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Cataloguing of Anther Expressed Genes through Differential Slot Blot in Oriental Lily (Lilium Oriental Hybrid 'Acapulco') (아카풀코나리에서 Differential Slot Blot을 이용한 약발현 유전자 목록작성)

  • Suh, Eun-Jung;Yu, Hee Ju;Han, Bong Hee;Lim, Yong Pyo;Jeong, Mi-Jeong;Lee, Seong-Kon;Kim, Dong-Hern;Chang, An-Cheol;Yae, Byeong Woo
    • Horticultural Science & Technology
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    • v.31 no.5
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    • pp.598-606
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    • 2013
  • Anther is the major organ of flower in responsible to reproduction and outward appearance. From anther-specific cDNA library of Lilium Oriental Hybrid 'Acapulco', 2000 expressed sequence tags were selected randomly. Differential slot blot analysis with cDNA probes from the anther and leaf was used to get anther-expressed clone and 570 non-redundant ESTs were obtained and sequenced. Compared to the GenBank database using BLASTX algorithm, 191 clones showed significant similarity but others (66.5%) did not measured to known sequence. Functional categories according to gene ontology (GO) annotation included sequence representing a significant portion of protein in cell and cell part respectively. A transcriptional analysis at 7 different organs and developmental stage was performed using northern blot with thirty ESTs as putative anther specific gene. This report suggest that selection of anther expressed clone using differential slot blot was considered as very effective tool and our current study can provide fundamental information on the lily anther including pollen furthermore.

Analysis of Experimental Researches in Korea on the Effects of Aromatherapy to Relieve Pain (아로마테라피의 통증 감소 효과에 관한 국내 실험논문 분석)

  • Park, Jeong-Sook;Park, Jeong-Eon;Yang, Jang-Soon;Kwak, Hye-Weon;Han, Jung-An
    • Journal of Hospice and Palliative Care
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    • v.14 no.1
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    • pp.8-19
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    • 2011
  • Purpose: This study identifies research trends and provides fundamental data related to curative power of aromatherapy to relieve pain in Korea. Methods: The study analyzed 44 experimental studies on humans that were published in Korea before the end of December, 2009. The key words used for searching were: aroma, aromatherapy, hyang-yobeob, hyanggi-yobeob, hyanggichilyo, aromatherapy and pain, headache, scapulodynia, omodynia, feeling uncomfortable in the perineal region, sense of pain, labor pains, arm pain, menstrual pain, aches, and dysmenorrheal. Those words were found on websites, including those for Korea Education & Research Information Service, the National Assembly Library, KISS, KoreaMed, and NDSL. Collected data were analyzed using descriptive statistics. Results: The results showed that most of essential oils to relieve pain is composed of three to four kinds of oils, including Lavender, Roman chamomile, Rosemary, and Clarysage. Aromatherapy is applied usually by means of massage (50%), inhalation (13.6%), or a combination of the above two (13.6%). Measuring instruments as a dependent variable include VAS, questionnaire, GRS, blood pressure, pulse, and Algometer. Aromatherapy plays an important role in soothing headaches and arthralgia. However, when it comes to labor and menstrual pain, it doesn't seem to be effective. Conclusion: The study found that different kinds of oil, frequencies, and periods of time are used for the same symptoms. Further research should employ standardized oil blending, application, duration, and measuring instruments, and more systematically analyze the effects of aromatherapy to establish the effects on relieving pain.

Analysis of Research Trends Related to Children's Department of Church School : Focusing on Domestic Dissertations (교회학교 유치부 관련 연구 동향 분석 : 국내 학위 논문 중심으로)

  • Kim, Minjung
    • Journal of Christian Education in Korea
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    • v.71
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    • pp.181-210
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    • 2022
  • The purpose of this study was to investigate the research trends related to the children's department of church schools. The purpose of this study is to present basic data for the study of the children's department of church schools by analyzing the research period, research contents, research methods, and subjects of research related to the children's department of church schools. For this study, 50 domestic master's and doctoral dissertations searched through the National Assembly Library and the Research Information Sharing Service(RISS) were extracted with the keywords of 'church school' and 'children's department'. The frequency and percentage were calculated by analyzing the research related to the children's department of the church school according to four criteria: research period, research content, research method, and research subject. As a result of the study, first, the research trend of research papers in the children's department of church schools was found to be 49 articles (98%) for master's degrees and 1 article (2%) for doctoral degrees from 1980 to 2022. Trends by research period are focused on master's degrees. Second, the trend by research content was 27 practical studies (54%) and 23 theory studies (46%). In the research related to the children's department of church schools, the practical research accounted for a relatively high percentage compared to the theory research. Third, the trends by research method were in the order of 30 literature studies (60%), 19 quantitative studies (38%), and 1 qualitative study (2%). Research related to children's departments in church schools is being actively conducted with a focus on literature research. Fourth, as for the trends by study subject, the study was conducted focusing on physical subjects, with 35 subjects (70%) and 15 subjects (30%) of personal subjects. As research is conducted from physical objects to church schools and media, it is necessary to study the connection between church schools and families. As the research on church school kindergarten is focused on adults (teachers, parents, and educational preachers), in-depth research on children in church schools and qualitative research with voices from the field of children's department in church schools are required.

