• Asian-Australasian Journal of Animal Sciences
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• 제25권4호
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• pp.559-568
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• 2012

White (Lohmann LSL) and Brown (ATAK-S) laying hens, were reared under organic and conventional cage rearing systems, and the effects of the rearing system on performance parameters, egg production, egg characteristics, and immune response were investigated. For this purpose, a total of 832 laying hens of two commercial hybrids, i.e., 416 white (Lohmann LSL) and 416 Brown (ATAK-S) layers, were used. The experiment lasted between 23 and 70 wk of age. In this study, the white layers yielded more eggs as compared to the brown layers in both organic and conventional production systems. Egg weight exhibited a similar pattern to that of laying performance. However, the total hen-housed egg number for the white birds in the organic system was fewer than that of white birds in the conventional cage facility; conversely, a contradictory tendency was observed for the brown birds. Livability of the white layers in the organic system was remarkably lower (14%) than that of the brown line, whereas the white line survived better (3.42%) than their brown counterparts in conventional cages. The feed conversion ratio of the white hens was markedly inferior in the organic system as compared to that of the white hens in the conventional system, whereas relatively lower deterioration was reported in brown layers when reared in an organic system. The organic production system increased egg albumen height and the Haugh unit in eggs of the brown layers. The yolk color score of organic eggs was lower than that of conventional eggs for both brown and white hens. The egg yolk ratio of eggs from white layers was found to be higher in organic eggs as compared to those obtained in the conventional system. All organic eggs had heavier shells than those produced in the conventional system. Eggs from brown layers had more protein content than eggs from white layers. Neither housing systems nor genotype influenced egg yolk cholesterol concentration. When compared to conventional eggs, n-3 fatty acid content was lower in organic eggs, and the n-6:n-3 ratio was higher in organic eggs. In conclusion, two hen genotypes showed different responses in terms of performance and egg quality to two different rearing systems. A commercial white strain produced more eggs with higher egg quality as compared to a native brown strain. The brown strain was found to have adapted well to organic production conditions when survival and total egg number was taken into consideration.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.5635-5641
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• 2015

Background: Cirrhosis is regarded as a possible end stage of many liver diseases, including viral infection. It occurs when healthy liver tissue becomes damaged and is replaced by scar tissue and finally may lead to hepatocellular carcinoma. Interferons (IFNs)are two general categories, type I and II. Type I includes one beta interferon and over 20 different alpha interferons. Alpha interferons are very similar in how they work, interacting with other proteins on cells like receptors. The main objective of this study was to compare Mx gene productivity post different cell line treatment with imported and Egyptian biosimilar locally produced IFNs, as well as the efficacy of those tested IFNs. Also, an assessment was made of sensitivity of different cell lines as alternatives to that recommended for evaluation of antiviral activity. Materials and Methods: Different cell lines (Vero, MDBK and Wish) were employed to evaluate cytotoxicity using the MTT assay. Antiviral activity was evaluated compared with standard IFN against VSV, Indiana strain -156, on tested rh-IFNs (imported; innovated and Egyptian biosimilar locally produced IFNs) in the pre-treated cell lines previously mentioned. The virus was propagated in the Wish cell line as recommended. Finally we estimated up-regulation of the Mx gene as a biomarker. Results: Data recorded revealed that test IFNs were safe in test cell lines. Viability was around 100%. Locally tested interferon did not realize the international potency limits, while the imported one was accepted compared with the standard IFN. These results were the same either using infectivity titer reduction assay or crystal violet staining of residual non- infected cells. Mx protein production was cell type related and confirmed by the detected Mx gene expressed in imported and locally produced IFN pre-treated cell lines. The expression of the gene was arranged in the order of Vero> wish > MDBK for the imported IFN, while for the Egyptian biosimillar locally produced one it was MDBK> Vero> wish. With regard to the antiviral activity there was a significant difference of imported IFN potency compared with the locally produced IFN (P<0.05), the IFN potential (antiviral activity) was not cell line related and showed non-significant difference for each separate product. Conclusions: Vero cells can be used as an alternative cell line for evaluation of IFN potency in case of unavailable USP recommended cell lines. Alternative potency evaluation assay could be used and proved significant difference in IFN potency in case of local and imported agents. Evaluation of antiviral activity could be used in parallel to viral infectivity reduction assay for better accuracy. Mx gene can be used as a marker for IFN potential.

