• Journal of the Korean Society of International Agriculture
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• v.23 no.5
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• pp.537-545
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• 2011

This study was conducted to obtain basic information on physiological and proteomic responses of barley seedlings to salt stress. Shoot dry weight decreased significantly as the level of soil salinity increased. Salt stress-induced decrease of relative shoot dry weight was lower in cv. "Sanglok" than in cv. "Sunwoo". Under the salt stress, SPAD value decreased, and the value was higher in cv. "Sanglok" than in cv. "Sunwoo". Sodium ion content in the leaves increased as NaCl concentration increased, and the content was higher in cv. "Sunwoo" than in cv. "Sanglok". The K⁺/Na⁺ ratio was higher in cv. "Sanglok" than in cv. "Sunwoo". Salt stress-induced alterations in protein expression of the leaves were detected by two dimensional electrophoresis, and 47 protein spots showing altered expression were selected. Among the selected protein spots, 17 protein spots were up-regulated and 28 spots down-regulated in cv. "Sanglok". In cv. "Sunwoo", 14 protein spots were up-regulated and 27 spots down-regulated. Out of 47 deferentially expressed protein spots, 18 protein spots were identified using mass spectrometry and NCBI protein database. Among the identified proteins, ten proteins are known to be involved in various stress responses, but the others are not directly involved in stress responses.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.4
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• pp.330-338
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• 1999

Extensive research has been conducted on effects of drought stress on growth and development of soybean but information is rather restricted on the limited-irrigation system by way of precaution against a long-term drought condition in the future. The experiment for limited-irrigation was conducted in transparent vinyl shelter at Asian Vegetable Research and Development Center (AVRDC), Taiwan in 1997. Two soybean varieties, Hwangkeum and AGS292, improved in Korea and AVRDC, respectively were used for this experiment. The relationships between normalized transpiration rate (NTR) and fraction of transpirable soil water (FTSW) in both varieties were similar that the NTR was unchanged until FTSW dropped to about 0.5 or 0.6. At FTSW less than those values, NTR declined rapidly. Days required to harvest in both varieties were significantly prolonged at IR6 treatment compared to any other treatments. Daily mean transpiration rate was significantly higher at IR5 treatment, as averaged over varieties. Similarly, water use efficiency was also high at 1R5 treatment. In both varieties, seed yield was the greatest at the IR5 treatment, as compared to any other limited-irrigation treatments, due to the increased seed number and high transpirational water use efficiency. The indices of input water and seed yield for the different limited-irrigation treatments against control indicated that Hwangkeum produced 59.6% or 60.7% of seed yield using 36.1% or 44.9% of input water, as compared to control, by irrigation at only R5 or R6 stages, respectively. The AGS292 produced 56.1% of seed yield with 35.4% of input water of control, when irrigated at R5 stage. The results of this study have elucidated that the limited irrigation at R5 stage in soybean can be minimized yield loss with such small quantity of water under the environment of long-term drought stress and the expected shortage of agricultural water in the future.

• Weed & Turfgrass Science
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• v.5 no.2
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• pp.60-64
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• 2016

Investigation on weed flora in Lycium chinense upland fields was conducted to understand the current status of weed flora and establish the control measures. Investigation was conducted twice, May and June on 2015. From this investigation, 91 species of 30 families including 27 exotics were identified into 36 species of annuals, 28 species of biennials and 27 species of perennials. Dominance was the highest with Portulaca loeracea followed by Digitaria ciliaris, Stellaria media, Oxalis corniculata, Acalypha australis, Chenopodium album, Cyperus iria etc. in order. Chenopodium album was the highest in importance analysis and the followings were in order of Conyza canadensis, Conyza bonariensis, Senecio vulgaris, Amaranthus lividus, Sonchus asper etc.

• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.3
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• pp.109-121
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• 2020

Converting the agricultural land-use of rice field to upland has been increasingly conducted as farmers encourages themselves to grow higher value-added crops on rice fields under the policy support. Comparing to rice field, Upland shows different characteristic of discharge due to the slope, scale, and shape of field and characteristics of rainfall event. In this study, we designed the experiment fields reflecting flat-upland characteristics with different land scale, and tried to collect the discharge and load data. Soybeans and corn were selected as target crops considering the possibility of large-scale cultivation and crop demand. The cultivation was conducted during the growth period in 2019 with 3 different field scales. Hence, we have collected the discharge data from 17 rainfall events and the load data for 8 rainfall events. As a result, the magnitude of rainfall events and the discharge duration were found to have a strong positive correlation and field discharge occurred during the period by 55% to 83% of rainfall duration. Besides we found other relationships and characteristics of rainfall event, discharge, and pollutant load and also pointed out that continuous monitoring and more data are required to derive statistically significant results. Compared with slope-field monitoring data obtained from the precedent research, the runoff ratio of the flat-fields was significantly lower than slope-fields. Overall the discharge in the slop and flat-fields shows appreciably different characteristics so that the related researches need to be further conducted to reasonably assess environmental impact of agricultural activities at flat-field.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.2
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• pp.99-105
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• 2012

The main challenges for the development of agricultural bio-degradable mulching film concern the degradation during the lifetime of cultivated crops. A set of rice by-product (rice-hull and rice-bran) based bio-degradable mulching films was developed and tested, following the measurement of standard bio-degradability rate and adaptability in a large scale field experiment. The standard bio-degradability of bio-film passed the KS (Korea standard) regulation. The result of mechanical analysis of bio-degradable mulching film presented a higher mechanical strength and elongation rate compared with polyethylene film. In addition, bio-film could be degraded into fragments within 4 months under the field condition of several upland crops. Bio-degradable mulching film indicated great potential as a new source of agricultural bio-degradable material.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.4
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• pp.336-342
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• 1993

To obtain the basic information necessary for the development of soybean varieties well adaptable to upland-paddy field rotational croppings, the difference of growth characteristics between upland and paddy-field including yield potentials of current recommending soybean varieties were evaluated. The growth characteristics, both above and under-ground, which were measured at flowering stage were generally greater in paddy-field but the number of root nodules was much greater in upland, thus the artificial inoculation was practically recommended for soybean growing in paddy-fields. Mean seed yield was generally higher in paddy-fields than in upland. All soybean varieties showed higher seed yield in the early planting date, April 20, were somewhat susceptible to soybean mosaic virus(SMV), thus they could be escaped from the disasterous endemic necrotic soybean mosaic virus(SMV-N). Soybean varieties showed over 4.0 tons/ha seed yield in the paddy-field were Williams 79, Union, SS77053, and Namhaekong. At the same time, Jangyeobkong and Danyeobkong were the most stable soybean varieties among the tested soybean varieties with less than 10% of coefficient variation values in all planting dates in paddy fields. Compared with Hwangkeumkong which is most widely being cultivated on farmer's fields, soybean varieties showed high yields in paddy-field were higher in plant height, less in the number of branches, and more in the number of nodes on main stem. At the same time, they had medium seed size which would bring the good germination and stands. Disease resistance especially for necrotic soybean mosaic virus was also one of the most decisive factors in seed yields for the early planted soybeans.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2005.10a
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• pp.32-37
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• 2005

Crop evapotranspiration rates of the garlic, potato and carrot were measured in a lysimeter at the National Institute of Subtropical Agriculture. The crop coefficients were calculated using the values of the actually measured evapotranspiration(ETcrop) and the reference crop evapotranspiration (ETo) estimated by the Penman-Monteith equation. The maximum crop coefficients of the garlic, potato, carrot and cabbage were 1.07, 1.07, 0.73 and 0.92 respectively. For the Citrus Aoshima Unshiu and Hallabong in the plastic house, the maximum crop coefficients were 1.38 and 1.29 respectively. Computer program using EXCEL was also developed to estimate the crop water use.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.276-283
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• 2014

