• Journal of Korea Water Resources Association
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• v.36 no.6
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• pp.925-936
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• 2003

The objective of this study is to suggest new drawing methods of isochrones using GIS. For this purpose the Unit Hydrograph (UH) of studied watershed for instantaneous rainfall suggested by Clark have been determined by routing the time-area curve through a single linear reservoir. To evaluate constructing methods of isochrones three methods has been examined; Channel Profile and Clark-kict method; Laurenson method; Average velocity method of S.C.S. Also, these methods have been recomposed by GIS in this study. To apply first method, spatial modeling, the vector based on the stream network and Route_System measuring a distance between points has been used. A raster based on the flow direction grid from burn DEM and the slope grid from original DEM has been applied for the second method. The third method has been applied by a raster based on the landuse grid and a velocity function expressed by slope. Results by these three methods have been evaluated with observed hydrograph, and the method using average velocity method of S.C.S shows more reasonable results comparatively.

• Korean Journal of Microbiology
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• v.43 no.1
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• pp.19-22
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• 2007

Acetohydroxyacid synthase (E.C.2.2.1.6., AHAS) is the enzyme that catalyses the first step in the synthesis of the branched-chain amino acids valine, leucine and isoleucine. The AHAS gene (TIGR access code HI2585) from Heamophilus influenzae was cloned into the bacterial expression vector pET-28a and expressed in the Escherichia coli strain BL21(DE3). The expressed enzyme was purified by $Ni^{2+}-charged$ HiTrap chelating HP column. The purified enzyme appears as a single band on SDS-PAGE with a molecular mass of about 63.9 kDa. The enzyme exhibits absolute dependence on the three cofactors FAD, $MgCl_{2}$ and thiamine diphosphate for activity. Specific activity of purified enzyme has 3.22 unit/mg and optimum activity in the pH 7.5 at $37^{\circ}C$. This enzyme activity has an effect on the buffer. When comparing the enzyme activity against the organic solvent, it followed in type and the difference it is but even from the aqueous solution where the organic solvent is included with the fact that the enzyme activity is maintained.

• Korean Journal of Microbiology
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• v.47 no.1
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• pp.14-21
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• 2011

Riboflavin synthase catalyzes the formation of one molecule of each riboflavin and 5-amino-6-ribitylamino-2,4-pyrimidinedione by the transfer of a 4-carbon moiety between two molecules of the substrates, 6,7-dimetyl-8-ribityllumazine. The most remarkable feature is the sequence similarity between the N-terminal half (1-97) and the C-terminal half domain (99-213). To investigate the structure and fluorescent characteristics of the N-terminal half of riboflavin synthase (N-RS) in Escherichia coli, more than 10 mutant genes coding for the mutated N-terminal domain of riboflavin synthase were generated by polymerase chain reaction. The genes coding for the proteins were inserted into pQE vector designed for easy purification of protein by 6X-His tagging system, expressed, and the proteins were purified. Almost all mutated N-terminal domain of riboflavin synthases bind to 6,7-dimethyl-8-ribityllumazine and riboflavin as fluorescent ligands. However, N-RS C47D and N-RS ET66,67DQ mutant proteins show colorless, indicating that fluorescent ligands were dissociated during purification. In addition, most mutated proteins show low fluorescent intensity comparing to N-RS wild type, whereas N-RS C48S posses stronger fluorescent intensity than that of wild type protein. Based on this result, N-RS C48S can be used as the tool for high throughput screening system for searching for the compound with inhibitory effect for the riboflavin synthase.

• The Korean Journal of Mycology
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• v.27 no.2 s.89
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• pp.132-137
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• 1999

This study was carried out to develop the molecular marker for the detection of Pseudomonas tolaasii, a causative agent of bacterial brown blotch disease of oyster mushroom (Pleurotus ostreatus). When several primers designed from repetitive sequences and pectin lyase genes of bacteria were used to produce DNA polymorphism from different Pseudomonas spp. isolated from edible mushrooms, PEU1 primer derived from pectin lyase gene produced polymorphic bands differentiating P. tolaasii strains from other Pseudomonas species. Two bands, 1.0kb and 0.4kb, found commonly in 6 isolates of P. tolaasii were cloned into pGEM-T vector which were designated as pPTOP1 and pPTOP2, respectively, to use as probe. The 0.4 kb insert of pPTOP2 hybridized to only 6 isolates of P. tolaasii, but did not to the other Pseudomonas species. As few as $1.5{\times}10^3$ colony forming unit (cfu) of P. tolaasii could be detected by dot blot hybridization with the cloned 0.4kb DNA in pPTOP2.