Analysis of Research Trends Related to Christian Picture Books : Focusing on Domestic Dissertations (기독교 그림책 관련 연구 동향 분석 : 국내 학위 논문 중심으로)

  • Kim, Minjung
    • Journal of Christian Education in Korea
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    • v.68
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    • pp.245-277
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    • 2021
  • The purpose of this study was to investigate the trend of Christian picture book-related research. The purpose of this study is to present basic data for various and balanced research and development in the Christian picture book field by analyzing the research period, research content, and research method related to Christian picture books. For this study, 45 domestic master's and doctoral dissertations were extracted through the National Assembly Library and the Academic Research Information Service (RISS) with the keywords of 'Christian picture book', 'Bible picture book', 'Christian story', and 'Bible story'. The frequency and percentage were calculated by analyzing Christian picture book-related studies according to four criteria: research period, research content, research method, and research subject. As a result of the study, first, the trend of Christian picture book research papers by research period from 1999 to 2021 was 43 master's articles (95.6%) and 2 doctoral articles (4.4%), focusing on Christian picture book-related studies. Second, the trend by research content was found to be 12 basic studies (26.6%) and 33 practical studies (73.4%). Research related to Christian picture books is being actively conducted focusing on practical research rather than basic research. Third, the trend by research method was in the order of 33 quantitative studies (73.4%), 11 literature studies (24.4%), and 1 qualitative study (2.2%). Research related to Christian picture books is centered on quantitative research, and literature research and qualitative research account for a relatively low proportion. Fourth, as for the trends by study subject, there were 35 human subjects (77.8%) and 10 physical subjects (22.2%). Among human subjects, 33 single subjects (73.4%) and 2 mixed subjects (4.4%) were found, and among single subjects, 30 studies (66.7%) targeting children were high. In other words, research on Christian picture books had a higher proportion of studies with children as a single subject than mixed subjects between children and children, children and teachers, and between children and parents.

Analysis of Research Trends Related to Forest Play: Focusing on Domestic Dissertations (숲놀이 관련 연구 동향 분석: 국내 학위 논문 중심으로)

  • Kim, Minjung
    • Journal of Christian Education in Korea
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    • v.69
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    • pp.77-104
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    • 2022
  • The purpose of this study was to investigate the research trend of forest play. The purpose of this study is to provide basic data for the vitalization of forest play research by analyzing the research period, research content, and research methods. For this study, 57 domestic master's and doctoral dissertations were extracted through the National Assembly Library and the Research Information Sharing Service(RISS) with the keywords of 'forest', 'play', and 'forest play'. The frequency and percentage were calculated by analyzing forest play research based on four criteria: research period, research content, research method, and research subject. As a result of the research, first, the trend of forest play research by period is from 2011 to 2021, with 49 articles (85.9%) for master's degrees and 8 articles (14.1%) for doctor's degrees. Second, the trend by research content was found to be 16 basic studies (28.1%) and 41 practical studies (71.9%). Forest play research is being actively conducted centered on practical research. Third, the trends by research method were in the order of 39 quantitative studies (68.4%), 17 qualitative studies (29.8%), and 1 literature study (1.8%). Forest play research is focused on quantitative research, and comparatively qualitative research and literature research account for a low proportion. Fourth, the trend by study subject was 56 single subject studies (98.2%). The single subjects were 52 children (91.2%), 3 teachers (5.2%), and 1 parent (1.8%). As for the mixed subjects, there is one study (1.8%) targeting children and parents, and it is necessary to conduct a study with mixed subjects. As for the study of material subjects, 42 articles (73.7%) in the natural environment, 13 articles (22.8%) in educational institutions, and 2 articles (3.5%) in the media were found in the order. Research on the home environment related to forest play is insufficient, so research on parents, children-parents, and home environment related to forest play should be conducted in the future.