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3519-3528
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• 2012

Inhibitory effects of Maclura amboinenesis Bl, one plant used traditionally for the treatment of cancers, on metastatic potential of highly metastatic B16F10 melanoma cells were investigated in vitro. Cell proliferation was assessed using the MTT colorimetric assay. Details of metastatic capabilities including invasion, migration and adhesion of B16F10 melanoma cells were examined by Boyden Chamber invasion and migration, scratch motility and cell attachment assays, respectively. The results demonstrated that n-hexane and chloroform extracts exhibited potent anti-proliferative effects (p<0.01), whereas the methanol and aqueous extracts had less pronounced effects after 24 h exposure. Bioactivity-guided chromatographic fractionation of both active n-hexane and chloroform extracts led to the isolation of two main prenylated xanthones and characterization as macluraxanthone and gerontoxanthone-I, respectively, their structures being identified by comparison with the spectral data. Interestingly, both exhibited potent effective effects. At non-toxic effective doses, n-hexane and chloroform extracts (10 and $30{\mu}g/ml$) as well as macluraxanthone and gerontoxanthone-I (3 and $10{\mu}M$) significantly inhibited B16F10 cell invasion, to a greater extent than $10{\mu}m$ doxorubicin, while reducing migration of cancer cells without cellular cytotoxicity. Moreover, exposure of B16F10 melanoma cells to high concentrations of chloroform ($30{\mu}g/ml$) and geratoxanthone-I ($20{\mu}M$) for 24 h resulted in delayed adhesion and retarded colonization. As insights into mechanisms of action, typical morphological changes of apoptotic cells e.g. membrane blebbing, chromatin condensation, nuclear fragmentation, apoptotic bodies and loss of adhesion as well as cell cycle arrest in the G1 phase with increase of sub-G1 cell proportions, detected by Hoechst 33342 staining and flow cytometry were observed, suggesting DNA damage and subsequent apoptotic cell death. Taken together, our findings indicate for the first time that active n-hexane and chloroform extracts as well as macluraxanthone and gerontoxanthone-I isolated from Maclura amboinensis Bl. roots affect multistep of cancer metastasis processes including proliferation, adhesion, invasion and migration, possibly through induction of apoptosis of highly metastatic B16F10 melanoma cells. Based on these data, M. amboinensis Bl. represents a potential candidate novel chemopreventive and/or chemotherapeutic agent. Additionally, they also support its ethno-medicinal usage for cancer prevention and/or chemotherapy.

• Asian-Australasian Journal of Animal Sciences
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• 제17권5호
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• pp.670-677
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• 2004

Sixteen multiparous Holstein-Friesian crossbred cows in mid-lactation were blocked according to days in milk (DIM) and previous lactation and randomly assigned according to a Randomized Complete Block (RCB) design with four replications to receive four dietary treatments. The dietary treatments consisted of T1: No cassava hay (CH) or stylo 184 hay (SH) supplementation, supplementation of concentrate to milk yield at 1:2 (control), T2: Supplementation of 1 kg of CH/hd/d, supplementation of concentrate to milk yield at 1:2, T3: Supplementation of 1 kg of CH+SH/hd/d, supplementation of concentrate to milk yield at 1:2, T4: Supplementation of 2 kg of CH+SH/hd/d, supplementation of concentrate to milk yield at 1:3. All animals received Ruzi grass from a cut-and-carry system as roughage source. The feeding trial lasted for 9 weeks. The results revealed that DMI of concentrate of supplemented treatments were significantly lower (p<0.05) than those in the control, but there was no significant difference between T2 and T3. There was no significant difference in forage DM intake between the control and supplemented treatments. CP and NDF digestibility of supplemented treatments were significantly (p<0.05) greater than the control and there were no significant differences among supplemented treatments. Milk yield and 3.5% FCM (14.3, 14.5, 14.7 and 14.8; 13.9, 14.3, 14.3 and 14.6 kg/hd/d, respectively) were not significantly different among treatments. Milk protein percentage of supplemented treatments was significantly (p<0.05) higher than the control, but there were no significant differences among supplemented treatments. There was no significant difference in milk fat percentage between the control and supplemented treatments. However, milk fat percentage tended to be higher for supplemented animals as compared to the control group. There were also no significant differences in lactose, solids-not-fat and total solids percentages among treatments. Cows in supplemented treatments gave incomes over supplement cost (IOSC) of 2.72, 2.74 and 2.93 US$/hd/d, respectively which were greater than for cows on control treatment. Furthermore, IOSC were greatest for cows in T4 as compared to other treatments. Based on this study it was concluded that, feeding cassava hay solely or in combination with stylo 184 hay as a supplemental protein source could be a potential valuable strategy in small-holder dairy farming systems in the tropics. This strategic supplementation significantly reduced concentrate use, which resulted in improved milk yields and milk quality for the supplemented cows. Moreover, it resulted in higher economical returns through increased productivity and lower ratios of concentrate to milk yield, from 1:2 to 1:3.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.3357-3362
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• 2013

