• 약학회지
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• 제29권2호
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• pp.55-61
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• 1985

The effect of dietary fat on the immunotoxicity of chloramphenicol was investigated in mice, sensitized and challenged with sheep red blood cells. Chloramphenicol suppressed more cell-mediated immunity than humoral immunity. The saturated fat diet elevated humoral and cell-mediated immunotoxicity, whereas the unsaturated fat diet decreased humoral immunotoxicity, but elevated cell-mediated immunotoxicity of chloramphenicol. Especially, the normal diet in saturated and unsaturated fat restored immunosuppressive effect of chloramphenicol compared to the saturated fat diet and the unsaturated fat diet.

• Journal of Nutrition and Health
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• 제33권4호
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• pp.386-394
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• 2000

This study was conducted to investigate the effects of shellfish hydrolysate on lipid metabolism in rats fed high fat diet. Male Sprague-Dawley rate weighting approximately 110g were fed basal control diet, high fat diet and high fat diet plus 4 different shellfish hydrolyates for 4 weeks. The shellfish hydrolysates from the different sources, were oyster, hard-shelled mussel, little neck clam and march clam. After 4 weeks, serum GOT, GPT, ${\gamma}$-GTP, triglyceride and total cholesterol was significantly decreased in shellfish hydrolysates supplementation with high fat diet compared to basal control and high-fat group(p<0.05). The total lipid and cholesterol content in liver showed significant decrease(p, 0.05). There were no different in serum GPT, HDL-cholesterol, liver total cholesterol and lipid of rats between basal control diet and high-fat diet. The unsaturated fatty acids, specific components of shellfishes were a little components in shellfish hydrolysate as they were a low and not different among the groups and were most well reflected in liver and plasma. Considering digestive and absorptive process of in human body, it was assumed that the hypolipidemic effect of shellfish was not under the influence of unsaturated fatty acids but the other components, peptides, taurine and betaine and so on was detected in the process of hyperlipidemia induced by high-fat diet.

• Journal of Nutrition and Health
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• 제34권8호
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• pp.858-864
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• 2001

The study was designed to observe an antioxidant activities of conjugated linoleic acid(CLA) in rat liver by determining the activities of antioxidative enzymes(superoxide dismutase, glutathione peroxidase, catalase) and the levels of tocopherol and thiobarbituric acid reactive substance(TBARS). Male Sprague Dawley rats at weeks-old were divided into groups according to the types of dietary fat(beef tallow and fish oil) and then each group was subdivided into groups depending on CLA supplement. All rats were fed experimental diet containing 12% total fat including 1% CLA by weight for 30 weeks. CLA supplemented to beef tallow diet did not have significant effect on the level of TBARS and tocopherol. The level of TBARS was significantly increased in fish oil diet(highly unsaturated fat diet), but its level was significantly reduced by increasing SOD and GSH-Px activities when CLA was supplemented to fish oil diet so that CLA showed a sparing action of tocopherol in tissue. CLA did not have significant effect on peroxisomal catalase activities, but its activity was significantly increased when TBARS production was high in the fish oil diet. CLA could be incorporated into phospholipid of microsomal membrane, and interfered the conversions of C18 : 0 into C18 : 1 and C18 : 2 into C20 : 4 in liver. In conclusion, CLA had an antioxidant activities depending on the type of fat in diet. Therefore, it could be recommended to use CLA when highly unsaturated fat was used in meal preparation.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.838-844
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• 2008

This experiment was conducted to evaluate the effect of supplementing a fat diet with selenium (Se) and vitamin E on performance of cows, blood antioxidant status and milk fatty acid composition. Sixty-three lactating Holstein cows were randomly divided into seven groups of nine cows each and each group was fed one of the following diets: i) a basal diet (control); ii) a basal diet with 0.15 mg Se/kg DM (LSe); iii) a basal diet with 0.3 mg Se/kg DM (HSe); iv) a basal diet with 5,000 IU/cow d vitamin E (LVE); v) a basal diet with 10,000 IU/cow d vitamin E (HVE); vi) a basal diet with 0.15 mg Se/kg DM and 5,000 IU/cow d vitamin E (LSeVE); vii) a basal diet with 0.3 mg Se/kg DM and 10,000 IU/cow d vitamin E (HSeVE). Milk fat percentage and conjugated linoleic acid (CLA) yield in HVE and HSeVE diets increased (p<0.05) compared with the control diet. In milk fat, dietary supplementation of Se tended to increase the proportion of the sum of unsaturated fatty acids (UFA) and significantly decreased (p<0.05) the proportion of the sum of saturated fatty acids (SFA). In addition, compared with the control, thiobarbituric acid reactive substances (TBARS) content was lower and glutathione peroxidase (GSH-Px) was higher when fat diets were supplemented with Se. Our data showed that supplementation with Se and/or VE improved these nutrients in blood and milk. The results indicated that fat diets supplemented with Se improved both antioxidant status in blood and fatty acids in milk fat, and fat diets supplemented with vitamin E alleviated milk fat depression. Therefore, the combination of Se and vitamin E caused synergistic effects on the nutritional quality of milk fat and performance of cows fed a fat diet.

