• 전기학회논문지P
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• 제59권4호
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• pp.345-350
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• 2010

This paper presents a Photovoltaic Generation system using a Space Vector Modulation. PWM voltage source inverter using inverter consists of complex type of electric power converter to compensate for the defect, that is solar cell cannot be developed continuously by connecting with the source of electric power for ordinary use. It can cause the effect of saving electric power, from 10 to 20[%]. Synchronous signal and control signal was processed by the 56F8323 microprocessor for stable modulation. Also, Waveforms output current and voltage of system controlled so that phase conforms and can supply electric power that stabilize by the unit power factor.

• 대한수학회지
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• 제58권1호
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• pp.109-122
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• 2021

In this paper, we consider the problem of finding the hypersurface Mn in the Euclidean (n + 1)-space ℝn+1 that satisfies an equation of mean curvature type, called singular minimal hypersurface equation. Such an equation physically characterizes the surfaces in the upper half-space ℝ+3 (u) with lowest gravity center, for a fixed unit vector u ∈ ℝ3. We first state that a singular minimal cylinder Mn in ℝn+1 is either a hyperplane or a α-catenary cylinder. It is also shown that this result remains true when Mn is a translation hypersurface and u is a horizantal vector. As a further application, we prove that a singular minimal translation graph in ℝ3 of the form z = f(x) + g(y + cx), c ∈ ℝ - {0}, with respect to a certain horizantal vector u is either a plane or a α-catenary cylinder.

• Journal of Applied Biological Chemistry
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• 제53권1호
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• pp.1-7
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• 2010

xylA 프로모터는 대장균의 xylose 대사에 관여하는 xylose 오페론 상의 중요한 프로모터이다. 이 프로모터는 xylose에 의해 강하게 조절을 받는다고 알려져 있다. 이러한 특징은 새로운 발현 백터를 구축하는데 충분한 조건을 갖추고 있다고 생각된다. 본 연구에서는 이러한 xylose에 의해 유도 되는 발현벡터를 구축하기 위하여 600 bp의 xylA 프로모터를 증폭하여 pUC18의 AatII와 HindIII 사이에 삽입하여 pXA600을 구축하였다. 또한 조절단백질인 XylR의 영향을 조사하기 위하여 xylR 유전자를 삽입하여 pXAR600을 구축하였다. 발현의 강도를 측정하기 위하여 3,048 bp의 lacZ유전자를 xylA 프로모터의 하류에 연결하여 pXA600-lacZ와 pXAR600-lacZ를 구축하고 대장균 JM109에 형질전환시켰다. 구축된 pXA600-lacZ와 pXAR600-lacZ는 LB 배지에서 배양하였을 때 xylose 유도하에서 각각 1,641 unit와 2,304 unit의 $\beta$-galactosidase 활성을 보였으며, DM 배지상에서 배양했을 때 xylose 유도 시 각각 6,282 unit와 9,320 unit의 $\beta$-galactosidase 활성을 보였다. 또한 왜래 유전자의 발현 가능성을 확인하기 위하여 S. thermocyaneoviolaceus의 내열성 xylanase를 코딩하는 xynA 유전자를 실제로 구축된 pXA600과 pXAR600에서 발현을 확인하여 pXA600 및 pXAR600이 새로운 xylose 유도성 발현벡터로서의 사용 가능성을 확인하였다.

• 스마트미디어저널
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• 제8권2호
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• pp.74-79
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• 2019

기존의 복합명사 분해 알고리즘은 미등록어 단위명사들이 포함된 복합명사를 분해할 때 미등록어를 분리하기 어려운 문제가 발생한다. 이는 현실적으로 모든 고유명사, 신조어, 외래어 등의 모든 단위 명사를 사전에 등록하는 것은 불가능하다는 한계가 존재하기 때문이다. 이 문제를 해결하기 위하여 복합명사 분해 문제를 태그 열 부착(sequence labeling) 문제로 정의하고 음절 단위 임베딩과 딥러닝 기법을 이용하는 복합명사 분해 방법을 제안한다. 단위명사 사전을 구축하지 않고 미등록 단위명사를 인식하기 위하여 복합명사를 구성하는 각 음절들을 연속적인 벡터 공간에 표현하여 LSTM과 선형체인(linear-chain) CRF를 이용하는 방식으로 복합명사를 단위명사들로 분해한다.

