• Journal of Microbiology and Biotechnology
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• v.19 no.5
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• pp.447-454
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• 2009

The transport of spermidine into a cyanobacterium, Synechocystis sp. pec 6803, was characterized by measuring the uptake of $^{14}C$-spermidine. Spermidine transport was shown to be saturable with an apparent affinity constant ($K_m$) value of $67{\mu}M$ and a maximal velocity ($V_{max}$) value of 0.45 nmol/min/mg protein. Spermidine uptake was pH-dependent with the pH optimum being 8.0. The competition experiment showed strong inhibition of spermidine uptake by putrescine and spermine, whereas amino acids were hardly inhibitory. The inhibition kinetics of spermidine transport by putrescine and spermine was found to be noncompetitive with $K_i$ values of 292 and $432{\mu}M$, respectively. The inhibition of spermidine transport by various metabolic inhibitors and ionophores suggests that spermidine uptake is energy-dependent. The diminution of cell growth was observed in cells grown at a high concentration of NaCl. Addition of a low concentration of spermidine at 0.5 mM relieved growth inhibition by salt stress. Upshift of the external osmolality generated by either NaCl or sorbitol caused an increased spermidine transport with about 30-40% increase at 10 mosmol/kg upshift.

• Journal of Drive and Control
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• v.19 no.2
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• pp.17-26
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• 2022

Recently, automatic transmissions caused a good improvement in the shift quality of a forklift. An advanced shift control algorithm, which was based on TCU firmware, was applied with embedded control technology and microcontrollers. In the clutch-to-clutch shifting, one friction element is released and the other friction element is activated. During this process, if the release and application timings are not synchronized, an overrun or tie-up occurs and ultimately leads to a shift shock. The TCU, which measures only the speed of the forklift, inevitably applies the open-loop shift control. In this situation, the speed ratio does not change during the clutch fill. The torque phase occurs until the clutch is disengaged. In this study, an offline shift logic of the learning control was proposed. It induced a synchronous shift when the learning control progressed. During this process, the reference current trajectory of the release clutch was corrected and applied to the next upshift. We considered the results of the overrun/tie-up characteristics of the upshift performed immediately before. The vehicle test proved that the deviation in shift quality, which was caused by the difference in the mechanical characteristics of the clutch, could be improved by the learning control.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.48 no.8
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• pp.591-597
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• 1999

The propagation characteristics of high power microwave pulse in an air-breakdown environment are examined. The maximum electron density produced by microwave air-breakdown is limited to $10^6cm^{-3}$ by the tail-erosion effect. Inorder to increase the electron density, the scheme using two pulses intersecting at a desired height is considered. Increasing the carrier frequency, it is shown that microwave pulse can be transferred without the serious erosion in the numerical simulation. This result is useful for the above scheme. Also, an experiment is conducted to show the tail-erosion effect and confirm that a rapidly generated lossy plasma can cause spectral breaking and frequency shift of a high-power microwave pulse. The experimental results are presented by comparing the frequency spectrum of an incident pulse with that of the pulse transmitted through a self-induced air-breakdown environment. The experimental results show that the amount of frequency upshift is co-related with the ionization rate, whereas that of frequency downshift is correlated with the energy losses from the pulse in the self-generated plasma.

• BMB Reports
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• v.39 no.4
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• pp.394-399
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• 2006

The transport of putrescine into a moderately salt tolerant cyanobacterium Synechocystis sp. PCC 6803 was characterized by measuring the uptake of radioactively-labeled putrescine. Putrescine transport showed saturation kinetics with an apparent $K_m$ of $92{\pm}10\;{\mu}M$ and $V_{max}$ of $0.33{\pm}0.05\;nmol/min/mg$ protein. The transport of putrescine was pH-dependent with highest activity at pH 7.0. Strong inhibition of putrescine transport was caused by spermine and spermidine whereas only slight inhibition was observed by the addition of various amino acids. These results suggest that the transport system in Synechocystis sp. PCC 6803 is highly specific for polyamines. Putrescine transport is energy-dependent as evidenced by the inhibition by various metabolic inhibitors and ionophores. Slow growth was observed in cells grown under salt stress. Addition of low concentration of putrescine could restore growth almost to the level observed in the absence of salt stress. Upshift of the external osmolality generated by either NaCl or sorbitol caused an increased putrescine transport with an optimum 2-fold increase at 20 mosmol/kg. The stimulation of putrescine transport mediated by osmotic upshift was abolished in chloramphenicol-treated cells, suggesting possible involvement of an inducible transport system.

• Transactions of the Korean Society of Automotive Engineers
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• v.10 no.3
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• pp.201-208
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• 2002

In this paper, the control strategy of ratio changing system for a metal belt CVT adopting primary pressure regulation is developed, and the shirting performance of pressure regulating type CVT with the suggested control strategy is investigated. The control strategy suggested in this study is composed of 2 feedback loop, one is speed ratio feedback and the other is primary pressure feedback. The pressure feedback is adopted to ensure prohibiting a belt slip during transient period in a fast downshift mode. Simulation results show that the system with suggested control strategy gives appropriate response time and tracking Performance for upshift and also gives a proper primary pressure which can prohibit the belt slip. In addition, it is fecund that the given system has an acceptable servo property in tracking the target speed ratio and robustness for the disturbance of line pressure.

