• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.7 no.1
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• pp.49-59
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• 1997

This study was performed to analyze the purity of technical grade ${\alpha}$-naphthylamine, to establish optimal analytical condition of ${\alpha}$-naphthylamine in urine and to determine the urine sample of workers exposed to ${\alpha}$-naphthylamine. The purity of technical grade ${\alpha}$-naphthylamine were $96.5{\pm}2.38%$, $94.1{\pm}0.97%$, $97.0{\pm}0.02%$ by gas chromatography-mass selective detector. To analyze ${\alpha}$-naphthylamine in urine, high performance liquid chromatography-electrochemical detector and gas chromatography-electron capture detector operating conditions have been optimized by preliminary expriment. In high performance liquid chromatography-electrochemical detector, the mobile phase was consisted of acetonitrile(35%) and water(65%), and the flow rate was maintained at 1.0ml per minute. Optimal detective condition was 9.0V(10nA/V) of electrochemical detector. The recovery of sep-pak treatment method was highly estimated as pretreatment of ${\alpha}$-naphthylamine in urine. The free amine was isolated by gas chromatography-electron capture detector after basic hydrosis, sep-pak treatment, toluene elution and HFBA(heptafluoro-butyric anhydride) derivatization of urine. The recovery of ${\alpha}$-naphthylamine in urine was $98.73{\pm}3.29%$ by gas chromatography-electron capture detector. The sensitivity was more higher than that of high performance liquid chromatography-electrochemical detector. Urinary ${\alpha}$-naphthylamine was detected in only one worker among nine workers. The level of ${\alpha}$-naphthylamine in urine was 6.42 ng/ml.

• Clinical and Experimental Pediatrics
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• v.58 no.5
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• pp.183-189
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• 2015

Purpose: Catheter urine (CATH-U) and suprapubic aspiration (SPA) are reliable urine collection methods for confirming urinary tract infections (UTI) in infants. However, noninvasive and easily accessible collecting bag urine (CBU) is widely used, despite its high contamination rate. This study investigated the validity of CBU cultures for diagnosing UTIs, using CATH-U culture results as the gold standard. Methods: We retrospectively analyzed 210 infants, 2- to 24-month-old, who presented to a tertiary care hospital's pediatrics department between September 2008 and August 2013. We reviewed the results of CBU and CATH-U cultures from the same infants. Results: CBU results, relative to CATH-U culture results (${\geq}10^4$ colony-forming units [CFU]/mL) were widely variable, ranging from no growth to ${\geq}10^5CFU/mL$. A CBU cutoff value of ${\geq}10^5CFU/mL$ resulted in false-positive and false-negative rates of 18% and 24%, respectively. The probability of a UTI increased when the CBU bacterial count was ${\geq}10^5/mL$ for all infants, both uncircumcised male infants and female infants (likelihood ratios [LRs], 4.16, 4.11, and 4.11, respectively). UTIs could not be excluded for female infants with a CBU bacterial density of $10^4-10^5$ (LR, 1.40). The LRs for predicting UTIs based on a positive dipstick test and a positive urinalysis were 4.19 and 3.11, respectively. Conclusion: The validity of obtaining urine sample from a sterile bag remains questionable. Inconclusive culture results from CBU should be confirmed with a more reliable method.

• Journal of Environmental Science International
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• v.21 no.11
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• pp.1333-1338
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• 2012

In order to measure the minute amount of Cu(II) in our environment, cyclic voltammetry (CV) and square-wave stripping voltammetry (SWSV) were performed for a trace copper assay using bismuth immobilized on a carbon nanotube paste electrode. An analytical working range of 30 to $240{\mu}g/L$ Cu(II) was obtained for CV and SWSV. The SWSV precision obtained was 0.47 % (n = 15) RSD in $30.0{\mu}g/L$ Cu(II). The detection limit obtained was 3.1 ng/L Cu(II) using SWSV, while the CV yielded the nano-range detection limit through the pre-concentration step. By using this research method, Cu(II) value could be determined in the urine of human sample and in the brain of fish sample. This research can be effectively applied to other cases of measuring minute amount of Cu(II) in living organisms.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.277.1-277.1
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• 2003

A high-performance liquid chromatographic method using the liquid extraction procedure was developed for the determination of L -FMAUS. a new L -FMAU derivative, in rat plasma and urine using 3-aminophenyl sulfone as an internal standard. A 100-${\mu}\ell$ aliquot of distilled water containing the L -cysteine (100 mg/$m\ell$) was added to a 100-${\mu}\ell$ aliquot of biological sample. L-Cysteine was employed to protect binding between 5'-thiol of l and protein in the biological sample. (omitted)

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2015.10a
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• pp.285-286
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• 2015

• Journal of Sasang Constitutional Medicine
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• v.2 no.1
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• pp.167-178
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• 1990

In order to investigate experimentally the clinical effect of Soyangin-Hyongbangdojoksan (少陽人荊防導赤散) that was prescribed to cure the Bisuhanpyohanbyong (脾受寒表寒病) of Soyangin (少陽人). The author experimented various activities of dried extract from hyongbangdojoksan (Sample-I) and mixed extract of each dried extract of hyongbangdojoksan (Sample-II) by the methods prescribed in the experimental parts. The results summarized as follows. 1. In the acetic acid method experiment, analgesic effect was noted in sample-I & Sample-II. 2. Anti-inflammatory effect on the edema induced by carrageenin was noted in Sample-I. 3. Antipyretic effect is not noted in Sample-I and Sample-II. 4. On urinary volume change and blood electrolyte clearance, the result is not significant, on the contrary the urine electrolyte discharge is effective in Sample-I and Sample-II. According to the above results, the effects based on oriental medical references approximate to the actual experimental results.

