• Korean Journal of Mathematics
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• 제8권2호
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• pp.155-162
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• 2000

For a sequence $\{{\eta}_m\}_m$ of unit vectors in $\mathbb{C}^n$, we consider the associated linear functional ${\omega}$ on the Cuntz algebra $\mathcal{O}_n$. We show that the restriction ${\omega}{\mid}_{UHF_n}$ is the product pure state of a subalgebra $UHF_n$ of $\mathcal{O}_n$ such that ${\omega}{\mid}_{UHF_n}={\otimes}{\omega}_m$ with ${\omega}_m({\cdot})$ < ${\cdot}{\eta}_m,{\eta}_m$ >. We study product pure states of UHF and obtain a concrete description of them in terms of unit vectors. We also study states of $UHF_n$ which is the restriction of the linear functionals on $O_n$ associated to a fixed unit vector in $\mathbb{C}^n$.

• 호남수학학술지
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• 제36권4호
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• pp.805-812
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• 2014

For unit tangent sphere bundles $T_1M$ with the standard contact metric structure (${\eta},\bar{g},{\phi},{\xi}$), we have two fundamental operators that is, $h=\frac{1}{2}{\pounds}_{\xi}{\phi}$ and ${\ell}=\bar{R}({\cdot},{\xi}){\xi}$, where ${\pounds}_{\xi}$ denotes Lie differentiation for the Reeb vector field ${\xi}$ and $\bar{R}$ denotes the Riemmannian curvature tensor of $T_1M$. In this paper, we study the Reeb ow invariancy of the corresponding (0, 2)-tensor fields H and L of h and ${\ell}$, respectively.

• 한국미생물·생명공학회지
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• 제26권1호
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• pp.68-75
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• 1998

A. niger의 GOD(Glucose Oxidase) 대량생산과 효율적인 분비를 protein의 대량생산에 많이 사용되는 strain인 S. cerevisiae에서 시도하였다. S. cerevisiae의 ADH1과 GAL 10 promotor, 그리고 ${alpha}$-MF signal sequence와 A. oryzae의 ${alpha}$-amylase signal sequence 및 S. cerevisiae의 GAL7과 A. niger의 GOD terminator를 이용하여 4개의 expression vector를 합성한 후 S. cerevisiae 2805에 auxotroph 방법으로 형질변환시켰다. 변이체들을 배양하여 세포내와 세포외의 GOD활성도를 분석한 결과 GAL 10 promotor가 삽입된 pGAL변이체들이 ADH1 promotor가 삽입된 pADH 변이체들 보다 GOD 생산성이 높았다. GAL 10 promotor와 A. oryzae의 ${alpha}$-amylase signal sequence가 삽입된 pGALGO2에서 115시간 배양시 GOD의 생산이 가장 높았다($GOD_{total}$: 10.3 unit/mL, $GOD_{ex}$: 8.7 unit/mL). 이 수치는 같은 promotor인 GAL 10 promotor와 ${alpha}$-MF signal sequence가 삽입된 pGALGO1보다 3배정도 높다. 이 결과는 ADH 1 promotor를 사용하였을 경우에도 일치하였다. 또한 A. oryzae의 ${alpha}$-amylase signal sequence가 S. cerevisiae의 ${alpha}$-MF signal sequence보다 GOD를 더 효과적으로 분비시켰다. 상기 결과로 미루어 보면 signal sequence가 단백질의 분비 외에도 단백질 합성에도 많은 영향을 주는 것으로 추측된다. pGALGO1과 pGALGO2의 GOD분비효율은 각각 89%, 84%이었다. S. cerevisiae에서는 일반적으로 과당화가 일어나기 때문에 S. cerevisiae에서 합성된 재조합 GOD의 분자량은 250 kDa으로 A. niger의 GOD(170 kDa)보다 더 컸다.