The human skeleton is the most common organ to be affected by metastatic cancer and bone metastases are a major cause of cancer morbidity. The five most frequent cancers in Malaysia among males includes prostate whereas breast cancer is among those in females, both being associated with skeletal lesions. Bone metastases weaken bone structure, causing a range of symptoms and complications thus developing skeletal-related events (SRE). Patients with SRE may require palliative radiotherapy or surgery to bone for pain, having hypercalcaemia, pathologic fractures, and spinal cord compression. These complications contribute to a decline in patient healthrelated quality of life. The multidimensional assessment of health-related quality of life for those patients is important other than considering a beneficial treatment impact on patient survival, since the side effects of treatment and disease symptoms can significantly impact health-related quality of life. Cancer treatment could contribute to significant financial implications for the healthcare system. Therefore, it is essential to assess the health-related quality of life and treatment cost, among prostate and breast cancer patients in countries like Malaysia to rationalized cost-effective way for budget allocation or utilization of health care resources, hence helping in providing more personalized treatment for cancer patients.

• 한국수산과학회지
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• 제7권4호
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• pp.195-203
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• 1974

제주도산 은어의 어체량이 전소한 사실에 대하여 그 원인을 구명하는 일환으로 성장이 빠른 하계의 개체생산 속도를 1973년 6월 18일부터 9월 25일까지 강정천에서 조사한 바 자료수가 만족스럽지는 못하나 본체조자료에서는 다음과 같은 결과를 추정할 수 있었다. 1) 1973년도 하계에 있어서 강정천산 은어의 개체생산속도에 대하여 Table 5와 같이 추정하였다. 2) 강정천산 은어의 개체생산속도는 일본 우주산의 것게 비하여 불과 $7.2\%$였고, 부착조류에 현존량도 $6.7\%$에 지나지 않으며 전체적으로 극히 미비하였다. 3) 조사기간을 통하여 부착조류의 현존량으로 볼 때 서식밀도가 평균 5.2미/$m^2$로써 수용한계추정 미수 4.8미 /$m^2$보다 많은 수인 5.2미/$m^2$였다. 4) 강정천산 은어의 하계 개체생산속도는 주로 8월에서 9월 사이에 왕성히 이루어지고 있음을 알았다. 5) 개체생산속도의 성적이 좋지 못한 것은 이식량의 부족에 있는 것으로 보았으며, 이는 부착조류의 생산량이 적고 서식밀도가 과다한 데다 일중 평균수온이 낮아 섭이활동이 활발치 못한 데서 기인한 것으로 추측되었으며, 이러한 요인들이 일차적으로 강정천의 은어 생산속도에 크게 영향을 미치고 있는 것으로 보았다.

• 한국수산과학회지
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• 제24권1호
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• pp.1-8
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• 1991

문치가자미는 동경만의 소형저인강 어업의 중요한 대상종이나 최근 어획노력이 증가하는 경향이 있어 자원을 영속적으로 유효하게 이용하기 위하여 자원 평가를 하였다. 복수년급군에 대한 VPA 해석 결과, 1980년부터 1987년까지의 자원미수는 약 350만 마리에서 920만 마리였고, 1985년에 최대치를 보였다. 시험조업에 의한 추정량은 240만에서 870만 마리였고 최대치를 보인 해도 같았다. 양자의 차는 1987연에 약 2배였으나 그 밖의 해에는 0.8-l.5배의 차로 그다지 큰 차를 보이지 않았다. 양쪽 다 어획량과 단위노력당 어획양의 증감 경향이 유사했다 어획률은 대부분의 해에 $30-50\%$로 상당히 높은 수치를 보였다. COHORT 해석을 하여 어획계수를 추정하고, 수명으로 추정한 자연사망계수와 어획물과 성장에 의해 검토한 어획개시연령을 이용하여 등어획량곡선을 그려 자원현상을 평가하고 어획증대의 가능성을 검토한 바 가입당 어획량의 최대치가 암컷은 2배 숫컷은 1.3배 정도 증대 가능성을 보였다. 어획증대를 위해서는 강목을 크게 하여, 어획개시년령을 높이는 관리방안이 바람직하다.

• 한국수산과학회지
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• 제44권3호
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• pp.232-236
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• 2011

