• Journal of Korean Physical Therapy Science
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• v.15 no.2
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• pp.15-24
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• 2008

Background: The purpose of this study was to demonstrate the norepinephrine－induced nociceptive effects by monitoring catecholamines and its metabolites in human body. Methods: To exam the antisympathetic effect from the healthy volunteer(male:15, female:15) by monitoring changes of epinephrine, norepinephrine, dopamine, metanephrine, normetanephrine, and others of urine, a comparative study with warm water immersion ($40.8{\pm}0.3^{\circ}C$) and infrared (250W) was carried out. Results: The urinalysis showed that the concentration of epinephrine and norepinephrine were significantly decreased by both warm water immersion－and infrared－stimulated group of urine in 24 hours. Conclusion: Therefore, these results suggest that the diminished responsiveness on the epinephrine and norepinephrine to warm water immersion and infrared in volunteer may be, in part, related by the increased of antisympathetic effects.

• The Journal of Korean Medicine
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• v.17 no.1 s.31
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• pp.146-170
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• 1996

This study aimed to evaluate the anti-stress effects of Walgukhwan, Walgukhwan+Ejintang and Wal gukhwan+Samultang. The experimental animals treated with each extracts during 15 days before the immobilization stress. And each experimental animals were endowed with 12 hours immobilization stress. Then the brain, blood and urine catecholamine contents and serum total cholesterol, triglyceride and free fatty acid levels were studied. The result were as followed; 1. Walgukhwan, Walgukhwan+Ejintang and Walgukhwan+Samultang were not statistically effective to the changes of the body weight. 2. Walgukhwan was statistically effective to the increase of the blood norepinephrine content as compared with control. 3. Walgukhwan+Ejintang was statistically effective to the increase of the blood and urine norepinephrine contents as compared with control. 4. Walgukhwan+Samultang was statisticallv effective to the increase of the brain, blood and urine norepinephrine contents and also to the increase of the blood and urine dopamine contents as compared with control. 5. Walgukhwan was statistically effective to the increase of the serum free fatty acid level as compared with control. 6. Walgukhwan+Ejintang was statistically effective to the increase of the serum triglyceride and free fatty acid levels as compared with control. 7. Walgukhwan+Samultang was statistically effective to the increase of the serum total cholesterol, triglyceride and free fatty acid levels as compared with control.

• Korean Journal of Biological Psychiatry
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• v.5 no.2
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• pp.278-282
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• 1998

Various neurotransmitters have been proposed as possible mediators of penile erection. Especially, norepinephrine and serotonin might have a important role in sexual arousal and penile erection. And it could be hypothesized that the psychogenic impotence is associated with the depletion or imbalance of norepinephrine and serotonin from evidences, such as the symptomatic manifestation of depression and the antidepressantinduced sexual dysfunction. The authors investigates the association of norepienphrine and serotonin with psychogenic impotence. The psychogenic impotent group(PIG) consisted of twenty-three patients with psychogenic impotence and the controlled group(CG) consisted of twenty-seven patients without psychogenic impotence. PIG had no organic cause accounting for their erectile dysfunction. The Beck Depression Inventory(BDI) and the State-Trait Anxiety Inventory(STAI) were applied to each subject to assess mood, state anxiety(SA) and trait anxiety(TA). Plasma norepinephrine level from systemic blood and 5-hydroxyindoleacetic acid(HIAA) levels from 24-hours urine were measured in each subject. The mean score of BDI of PIG was significantly higher than that of CG(p=0.015). PIG had a tendency of higher TA compared with CG(p=0.054). And also SA was higher in PIG, but did not show significant difference(p=0.193). The level of norepinephrine was significantly lower in patient with psychogenic impotence(p=0.000). And the level of 24-hours urine 5-HIAA was lower in PIG but did not show significant difference(p=0.494). Although the authors did not exclude depressive disorders in PIG, the present findings suggest that psychogenic impotence might have higher depressive mood and trait anxiety, and be associated with the depletion of norepinephrine in systemic blood.