• BMB Reports
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• v.38 no.6
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• pp.683-689
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• 2005

Antibody to hepatitis B surface antigen (HBsAb) is the important serological marker of the hepatitis B virus (HBV) infection. Conventionally, the hepatitis B surface antigen (HBsAg) obtained from the plasma of HBV carriers is used as the diagnostic antigen for detection of HBsAb. This blood-origin antigen has some disadvantages involved in high cost, over-elaborate preparation, risk of infection, et al. In an attempt to explore the suitable recombinant HBsAg for the diagnostic purpose, the HBV S gene was expressed in Pichia pastoris and the product was applied for detection of HBsAb. Hepatitis B virus S gene was inserted into the yeast vector and the expressed product was analyzed by sodium dodecyl sulphate polyacrolamide gel electrophoresis (SDS-PAGE), immunoblot, electronic microscope and enzyme linked immunosorbent assay (ELISA). The preparations of synthesized S protein were applied to detect HBsAb by sandwich ELISA. The S gene encoding the 226 amino acid of HBsAg carrying ahexa-histidine tag at C terminus was successfully expressed in Pichia pastoris. The His-Tagged S protein in this strain was expressed at a level of about 14.5% of total cell protein. Immunoblot showed the recombinant HBsAg recognized by monoclonal HBsAb and there was no cross reaction between all proteins from the host and normal sera. HBsAb detection indicated that the sensitivity reached 10 mIu (micro international unit)/ml and the specificity was 100% with HBsAb standard of National Center for Clinical Laboratories. A total of 293 random sera were assayed using recombinant S protein and a commercial HBsAb ELISA kit (produced by blood-origin HBsAg), 35 HBsAb positive sera and 258 HBsAb negative sera were examined. The same results were obtained with two different reagents and there was no significant difference in the value of S/CO between the two reagents. The recombinant HBV S protein with good immunoreactivity and specificity was successfully expressed in Pichia pastoris. The reagent for HBsAb detection prepared by Pichia pastoris-derived S protein showed high sensitivity and specificity for detection of HBsAb standard. And a good correlation was obtained between the reagent produced by recombinant S protein and commercial kit produced by blood-origin HBsAg in random samples.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.2
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• pp.440-447
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• 2020

The purpose of this study is to identify the causation and variation among the various types of onion prices in the major production sites to predict these prices. The Granger causal relationship was tested on the basis of VECM by setting the onion price of the early, middle, and late species as individual variables. The analysis shows that the amount of onions produced in the prior term affects the price of onions for the later period, while garlic in the substitution relationship with onions also affects the prices of onions for the early and middle-variety. On the other hand, the price of the late-variety is affected by the price of the early-variety, and the price of the middle-variety is also affected by the price of the early-variety. If the price of onions on Jeju changes due to other factors, the prices of onions in Jeollanam-do and Gyeongsangnam-do provinces will be affected. Accordingly, when the production of late-variety increases or decreases in production under any factor and to promote stability of the prices of middle and late-variety through preemptive supply and demand measures when the prices of ultra-breed onions rise or fall due to any factor (Ed- I cannot understand this last sentence and cannot guess at the correct meaning. Please try to rewrite very simply).

