• The Transactions of the Korean Institute of Electrical Engineers P
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• v.59 no.4
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• pp.345-350
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• 2010

This paper presents a Photovoltaic Generation system using a Space Vector Modulation. PWM voltage source inverter using inverter consists of complex type of electric power converter to compensate for the defect, that is solar cell cannot be developed continuously by connecting with the source of electric power for ordinary use. It can cause the effect of saving electric power, from 10 to 20[%]. Synchronous signal and control signal was processed by the 56F8323 microprocessor for stable modulation. Also, Waveforms output current and voltage of system controlled so that phase conforms and can supply electric power that stabilize by the unit power factor.

• Journal of the Korean Mathematical Society
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• v.58 no.1
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• pp.109-122
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• 2021

In this paper, we consider the problem of finding the hypersurface Mn in the Euclidean (n + 1)-space ℝn+1 that satisfies an equation of mean curvature type, called singular minimal hypersurface equation. Such an equation physically characterizes the surfaces in the upper half-space ℝ+3 (u) with lowest gravity center, for a fixed unit vector u ∈ ℝ3. We first state that a singular minimal cylinder Mn in ℝn+1 is either a hyperplane or a α-catenary cylinder. It is also shown that this result remains true when Mn is a translation hypersurface and u is a horizantal vector. As a further application, we prove that a singular minimal translation graph in ℝ3 of the form z = f(x) + g(y + cx), c ∈ ℝ - {0}, with respect to a certain horizantal vector u is either a plane or a α-catenary cylinder.

• Journal of Applied Biological Chemistry
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• v.53 no.1
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• pp.1-7
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• 2010

xylA promoter is a major promoter in xylose operon of Escherichia coli. xylA promoter is sufficient as the promoter for the construction of new expression vector because this promoter was tightly controlled and induced by the addition of xylose. For the construction of xylose-inducible expression vector, 600 bp of xylA promoter was ligated between AatII and HindIII of pUC18, named pXA600. In order to investigate the effect of XylR protein encoded by xylR gene on the xylA promoter, 1,988 bp of xylR gene including its promoter was ligated into downstream of multiple cloning site to the opposite direction of xylA promoter in pXA600, named pXAR600. For the measurement of expression level, 3,048 bp of lacZ structural gene was fused into xylA promoter in both plasmids pXA600 and pXAR600 as a reporter gene, named pXA600-lacZ and pXAR600-lacZ, respectively. The $\beta$-galactosidase activity of pXA600-lacZ and pXAR600-lacZ in E. coli JM109 was determined to be 1,641 and 2,304 unit by the induction with xylose in LB medium, respectively. The $\beta$-galactosidase activity of pXAR600-lacZ/JM109 was about 1.4 times higher by the induction with xylose than that of pXA600-lacZ/JM109. The $\beta$-galactosidase activity of pXA600-lacZ and pXAR600-lacZ in E.coli JM109 showed 6,282 and 9,320 unit by the induction with xylose in DM minimal medium, respectively. A regulator, xylR protein works as an activator for the gene expression by the addition of xylose in the xylose-inducible vectors because the level of gene expression in pXA600 is increased by the insertion of xylR gene into the same vector. The xynA gene of Streptomyces thermocyaneoviolaceus cloned in pXA600 and pXAR600 was successfully expressed in E. coli BLR(DE3). As a result, plasmids pXA600 and pXAR600 using xylA promoter are sufficient as new expression system to produce a foreign protein in E. coli.

• Smart Media Journal
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• v.8 no.2
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• pp.74-79
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• 2019

Traditional compound noun decomposition algorithms often face challenges of decomposing compound nouns into separated nouns when unregistered unit noun is included. It is very difficult for those traditional approach to handle such issues because it is impossible to register all existing unit nouns into the dictionary such as proper nouns, coined words, and foreign words in advance. In this paper, in order to solve this problem, compound noun decomposition problem is defined as tag sequence labeling problem and compound noun decomposition method to use syllable unit embedding and deep learning technique is proposed. To recognize unregistered unit nouns without constructing unit noun dictionary, compound nouns are decomposed into unit nouns by using LSTM and linear-chain CRF expressing each syllable that constitutes a compound noun in the continuous vector space.