• 전자공학회논문지
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• 제53권11호
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• pp.41-47
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• 2016

고효율 영상 부호화 기술인 high efficiency video coding (HEVC)은 부호화 효율을 높이기 위하여 coding tree unit (CTU)을 사용한다. CTU는 coding unit (CU), prediction unit (PU), transform unit (TU)으로 구성되며 모든 가능한 경우의 CU, PU, TU 분할연산을 통해 최적의 분할 조합을 찾아내게 된다. 블록 분할 연산의 복잡도를 감소시키기 위하여 본 논문은 움직임 벡터에 의한 관심 영역 CTU 추출에 근거하는 PU 분할 결정 방법과 이전에 부호화된 프레임의 같은 위치의 CTU 정보를 사용하는 CU 깊이 결정 분할 알고리즘을 제안한다. 첫 번째 방법은 프레임 중 움직임이 많은 동적 CTU 부분과 움직임이 적은 정적 CTU 부분으로 나누어 정적인 영역에 대해 PU 분할 연산을 감소시키는 방법이며, 두 번째 방법은 이전 프레임의 CTU 깊이 정보를 기반으로 현재 CTU의 분할 깊이를 미리 예측하여 CU 분할 연산을 감소시킨다. 결과적으로 제안하는 알고리즘은 HEVC test model (HM) 14.0 버전 대비 BDBR 손실은 2.5% 발생했지만, 전체 부호화 시간이 약 44.8%로 크게 감소했다.

• Journal of Microbiology and Biotechnology
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• 제29권10호
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• pp.1644-1655
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• 2019

Saccharomyces cerevisiae (S. cerevisiae) and glucagon-like peptide-2 (GLP-2) have been employed to improve the intestinal development of weaned animals. The goal of this study was to determine whether either exogenous S. cerevisiae or GLP-2 elicits major effects on fecal microbiotas and cytokine responses in weaned piglets. Ninety-six piglets weaned at 26 days were assigned to one of four groups: 1) Basal diet (Control), 2) empty vector-harboring S. cerevisiae (EV-SC), 3) GLP-2-expressing S. cerevisiae (GLP2-SC), and 4) recombinant human GLP-2 (rh-GLP2). At the start of the post-weaning period (day 0), and at day 28, fecal samples were collected to assess the bacterial communities via sequencing the V1-V2 region of the 16S-rRNA gene, and piglets' blood was also sampled to measure cytokine responses (i.e., IL-$1{\beta}$, TNF-${\alpha}$, and IFN-${\gamma}$). This study revealed that, on the one hand, although S. cerevisiae supplementation did not significantly alter the growth of weaned piglets, it induced increases in the relative abundances of two core genera (Ruminococcaceae_norank and Erysipelotrichaceae_norank) and decreases in the relative abundances of two other core genera (Lachnospiraceae_norank and Clostridiale_norank) and cytokine levels (IL-$1{\beta}$ and TNF-${\alpha}$) (p < 0.05, Control vs EV-SC; p < 0.05, rh-GLP2 vs GLP2-SC). On the other hand, GLP-2 supplementation had no significant influence on fecal bacterial communities and cytokine levels, but it produced better body weight and average daily gain (p < 0.05, Control vs EV-SC; p < 0.05, rh-GLP2 vs GLP2-SC). Therefore, altered fecal microbiotas and cytokine response effects in weaned piglets were due to S. cerevisiae rather than GLP-2.

• 약학회지
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• 제49권3호
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• pp.217-224
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• 2005

Human Immunodeficiency Virus type 1 (HIV-l) based lentivirus vector has demonstrated great potential as gene therapy vectors mediating efficient gene delivery and long-term transgene expression in both dividing and nondividing cells. However, for clinical studies it must be confirmed that vector preparations are safe and not contaminated by replication competent lentivirus (RCL) related to the parental pathogenic virus, HIV-l. In this study, we would like to establish the method for titration and RCL detection of lentivirus vector. The titration was determined by vector expression containing the green fluorescent protein, GFP in transduced cells. The titer was $1{\times}10^7$ Transducing Unit/ml in the GFP expression assay and $8.9{\times}10^7$ molecules/ml in the real-time PCR. Also, for the detection of RCL, we have used a combination method of PCR and p24 antigen detection. First, PBS/psi and VSV-G region in the genomic DNA of transduced cells was detected by PCR assay. Second, transfer and expression of the HIV-1 gag gene was detected by p24 ELISA. In an attempt to amplify any RCL, the transduced cells were cultured for 3 weeks (amplification phase) and the supernatant of amplified transduced cell was used for the second transduction to determine whether a true RCL was present (indicator phase). Analysis of cells and supernatant at day 6 in indicator phase were negative for PBS/psi, VSV-G, and p24 antigen. These results suggest that they are not mobilized and therefore there are no RCL in amplification phase. Thus, real-time PCR is a reliable and sensitive method for titration and RCL detection of lentivirus vector.

