loads and the stresses in the calandria vessel following the above accident scenario. The heat transfer from the molten corium pool to the surrounding is assumed to be by a combination of radiation, conduction, and convection from the calandria vessel wall to the vault water. From the temperature distribution in the vessel wall, the transient thermal loads have been evaluated. The strain rate and the vessel failure have been evaluated for the above scenario.

Effect of stress relief heat treatment on the residual stress and hardness of additively manufactured Ti-6Al-4V alloy (응력제거 열처리 공정조건이 적층제조한 Ti-6Al-4V 합금의 잔류응력 및 경도에 미치는 영향)

  • Yeonghwan Song
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.33 no.6
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    • pp.282-287
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    • 2023
  • The effect of stress relief heat treatment temperature and duration time on the microstructure, residual stress and Vickers hardness of additively manufactured Ti-6Al-4V alloy using laser powder bed fusion process was clarified. As a result of stress relief heat treatment for 240 minutes at 823 K and 60 minutes or more at 873 K, residual stress was decreased less than 30 MPa without grain growth and phase transformation which causes dimensional distortion and deterioration of mechanical properties. In addition, hardness was increased with increasing heat treatment temperature and duration time. It was deduced that the refinement of acicular martensitic α' phase due to the increasing duration time of isothermal heat treatment at 773~873 K, which was not detected by XRD and phase map analysis using SEM-EBSD, probably increases the hardness.

Effect of Mixed Bed Soil on Growth of Aerial Parts and Roots of Daughter Pants for Nursery Field Srawberry Seedling Raising with Expanded Rice-hull (딸기 팽연왕겨 차근육묘를 위한 혼합상토 조성이 자묘의 지상부 생육과 발근에 미치는 영향)

  • Park, Gab-Soon;Kim, Young-Chil;Kim, Myung-Seon;Ann, Seoung-Won
    • Journal of Environmental Science International
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    • v.24 no.2
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    • pp.189-196
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    • 2015
  • This study was performed to identify the effect of mixed bed soil on growth of aerial parts and root zone of daughter plants for nursery field strawberry seedling raising with expanded chaff. The plant height and leaf area of daughter plants were highest or largest in the mixed soil of ERH +RH (100:0, v/v), followed by ERH+RH (75:25). The higher the mixing ratio of RH, the shorter the plant height or the smaller the leaf area. A similar tendency was observed in fresh weight. Within a root diameter of 0-0.4 mm and a root height range of 0.4-0.8 mm, root surface area and volume were statistically significantly better with treatment of ERH+RH (100:0, v/v) compared to those of roots treated with ERH+RH (75:25), ERH+RH (50:50) and ERH+RH (25:75). The growth rate of aerial parts and root zone of daughter plants were noticeably lower in two mixing ratios of 50:50 and 25:75. According to the mixing ratios of ERH+CD surface treatment, the number of roots was greatest in plants treated with ERH+CD (80:20, v/v) and ERH+CD (85:15) on August 1. However, the number of roots was highest in plants treated with ERH+CD (85:15, v/v) on August 15. Root length was longest in the plant with no treatment, and drastically shortened from ERH+CD (90:10, v/v) in both surface and mixed treatment. Although root weight showed a significant difference in ERH+CD (90:10, v/v) treatment, its increase was gradual. The rate of root growth was highest in ERH+CD (85:15). These study findings suggest that the content ratios of mixed soil ERH+RH (75:25, v/v) or below and ERH+CD (85:15) are thought to be desirable for the production of high quality seedlings.

활성탄에 의한 염소가스의 흡착특성

  • 이재의;윤세훈;김동현;김준형;오원진
    • Proceedings of the Korean Nuclear Society Conference
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    • 1998.05b
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    • pp.397-402
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    • 1998
  • 염소가스의 활성탄 흡착성능을 흡착평형과 flow system 두 단계의 흡착실험을 통하여 측정하였다. 활성탄은 surface area와 pore size distribution이 구별되는 세가지 이상의 시료를 선정하였고 활성탄의 표면특성에 따른 염소가스의 흡착관계를 검토하였다. Flow system에서는 염소가스를 500ppm의 농도(v/v in helium) 를 가지는 염소가스를 선택하였고 GC로 흡착경향을 분석하였다. 주어진 흡착탑(long bed)에서 흡착용량의 증가변화에 대한 흡착속도의 감소변화의 추이를 관찰하였으며 이로부터 염소가스 단일성분의 활성탄 흡착탑에 대한 흡착모델의 설계가 가능함을 알 수 있었다.

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Continuous Production of Isomaltooligosaccharides by Immobilized Transglucosidase in a Packed-bed Reactor (충진형반응기에서 고정화 Transglucosidase를 이용한 이소말토올리고당의 연속생산)

  • Ahn, Jang-Woo;Park, Kwan-Wha;Seo, Jin-Ho
    • Korean Journal of Food Science and Technology
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    • v.30 no.1
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    • pp.110-117
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    • 1998
  • A packed-bed reactor with immobilized transglucosidase (TG) was operated to test the possibility of continuous production of isomaltooligosaccharides (IMO) and the effect of concentration and feed rate of substrate solution on the production pattern as well as operational stability The pattern of formation of IMO was the same to the one of soluble TG. The concentrations of glucose and isomaltose produced by the packed-bed reactor were gradually decreased as the flow rates were increased regardless of the concentrations and kinds of maltose solution as substrate. Isomaltotriose showed the same tendency except 10% maltose solution. But the concentration of panose was increased and then decreased as the flow rates were increased. The maximum yield of IMO was 52.1% when 10% (w/v) solution was fed to the reactor at 2 mL./min feed rate. When each 20% and 30% (w/v) solution was respectively used at $0.5{\sim}1.0\;mL/min$, the maximum yield were $39.0{\sim}38.0%\;and\;12.1{\sim}14.2%$. The maximum yield was 36.3% at $0.5{\sim}1.0\;mL/min$ when a commercial maltose product containing 20% maltose was used. The reactor was stably operated at $55^{\circ}C$. 85% and 65% of initial activity was maintained for 144 hours and 288 hours of operation, respectively. A reactor analysis strongly an immobilized TG system could apply to continuous production of IMO.

