• Title/Summary/Keyword: Unrecorded yeast

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Characterization of the Unrecorded Wild Yeasts from the Water and Riverside Soils of Daejeoncheon and Gapcheon in Daejeon Metropolitan City, Korea (대전광역시 대전천과 갑천의 물과 주변 토양들로부터 분리한 국내 미기록 야생효모들의 특성)

  • Han, Sang-Min;Lee, Sang-Yeop;Kim, Ha-Kun;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.45 no.2
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    • pp.153-159
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    • 2017
  • We isolated 120 strains of wild yeasts from the water and riverside soils of Daejeoncheon and Gapcheon in Daejeon, Korea. We identified Debaryomyces udenii JSF601, Kazachstania telluris JSF602, Trichosporon faecale JSF614, Candida infanticola WJSL0039, Candida palmioleophila WJSL0048, Pichia spartinae WJSL0087, and Trichosporon coprophilum WJSL0093 from Daejeoncheon, and Leucosporidium golubevii WJSL0108 and Ustilentyloma graminis WJSL0118 from Gapcheon, as newly recorded yeast strains in Korea and investigated their microbiological characteristics. All of these previously unrecorded yeasts were oval- or ellipsoidal-shaped with ascospores, except C. infanticola WJSL0039, C. palmioleophila WJSL0048, and D. udenii JSF601. L. golubevii WJSL0108 and U. graminis WJSL0118 grew in vitamin-free medium, and C. infanticola WJSL0039 was halotolerant and grew in 10% NaCl-containing YPD broth. K. telluris JSF602 and P. spartinae WJSL0087 were thermophilic yeasts, which grew at $37^{\circ}C$.

Isolation and Characterization of Wild Yeasts from Water and Riverside Soils of Geumgang Midstream in Gongju City, Korea (금강 중류 공주시 주변 물과 토양으로부터 야생효모의 분리 및 국내 미기록 효모 특성)

  • Han, Sang-Min;Kim, Ji-Yoon;Lee, Hyang Burm;Kim, Ha-Kun;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.46 no.2
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    • pp.98-104
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    • 2018
  • The goal of this study was to elucidate wild yeast diversity of the midstream region of the Geumgang river, Gongju, Chungnam province, Korea. Fifty strains of 24 species of wild yeasts were isolated from 45 water and soil samples among which Cryptococcus spp. (11 strains), Cryptococcus magnus (7 strains), Rhodotorula spp. (9 strains), and Hanseniaspora spp. (6 strains) were dominant. Four species, Candida chauliodes WJSF 0201, Candida oleophila WJSF 0202, Candida catenulata WJSF 0203, and Candida jaroonii WJSF 0204, represented newly recorded yeasts in Korea. All of these unrecorded yeasts were oval in shape, formed ascospores and pseudomycelium, and grew in vitamin-free medium. Candida oleophila WJSF 0202 was thermophilic which can grow at $37^{\circ}C$.

Characteristics and Physiological Functionalities of Unrecorded Yeasts from Wild Flowers of Seonyudo in Jeollabuk-do, Korea (전라북도 선유도일대 야생화에서 분리한 국내 미기록 효모들의 특성 및 생리기능성)

  • Hyun, Se-Hee;Han, Sang-Min;Lee, Jong-Soo
    • Microbiology and Biotechnology Letters
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    • v.42 no.4
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    • pp.402-406
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    • 2014
  • Six kinds of newly recorded yeasts such as Rhodosporidium diobovatum SY4-2, Cryptococcus bestiolae SY7-1, Kazachstania unispora SY14-1, Kazachstania servazzii SY14-3, Pichia holstii SY20-2 and Cryptococcus tephrensis SY26-1 were screened from sixty one yeasts derived from wild flowers found in Seonyudo, Gogunsanyeoldo, Jeollabuk-do, Korea. All of them grew in 50% glucose-containing yeast extract-peptone-dextrose (YPD) broth and Pichia holstii SY20-2 was also halophile, growing in 20% NaCl-containing YPD broth. All of them, except Cryptococcus tephrensis SY26-1, were assimilated to glucose. Cell-free extract from Kazachstania servazzii SY14-3 showed the highest 98.6% of ${\alpha}$-glucosidase inhibitory activity and maximal production of the ${\alpha}$-glucosidase inhibitor was obtained with 24h incubation at $30^{\circ}C$. The antihypertensive angiotensin I-converting enzyme inhibitory activity of the unrecorded yeasts were showed 58.6-80.4% in their supernatants.

Characteristics of Unrecorded Yeasts, Rhodosporidium fluviale, Rhodosporidium paludigenum, Candida sp. 80-J-3 and Kluyveromyces thermotolerans Isolated from Wild Flowers in Korea (국내 야생화들로부터 국내 미기록 효모, Rhodosporidium fluviale, Rhodosporidium paludigenum과 Candida sp. 80-J-3, Kluyveromyces thermotolerans의 특성)

  • Hyun, Se-Hee;Lee, Hyang Burm;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.41 no.3
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    • pp.181-184
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    • 2013
  • Rhodosporidium fluviale 201-C-1 and Kluyveromyces thermotolerans 150-JE-2(1) were isolated from wild flowers in Geumsan-gun, Chungcheongnam-do and Wando-gun, Jeollanam-do, respectively. Rhodosporidium paludigenum 86-J-1 and Candida sp. 80-J-3 were also isolated from flowers in Jeju island, Korea. Characteristics for these yeasts were not reported before. Therefore, morphological and physiological characteristics for these yeasts were investigated in this study. R. fluviale 201-C-1 was halophilic asporogenous yeast and formed pseudomycelium. It also grew at $30^{\circ}C$ and pH 4.0-6.0. K. thermotolerans 150-JE-2(1) was sugar-tolerant and halotolerant asporogenous yeast. It formed pseudomycelium and showed positive urease activity. Candida sp. 80-J-3 and R. paludigenum 86-J-1 were asporogenous yeasts, and grew well in yeast extract-peptone dextrose(YPD) medium, potato-dextrose medium as well as vitamin-free medium and YPD medium containing 5% NaCl.