• Radiation Oncology Journal
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• 제21권2호
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• pp.135-142
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• 2003

목적: 자궁경부암으로 진단 받고 근치적 방사선치료를 받은 환자에서 방사선 치료 전과 치료 중 자기공명영상을 비교함으로써 삼차원입체조형치료나 강도변조방사선치료의 효과에 영향을 미칠 수 있는 자궁의 크기 및 위치 변화를 알아보았다. 대상 및 방법: 1997부터 2000년까지 삼성서울병원에서 자궁경부암으로 근치적 방사선치료를 시행 받은 환자 중 방사선치료 전과 치료 중간에 자기공명영상 촬영을 시행한 47명의 환자를 대상하였다. 방사선치료는 전골반조사와 강내조사가 시행되었는데 전골반조사는 골반 전체에 통상적 4문 조사법으로 매일 1.8 Gy씩, 주 5회 실시하며 총선량 50.4$\~$55.8 Gy (30.6$\~$45 Gy 이후에 골반중심부차폐) 조사하였고, 강내조사는 A점에 4 Gy씩, 매주 2회 시행하여 총선량 24 Gy까지 시행하였다. 자기공명영상 촬영은 방사선치료 시작 전과 치료 시작 후 3$\~$4주경 강내조사와 골반중심차폐를 하기 전에 실시되었다. 방사선치료 전과 치료 중 촬영된 자기공명영상의 T2 강조 시상면 영상과 축면 영상에서 자궁의 변화를 알기 위하여 자궁경관 크기, 자궁 크기, 자궁체부 크기, 자궁경관 굴곡각, 자궁체부 굴곡각, 과 자궁체부 회전각을 측정하였다. 결과: 자궁경관 크기는 치료 전에 평균 36.7 mm에서 치료 후 27.8 mm로 감소하였고, 자궁체부 크기는 평균 2.5 mm 감소하였으며, 자궁크기 차이는 평균 6.4 mm였다. 자궁경부에서 자궁저부까지의 거리 차이는 평균 15.2 mm였으며, 이 중 6예에선 30 mm 이상 크기가 줄었는데 이 경우에 종양의 크기가 모두 4 cm 이상이었다. 자궁경관 굴곡각은 평균 13.2$^{\circ}$, 자궁체부 굴곡각은 평균 16.9$^{\circ}$ 자궁체부 회전각은 평균 13.1$^{\circ}$ 차이가 있었다. 회전이나 굴곡 중 어느 하나라도 30$^{\circ}$이상 변화된 경우가 전체 환자 중 9예(19.1$\%$)가 있었다. 연령과 자궁경관 굴곡각의 차이는 통계적으로 유구한 연관이 있었고, 종양 크기에 따라 자궁경관 크기, 자궁 크기, 자궁체부 크기와 자궁경관 굴곡각은 모두 유의한 변화가 있었다. 그리고, 종양 크기가 4 cm 이상인 경우에 자궁경관의 크기, 자궁 크기와 자궁체부의 크기 변화가 모두 통계적으로 의미있는 차이를 보였다. 또한, 자궁경부로부터 자궁저부까지 거리 차이는 종양 크기가 4 cm 미만인 경우는 5.3 mm였으나 4 cm 이상일 때는 19.4 mm로 현저한 변화를 보였다. 자궁경관 굴곡각은 60세 미만인 경우 60세 이상보다 8$^{\circ}$ 정도 더 변화가 있었고, 종양 크기가 4 cm 이상일 때 미만일 때보다 2배 이상 굴곡 변화가 있었다. 결론: 자궁경부암 환자에서 근치적 방사선치료 시 치료에 따른 자궁 크기 및 위치 변화가 다양하고 개별적으로 예측하기 쉽지 않으므로, 특히, 60세 미만이거나 종양 크기가 4 cm 이상인 경우, 삼차원입체조형치료나 강도변조 방사선치료를 이용한 근치적 방사선치료 시 치료 중 개별환자의 계획용표적체적의 변화를 반드시 고려해야 한다고 생각한다.