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Continuous Nitrate Removal using Bipolar ZVI Packed Bed Electrolytic Cell (영가철(Fe0) 충진 복극전해조를 이용한 질산성질소의 연속식 제거 연구)

  • Jeong, Joo-Young;Kim, Han-Ki;Shin, Ja-Won;Park, Joo-Yang
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.1B
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    • pp.79-84
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    • 2012
  • Nitrate is a common contaminant in groundwater aquifer. The present study investigates the performance of the bipolar zero valent iron (ZVI, $Fe^0$) packed bed electrolytic cell in removing nitrate in different operating conditions. The packing mixture consists of ZVI as electronically conducting material and silica sand as non-conducting material between main cathode and anode electrodes. In the continuous experiments for the simulated wastewater (contaminated groundwater, initial nitrate about 30 mg/L as N and electrical conductivity about 300 ${\mu}S/cm$), over 99% removal of nitrate was achieved in the applied voltage 600 V and at the flow rate of 20 mL/min. The optimum packing ratio (v/v) and flow rate were determined to be 1:1~2:1 (silica sand to ZVI), 30 mL/ min respectively. Effluent pH was proportional to nitrate influx concentration, and ammonia which is the final product of nitrate reduction was about 60% of nitrate influx. Magnetite was observed on the surface of the used ZVI as major oxidation product.

Effects of Base Metal on the Partial Oxidation of Methane Reaction (메탄의 부분산화반응에 미치는 Base metal의 영향)

  • 오영삼;장보혁;백영순;이재의;목영일
    • Journal of Energy Engineering
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    • v.8 no.2
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    • pp.256-264
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    • 1999
  • The performance of the Pt-B/cordierite catalysts (2 wt%) Pt, 70 wt% Alumina, 28 wt%) Ceria and Zirconia, B: base metal) loaded with 6∼12 wt% Mn, Cu, V, Co, Cr and Ba, respectively was studied for partial oxidation of methane reaction and compared with that of Ni loaded catalyst. As a results, it was found that Ba, Co, Cr as well as Ni loaded catalysts showed higher activity for methane partial oxidation of methane than the Mn, Cu and V loaded catalyst. But it was known that catalysts having good activity for methane showed the good activity for coke formation, too. A XRD analysis of the catalyst before and after the reaction using 5 wt% Ni/Al$_2$O$_3$) showed that there were three Ni phases. In these results, it was found that methane oxidation reaction occulted at the front of the catalyst bed consisted of NiAl$_2$O$_4$and NiO and reforming reaction occurred at the rear part of the catalyst bed consisted of reduced Ni.

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Establishment of artifical cultivation technique of Pleurotus ostreatus using an onion net (양파망을 이용한 느타리버섯(Pleurotus ostreatus) 재배기술)

  • Yu, Young-Jin;Seo, Sang-Young;Jung, Gi-Tai;Ryu, Jeong;Ko, Bok-Rai;Choi, Joung-Sik;Kim, Myung-Kon
    • Journal of Mushroom
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    • v.4 no.3
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    • pp.101-105
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    • 2006
  • This was performed to improve the working conditions of oyster mushroom cultivation on bed using rice straw of which binding and cutting procedure need a lot of time. There was a little difference in physiochemical components between rice straw and cotton waste C/N ration of cotten waste was, however, similar to rice straw as 84-85. If is feasible to use as substrate material. The average yield of a net was 1450g in six frustration harvest, when the size of an onion net was $35{\times}30cm$, mixture ratio of rice straw to cotton waste was 40:60(V/V). Cultivation method using an onion net saved 3 days for preparation, 4 hours for inoculation compared to normal bed cultivation. This method was analysed to give 5% economical benefit.

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Changes of Physicochemical Parameters During the Aerobic Composting Process of Swine Manure (돈부의 호기성 퇴비화 단계별 물리.화학적 성상 변화)

  • 김태일;정광화;최기춘;류병희;곽정훈;전병수;박치호;김형호;한정대
    • Journal of Animal Environmental Science
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    • v.3 no.1
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    • pp.13-18
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    • 1997
  • This study was conducted to investigate the physicochemical changes during the aerobic composting of swine manure mixed with bulking agent, sawdust(v/v, 1:1), in a full-scale composting plant using rectangular escalator-aginated bed composting system. Physical and chemical properties were analyzed on the samples which were collected at 5, 15, and 25 day of composting, curing and final step. The results of this study were summarized as follows; 1. Moisture and K2O content, and pH of final step were higher than those of 5th day of composting (p<0.05). 2. Ammonium nitrogen, total organic corbon and organic matter content, and electrical conductivity(EC) were significantly decreased (p<0.05) but nitrate nitrogen, ash and P2O5 content increased(p<0.05) throughout the aerobic composting process. 3. Total organic carbon per total nitrogen(C/N) and total organic matter per total nitrogen(OM/N) ratio were significantly decreased throughout the aerobic composting process(p<0.05). 4. Physical and chemical properties of swine manure were varied by aerobic fermentation using rectangular escalor-aginated bed composting system.