• Journal of Korean Academy of Nursing
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• v.29 no.2
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• pp.445-455
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• 1999

This study has been conducted on the nonequivalent control group pretest-posttest design in quasi experimental basis and newly born premature infants from intensive care unit of G Medical University Hospital in Inchon Metropolitan were selected in two groups of 21 infants each. The first group for experimental and the other for control. Data has been collected form October 30, 1997 to August 29, 1998. For the experimental group tactile and kinesthetic stimulation was applied 2 times a day for 10 days(10 : 00~11 : 00 hours in the morning and 17 : 00~18 : 00 in the afternoon). As a weight weighing instrument. electronic indicator scale(Cas Co. korea) was used. To determine urine cortisol concentration level in stress hormone, radio immune assay method was used. And high performance liquid chlomatography was used to determine urine norepinephrine, concentration level To determine behavior status, tools developed by Anderson et at(1990) and remodeled by Kim Hee-Sook(1996) were used. Collected data were analyzed with the SAS program using x$^2$-test, student t-test, repeated measures ANOVA and paired t -test. The result were as follow. 1. As for the daily weight gain. the experimental group showed first change in weight and this group also showed higher weight in the average weight than the control group. Statistically, however, there was no significant factor between the two group. 2. The cortisol concentration in urine showed decrease in the experimental group norepinephrine concentration in urine showed increase in both experimental and control groups. No statistical significance was shown between the two groups. 3. In the aspect of behavior status. the experimental group showed statistical significance by showing inactive in the state of alert and conversion to a positive state than the control group. In conclusion, the sensory stimulation in this study showed a positive aspect through there was no statistical significance in the weight gain and urine stress hormone concentration. In the behavior status, there was statistical significance in the frequency of staying inactive in the state of alert and conversion to a positive state.

• Journal of Oriental Neuropsychiatry
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• v.6 no.1
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• pp.51-59
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• 1995

This experiments were conducted to study anti-stress effects of Anjungtang on the contents of catecholamine in plasma and urine of rats stressed by immobilization. The main results, obtained were summerized as follow: 1 The contents of plasma and urine catecholamine increased significantly in the group of rats stressed by immobilization. 2 The contents of plasma catecholamine decreased with statistical significance in the group administered Anjungtang of rats stressed by immobilization compared with in the group administered non Anjungtang. 3.The contents of urine catecholamine decreased with in the group administered Anjungtang of rats stressed by immobilization compared with in the group administered non Anjungtang. Particulaly the content of urine norepinephrine decreased with statistical significance. 4. The weight of the body increased in the group administered Anjungtang of rats stressed immobilization compared with in the group administered non Anjungtang. According to the above results, this experiments concluded that Anjungtang had significant effecrs in reducing stress.

• Journal of Nutrition and Health
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• v.25 no.3
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• pp.248-255
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• 1992

This study was performed to investigate the effects of dietary protein content on recovery of laparotomized rats in terms of urinary epinephrine and norepinephrine plasma epinephrine and norepinephrine, . Fortyeigh male Sprague Dawley rats average-weighing 160g were divided into two diet groups ; LPD(10% low protein diet) group HPD(25% high protein diet) group After 3 days of adaptation period rats were given experimental diet for 14 days. Experimental period consists of 7-days pre-trauma and 7-days post-trauma period. Rats were laparotomized by 4cm abdominal incision under sodium pentobarbital anesthesia. During 7-days before and after the surgery urine and plasma were collected for the analysis of epinephrine and norepine-phrine. The results are as follows: 1) After laparotomy urinary epinephrine level was not increased in two diet groups. Urinary epinephrine concentration of rats in LPD group was considerably increased in post-trauma day 3 and thereafter gradually reduced. By post-trauma day 4 however urinary epinephrine concentration of rats in HPD groups was recovered to pre-trauma level 2) Urinary norepinephrine concentration of rats in LPD group was significantly increased after trauma(p<0.01) and the difference between LPD and HPD in post-trauma average norepinephrine concentration was not significant. 3) Post-traum average plasma epinephrine concentration was higher but not significant than that of pre-truma average in both groups [LPD(19.88ng/ml vs 20.93ng/ml) HPD(17.20ng/ml vs 19.37ng/ml)] 4) Plasma norepinephrine concentration of rats in LPD group was significantly increased in post-trauma period(p<0.01) In HPD group however post-trauma average plasma norepi-nephrine concentration was significantly lower than pre-trauma average. Thus the results suggest that norepinehrine concentration was affected by trauma and rats in HPD group excreted less amount of norepinephrine than rats in LPD group.