• 대한수의학회지
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• 제30권4호
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• pp.407-412
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• 1990

This study was designed to elucidate the effects of administration with dietary iron and fat-free diet on the contents of unsaturated fatty acid in phospholipid molecules, vitamin E contents and malondialdehyde contents in liver, kidney, muscle and testis of the male rats. The rats were divided into 3 experimental groups, namely, control, iron injection and fat-free diet administration groups. The control group was fed with normal diet, iron injection group injected intraperitoneally 20mg of ferric hydroxide/100g of body weight 20 times every 3 days and fat-free diet group administered lipid extraction diet with hexane in normal diet. All experimental groups were maintained for 60 days with feeding on the respective ration. The results obtained were summarized as follows: 1. In the mean contents of unsaturated fatty acid in phospholipid of liver, kidney, muscle and testis among groups, control group was 21.31mg/g, 19.38mg/g, 1.67mg/g, 13.68mg/g, iron injection group was 13.83mg/g, 16.53mg/g, 0.71mg/g, 10.11mg/g and fat-free diet group was 21.07mg/g, 19.38mg/g, 1.49mg/g and 13.40mg/g, respectively. 2. In the mean contents of vitamin E in liver, kidney, muscle and testis among groups, control group was 6.77mg/g, 1.93mg/g, 0.12mg/g, 0.17mg/g, iron injection group was 3.16mg/g, 0.86mg/g, 0.07mg/g, 0.09mg/g and fat-free diet group was 7.41mg/g, 1.50mg/g, 0.11mg/g and 0.16mg/g, respectively. 3. In the mean contents of malondialdehyde in liver, kidney, muscle, testis and serum among groups, control group was 11.29nM/0.1g, 23.25nM/0.1g, 42.47nM/0.1g, 7.01nM/0.1g, 4.33nM/ml, iron injection group was 34.98nM/0.1g, 40.55nM/0.1g, 72.21nM/0.1g, 12.26nM/0.1g, 11.27nM/ml and fat-free diet group was 8.07nM/0.1g, 20.63nM/0.1g, 39.92nM/0.1g, 6.95nM/0.1g and 4.27nM/ml, respectively.

• 대한한방내과학회지
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• 제21권3호
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• pp.461-465
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• 2000

The effects of 3% Cortex Eucommiae extracts on serum lipid contents were evaluated in rats. Forty-two male Sprague-Dawley rats weighing $100{\pm}10g$ were divided into seven groups and fed different fat diets for four weeks. Each group was administered with following group: Control, basal fat diet, saturated fat diet and unsaturated fat diet respectively was fed 1% cholesterol, Cortex Eucommiae extracts 3%. The contents of serum lipid contents triglyceride in the basal fat diet group were higher than those of control group. The contents of total-cholesterol in serum of the saturated fat diet group was significantly higher than other group. The contents of HDL-cholesterol in serum of the 3% Cortex Eucommiae extract group were higher than those other fat diet group. The contents of LDL-cholesterol in serum of the 3% Cortex Eucommiae ex1ract group was lower than other fat diet groups. These results suggest that 3% Cortex Eucommiae extract group may reduced elevated levels of serum lipid contents in rats different fed fat diet.

• 한국농촌생활과학회지
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• 제6권1호
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• pp.31-39
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• 1995

This study was performed to observe how soy protein and calcium in rats fed unsaturated fat-enriched diet affect the total lipid and cholesterol contents of blood and tissues male Sprague-Dawley rats weighing approximately 220g were fed four purified diets which contained 18% (w/w) corn oil, 1% (w/w) cholesterol, two sources of protein : casein or isolated soy protein (ISP) and two levels of dietary calcium : 0.1% or 1.0%, first for four weeks (Expt. 1) and second for eight weeks (Expt. 2). The contents of total lipid, cholesterol and triglyceride in blood, liver, heart and feces were determined. After four weeks feeding, the serum lipid and cholesterol concentration was not significantly different among the groups. After eight weeks feeding, these concentrations were significantly lower in ISP-High calcium group. The serum lipid concentration was influenced by dietary protein sources and calcium levels. These results indicated that the hypolipidemic effects of soy protein and high calcium intake were partly due to decrease in lipid absorption and these effects were not detected at 4 weeks feeding but 8 weeks feeding.