• Journal of Microbiology and Biotechnology
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• 제8권1호
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• pp.42-48
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• 1998

In order to maximize the secretory expression of human serum albumin (HSA) in the yeast Saccharomyces cerevisiae, a series of HSA expression vectors were constructed with a combination of different promoters, 5' untranslated regions (5'UTR), and secretion signal sequences. The expression vector composed of the galactose-inducible promoter GALl0, the natural 5'UTR, and the natural signal sequence of HSA directed the most efficient expression and secretion of HSA among the constructed vectors when introduced into several S. cerevisiae strains. Although the major form of HSA expressed and secreted in the yeast transformants was the mature form of 66 kDa, the truncated form of 45 kDa was also detected both in the cell extract and in the culture supernatant. The level of the intact HSA protein in the culture supernatant reached up to 30 mg/l at 24 h of cultivation in a shake-flask culture but began to decrease afterwards, indicating that the secreted HSA protein was unstable in a prolonged culture of yeast.

• 한국자동차공학회논문집
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• 제11권5호
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• pp.170-175
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• 2003

A control algorithm is developed for highly efficient operation of auxiliary power unit (APU) that consists of a diesel engine and a directly coupled induction generator in series hybrid electric Bus (SHEB). In a series hybrid configuration the APU supplies the electric power needed for maintaining the state of charge (SOC) of the battery unit in various conditions of vehicle operation. As the rotational speed of generator does not depend on the vehicle speed, an optimized operation of engine-generator unit based on the efficiency map of each component can be achieved. The output torque of diesel engine can be controlled by the amount of fuel injection, and the power converted from mechanical to electrical energy can be adjusted by generate control unit (GCU) using the decoupling vector control of torque and flux. As for the given reference of the generating power, the multiply of speed and torque, many combinations of operating speed and torque are possible. The algorithm decides the new operating point based on the engine efficiency map and generator characteristic curve. During the transition of operating points, the speed controller saturation is avoided using variable limit and filtering of generator torque reference. A test rig and SHEB consist of a 1.5L diesel engine and a 30kw induction generator are constructed by Hyundai Motor Company.

• 전력전자학회논문지
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• 제27권5호
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• pp.425-430
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• 2022

A pumped storage power station is an important means to solve the problem of peak load regulation and ensures the safety of power grid operation. The doubly fed variable-speed pumped storage (DFVSPS) system adopts a doubly fed induction machine (DFIM) to replace the synchronous machine used in traditional pumped storage. The stator of DFIM is connected to the power grid, and the three-phase excitation windings are symmetrically distributed on the rotor. Excitation current is supplied by the converter. The active and reactive power of the unit can be quickly adjusted by adjusting the amplitude, frequency, and phase of the rotor-side voltage or current through the converter. Compared with a conventional pumped storage hydropower station (C-PSH), DFVSPS power stations have various operating modes and frequent start-up and shutdown. This study introduces the structure and principle of the DFVSPS unit. Mathematical models of the unit, including a model of DFIM, a model of the pump-turbine, and a model of the converter and its control, are established. Fast power control strategies are proposed for the unit model. A 300 MW model of the DFVSPS unit is established in MATLAB/Simulink, and the response characteristics in generating mode are examined.

• Asian-Australasian Journal of Animal Sciences
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• 제14권2호
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• pp.163-169
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• 2001

Despite the high potency of the retrovirus vector system in gene transfer, one of the main drawbacks of has been difficulty in preparing highly concentrated virus stock. Numerous efforts to boost the virus titer have ended in unsatisfactory results mainly due to fragile property of retrovirus envelope protein. In this study, to overcome this problem, we constructed our own retrovirus vector system producing vector viruses encapsulated with VSV-G (vesicular stomatitis virus G glycoprotein). Concentration process of the virus stock by ultracentrifuge did not sacrifice the virus infectivity, resulting in more than 108 to 109 CFU (colony forming unit) per ml on most of the target cell lines tested. Application of this high-titer retrovirus vector system was tested on chicken embryos. Injection of virus stock beneath the blastoderms of pre-incubated fertilized eggs resulted in chick embryos expressing E. coli LacZ gene with 100% efficiency. Therefore, our results suggest that it is possible to transfer the foreign gene into chicken embryo using our high-titer retrovirus vector.