• 한국임상수의학회지
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• 제17권1호
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• pp.285-288
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• 2000

Nine immature 30-day-old female rats were injected sc at 0800 hr with pregnant mare serum gonadotrophin(PMSG) to induce ovulation and mating. Fifty-six hours later the animals were placed with mature male rats overnight (one female and one male). Five of 9 immature female rats treated with PMSG were pregnant and allowed to maintain the pregnancy to term. Three of 5 pregnant rats were failed to maintain pregnancy to term. Two of 5 pregnant rats seemed to be developed normally and increased abdominal enlargement as pregnancy progresses, but did not occurred parturition on day of 43 or 48 of pregnancy, respectively. On day 44 or 49, pregnant rats were killed and examined uterus and ovaries. There was no fetus but approximately 50∼60ml. of mucopurulent fluids were accumulated in the uterine cavity and 40 or 42 corpora lutea persisted in the ovaries. Pyometra was developed after coitus in PMSG-treated immature female rat.

• 한국동물위생학회지
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• 제30권3호
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• pp.429-436
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• 2007

This study was performed to make helpful standard for discrimination of cows from heifers by uterine and ovarian section inspection. A total of 3,736 heifers and 249 cows were inspected from January to November 2006 in Karak Market Slaughter house. Firstly, it was obvious that non-corpus albicans were observed in almost heifers(3,597/3,736, 96.3%), but there were distinctive corpus albicans in all cows (249/249 100%). Secondly, another evident was that the length and diameter of the horn in uterus was below 30mm in 94.5% (3,267/3,736) heifers, but above 30mm in 90.4% (225/245) cows, and it was easy to distinguish between right and left horn by difference of size in 87.6% (218/249) cows. Thirdly the diameters of pelvic cavity was below 220mm in 96.8% (3,615/3,736) heifers, but was over 200mm in 85.1% (212/249) cows. Fourthly another difference was found in second sacrum union condition and 13th thoracic vertebral process's states. In case of heifers, it was distinctive five lines in each second sacrum, but it was non-distinctive or united in the same each lines of the sacrum in cows. Fifthly in the results of 13th thoracic vertebral colors, the ratio between red color and white color was 7 : 3 in heifers (3,385/3,736, 90.6%), but that was 2 : 8 in cows (199/249, 79.9%). Consequently, it was possible to use the method which discriminated cows from heifers by uterine and ovarian section inspection in large scaled field slaughter house.

• 한국발생생물학회지:발생과생식
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• 제21권2호
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• pp.151-156
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• 2017

High-fructose corn syrup (HFCS) is widely used as sweetener, and its overconsumption is become a major health problem. In the present study, we used adult female rats and applied a 28 days HFCS feeding model to monitor the estrous cycle and changes in tissue weights and histology. Adult female rats were divided into three groups. Animals were fed with ad libitum normal chow and (1) 24 hours tap water (Control group), (2) 12 hours HFCS access during dark period and 12 hours tap water (12H group), and (3) 24 hours HFCS only access (24H group). Total exposure period was 28 days. There is no significant change in body weight between control and HFCS-fed animals. Both absolute and relative weights of ovary in 24H animals were significantly heavier than those in control or 12H animals. The absolute and relative weights of the kidney and liver in 24H groups were significantly heavier than those in control or 12H animals. The estrous cycles of the 24H animals were significantly longer. Histological analyses revealed that 24H ovaries were relatively bigger and possessed more corpus lutea than control ovaries. Uterine sections of 12H and 24H animals showed a well-developed stratum vasculare between inner and outer myometrial layers. The number of endometrial glands were decreased in 12H uteri, and recovered in 24H uteri compared to control. Numbers of convoluted tubule in distal region increased in 12H and 24H kidney samples. Liver specimens of 12H and 24H showed the increased number of fat containing vacuoles. In conclusion, our study demonstrated that HFCS treatment for 28 days could induce (1) changes in length of estrous cycle with extended estrous and diestrous stages, (2) altered ovarian and uterine histology, and (3) liver and renal lipid accumulation. These findings reveal the adverse effects of HFCS drinking on the reproductive function and lipid metabolism of female rats.