• Journal of Mechanical Science and Technology
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• v.16 no.5
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• pp.639-646
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• 2002

In this paper, thrust equations for a rubber belt CVT are derived by considering the geometry and mechanism of the mechanical actuators. In order to solve the thrust equations, an algorithm to calculate the speed ratio is suggested for the given driver speed and load torque based on the actuator characteristic equations and existing formula for the belt thrust forces. Experiments are performed to investigate the driver speed-load torque-speed ratio characteristics at a steady state. The speed and torque efficiencies are measured and used to modify the actuator equations. It is found that the modified equations well predict the steady state characteristics. In addition, the shift dynamic model for a rubber belt CVT is derived experimentally. Simulation results of the CVT shift dynamics are in good accordance with the experiments and it is noted that different coefficients are required to describe the CVT shift dynamics for the upshift and the downshift.

• Journal of Surface Science and Engineering
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• v.48 no.4
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• pp.163-168
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• 2015

Graphene has attracted much attention due to its remarkable physical properties and potential applications in many fields. In special, the electronic properties of graphene are influenced by the number of layer, stacking sequence, edge state, and doping of foreign elements. Recently, many efforts have been dedicated to alter the electronic properties by doping of various species, such as hydrogen, oxygen, nitrogen, ammonia and etc. Here, we report our recent results of plasma doping on graphene. We prepared mechanically exfoliated graphene, and performed the plasma treatment using ammonia gas for nitrogen doping. The direct-current plasma system was used for plasma ignition. The doping level was estimated from the number of peak shift of G-band in Raman spectra. The upshift of G-band was observed after ammonia plasma treatment, which implies electron doping to graphene.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.5
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• pp.281-288
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• 2002

Recombinant Escherichia coli strain harboring the ${\lambda}$pR-pL promotor and heterologus poly-${\beta}$-hydroxybutyrate (PHB) biosynthesis genes was used to investigate the effect of culture conditions on the efficient PHB production. The expression of phb genes was induced by a temperature upshift from $33^{\circ}C\;to\;38^{\circ}C$. The protein expression levels were measured by using two-dimensional electrophoresis, and the enzyme activities were also measured to understand the effect of culture temperature, carbon sources, and the dissolved oxygen (DO) concentration on the metabolic regulations. AcetylCoA is an important branch point for PHB production. The decrease in DO concentration lowers the citrate synthase activity, thus limit the flux toward the TCA cycle, and increase the flux for PHB production. Since NADPH is required for PHB production, the PHB production does not continue leading the overproduction of acetate and lac-tate. Based on these observations, a new operation was considered where DO concentration was changed periodically, and it was verified its usefulness for the efficient PHB production by experiments.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.22 no.3
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• pp.663-670
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• 1998

Most of feedback shift controllers developed in the past have fixed control parameters tuned by experts using a trial and error method. Therefore, those controllers cannot satisfy the best control performance under various driving conditions. To improve the shift quality under various driving conditions, a new self-organizing controller(SOC) that has an optimal control performance through self-learning of driving conditions and driver's pattern is designed in this study. The proposed SOC algorithm for the shift controller uses simple descent method and has less calculation time than complex fuzzy relation, thus makes real-time control passible. PCSV (Pressure Control Solenoid Valve) control current is used as a control input, and turbine speed of the torque converter is used indirectly to monitor the transient torque as a feedback signal, which is more convenient to use and economic than the torque signal measured directoly by a torque sensor. The results of computer simulations show that an apparent reduction of shift-transient torque is obtained through the process of each run without initial fuzzy rules and a good control performance in the shift-transient torque is also obtained.

• Transactions of the Korean Society of Automotive Engineers
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• v.12 no.4
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• pp.154-162
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• 2004

In this study, a mathematical model for analyzing the shifting transients of the passenger car with an automatic transmission is proposed. The proposed model comprises a power transmission system and a vehicle system, which are coupled. In order to extract the modeling parameters, on-road car test is carried out. The model is composed of a detailed powertrain, an engine/AT housing, a simplified suspension system, tires and a vehicle body model. On the test, the vehicle accelerations and pitch ratio are measured by using accelerometers and a gyro sensor. The speeds, the brake signal, and the throttle position are taken from sensors which already exist in the vehicle. Considering natural ftequencies, which is calculated from the measured accelerations, and the characteristic equation, vehicle model parameters are identified. Dynamic behaviors during upshift or downshift are simulated using the proposed vehicle model. By comparing and analyzing the simulation result and on-road car test data, the vibration of the Engine/AT housing influences the shifting transients. The effect of model parameters are also studied. Among model parameters, the location of engine mountings influences the vibration of the vehicle body.