• Journal of the Institute of Electronics and Information Engineers
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• v.53 no.11
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• pp.41-47
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• 2016

High efficiency video coding (HEVC) employs a coding tree unit (CTU) to improve the coding efficiency. A CTU consists of coding units (CU), prediction units (PU), and transform units (TU). All possible block partitions should be performed on each depth level to obtain the best combination of CUs, PUs, and TUs. To reduce the complexity of block partitioning process, this paper proposes the PU mode skip algorithm with region of interest (RoI) selection using motion vector. In addition, this paper presents the CU depth level skip algorithm using the co-located block information in the previously encoded frames. First, the RoI selection algorithm distinguishes between dynamic CTUs and static CTUs and then, asymmetric motion partitioning (AMP) blocks are skipped in the static CTUs. Second, the depth level skip algorithm predicts the most probable target depth level from average depth in one CTU. The experimental results show that the proposed fast CU decision algorithm can reduce the total encoding time up to 44.8% compared to the HEVC test model (HM) 14.0 reference software encoder. Moreover, the proposed algorithm shows only 2.5% Bjontegaard delta bit rate (BDBR) loss.

• Journal of Microbiology and Biotechnology
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• v.29 no.10
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• pp.1644-1655
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• 2019

Saccharomyces cerevisiae (S. cerevisiae) and glucagon-like peptide-2 (GLP-2) have been employed to improve the intestinal development of weaned animals. The goal of this study was to determine whether either exogenous S. cerevisiae or GLP-2 elicits major effects on fecal microbiotas and cytokine responses in weaned piglets. Ninety-six piglets weaned at 26 days were assigned to one of four groups: 1) Basal diet (Control), 2) empty vector-harboring S. cerevisiae (EV-SC), 3) GLP-2-expressing S. cerevisiae (GLP2-SC), and 4) recombinant human GLP-2 (rh-GLP2). At the start of the post-weaning period (day 0), and at day 28, fecal samples were collected to assess the bacterial communities via sequencing the V1-V2 region of the 16S-rRNA gene, and piglets' blood was also sampled to measure cytokine responses (i.e., IL-$1{\beta}$, TNF-${\alpha}$, and IFN-${\gamma}$). This study revealed that, on the one hand, although S. cerevisiae supplementation did not significantly alter the growth of weaned piglets, it induced increases in the relative abundances of two core genera (Ruminococcaceae_norank and Erysipelotrichaceae_norank) and decreases in the relative abundances of two other core genera (Lachnospiraceae_norank and Clostridiale_norank) and cytokine levels (IL-$1{\beta}$ and TNF-${\alpha}$) (p < 0.05, Control vs EV-SC; p < 0.05, rh-GLP2 vs GLP2-SC). On the other hand, GLP-2 supplementation had no significant influence on fecal bacterial communities and cytokine levels, but it produced better body weight and average daily gain (p < 0.05, Control vs EV-SC; p < 0.05, rh-GLP2 vs GLP2-SC). Therefore, altered fecal microbiotas and cytokine response effects in weaned piglets were due to S. cerevisiae rather than GLP-2.

• YAKHAK HOEJI
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• v.49 no.3
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• pp.217-224
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• 2005

Human Immunodeficiency Virus type 1 (HIV-l) based lentivirus vector has demonstrated great potential as gene therapy vectors mediating efficient gene delivery and long-term transgene expression in both dividing and nondividing cells. However, for clinical studies it must be confirmed that vector preparations are safe and not contaminated by replication competent lentivirus (RCL) related to the parental pathogenic virus, HIV-l. In this study, we would like to establish the method for titration and RCL detection of lentivirus vector. The titration was determined by vector expression containing the green fluorescent protein, GFP in transduced cells. The titer was $1{\times}10^7$ Transducing Unit/ml in the GFP expression assay and $8.9{\times}10^7$ molecules/ml in the real-time PCR. Also, for the detection of RCL, we have used a combination method of PCR and p24 antigen detection. First, PBS/psi and VSV-G region in the genomic DNA of transduced cells was detected by PCR assay. Second, transfer and expression of the HIV-1 gag gene was detected by p24 ELISA. In an attempt to amplify any RCL, the transduced cells were cultured for 3 weeks (amplification phase) and the supernatant of amplified transduced cell was used for the second transduction to determine whether a true RCL was present (indicator phase). Analysis of cells and supernatant at day 6 in indicator phase were negative for PBS/psi, VSV-G, and p24 antigen. These results suggest that they are not mobilized and therefore there are no RCL in amplification phase. Thus, real-time PCR is a reliable and sensitive method for titration and RCL detection of lentivirus vector.