• 대한수의학회지
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• 제40권4호
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• pp.769-775
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• 2000

This study was investigated the ovarian response following superovulation treatment at different day of diestrus. The criterion for the presence or absence of a dominant follicle based on their morphological examination. Dominant follicle was puntured 48 hrs before the oneset of superovulation treatment by ultrasonography guided aspiration needle. Superovulation was induced by subcutaneous administration of FSH twice a day for 4 day in a decreasing regimen. There was no significant different between presence of dominont follicle and progesterone concentration/diameter of corpus luteum in HanWoo. Number of corpus luteum of donor after superovulation treatment was not significantly different in FSH administration at day 9, 11 and day 13 of estrus($14.5{\pm}4.5$, $15.5{\pm}5.6$ and $11.0{\pm}5.5$, respectively). But, the diameter of CL was significantly correlate(R2 = 0.757) with progesterone levels on day of superovulatory induction. After 7 days of artificial insemination, the embryos at 7 days were collected by uterine flushing after dominant follicle aspiration and superovulation treatment, and evaluated their quality by morphological criteria. Fifty five embryos with excellent, good and fair grade were transferred into 24 recipient cows. Seventeen offsprings, 1 of triplet, 4 of twins and 6 of singlet, were yield from 10 recipient cow. In conclusion, the present study showed that 1) dominant follicle can be determined by ultrasonography with rectal palpation by morphological evaluations, 2) superovulation response after follicular aspiration was not differ at day 9, 11 and 13 of estrus, 3) dominant follicle did not affect to progesterone concentration and diameter of CL, and 4) diameter of CL was significantly correlate to the level of progesterone concentrations in HanWoo.

• 한국임상수의학회지
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• 제7권2호
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• pp.511-515
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• 1990

소에 있어서 효율적인 쌍자생산 방법을 검토하기 위하여 이분수정란의 이식, 저단위 FSH에 의한 다배란 유도후의 인공수정, 인공수정후의 수정란이식, 수정란 2개의 양측 및 단측 자각에의 이식 등의 방범을 적용하여 본 실험을 수행하였다. 각 실험군의 60일 수태율은 각각 34.8%, 65.0%(평균), 66.7%, 62.5%, 57.1%였으며 쌍태임신율은 8.7%, 15.0%(평균), 16.7%, 18.7%, 21.4%였다. 위의 결과에서 볼 때 저단위 FSH 투여법(Group B1)과 수정란 2개의 단측 자궁각 이식방법에 있어 쌍태율이 높게 나타나는 경향이 있었으나 실용적 측면에서는 낮은 수준이었다.

• Journal of Animal Science and Technology
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• 제64권1호
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• pp.1-9
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• 2022

Equilume light masks had no impact on hastening the resumption of estrous cyclicity in mares maintained in outdoor pastures on the mainland of Korea due to the cold weather conditions. Jeju Island is a major horse-breeding site in Korea and is warmer than the mainland during the winter season. Therefore, the primary objective of this study was to explore the efficiency of the Equilume light mask on the resumption of seasonal estrous cycles in Thoroughbred mares on Jeju Island. A total of 20 nonpregnant mares were randomly divided into the Equilume light mask (n = 9) and stable lighting (n =11) groups. The experiment was performed at seven different horse-breeding farms located on Jeju Island from November 15, 2020, to February 15, 2021. The mares were exposed to the respective lights from 16:00 to 23:00. Follicle size and uterine edema were measured by ultrasound scanning. Body condition scores (BCS) were also monitored during the experiment. Statistical analysis was conducted using the SAS and SPSS software, and p-values of < 0.05 were considered statistically significant. Two of the nine (22.2%) mares in the Equilume light mask group and three of the 11 (27.28%) mares in the stable lighting group were still cycling in December and January, which were considered as all-year-round cycling mares. On February 15, there was no difference between groups in the resumption of early seasonal estrus cycle, which was determined by follicles > 25 mm in addition to uterine edema. All mares in the Equilume light mask group and five of the eight mares (62.5%) in the stable lighting group had resumed cycling. Interestingly, six of the seven mares (87.5%) in the Equilume light mask and four of eight mares (50%) in the stable lighting group had already ovulated on February 15 (p > 0.05), as determined by the presence of a recent corpus luteum. No difference was observed in BCS and uterine edema between groups (p > 0.05). In conclusion, the Equilume light mask can be an effective approach to induce early seasonal estrus cycles of mares in Jeju Island, and it also enhances the efficiency of farm management by reducing labor.

