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http://dx.doi.org/10.7235/hort.2013.12225

Cataloguing of Anther Expressed Genes through Differential Slot Blot in Oriental Lily (Lilium Oriental Hybrid 'Acapulco')  

Suh, Eun-Jung (Molecular Breeding Division, National Academy of Agricultural Science, Rural Development Administration)
Yu, Hee Ju (Department of Life Science, Catholic University)
Han, Bong Hee (Department of Seed Service, The Foundation of Agriculture Technology Commercialization and Transfer)
Lim, Yong Pyo (Department of Horticulture, Chungnam National University)
Jeong, Mi-Jeong (Molecular Breeding Division, National Academy of Agricultural Science, Rural Development Administration)
Lee, Seong-Kon (Molecular Breeding Division, National Academy of Agricultural Science, Rural Development Administration)
Kim, Dong-Hern (Molecular Breeding Division, National Academy of Agricultural Science, Rural Development Administration)
Chang, An-Cheol (Molecular Breeding Division, National Academy of Agricultural Science, Rural Development Administration)
Yae, Byeong Woo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Publication Information
Horticultural Science & Technology / v.31, no.5, 2013 , pp. 598-606 More about this Journal
Abstract
Anther is the major organ of flower in responsible to reproduction and outward appearance. From anther-specific cDNA library of Lilium Oriental Hybrid 'Acapulco', 2000 expressed sequence tags were selected randomly. Differential slot blot analysis with cDNA probes from the anther and leaf was used to get anther-expressed clone and 570 non-redundant ESTs were obtained and sequenced. Compared to the GenBank database using BLASTX algorithm, 191 clones showed significant similarity but others (66.5%) did not measured to known sequence. Functional categories according to gene ontology (GO) annotation included sequence representing a significant portion of protein in cell and cell part respectively. A transcriptional analysis at 7 different organs and developmental stage was performed using northern blot with thirty ESTs as putative anther specific gene. This report suggest that selection of anther expressed clone using differential slot blot was considered as very effective tool and our current study can provide fundamental information on the lily anther including pollen furthermore.
Keywords
anther; ESTs (expressed sequence tags);
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