• Child Health Nursing Research
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• v.11 no.1
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• pp.125-131
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• 2005

Purpose: This study was done to evaluate the effects of massage on the level of stress hormone in the urine in preterm infants. Method: The design was a nonequivalent control group pretest-posttest design quasi experimental study. Fifty-eight preterm infants were assigned to the experimental(31) or control group(27). The data were collected from March 2002 to August 2003. The massage stimulation was provided to infants in the experimental group for 15-minutes twice a day for 10 days. On day 1 and day 10 of the study, a 24 hour-urine sample was collected for norepinephrine, epinephrine, and cortisol assays. In data analysis, SPSSWIN 10.0 program was utilized for descriptive statistics, ANOVA and t-test. Results: General characteristics of the two groups showed no significant differences, thus the two groups were found to be homogenous. The 24 hour-urine cortisol of the massage group (t=4.61, p=.000) was significantly reduced compared to the control group after 10 days. Conclusions: The results suggest that the massage stimulation can be used to reduce 24 hour-urine cortisol in preterm infants. Therefore, massage provided in the incubator is recommended for reduction of stress in preterm infants who are hospitalized in neonatal intensive care units.

• Journal of Veterinary Clinics
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• v.26 no.3
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• pp.268-272
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• 2009

An-11-year-old male Shih-tzu was admitted to emergency care unit of Haemaru Referral Animal Hospital with signs of dyspnea, anuria and depression. There were abnormalities on complete blood count and serum chemistry included leukocytosis with mild left shift, mild azotemia, and increased ALT activity. Fluid therapy(0.9% saline, 40 ml/hr) and antibiotics were immediately initiated. The patient began to vomit after 5 hours' rest and pale mucous membrane, bradycardia, and hypertension were noted. Abdominal ultrasonography revealed enlarged left adrenal gland and thrombus in caudal vena cava(CVC). Result of ACTH stimulation test was normal. Cytology of ultrasound-guided FNA smears showed numerous naked nuclei, which was suggestive of adrenal medullar tumor. Concentrations of 24 hour urine metanephrine and normetanephrine was moderately increased compared to those of a control dog. Adrenal mass was surgically removed, and biopsy of the CVC mass was obtained. After surgery the patient began to recover but the dog acutely developed cardiopulmonary arrest and died. On histopathology the adrenal mass and biopsy of the CVC mass were consistent with pheochromocytoma. On electron microscopic view norepinephrine specific granules were found in the cytoplasm of tumor cells.

• Journal of environmental and Sanitary engineering
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• v.5 no.2
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• pp.37-43
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• 1990

The term of stress was used by many scientists, but there were several difinitions about stress, and this term are using in societies frequently. Generally stress is defined as equilibrium, harmony, and adaptation to external stimulations, in 1930 s Hans Selye was the first scientist who used the term of stress and he pointed out the stress mean the function of force in body. When we get stress the stimulation go to the sympathetic-adrenomedulla system and epinephrine and norepinnephrine are secreted from adrenal medulla. Author tried to investigate the change of catecholamine secretion from rat urine which were exposed to 80 dB noise environment. The control rat urine released 0.03 $\pm$ 0.01 ng/ml of epinephrine and 0.18 $\pm$ 0.04 ng/ml, but in noise exposed group the epinephrine was 0.42 $\pm$ 0.07 ng/ml and norepinephrine was 2.16 $\pm$ 0.48 ng/ml.

• Biomolecules & Therapeutics
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• v.7 no.4
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• pp.322-328
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• 1999

In this study, it was tested whether the changes of catecholamines and its metabolites are related with the changes of blood pressure(BP) induced by different levels of lead exposure. Adult male SD rats were exposed to lead by giving drinking water containing 50(low doses), 200 and 1,000 ppm(high doses) of lead(as lead acetate) or sodium acetate(for control groups, supplying an identical amount of acetate) for 7 or 16 weeks. The systolic BP was measured in the unanesthetized state by the tail-cuff technique. Levels of catecholamines and its metabolites in urine were measured by HPLC-ECD. Rats receiving 200 and 1,000 ppm developed an elevation of systolic BP at 3 and 7 weeks compared with week 0, but blood pressure levels at 16 weeks returned to normal. For the 50 ppm lead treated group, systolic BP increased significantly at 7 weeks and 16 weeks. The concentrations of norepinephrine and VMA in the urine of lead exposed rats changed similarly to the changes of blood pressure, but blood viscosity levels in all lead treated rats increased continuously during all lead treatment periods. This result suggests that the changes of catecholamines and its metabolites in urine by lead intoxication may influence the changes of blood pressure.