• Journal of Astronomy and Space Sciences
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• 제32권1호
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• pp.81-89
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• 2015

In this paper, we describe the development of a bioreactor for a cell-culture experiment on the International Space Station (ISS). The bioreactor is an experimental device for culturing mouse muscle cells in a microgravity environment. The purpose of the experiment was to assess the impact of microgravity on the muscles to address the possibility of long-term human residence in space. After investigation of previously developed bioreactors, and analysis of the requirements for microgravity cell culture experiments, a bioreactor design is herein proposed that is able to automatically culture 32 samples simultaneously. This reactor design is capable of automatic control of temperature, humidity, and culture-medium injection rate; and satisfies the interface requirements of the ISS. Since bioreactors are vulnerable to cell contamination, the medium-circulation modules were designed to be a completely replaceable, in order to reuse the bioreactor after each experiment. The bioreactor control system is designed to circulate culture media to 32 culture chambers at a maximum speed of 1 ml/min, to maintain the temperature of the reactor at $36{\pm}1^{\circ}C$, and to keep the relative humidity of the reactor above 70%. Because bubbles in the culture media negatively affect cell culture, a de-bubbler unit was provided to eliminate such bubbles. A working model of the reactor was built according to the new design, to verify its performance, and was used to perform a cell culture experiment that confirmed the feasibility of this device.

• 한국환경성돌연변이발암원학회지
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• 제23권2호
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• pp.56-62
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• 2003

The liver progenitor cells could form a potential target cell population fore both tumor-initiating and -promoting chemicals. Induction of drug-metabolizing and antioxidant enzymes, including AhR-dependent CYP1A1, NQO-1 and AKR1C9, was detected in the rat liver epithelial WB-F344 "stem-like" cells. Additionally, WB-F344 cells express a functional, wild-type form of p53 protein, a biomarker of genotoxic events, and connexin 43, a basic structural unit of gap junctions forming an important type of intercellular communication. In this cellular model, two complementary assays have been established for detection of the modes of action associated with tumor promotion: inhibition of gap junctional intercellular communication (GJIC) and proliferative activity in confluent cells. We found that the PAHs and PCBs, which are AhR agonists, released WB-F344 cells from contact inhibition, increasing both DNA synthesis and cell numbers. Genotoxic effects of some PAHs that lead to apoptosis and cell cycle delay might interfere with the proliferative activity of PAHs. Contrary to that, the nongenotoxic low-molecular-weight PAHs and non-dioxin-like PCB congeners, abundant in the environment, did not significantly affect cell cycle and cell proliferation; however both groups of compounds inhibited GJIC in WB-F344 cells. The release from contact inhibiton by a mechanism that possibly involves the AhR activation, inhibition of GJIC and genotoxic events induced by environmental contaminants are three important modes of action that could play an important role in carcinogenic effects of toxic compounds. The relative potencies to inhibit GJIC, to induce AhR-mediated activity, and to release cells from contact inhibition were determined for a large series of PAHs and PCBs and their metabolites. In vitro bioassays based on detection of events on cellular level (deregulation of GJIC and/or proliferation) or determination of receptor-mediated activities in both ?$stem-like^{\circ}{\times}$ and hepatocyte-like liver cellular models are valuable tools for detection of modes of action of polyaromatic hydrocarbons. They may serve, together with concentration data, as a first step in their risk assessment.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.6039-6046
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• 2015

Aims: To investigate effect of metallic nanoparticles, silver (AgNPs) and gold nanoparticles (AuNPs) as antitumor treatment in vitro against human breast cancer cells (MCF-7) and their associated mechanisms. This could provide new class of engineered nanoparticles with desired physicochemical properties and may present newer approaches for therapeutic modalities to breast cancer in women. Materials and Methods: A human breast cancer cell line (MCF-7) was used as a model of cells. Metallic nanoparticles were characterized using UV-visible spectra and transmission electron microscopy (TEM). Cytotoxic effects of metallic nanoparticles on MCF-7 cells were followed by colorimetric SRB cell viability assays, microscopy, and cellular uptake. Nature of cell death was further investigated by DNA analysis and flow cytometry. Results: Treatment of MCF-7 with different concentrations of 5-10nm diameter of AgNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $6.28{\mu}M$, whereas treatment of MCF-7 with different concentrations of 13-15nm diameter of AuNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $14.48{\mu}M$. Treatment of cells with a IC50 concentration of AgNPs generated progressive accumulation of cells in the S phase of the cell cycle and prevented entry into the M phase. The treatment of cells with IC50 concentrations of AuNPs similarly generated progressive accumulation of cells in sub-G1 and S phase, and inhibited the entrance of cells into the M phase of the cell cycle. DNA fragmentation, as demonstrated by electrophoresis, indicated induction of apoptosis. Conclusions: Our engineered silver nanoparticles effectively inhibit the proliferation of human breast carcinoma cell line MCF-7 in vitro at high concentration ($1000{\mu}M$) through apoptotic mechanisms, and may be a beneficial agent against human carcinoma but further detailed study is still needed.

• 설비공학논문집
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• 제30권2호
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• pp.92-99
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• 2018

A numerical analysis of a compressor dehumidifier has been conducted focusing on the air side heat transfer, which is a part of a series research on the dehumidifier. The moving reference frame was applied to the fan modeling, and the porous model was used for the evaporator and condenser modeling. Curve fitting obtained the inertial and viscous resistances parameters to the results of the physical model of the unit cell with actual shape of a fin tube. The porous model was validated within a reasonable computation time for the range of practical inlet velocity of a dehumidifier. A parametric study has been conducted for fin number, fan speed (i.e., air flow rate), and evaporator/condenser tube arrangement. ANOVA analysis showed the dependency of each parameter on the velocity and temperature uniformity, which are desirable for high performance of the dehumidifier.