• Bulletin of the Korean Chemical Society
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• v.33 no.7
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• pp.2156-2162
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• 2012

Many literatures focus on the biological relevance and the identification of biomarkers for disease activity assessment while less attention has been paid to the development of standard procedures for sample preparation and storage based on liquid chromatography technique. The influencing factors including protein precipitation, storage temperature, storage time, and reconstitution by ultra pure water were analyzed employing HPLC-DAD. The effects were investigated from five participants over three months by principal components analysis (PCA) and the values of percent changes (PC). The samples with protein precipitation might slow the rate of bacterial enzymatic conversion. After protein precipitation, the average PC of urine samples ($0.136{\pm}0.013$, n = 5) is relatively less than that of the serum samples ($0.173{\pm}0.026$, n = 5) for three months. Minimal effects on metabolic profiles of serum and urine (PC < 0.15) are reasonable for metabolomic studies after protein precipitation and storage at $-20^{\circ}C$ for two months.

• Journal of Preventive Medicine and Public Health
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• v.39 no.2
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• pp.165-170
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• 2006

Objectives : The determination of oxalate in urine is required for the diagnosis and treatment of primary hyperoxaluria, idiopathic stone disease and various intestinal diseases. We examined the possibility of using Fourier transform near-infrared (FT-NIR) spectroscopy analysis to quantitate urinary oxalate. The practical advantages of this method include ease of the sample preparation and operation technique, the absence of sample pre-treatments, rapid determination and noninvasiveness. Methods : The range of oxalate concentration in standard urine solutions was $0-221mg/{\ell}$. These 80 different samples were scanned in the region of 780-1,300 nm with a 0.5 nm data interval by a Spectrum One NTS FT-NIR spectrometer. PCR, PLSR and MLR regression models were used to calculate and evaluate the calibration equation. Results : The PCR and PLSR calibration models were obtained from the spectral data and they are exactly same. The standard error of estimation (SEE) and the % variance were $10.34mg/{\ell}$ and 97.86%, respectively. After full cross validation of this model, the standard error of estimation was $5,287mg/{\ell}$, which was much smaller than that of the pre-validation. Furthermore, the MCC (multiple correlation coefficient) was 0.998, which was compatible with the 0.923 or 0.999 obtained from the previous enzymatic methods. Conclusions : These results showed that FT-NIR spectroscopy can be used for rapid determination of the concentration of oxalate in human urine samples.

• Childhood Kidney Diseases
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• v.28 no.2
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• pp.74-79
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• 2024

Purpose: To compare the Doppler sonographic findings of the left renal vein (LRV) of children diagnosed with nutcracker syndrome with and without orthostatic proteinuria. Methods: Fifty and 53 consecutive children with and without orthostatic proteinuria, respectively, underwent renal Doppler ultrasonography examinations. The peak velocity (PV) was measured at the hilar portion of the LRV and between the aorta and superior mesenteric artery. Renal Doppler ultrasonography findings and clinical data including urine protein-to-creatinine ratio (UPCR) were compared according to the presence or absence of orthostatic proteinuria. Results: Between the two groups, no significant differences were observed in terms of age or sex. The PV ratio between the aortomesenteric and hilar portions was 7.79±2.65 and 6.32±3.01 in children with and without orthostatic proteinuria, respectively (P=0.009). No significant differences were observed between the two groups in terms of the UPCR in the first morning urine sample. However, the UPCR in the afternoon urine sample was significantly higher in children with orthostatic proteinuria than in those without orthostatic proteinuria (0.49±0.46 mg/mg vs. 0.11±0.04 mg/mg, P<0.001). Furthermore, the PV ratio between the aortomesenteric and hilar portions revealed a positive correlation with the ratio of UPCR of the afternoon to that of first morning urine samples (R=0.21, P=0.034). Conclusions: This study suggests that there can be a significant correlation of the PV ratio between the aortomesenteric and hilar portion of the LRV with orthostatic proteinuria in pediatric patients with nutcracker syndrome.

• The Journal of Korean Medicine
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• v.20 no.2
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• pp.43-53
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• 1999

The purpose of this study is to find out the effects of honey-fried Astragali Radix extract on the nephrotoxicity in rats induced by cyclosporin A(CsA). The experimental rats were divided into three groups of ten. The Normal group was given nothing. The Control group was given only saline water every day for 14 days after the subcutaneous IV injection of 50mg/kg in CsA every other day during a period of 14 days. The sample group was administered 4.8mg/200g of Astragali Radix extract daily for 14 days after the subcutaneous IV injection of 50mg/kg in CsA every other day for 14 days. These groups were observed for 14 days. This experimental research focused on measuring the levels of BUN, creatinine, total protein, sodium, potassium, chloride, AST and ALT in the serum and specific gravity and creatinine in the urine. The results were summarized as follows: 1. Changes in serum The levels of BUN, creatinine. AST and ALT in the serum were significantly decreased in the sample group as compared with those of the control group. Total Protein level in the serum was significantly increased in the sample group as compared with that of the control group. Sodium and Potassium levels in the serum in the sample group were a little lower than those of the control group but no significance was noted. The chloride level in the serum in the sample group significantly increased as compared with that of the control group on the 7th day but in the sample group was significantly decreased as compared with that of the control group on the 14th day 2. Changes in urine Urinary specific gravity in the sample group showed significant increase, compared to the control group on the 7th day but were a little higher than that of the control group on the 14th day. Creatinine level in the urine were a little higher than that of the control group but no significance was found. These results suggest that honey-fried Astragali Radix might be effective on the nephrotoxicity in rats caused by CsA.