• 방송공학회논문지
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• 제16권2호
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• pp.293-304
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• 2011

본 논문에서는 H.264/AVC 인코더에서 가장 많은 연산 시간이 소요되는 움직임 추정(motion estimation, ME) 동작을 위한 하드웨어의 구조를 제안하고 IP(intellectual property) 형태로 구현하였다. 고속 움직임 추정기의 구조는 버퍼(buffer), PU 어레이(processing unit array), SAD 선택기(SAD selector), MV 생성기(motion vector generator) 등으로 구성되어 있다. PU 어레이는 16개의 PU로 구성되어 있고, 각각의 PU는 16개의 PE(processing element)로 이루어져 있다. 제안한 하드웨어의 동작적인 특징은 외부메모리 접근량을 줄이기 위해 현재와 참조프레임의 데이터를 재사용한다는 것과 SAD연산을 수행할 때 클록의 손실 없이 계산을 할 수 있다는 것이다. 구현한 고속 움직임 추정기는 Altera 사의 FPGA인 StatixIII EP3SE80F1152C2에서 3%의 자원을 사용하였고, 최대 동작주파수는 446.43MHz이었다. 따라서 구현한 하드웨어는 1080p 영상을 최대 50fps로 처리할 수 있다.

• 생명과학회지
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• 제14권2호
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• pp.315-319
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• 2004

사람의 serine palmitoyltransferase(SPT, EC 2.3.1.50)에 대한 항체를 제작하기 위하여 E. coli발현 vector인 pRset vector에 SPTLC1 및 SPTLC2 유전자를 subcloning하고 BL21 (DE3)pLys cell에 발현시켰다. 포유동물의 SPT는 원핵세포의 SPT homodimer와는 달리 SPTLC1 및 SPTLC2 2개의 sub-unit로 된 heterodimer이다. Human embryo kidney cell인 HEK293 cell의 total RNA로부터 RT-PCR을 행하여 cDNA library를 얻은 다음 SPTLC1 및 SPTLC2의 특이적인 primer 들을 이용하여 PCR을 행하였다. SPTLC1 및 SPTLC2 DNA를 hexahistidine fusion 단백질을 발현시킬 수 있는 pRset vector에 cloning하여 pRsetB/SPTLC1 및 pRsetA/SPTLC2를 얻고 염기서열을 확인하였다. 재조합 plasmid를 발현세포인 BL21 cell에 형질전환시킨 다음 ampicillin 및 chroramphenicol 배지에서 선별하여 재조합세포를 얻었다. 1 mM IPTG로서 발현을 유도하였으며 세포 단백질을 SDS-PAGE로 분리한 다음 His-tag antibody로 western blotting을 행하여 SPTLC 및 SPTLC2가 발현되었음을 확인하였다.

• 전자공학회논문지B
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• 제29B권11호
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• pp.102-110
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• 1992

In this paper, a new method of character recognition is proposed. It uses density information, in addition to positional and directional information generally used, to recognize a character. Four directional feature primitives are extracted from the thinning templates on the observation that the output of the templates have directional property in general. A simple and fast feature extraction scheme is possible. Features are organized from recursive nonary tree(N-tree) that corresponds to normalized character area. Each node of the N-tree has four directional features that are sum of the features of it's nine sub-nodes. Every feature primitive from the templates are added to the corresponding leaf and then summed to the upper nodes successively. Recognition can be accomplished by using appropriate feature level of N-tree. Also, effectiveness of each node's feature vector was tested by experiment. A method to implement the proposed feature vector organization algorithm into hardware is proposed as well. The third generation node, which is 4$\times$4, is used as a unit processing element to extract features, and it was implemented in hardware. As a result, we could observe that it is possible to extract feature vector for real-time processing.