• Plant Biotechnology Reports
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• v.3 no.2
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• pp.147-155
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• 2009

Oryza grandiglumis Chitinase IVa (OgChitIVa) cDNA encoding a class IV chitinase was cloned from wild rice (Oryza grandiglumis). OgChitIVa cDNA contains an open reading frame of 867 nucleotides encoding 288 amino acid residues with a predicted molecular weight of 30.4 kDa and isoelectric point of 8.48. Deduced amino acid sequences of OgChitIVa include the signal peptide and chitin-binding domain in the N-terminal domain and conserved catalytic domain. OgChitIVa showed significant similarity at the amino acid level with related monocotyledonous rice and maize chitinase, but low similarity with dicotyledoneous chitinase. Southern blot analysis showed that OgChitIVa genes are present as two copies in the wild rice genome. It was shown that RNA expression of OgChitIVa was induced by defense/stress signaling chemicals, such as jasmonic acid, salicylic acid, and ethephon or cantharidin and endothall or wounding, and yeast extract. It was demonstrated that overexpression of OgChitIVa in Arabidopsis resulted in mild resistance against the fungal pathogen, Botrytis cinerea, by lowering disease rate and necrosis size. RT-PCR analysis showed that PR-1 and PR-2 RNA expression was induced in the transgenic lines. Here, we suggest that a novel OgChitIVa gene may play a role in signal transduction process in defense response against B. cinerea in plants.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.4
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• pp.564-572
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• 2015

Nutritional compositions, volatile compounds, and sensory attributes of Cynanchi wilfordii Radix were analyzed in order to examine its practical utilization as a food resource. In the proximate analysis, protein and lipid contents were shown to be 14.6 and 5.0 mg/100 g, respectively, in C. wilfordii Radix. Potassium was the most predominant mineral (809 mg/100 g), as determined by inductively coupled plasma-optical emission spectrometry in parallel with microwave acid digestion. Total phenolic content was found to be 410 mg/100 g. Further, arginine and linoleic acid were the most abundant amino acid and fatty acid of C. wilfordii Radix, respectively. To examine its functional properties, classical 2,2-diphenyl-1-picrylhydrazyl (DPPH) analysis was performed. As a result, the concentration of C. wilfordii Radix required to scavenge 50% of DPPH radicals was 1.16 mg of dried material. Lastly, in olfactory and sensory tests, ${\beta}$-eudesmol (woody odor) was the major flavor compound responsible for the bitter taste and sensory attributes of C. wilfordii Radix. Taken altogether, the above results provide important preliminary results for utilization of C. wilfordii Radix as a food resource.

• 한국노년학
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• v.30 no.1
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• pp.221-240
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• 2010

Despite a growing trend in elder mistreatment, research about the problem and its effects on the victims has been sparse. Notably missing are the perspectives of older adults themselves, whose perceptions and responses to elder mistreatment are greatly affected by social and cultural context. The purpose of this study is to examine factors associated with older persons' perceptions of elder mistreatment and subsequent help-seeking behaviors. Six hypothetical scenarios featuring elders were the basis of interviews with 124 older persons, drawn by a quota sampling strategy. Findings indicated that older persons perceived situations of physical abuse (87.9%), financial abuse (86.3%), and psychological abuse (66.1%) to be elder mistreatment, yet respondents showed less sensitivity to elder mistreatment that took the form of physical mistreatment within a couple (47.6%), neglect (40.3%), and self-neglect (16.9%). Certain scenarios yielded less intention to seek help: namely, physical mistreatment within a couple, neglect, and self-neglect. Older persons' personal characteristics, social factors, and cultural factors exerted significant influence on both perception and help-seeking behavior. Perception was identified as a key factor that significantly influenced help-seeking behavior. Findings point to awareness of cultural and social context for success in elder mistreatment prevention, intervention, and policy design for this population.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.3
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• pp.202-211
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• 2011

In this study the seasonal and spatial pattern of mercury (Hg) concentrations in multimedia environments were investigated in Lake So-Yang. Total mercury (TM) in water column greatly enhanced as turbidity and particulate organic carbon (POC) increased due to the severe runoff, suggesting that most of Hg existed as particulate Hg in Lake Soyang. We also collected 22 species of fish in Lake Soyang and Han River, and the average total mercury in fish was 0.073 ppm, lower than both Korean (0.5 ppm) and EPA criteria (0.3 ppm). However when considering the amount of fish intake for Korean the Hg criterion in fish must be more stringent than current value, and the advisory for fish consumption should be made. Hg in fish considerably varied with sampling sites, having the highest in the upper region (Yang-gu) and the lowest in downstream (Han River). This spatial variation was possibly derived by "bloom dilution" associated with high phosphorus loading or elevated DOC concentrations, or both. Total mercury in sediment varied from 69.9 to $98.3{\mu}g/kg$, which was relatively lower than those measured in Voyageurs National Park in Minnesota in USA ($102{\sim}364{\mu}g/kg$).