• 한국식품과학회지
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• 제31권3호
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• pp.809-814
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• 1999

각 지방 식이를 달리 처리하여 물과 대두 추출물을 섭취한 흰쥐의 혈청 지질 함량에 미치는 영향을 조사하기 위해 체중 $100{\pm}10g$의 Sprague-Dawley계 수컷 흰쥐 98마리를 콜레스테롤 비투여군, 표준지방식이, 동물성 지방식이, 식물성 지방식이, 고지방식이에 각각 1% 콜레스테롤을 첨가하여 물과 대두 추출물을 달리 섭취하여 처리한 실험군을 14개군으로 분류하여 4주 동안 사육하였다. 체중 증가량은 각 지방식이를 달리 하여 물만 섭취한 군과 대두 추출물 처리를 달리하여 섭취한 군들 사이에서 대두 추출물 군이 약간 증가하였으나 유의적인 큰 차이는 없었다. 혈청 중 중성지질과 콜레스테롤 함량에서 물만 섭취한 군에 비하여 대두추출물군에서 낮은 함량을 나타냈다. HDL-, LDL-cholesterol은 고지방식이와 동물성 지방식이에서 대두콩 추출물의 투여후 혈중 지질 개선 효과가 뚜렷하게 관찰되었고, 특히 식물성 지방 식이와 대두 추출물을 같이 투여한 군은 상승 작용을 가져와 혈중 지질 증가 억제 효과가 컸다.

• Journal of Nutrition and Health
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• 제26권5호
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• pp.566-577
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• 1993

The study was to compare the effect of dietary fatty acids on fatty acid profile in tissue and the status of tocopherol and lipid peroxidation, and superoxide dismutase and glutathione peroxidase activities at two fat levels. Male Sprague Dawley rats weighing average 350g(17 weeks) were fed either low fat(LF, 4.3% w/w, 10% kcal) or high fat(HF, 20.8%, w/w, 40% kcal)diet for 6 weeks. The fats used were beef tallow as a source of saturated fatty acid, corn oil for n-6 linoleic acid, perilla oil for n-3 $\alpha$-linolenic acid and fish oil for n-3 eiocosapentatenoic acid(EPA) and n-3 docosahexaenoic acid(DHA). Palsma tocopherol was significantly reduced by fish oil compared to beef tallow at body fat level. However, there was no significant effect on the levels of plasma MDA, RBC MDA and tocopherol, and RBC hempolysis by the type and amount of dietary fat. The peroxidizibility index of fatty acid profile in plasma and liver was increased and liver MDA level was significantly increased by fish oil when dietary fat level was increased. The activities of SOD and GSHPx tended to be increased by perilla oil and fish oil at both fat oil significantly reduced the incorpration of c20:4 and increased the incorporation of c20:5 into liver compared to corn oil. The incorporation of n-3 fatty acids into tissue by perilla oil rich in $\alpha$-linolenic acid was significantly higher tan corn oil and its effect was improved with higher amount of perilla oil in diet by high fat diet. Overall, the lipid peroxidation of tissue could be prevented by tocopherol supplementation when dietary fat level was low in diet. However, at high fat diet, tocopherol supplementation might not be enough to prevent the lipid peroxidation in tissue since the potential for lipid peroxidation was tended to be increased with higher incorporation of higher unsaturated n-3 fatty acids into tissue. Therefore, it could not be recommended to consume large amount of fish oil even with excess amount of tocopherol supplemented to the high fat diet.

• 한국응용과학기술학회지
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• 제6권1호
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• pp.75-85
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• 1989

This paper in the feeding Selenium added high fat diet, have an aim to find an effect of the white rat'food intake, body weight and level of serum lipid. This experimentations com to the conclusion that Se. added high fat diets, to say again, soybean oil added high fat diets that expecially is contained unsaturated high fatty acid, improve a general diet-utility factor, and decrease a body weight and coronary heart desease factor of the serum have an effect to prevent them from C H D by increasing anti - arterioscleroticfactor.