This study was conducted in order to investigate the distribution pattern of weeds on upland crop field in northern Gyeonggi-do. The weeds were summarized as 201 taxa including 42 families, 129 genera, 178 species, 1 subspecies, 21 varieties and 1 form. One hundred and thirty one species were classified to annual plants, accounting for 65.1% and 70 species were classified to perennials, accounting for the rest of 34.9%. Compositae was dominant family (21%), followed by Gramineae (12%), Polygonaceae (7%) and Brassicaceae (5%). Among the weeds appearing in the fields of northern Gyeonggi-do, the invasive weeds were classified to 62 species in18 families. The most dominant weed species in the fields were Portulaca oleracea, followed by Echinochloa crus-galli, Amaranthus lividus. Detrended correspondence analysis for investigation of occurrence pattern of weeds by crops revealed the occurrence pattern of weed species in adlay field were different from those in other crop fields. This information could be useful for establishment of weed control methods in northern Gyeonggi-do.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.284-291
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• 2014

We surveyed the distribution of exotic weeds in the 350 upland crop fields of Gyeonggi-do. The exotic weeds were summarized as 78 taxa including 19 families, 52 genera, 74 species and 4 varieties. Among the total exotic weeds, the summer annuals were 48.7%, the winter annuals 28.2%, and the perennials 23.1%. Compositae was dominant family (32%), followed by Gramineae (8%), Polygonaceae (8%) and Malvaceae (7%). Dominant exotic weeds in northern Gyeonggi-do were Amaranthus lividus, Ambrosia trifida, Chenopodium album, Bidens frondosa and Erigeron Canadensis, and in southern Gyeonggi-do were Chenopodium album, Erigeron Canadensis, Erigeron annuus, Taraxacum officinale and Galinsoga ciliate. The most dominant exotic weeds in the Gyeonggi-do were Chenopodium album, followed by Amaranthus blitum, Erigeron canadensis. Canonical correspondence analysis for investigation of correlation between exotic weeds occurred in northern and southern Gyeonggi-do showed that the exotic weeds in northern Gyeonggi-do were more diverse then in southern Gyeonggi-do. This information could be useful for establishment of exotic weed control methods in Gyeonggi-do.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.197-205
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• 2017

Exposure of Jurkat T cell clone (J/Neo cells) to acacetin (5,7-dihydroxy-4'-methoxyflavone), which is present in barnyard millet (Echinochloa esculenta (A. Braun)) grains, caused cytotoxicity, enhancement of apoptotic $sub-G_1$ rate, Bak activation, loss of mitochondrial membrane potential (${\Delta}{\Psi}m$), activation of caspase-9 and caspase-3, degradation of poly(ADP-ribose) polymerase, and FITC-Annexin V-stainable phosphatidylserine exposure on the external surface of the cytoplasmic membrane without accompanying necrosis. These apoptotic responses were abrogated in Jurkat T cell clone (J/Bcl-xL) overexpressing Bcl-xL. Under the same conditions, cellular autophagic responses, including suppression of the Akt-mTOR pathway and p62/SQSTM1 down-regulation, were commonly detected in J/Neo and J/Bcl-xL cells; however, formation of acridine orange-stainable acidic vascular organelles, LC3-I/II conversion, and Beclin-1 phosphorylation (Ser-15) were detected only in J/Neo cells. Correspondingly, concomitant treatment with the autophagy inhibitor (3-methyladenine or LY294002) appeared to enhance acacetin-induced apoptotic responses, such as Bak activation, ${\Delta}{\Psi}m$ loss, activation of caspase-9 and caspase-3, and apoptotic $sub-G_1$ accumulation. This indicated that acacetin could induce apoptosis and cytoprotective autophagy in Jurkat T cells simultaneously. Together, these results demonstrate that acacetin induces not only apoptotic cell death via activation of Bak, loss of ${\Delta}{\Psi}m$, and activation of the mitochondrial caspase cascade, but also cytoprotective autophagy resulting from suppression of the Akt-mTOR pathway. Furthermore, pharmacologic inhibition of the autophagy pathway augments the activation of Bak and resultant mitochondrial damage-mediated apoptosis in Jurkat T cells.