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.21
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• pp.9417-9421
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• 2014

Background: Esophageal cancer is one of the most frequently occurring malignancies and the seventh leading cause of cancer-related deaths in the world. The esophageal squamous cell carcinoma (ESCC) is the most common histological type of esophageal cancer worldwide. Materials and Methods: Our goal in this study was to detect phospholipase A2 Group IIA (PLA2G2A) and cyclooxygenase-2 (COX-2) immuno-expression in ESCC in a high-risk population in China. Results: Positive expression of PLA2G2A protein was observed in 57.2% (166/290) of the cases, while COX-2 was found in 257 of 290 samples (88.6%), both PLA2G2A and COX-2 being expressed in 153 cases (52.8%), with a significant agreement (Kappa=0.091, p=0.031).Overexpression of PLA2G2A was significantly correlated with the depth of invasion (p=0.001). Co-expression of PLA2G2A and COX-2 not only significantly correlated with the depth of invasion (p=0.004) but also with TNM stage (p=0.04). Conclusions: Our results showed that in patients with ESCC, PLA2G2A overexpression and PLA2G2A co-expression with COX-2 is significantly correlated with advanced stage. The biological role and pathophysiologic regulation of PLA2G2A and COX-2 overexpression in ESCC deserve further investigation.

• Annals of Clinical Neurophysiology
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• v.12 no.1
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• pp.21-26
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• 2010

Background: Although quantitative sensory test (QST) is being used with increasing frequency for measuring sensory thresholds in clinical practice and epidemiologic studies, there has been no age-matched normative data in Korean adults. The objective of this study is to evaluate the value of QST in diabetic polyneuropathy with normal range in Korean adults. Methods: The Computer Aided Sensory Examination IV 4,2 (WR Medical Electronics Co., Stillwater, Minnesota, U.S.A.), with 4,2,1 stepping algorithm was used to determine vibration and cold perception threshold in 70 normal controls and 19 patients with diabetic polyneuropathy aged from 21 to 79 years. The data were used to define age-matched upper and lower normal limits and normal range of side to side difference. We also evaluated the duration of diabetes, serum HbA1C level, and findings of nerve conduction study (NCS) and QST in patients with diabetic polyneuropathy. Results: In normal adults, sensory thresholds slightly increased with age, and a slight side-to-side difference was observed. The diagnostic sensitivity of QST was not higher than NCS in patients with diabetic polyneuropathy (36.8% vs. 42.1%, p=0.716), especially among elderly patients. Conclusions: QST might be used as a complementary test for NCS in the diagnosis of diabetic polyneuropathy. Although the QST is a simple method for the evaluation of peripheral nerve function, there are some limitations. Most of all, because the QST measuring is dependent on the subjective response of patients, the degree of concentration and cooperation of the patients can significantly affect the result. And thus, attention should be paid during the interpretation of QST results in patients with peripheral neuropathy.

• Journal of Oriental Neuropsychiatry
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• v.31 no.3
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• pp.197-211
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• 2020

Objectives: The purpose of this study was to introduce the progress of treatment and improve clinical use after conducting Mentalizing the Rooms of Mind, the main technique of Mindfulness & Loving Beingness psychotherapy, for a patient with panic disorder. Methods: We conducted a Mentalizing the Rooms of Mind for 10 sessions on an age 23 female diagnosed with panic disorder based on the DSM-5 diagnostic criteria. After receiving consent from the subject, through chart review, the progress of treatment was observed focusing on the MMPI-2 and CSEI-s (The Core Seven Emotions Inventory-short form) conducted pre- and post-treatment. This study was approved by the Institutional Review Board of Wonkwang University Sanbon Hospital (WMCSB202007-55). Results: 1. The MMPI-2 clinical scales of an age 23 female with panic disorder showed a 7 (Pt)-1 (Hs)-3 (Hy) profile pre-treatment, but for post-treatment, the scale showed 1 (Hs)-3 (Hy) profile, and the 7 (Pt) scale showed significant decline. In the MMPI-2 reconstructed clinical scales, RC7 (Dysfunctional Negative Emotions) and RC8 (Aberrant Experiences) showed significant decline. 2. In the pre- and post-treatment MMPI-2 content scales, Anxiety, Fears, Obsessiveness, Social Discomfort, and Work Interference scores decreased, showing overall positive stability. On the MMPI-2 supplementary scales, the Anxiety and Post-Traumatic Stress Disorder scores decreased, and the Ego Strength increased, resulting in improved overall psychological adaptation. 3. Pre- and post-treatment of an age 23 female with panic disorder, CSEI-s showed significant decline of 恐, 驚, 悲, and 思. So it seems that the emotions caused by Chiljeongsang (七情傷) were more stable than before treatment. Conclusions: As shown above, the treatment of panic disorder through Mentalizing the Rooms of Mind, a major technique of Mindfulness & Loving Beingness psychotherapy, showed positive changes in MMPI-2 as well as improvement of the subjective symptoms. Thus, Mentalizing the Rooms of Mind has high clinical use, and it seems that it is necessary to create a manual for this in the future.