• Journal of Power Electronics
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• v.15 no.3
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• pp.670-684
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• 2015

The hybrid cascaded multilevel inverter (HCMLI) is a popular converter topology that is being increasingly used in high power medium voltage drives. The intricacy of the control technique for a HCMLI increases with the number of levels and due to fluctuating dc voltages. This paper presents a novel offline quadrant search based space vector modulation technique to synthesize a sinusoidal output from a dispersed pattern of voltage vectors due to different voltages in the auxiliary unit. Such an investigation has never been reported in the literature and it is being attempted for the first time. The method suggested distributes the voltage vectors for a reduced total harmonic distortion at minimal computation. In addition, the proposed algorithm determines the maximum modulation index in the linear modulation range in order to synthesize a sinusoidal output for both normal and abnormal vector patterns. It is better suited for a wide range of practical applications. It is particularly well suited for renewable source fed inverters which utilize large capacitor banks to maintain the dc link, which are prone to such slow fluctuations. The proposed quadrant search space vector modulation technique is simulated using MATLAB/SIMULINK and implemented using a Nexys-2 Spartan-3E FPGA for a developed prototype.

• Biomolecules & Therapeutics
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• v.13 no.3
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• pp.143-149
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• 2005

We have demonstrated previously on a replication incompetent recombinant adenoviral vector, AdLPCD, in which the expression of cytosine deaminase (CD) gene is driven by the tumor-specific L-plastin promoter. The object of this study was to evaluate the efficacy of AdLPCD together with 5-fluorocytosine (5-FC) in suppression of the growth of established human tumor cells of ovary, Consistent with the knowledge that infection of OVCAR-3 cells with AdLPCD resulted in expression of a functional intracellular CD enzyme capable of converting 5-FC to 5-fluorouracil (5-FU) (Chung and Deisseroth, 2004), statistically significant differences in cytotoxicity were observed when AdLPCD infected cells were also exposed to 5-FC for 6 days (p=0.05), 9 days (p<0.0005) and 12 days (p<0.005), compared to 5-FC exposure alone, These results indicate that the CD gene delivered by adenoviral vector could efficiently sensitize OVCAR-3, otherwise non-toxic 5-FC. On the other hand, SKOV-3 cells, an ovarian carcinoma cell line, were more resistant to the CD/5-FC strategy compared with OVCAR-3 cells under the same condition. The results of present study suggest that the replacement of 5-FU with CD/5-FC in combination chemotherapy would be less toxic and much greater cytotoxicity than the conventional combination chemotherapy in some patients.

• Journal of Microbiology and Biotechnology
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• v.2 no.3
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• pp.174-182
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• 1992

The hemagglutinin-esterase glycoprotein (HE) gene of bovine coronavirus, coupled with a simian virus 40 early promoter and polyadenylation signal, was inserted into a human adenovirus transfer vector. The transfer vector was used to co-transfect 293 cells along with adenovirus genomic DNA. The hemagglutinin-esterase transcription unit was rescued into the adenovirus genome by homologous in vivo DNA recombination between the vector plasmid DNA and the adenovirus genomic DNA, and a recombinant adenovirus was isolated by several rounds of plaque assays. Thus the recombinant adenovirus carries the hemagglutinin-esterase gene in the early transcription region 3 (E3) of the adenovirus genome in the parallel orientation to the E3 transcription. The recombinant adenovirus synthesized the HE polypeptide in HeLa cells as demonstrated by immunoprecipitation with anti-coronavirus rabbit antisera. The recombinant HE polypeptide could be labelled by $[^3H]$glucosamine, demonstrating that the recombinant HE was glycosylated. Cells expressing the HE polypeptide exhibited hemadsorption activity when incubated with mouse erythrocytes. The HE was transported to the plasma membrane as shown by the cell surface immunofluorescence, indicating that the recombinant HE polypeptide retained its biological activities. Potential for the use of infectious recombinant adenovirus as a live virus-vectored vaccine candidate for bovine coronavirus disease is discussed.