• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.891-900
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• 2004

UDP-N-Acetylglucosamine(GlcNAc):$\beta$1,4-D-mannoside$\beta$-l ,4N-acetylglucosaminyltransferase-III (GnT-III) and UDP-N-GlcNAc:$\alpha$-6-D-mannosid$\beta$-1,6N-acetylglucosaminyltransferase-V(GnT - V) activities were determined in human hepatoma cell lines and metastatic colon cancer cells, and their activities were compared with those of normal liver cells and fetal hepatocytes. GnT-III activities were higher than those of GnT-V in hepatic carcinoma cells. When the two enzyme activities were assayed in highly metastatic colon cancer cells, GnT - V activities were much higher than those of GnT-III. When GlcN, GlcN-biant-PA and UDP-GlcNAc were used as substrates, the enzymes displayed different kinetic properties between hepatic and colon cancer cells, depending on their metastatic potentials. Normal cells of two origins had characteristically very low levels of GnT-III and -V activities, whereas hepatoma and colon cancer cells contained high levels of activities. These data were supported by RT-PCR and Northern blot analyses, showing that the expression of GnT-III and -V mRNAs were increased in proportion to the enzymatic activities. The increased GnT-III, md -V activities were also correlated with increased glycosylation of the cellular glycoproteins in hepatoma and colon cancer cells, as examined by lectin blotting analysis by using wheat germ glutinin (WGA), erythroagglutinating phytohemagglutinin (E-PHA), leukoagglutinating phytohemagglutinin (L-PHA), and concanavalin A (Con A). Treatment with retinoic acid, a differentiation agent, resulted in decreases of both GnT-III and -V activities of HepG2 and HepG3 cells. In colon carcinoma cells, however, treatment with retinoic acid resulted in a reduction of GnT-V activity, but not with GnT-III activity. Although the mechanism underlying the induction of these mzymes is unclear, oligosaccharides in many glycoproteins have been observed of cancer cells.

• Proceedings of the Korean Society for Quality Management Conference
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• 2004.04a
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• pp.114-119
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• 2004

Software verification and validation(V&V) is a means to develop high-quality software and assure safety and reliability for software. Also, we can achieve the desired software quality through systematic V&V activities. The software to be applied safety critical system like nuclear power plants is required to setup the V&V methodology that comply with licensing requirements for nuclear power plants and should be performed V&V activities according to it. In this paper, we classified safety-critical, safety-related and non-safety for software according to safety function to be peformed and define V&V activities to be applied software grade. Also, we defined V&V activities, procedures and documentation for each phase of software development life cycle and showed techniques and management to perform V&V. Finally, we propose the V&V framework to be applied software development of SMART(System-integrated Modular Advanced ReacTor) MMIS (Man-Machine Interface System) and to comply with domestic licensing requirements.

• Journal of the Ergonomics Society of Korea
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• v.32 no.5
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• pp.437-442
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• 2013

Objective: The objective of this study was to investigate the effects of drive-assisting system in a shower carrier on the upper body muscle activities of caregivers through drivability tests. Background: In care facilities, one of the major ADL (Activities of Daily Living) factors is bathing/showering. Recently, bath/shower-assisting equipment is actively being introduced in care facilities to reduce caregivers' muscle burden. In particular, it is desirable to utilize a shower carrier equipped with drive-assisting system to effectively care for the elderly. However, there were few systematic studies on the relationship between muscle activities and drive-assisting speeds. Method: For the drivability tests to study the effects on the muscle activities according to the drive-assisting speeds(corresponding drive-voltages: 0.0V, 2.0V, 2.1V, 2.3V), 6 females in their 40s($43{\pm}4yrs$, $157{\pm}5cm$, and $54.5{\pm}1.5kg$) were selected. To measure muscle activities of caregivers through drivability tests, 7 muscles in the upper body(TM/Trapezius Muscle, DM/Deltoid Muscle, BBM/Biceps Brachii Muscle, TBM/Triceps Brachii Muscle, ECRLM/Extensor Carpi Radialis Longus Muscle, FCUM/Flexor Carpi Ulnaris Muscle, and ESM/Erector Spinae Muscle) were selected. Results: In the TM, muscle activities were decreased as 21% compared to 0.0V, when drive-voltage 2.0V was applied, as 57% by 2.1V, and 62% by 2.3V(p<0.05), whereas 40%, 56%, and 69% of muscles activities were decreased respectively from the DM(p<0.05). Also, from the UL(BBM+TBM+ECRLM+FCUM), muscle activities were decreased by 17% with 2.0V as against 0.0V, by 47% with 2.1V, and 52% with 2.3V, whereas decreases in muscle activities from the ESM were found by 20%, 34%, and 42% respectively by 2.0V, 2.1V, and 2.3V(p<0.05). Conclusion: The muscle activities were decreased in the order of the DM, TM, ESM, and UL. As muscle activities were remarkably reduced as drive voltage were increased, it was expected to reduce the upper body muscle burden on the caregivers when using shower carriers equipped with driving-assist system. Applications: The results from this study can be applied for the development of a shower carrier including other equipment to possibly reduce the muscle burden of the caregivers.

• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.330-336
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• 2004

Fifty-two pathogenic Vibrio parahaemolyticus strains were isolated from the environments of Busan and Yeosu, Korea. Forty-three of these strains showed protease activities, whereas 4 strains showed $\alpha / \beta$ hemolysin activities and 6 strains had urease activities. Their pathogenic factors were not overlapping except one strain, which had both protease and hemolysin activities. The 6 urease-positive strains (V. parahaemolyticus YKB4, YKB14, S25, YFB20, YFO21, and YFO22) showed the same biochemical characteristics as a reference strain [V. parahaemolyticus KCTC 2471 (urease-negative)], except for urease production. The 6 urease-positive strains showed different urease activities in their culture supernatant during the growth. The urease activity of S25 increased sharply at the late exponential phase, and was the highest at the initial stationary phase and was kept until the late stationary phase. The other 5 isolates, except C25, showed urease activities at the mid-stationary phase and increased steadily until the late stationary phase, when the urease activity was maximal. To compare the degree of virulence of V. parahaemolyticus with different pathogenic factors, hemolysin, protease, or urease-positive strains were injected into groups of 10 each of ICR mice (7- to l0-week-old males). The lethal rates of urease-positive V. parahaemolyticus, YKB14, YKB4, and S25, were significantly high, being 50, 70, and 80%, respectively. Protease-positive V. parahaemolyticus strains FM39 and FM50 showed 40% and 60% of lethal rate, respectively. Hemolysin-positive V. parahaemolyticus strains S34 and S72 had no mortality, similar to nonpathogenic V. parahaemolyticus FM12.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.9
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• pp.397-404
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• 2019

When developing a weapon system, a T&E(Test and Evaluation) can be performed using M&S for the test items that cannot be evaluated in the real world. In this case, the VV&A activities are required to prove the credibility of M&S for the T&E. Recently, the use of M&S has been increasing as the R&D trends of weapon systems are becoming more advanced. Therefore, the VV&A activities are also increasing. The VV&A activities aim to verify, validate, and accredit that the simulation can represent a real system and ensure credibility regarding its purpose and intention of use. VV&A activities are divided into V&V and Accreditation. When performing VV&A in the ADD (Agency for Defense Development), the V&V activities are performed by a separate department of the ADD and the accreditation activities are performed in the DTAQ (Defense Agency for Technology and Quality). This paper proposes a V&V process for a T&E of M&S that has been performed in ADD. The process is used to verify and validate the documents and data generated during the development process according to the accreditation criteria, and provides objective data that can be used to judge whether the accreditation decision and acceptance criteria are met.

• Proceedings of the KIEE Conference
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• 2005.05a
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• pp.185-187
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• 2005

The reactor protection system is the most important function for the safe operation of nuclear powerplants (NPPs) in that such system protects a nuclear reactor tore whose damage can cause an enormous disaster to the nuclear facility and the public. A digital reactor protection system (DRPS) is being developed in KAERI for use in the newly-constructed NPPs and also for replacing the existing analog-type reactor Protection systems. In this paper, an software verification and validation (V&V) activities for DRPS, which are independent of the DRPS development processes, are described according to the software development life cycle. The main activities of DRPS V&V processes are the software planning documentations, the verification of software requirements specification (SRS) and software design specification (SDS), the verification of codes, the tests of the integrated software and system. Moreover, the software safety analysis and the software configuration management are involved in the DRPS V&V processes. All of the V&V activities are described, in detail, in this paper.