A feeding trial was carried out to investigate the effect of different fish meal and wheat flour contents in extruded pellets on the growth and apparent nutrient digestibility of olive flounder Paralichthys olivaceus. Four extruded pellets (HB1, HB1T, HB2 and HB2T) were prepared using two kinds of high quality brown fish meal and low quality tuna fish meal. Two diets (HB1 and HB2) contained two different kinds of brown fish meal, respectively; in HB1T and HB2T, we substituted 50 % of the brown fish meal with 10 % wheat flour. Three replicate groups of fish (initial body weight of 82 g) were fed the experimental pellets to visual satiation for 15 weeks. The highest weight gain was observed in fish fed HB1, and weight gain of fish fed HB2 was significantly higher than that of fish fed HB2T (P<0.05). Feeding efficiency for fish fed HB1 was significantly higher than that of fish fed HB1T and HB2T (P<0.05), but was not significantly different from fish fed HB2. Apparent dry matter digestibility in HB1 and HB2 was significantly higher than in HB1T and HB2T (P<0.05). The highest crude protein digestibility was observed in HB1, and protein digestibility in HB1T and HB2 was higher than in HB2T (P<0.05). The results of this study suggest that fish meal quality considerably affects growth and nutrient digestibility of olive flounder fed extruded pellets, and the use of HB1 is recommended over HB1T, HB2 and HB2T for efficient olive flounder production.

• 한국유통학회지:유통연구
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• 제16권5호
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• pp.211-231
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• 2011

상업론의 전통을 이어받은 일본 마케팅학계의 선구자들은 과점(寡占)메이커가 구축한 유통계열화에 대해 중소상인을 배급자로 전락시킨다고 하여 비판적이었고, 멀지 않은 장래에 붕괴될 것으로 예견했다. 한편 이러한 규범론적 연구그룹에 대해 거의 모든 소비재업계에 보편적으로 존재하는 유통계열화야 말로 미국식 마케팅 전략의 진수라고 평가하는 실증주의 연구그룹도 존재했다. 일본의 유통경로연구는 이러한 전통적인 규범주의 연구와 미국의 영향을 받은 경험주의 연구의 건전한 대립에 의해 발전하였다. 특기해야 할 것은 대형소매기업의 대두가 본격화되는 1980년대에 들어와 유통계열화가 약체화하기 시작했다는 점과 그 반면에 오랫동안 대립관계에 있었던 과점메이커와 대형소매기업간의 파트너십관계가 진전되기 시작했다는 점이다. 이에 따라 일본의 유통경로연구의 입장은 크게 변했다. 유통계열화라는 연구대상이 소멸했다고 판단했기 때문에 종래 이를 비판하고 상인옹호론을 펼쳤던 규범적 연구자들이 대형소매기업이 주도권을 가지는 제판동맹(製販同盟)을 긍정적으로 받아들인 것이다. 한편 유통계열화의 순기능을 지지했던 경험적 연구자들도 태도를 바꾸었다. 실증주의의 가르침에 따라 제판동맹이라는 새로운 실천현상을 연구의 프론티어로 인식한 것이다. 이처럼 일본의 유통경로연구가 일제히 유통계열화에서 제판동맹으로 연구의 중점을 이동시키면서, 방법론적으로 규범주의를 옹호하는 연구자는 그 모습을 감추고 다투어 경험주의 연구에 치중하게 된다. 일본적 마케팅의 주역으로서 규범주의적 색채를 남겼던 유통경로연구 조차도 방법론적으로 전향하게 된 것이다. 본고는 유통계열화의 종언을 전제로 하는 이러한 일본유통경로연구의 움직임에 대해 비판적인 견해를 피력한다.

• 한국산림과학회지
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• 제97권6호
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• pp.576-581
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• 2008

산지별 물푸레나무 종자의 산지이동시 생태적 조건에 대한 적응력을 예측하고 직파조림 및 포지양묘 시 최대의 발아효과를 얻고자 $5{\sim}35^{\circ}C$의 범위에서 온도에 대한 발아반응을 조사한 결과, 발아율, 발아속도에서 채취산지간 차이가 관찰되었다. 인제에서 채취한 종자가 저온($5{\sim}15^{\circ}C$)에서 발아율이 우세하였으며, 고온($30{\sim}35^{\circ}C$)에서는 강릉의 종자가 우세하였다. 또한 발아율 값에 의한 2차 회귀식 모델에서 도출된 기준온도, 최대온도 및 적정온도는 4개 산지간 다양하게 나타났는데, 인제가 가장 낮았고 강릉이 가장 높게 나타났다. 발아속도에 근거한 주요 온도 추정 모델 역시 산지간에 다양하게 나타났다. 기준온도의 경우 횡성이 가장 낮았으며 강릉이 가장 높았다. 최대온도 및 적정온도는 인제에서 가장 낮게 나타났으며, 역시 강릉에서 가장 높게 나타나 발아에 영향을 미치는 주요 온도들의 경우 저온산지보다 비교적 고온인 산지에서 더 높게 나타남을 알 수 있었다. 유묘생산을 위한 파종시 주요 온도를 고려할 때 2차 회귀모델과 선형모델 중 어느 것을 선택할 지는 유묘생산시의 목표에 달려있다. 즉 유묘생산량에 초점을 두었다면 2차 회귀모델을, 출현속도나 균일성에 초점을 두었다면 선형모델을 선택함이 바람직할 것이다.