• 한국수정란이식학회지
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• 제1권1호
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• pp.76-80
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• 1986

This study was carried out to investigate the inte actions between recipients and embryos to compare pregnancy rates in bovine embryo transfer, such as synchrony and stage of embryos, synchrony and quality of embryos, synchrony and side of uterine horn, and preservation time and stage of embryos. Fifty-two embryos were transferred by surgically to 42 Holstein heifers, 3 Holstein cows and 7 Korean native heifers from Feb., 1985 to June, 1986. The results were as follows: 1. In the trial of interactions between synchrony and embryo stage, recipients synchronired from- hours to + 12 hours in synchrony and embryos from morulac stage to advanced blastoctyst stages showed reason able pregnancy rate. 2. Excellant (A) and good (B) grade embryos showed good pregnancy rate, 81.8% and 73.3% respectively, but fair (C) grade embryos showed poor, 25% only at the same boundary of recipient synchrony. 3. More recipients had corpus luteum on the right ovary than the left, and also had better pregnancy rate (26/32 vs 13/20,81.3% vs 65%). 4. A good pregnancy rates (over 60%) were obtained with the embryo transferred 17hrs after flush at room temperature or field condition in culture medium.

• 한국수정란이식학회지
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• 제11권3호
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• pp.301-307
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• 1996

This study was carried out to produce twin calves by embryo transfer in Hanwoo and investigate the pregnancy and twin rate by recipient's conditions. All recipients were bred at estrus by artificial insemination with Hanwoo semen and then transfered an additional embryo produced in vivo or in vitro to tbe uterine horn contralateral to the corpus luteum on Day 7. The results obtained were as follows ; 1. The pregnancy rate was higher in young recipients of 3 years (68.8%) than in old ones of 10 years and greater(36.4%). And for CL size pregnancy rate was 57.9, 45.4 and 60.1% in large, medium and small size of CL of recipients, respectively. 2. 447recipients were transferred an additional embryos at 7th day after Al and average pregnancy rate was 57.5% and twin production rate was 22.2%. 3. Average pregnancy and twin production rate by direct transfer methods of frozenthawed IVF embryos was 56.0 and 16.7%. 4. The ratio of male to female twin in a total of 55 twin pairs was 54.6%, and average gestation lengths of male to female and female to female twin were 280.6$\pm$5.4 and 279.715.4 days, respectively. Average birth weight of twins was beavior in male and male twin(23.2i5.8kg) than in male and female twin(20.5$\pm$2.6kg).

• 대한수의학회지
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• 제47권1호
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• pp.135-138
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• 2007

A 3 year-old female Afghan hound came to the Veterinary Referral Hospital of the College of Veterinary Medicine, Seoul National University for artificial insemination (AI) with frozen semen. In order to inseminate, semen was frozen in USA 3 years ago. Frozen semen was sent by air from Santiago to Seoul for AI. The stud died 2 years ago, so we could only use a limited amount of frozen semen in that estrus cycle. The number of total motile spermatozoa was $59.4{\times}10^6$and the total volume was 1.2 ml. The frozen spermatozoa were thawed in $70^{\circ}C$ water for 8 sec, which were then deposited at the bilateral uterine horns by a surgical method. The number of corpus luteum was 6. Sixty days after artificial insemination resulted in the birth of 4 puppies, all of which are alive and healthy.