• Journal of Energy Engineering
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• v.29 no.1
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• pp.1-12
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• 2020

The rapid climate change is strengthening carbon emissions regulations internationally. Korea is strongly pressed to accept the obligation to reduce greenhouse gases as one of the United Nations Framework Convention on Climate Change. This article analyzed the Granger causalities among environmental regulation, economic growth, electricity consumption, and CO₂ emission in Korea, using unit root test, cointegration test, and vector error correction model. As the results, environmental regulation has shown the bidirectional causalities with electricity consumption and CO₂ emission, while being unilaterally affected by economic growth in the long-run and strong relationship. Economic growth has affected electricity consumption, CO2 emission, and environmental regulation in the long-run, in the complex structure of the unilateral and short-run causality with electricity consumption and the bidirectional causality with CO2 emission. The policy implications will be as follows: ① environmental regulation should induce sustainable growth through encouraging technological innovation relating to CO2 reduction and productivity enhancement. ② Responding to the international CO2 reduction regulation, the synthetic policy initiatives will be considered to make synergy effects among policies relating to economic growth, electricity consumption.

• Horticultural Science & Technology
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• v.29 no.2
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• pp.116-122
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• 2011

In this study, we report the generation and analysis of 1,392 expressed sequence tags (ESTs) from Korean Stewartia (Stewartia koreana Nakai). A cDNA library was generated from the young leaf tissue and a total of 1,392 cDNA were partially sequenced. EST and unigene sequence quality were determined by computational filtering, manual review, and BLAST analyses. Finally, 1,301 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length 100 nucleotides. A total of 893 unigene, consisting of 150 contigs and 743 singletons, was identified after assembling. Also, we identified 95 new microsatellite-containing sequences from the unigenes and classified the structure according to their repeat unit. According to homology search with BLASTX against the NCBI database, 65% of ESTs were homologous with known function and 11.6% of ESTs were matched with putative or unknown function. The remaining 23.2% of ESTs showed no significant similarity to any protein sequences found in the public database. Annotation based searches against multiple databases including wine grape and populus sequences helped to identify putative functions of ESTs and unigenes. Gene ontology (GO) classification showed that the most abundant GO terms were transport, nucleotide binding, plastid, in terms biological process, molecular function and cellular component, respectively. The sequence data will be used to characterize potential roles of new genes in Stewartia and provided for the useful tools as a genetic resource.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.6
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• pp.187-193
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• 2015

The ICT healing platform sets a couple of goals including preventing chronic diseases and sending out early disease warnings based on personal information such as bio-signals and life habits. The 2-step open system(TOS) had a relay designed between the healing platform and the storage of personal health data. It also took into account a publish/subscribe(pub/sub) service based on large-scale connections to transmit(monitor) the data processing process in real time. In the early design of TOS pub/sub, however, the same buffers were allocated regardless of connection idling and type of message in order to encode connection messages into a deflate algorithm. Proposed in this study, the dynamic buffer allocation was performed as follows: the message transmission type of each connection was first put to queuing; each queue was extracted for its feature, computed, and converted into vector through tf-idf, then being entered into a k-means cluster and forming a cluster; connections categorized under a certain cluster would re-allocate the resources according to the resource table of the cluster; the centroid of each cluster would select a queuing pattern to represent the cluster in advance and present it as a resource reference table(encoding efficiency by the buffer sizes); and the proposed design would perform trade-off between the calculation resources and the network bandwidth for cluster and feature calculations to efficiently allocate the encoding buffer resources of TOS to the network connections, thus contributing to the increased tps(number of real-time data processing and monitoring connections per unit hour) of TOS.

• Journal of Korean Society of Forest Science
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• v.88 no.1
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• pp.1-10
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• 1999

The dynamic relationships among import prices of roundwood are analyzed using the time series approach. A vector autoregression(VAR) model is estimated for six import prices(New Zealand, Chile, Russia, U.S.A., PNG, and Malaysia). Then Granger's causality test, variance decomposition analysis, and impulse response function analysis are also conducted. The major results are summarized as follows : (1) The prices of New Zealand and Russia are caused by only own lagged prices. (2) The prices of Chile and PNG are effected by New Zealand, the price of PNG is effected by New Zealand and Russia, and the price of U.S.A. is effected by those of Chile and PNG, respectively. (3) An exogenous shock in New Zealand will affect the prices of New Zealand, PNG, U.S.A., Chile, Russia. (4) An exogenous shock in Chile may also affect the prices of Chile, U.S.A., Russia.