• Applied Biological Chemistry
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• v.38 no.2
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• pp.123-128
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• 1995

Several factors affecting on transformation efficiency were studied to establish a Agrobacterium rhizogenes mediated system for the transformation of Populus species, and We could obtaine tansgenic plantlets expressing the introduced gene. Leaf sections were more sensitive than stem sections to kanamycin and thought to be better material for transformant screening. The bacterial density did not affect on the efficiency of transformation over the range of $4{\times}10^5{\sim}7{\times}10^9\;cfu$. The optimum period for co-cultivation was one day or shorter. Both of the optimum concentrations of cefotaxime and ampicillin in the medium were $250\;{\mu}g/ml$ for elimination of bacteria from the inoculated leaf sections. The addition of acetosyringone in the bacterial culture medium increased transformation rate, and the highest rate was obtained at $50\;{\mu}M$ of acetosyringone. The transformed galls could be selectively induced and gown on the growth regulator-free medium or on the medium containing $100\;{\mu}g/ml$ or higher contrition of kanamycin. The roots were induced from the galls incited by A. rhizogenes within 3 weeks on the growth regulator-free medium as well as on the medium containing growth regulators. The plantlets were regenerated from the galls cultured for 6 weeks on the medium containing 0.05 mg/ml of NAA and 0.5 mg/ml of BA. The expressions of the introduced opine gene in the transformed galls and plantlets were confirmed by the analysis of agropine and mannopine.

• Journal of Yeungnam Medical Science
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• v.16 no.2
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• pp.244-254
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• 1999

Objectives: This study investigated the psychological characteristics of patients with functional dyspepsia. Methods: The subjects included ninety patients with functional dyspepsia and sixty four psychiatric out-patients. We administered Minnesota Multiphasic Personality Inventory(MMPI) and Illness Behavior Questionnaire(IBQ). Results: There were no significant differences between the patients with functional dyspepsia and the psychiatric out-patients by MMPI. Two groups both showed a higher distribution in hypochondriasis, depression, hysteria subscales than in any other subscales. The patients with functional dyspepsia showed lower scores in disease conviction and affective disturbance subscales in IBQ than the psychiatric out-patients. The distribution of scores of the other IBQ subscales were similar between the patients with functional dyspepsia and the psychiatric out-patients. The patients with functional dyspepsia were divided into three groups for the Multivariate cluster analysis: normal, similar to psychiatric out-patient, and severe neurotic. The severe neurotic group showed higher scores in hypochondriasis, depression, hysteria, psychathenia, and schizophrenia subscales in MMPI and showed significant different scores in affective disturbance, disease conviction, psychological and somatic concerns, affective disturbance, denial, and irritability subscales in IBQ. Conclusion: If patients with functional dyspepsia show severe neurotic behavior, such as those in, they would need appropriate psychiatric intervention.

• The Journal of Korean Academy of Prosthodontics
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• v.45 no.4
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• pp.457-468
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• 2007

Statements of problem: The fracture of acrylic resin dentures remains an unsolved problem. Therefore, many investigations have been performed and various approaches to strengthening acrylic resin, for example, the reinforcement of heat-cured acrylic resin using glass fibers, have been suggested over the years. Silane is important for bonding between glass fiber and resin. Purpose: The aim of the present study was to investigate the effect of various silane on the strength of PMMA resin and roughness of resin-glass fiber complex after abrasion test. Material and methods: 3mm glass fiber (Chopped strand, Hankuk fiber Co., Milyang, Korea) was treated with 3 kinds of silane (MPS, EPS, APS) (Sila-ace, Chisso chemical, Tokyo, Japan) and mixed with PMMA resin(Vertex RS, Vertex Dental B.V., Zeist, Netherlands). Transverse strength and Young's modulus was measured using Instron (Instron model 4466, Instron, Massachusetts, USA). After abrasion test (The 858 Mini Bionix II Test System, MTS System Co., Minnesota, USA) surface roughness was evaluated using tester (Form Talysurf plus, Taylor Hopson Ltd., Leicester England). Examination of scanning electron microscope was also performed. Results: Within this study, the following conclusions were drawn. 1. Surface treatment of glass fiber with MPS and APS increased transverse strength of PMMA resin complex, but surface treatment with EPS decreased transverse strength of PMMA resin complex (p<0.05). 2. Silane treated glass fiber increased Young's modulus of PMMA resin complex compared to desized glass fiber (p<0.05). 3. Roughness increased after abrasion test in case of PMMA resin reinforced with desized glass fiber (p<0.05). 4. Roughness change was not observed after abrasion test in case of PMMA resin reinforced with silane treated glass fiber (p>0.05).