• Journal of Power Electronics
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• 제15권3호
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• pp.670-684
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• 2015

The hybrid cascaded multilevel inverter (HCMLI) is a popular converter topology that is being increasingly used in high power medium voltage drives. The intricacy of the control technique for a HCMLI increases with the number of levels and due to fluctuating dc voltages. This paper presents a novel offline quadrant search based space vector modulation technique to synthesize a sinusoidal output from a dispersed pattern of voltage vectors due to different voltages in the auxiliary unit. Such an investigation has never been reported in the literature and it is being attempted for the first time. The method suggested distributes the voltage vectors for a reduced total harmonic distortion at minimal computation. In addition, the proposed algorithm determines the maximum modulation index in the linear modulation range in order to synthesize a sinusoidal output for both normal and abnormal vector patterns. It is better suited for a wide range of practical applications. It is particularly well suited for renewable source fed inverters which utilize large capacitor banks to maintain the dc link, which are prone to such slow fluctuations. The proposed quadrant search space vector modulation technique is simulated using MATLAB/SIMULINK and implemented using a Nexys-2 Spartan-3E FPGA for a developed prototype.

• Biomolecules & Therapeutics
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• 제13권3호
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• pp.143-149
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• 2005

We have demonstrated previously on a replication incompetent recombinant adenoviral vector, AdLPCD, in which the expression of cytosine deaminase (CD) gene is driven by the tumor-specific L-plastin promoter. The object of this study was to evaluate the efficacy of AdLPCD together with 5-fluorocytosine (5-FC) in suppression of the growth of established human tumor cells of ovary, Consistent with the knowledge that infection of OVCAR-3 cells with AdLPCD resulted in expression of a functional intracellular CD enzyme capable of converting 5-FC to 5-fluorouracil (5-FU) (Chung and Deisseroth, 2004), statistically significant differences in cytotoxicity were observed when AdLPCD infected cells were also exposed to 5-FC for 6 days (p=0.05), 9 days (p<0.0005) and 12 days (p<0.005), compared to 5-FC exposure alone, These results indicate that the CD gene delivered by adenoviral vector could efficiently sensitize OVCAR-3, otherwise non-toxic 5-FC. On the other hand, SKOV-3 cells, an ovarian carcinoma cell line, were more resistant to the CD/5-FC strategy compared with OVCAR-3 cells under the same condition. The results of present study suggest that the replacement of 5-FU with CD/5-FC in combination chemotherapy would be less toxic and much greater cytotoxicity than the conventional combination chemotherapy in some patients.

• Journal of Microbiology and Biotechnology
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• 제2권3호
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• pp.174-182
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• 1992

The hemagglutinin-esterase glycoprotein (HE) gene of bovine coronavirus, coupled with a simian virus 40 early promoter and polyadenylation signal, was inserted into a human adenovirus transfer vector. The transfer vector was used to co-transfect 293 cells along with adenovirus genomic DNA. The hemagglutinin-esterase transcription unit was rescued into the adenovirus genome by homologous in vivo DNA recombination between the vector plasmid DNA and the adenovirus genomic DNA, and a recombinant adenovirus was isolated by several rounds of plaque assays. Thus the recombinant adenovirus carries the hemagglutinin-esterase gene in the early transcription region 3 (E3) of the adenovirus genome in the parallel orientation to the E3 transcription. The recombinant adenovirus synthesized the HE polypeptide in HeLa cells as demonstrated by immunoprecipitation with anti-coronavirus rabbit antisera. The recombinant HE polypeptide could be labelled by $[^3H]$glucosamine, demonstrating that the recombinant HE was glycosylated. Cells expressing the HE polypeptide exhibited hemadsorption activity when incubated with mouse erythrocytes. The HE was transported to the plasma membrane as shown by the cell surface immunofluorescence, indicating that the recombinant HE polypeptide retained its biological activities. Potential for the use of infectious recombinant adenovirus as a live virus-vectored vaccine candidate for bovine coronavirus disease is discussed.