• Journal of Food Hygiene and Safety
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• v.15 no.2
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• pp.173-175
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• 2000

Nine strains of pathogenic Vibrio sp. of clinical and environmental origin were examined for the degradation of gelatin, casein and hemolysin which is important to the virulence of this bacterium. Culture filtrates of all nine strains of Vibrio exhibited proteolytic activities. Especially, four strains of V. parahaemolyticus and one V. alginolyticus showed strong gelatin-degrading activity. In terms of hemolytic activity, three V. parahaemolyticus and V. mimicus showed strong $\beta$-hemolysis whereas those of strains of V. alginolyticus, V. fluvialis, V. vulnificus examined lacked this activity.

• Fisheries and Aquatic Sciences
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• v.7 no.1
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• pp.10-15
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• 2004

A total of 52 pathogenic Vibrio strains was isolated from the Gwangan Beach during summer in 2003. The isolated vibrios were composed of 6 different species: V. parahaemolyticus, V. cholerae non O1, V. fluvialis, V. vulnificus, V. alginolyticus, and V. mimicus. V. parahaemolyticus was most predominant as $46\%$ (24/52), V. cholerae non O1 was the second with $23\%$ (12/52), and V. fluvialis was the third with $17\%$ (9/52). Among the isolated strains, 22 strains showed hemolytic, proteolytic or ureolytic activity. Eight strains showed both hemolysin and protease activities, and either 6 strains showed only hemolysin activities and 7 strains only protease activities. Only one strain of V. parahaemolyticus isolates showed urease activity. The urease-positive V. parahaemolyticus strain (V. parahaemolyticus S25) showed the same biochemical characteristics as the reference strain, V. parahaemolyticus KCTC 2471 (urease­negative) except for urease production. To compare the degree of virulence of Vibrio strains having different pathogenic factors, hemolysin, protease, or urease-positive strains were injected into groups of 10 each of ICR mice (7- to l0-week-old male). The lethal rate of urease-positive V. parahaemolyticus S25 was significantly high, being $70\%$. Protease-positive strains showed $40-60\%$ of lethal rate. Hemolysin-positive strains showed no mortality, similar to non-pathogenic V. parahaemolyticus KCTC 2471 and V. parahaemolyticus FM12.

• Journal of KIISE:Computing Practices and Letters
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• v.15 no.6
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• pp.435-439
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• 2009

Software used in safety-critical system must have high dependability. Software testing and V&V (Verification and Validation) activities are very important for assuring high software quality. If we can predict the risky modules of safety-critical software, we can focus testing activities and regulation activities more efficiently such as resource distribution. In this paper, we classified the estimated risk class which can be used for deep testing and V&V. We predicted the risk class for each module using support vector machines. We can consider that the modules classified to risk class 5 and 4 are more risky than others relatively. For all classification error rates, we expect that the results can be useful and practical for software testing, V&V, and activities for regulatory reviews.

• Bulletin of the Korean Chemical Society
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• v.28 no.12
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• pp.2459-2465
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• 2007

V2O5/TiO2-ZrO2 catalyst modified with V2O5 was prepared by adding Ti(OH)4-Zr(OH)4 powder into an aqueous solution of ammonium metavanadate followed by drying and calcining at high temperatures. The characterization of prepared catalysts was performed using XRD, DSC, solid-state 51V NMR, and FTIR. In the case of calcination temperature of 500 oC, for the catalysts containing low loading V2O5 below 25 wt % vanadium oxide was in a highly dispersed state, while for catalysts containing high loading V2O5 equal to or above 25 wt % vanadium oxide was well crystallized due to the V2O5 loading exceeding the formation of monolayer on the surface of TiO2-ZrO2. The strong acid sites were formed through the bonding between dispersed V2O5 and TiO2-ZrO2. The larger the dispersed V2O5 amount, the higher both the acidity and catalytic activities for acid catalysis.