• 농촌지도와개발
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• 제27권2호
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• pp.75-87
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• 2020

The purpose of this study is to investigate the location characteristics of the rice paddy fields and to predict shrinking areas of them. The study area is in South Korea nationwide, and a 300×300m raster level is adopted as a spatial analysis unit. The binary logit model and spatial simulation model are employed for the analyses. As a result, population, industry, climate and nature, and accessibility play a significant role in determining rice paddy fields' locations. It is predicted that the shrinking rate will be high in Gangwon-do, Gyeongsangbuk-do, and Gyeongsangnam-do until 2027. The hot spots are intensively shaped in the inland of Gangwon-do, eastern parts of Gyeonggi-do, and the borders of Gyeongsangnam-do and Jeollabuk-do between Charyeong and Sobaek mountain ranges. Using Gimcheon-si as an example, the study suggests fundamental policy implications for taking advantage of the simulation results from the lens of local agriculture. Several policy measures are proposed for improving management strategies for the rice paddy fields in the long run.

• 대한화학회지
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• 제26권6호
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• pp.414-420
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• 1982

유한요소법을 이용하여 니켈입자가 분산된 폴리에틸렌의 기계적 성질을 추정하였다. 이 해석에서 다음 2단계의 과정을 통해 조사하였다. 첫 단계는 모재의 중심부에 구상의 물질을 포함하는 이른바 단일요소 모델을 생각하여 이것을 유한요소법에 의해 계산하였고, 다음 단계는 이러한 단일요소 모델이 모여 전체를 구성하고 있으므로 이 단일 모델이 조합된 형태의 전체 모델을 설정하여 해석 하는 것이다. 이 두 과정에서 니켈과 폴리에틸렌의 두상만을 고려한 2상 모델과, 니켈과 폴리에틸렌의 복합재료에서 이른 바 transcrystalline 구역이 관찰되어 이 구역을 고려한 3상 모델의 두 모델을 각각 사용하여 그 차이를 살펴 보았다. 유한요소법은 임의의 하중에서 응력 및 변위의 계산을 가능하게 한다. 이 컴퓨터로 계산된 출력으로 부터 탄성계수, poisson 비 및 복합재료의 응력 분포등을 얻을 수 있었다. 이 유한요소법에 의한 계산과 인장 실험을 통한 실험결과는 서로 잘 일치한다.

• 복합신소재구조학회 논문집
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• 제2권3호
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• pp.1-11
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• 2011

본 연구에서는 하위 요소로(sub-element) 구성된 3차원 대칭 단위 요소들로 조합된 트러스 격자 구조물의 연속적인 물성치를 제안하였다. 개별적인 트러스 격자 물성치는 균질화 작업을 통하여 유효한 응력과 변형률 관계로 이루어진 연속적인 물성치 모델로 나타낼 수 있다. 미시적인 규모(micro scale) 스트럿의 인장이나 압축 응답에 의한 축강성은 전체 격자재료의 대부분의 강도를 차지하고, 이러한 스트럿의 부피 분율(fraction)은 효과적인 강도뿐만 아니라 복제 가능한 단위 요소로 이루어진 격자판의 상대밀도에 큰 영향을 주었다. 그러므로 균질한 강성부재로 구성된 연속적인 구성모델은 미시적인 규모로 간주되는 스트럿의 강도, 내부응력 상태 및 부피 분율과 관련된 역학적인 특성들을 포함하고 있다는 것을 확인할 수 있었다. 미시적인 규모의 응력에서 소성흐름은 균질한 구성식에서 파생된 거시적인 규모에서의 (macro-scale)응력 표면에 있는 연속적인 응력함수의 영역을 확장한다. 따라서 본 연구를 통하여 3차원 대칭 단위요소 구조물의 기본 기하학을 조사하고 압력에 의존적인 마크로 규모에서의 (macro-scale) 응력함수를 예측하는 연속적인 소성모델을 공식화하였다.

• 한국항공우주학회지
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• 제34권10호
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• pp.24-31
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• 2006

본 논문에서는 유연수지 복합재료에 대하여 기하학적 비선형해석을 수행하였다. 실제 랜덤한 섬유배열을 사각배열과 육각배열로 가정하고 각각에 대해 단위구조를 정의하였다. 다양한 하중상태를 수치적으로 모사하여 단위구조해석을 통해 전체 구조물의 응력-변형률 선도를 예측하였고 이로부터 등가물성치를 계산하였다. 해석시 유연수지의 초탄성 성질을 정의하기 위해 Mooney-Rivlin모델을 사용하였다. 계산결과, 유연수지 복합재료 구조물은 변형률 증가에 따라 비선형의 응력-변형률 관계를 보였다. 비선형성은 횡방향 하중 상태에서 더욱 두드러지게 나타났으며, 이 경우 복합재 단면의 섬유배열 형태에 따라 상당한 차이를 보여주었다.

• 한국산학기술학회논문지
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• 제13권12호
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• pp.6022-6026
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• 2012

본 논문은 마이크로스트립 전송선로에 삽입된 주파수 선택 표면(FSSs)을 사용하여 주기적인 구조를 갖는 대역저지필터(BSF)에 관한 것이다. 제안된 대역저지필터는 변형된 크로스 루프 슬롯 (Cross-loop slot)을 단위 셀로 하여 주기적인 구조로 설계하였으며, 설계된 대역 저지 필터는 등가회로 모델과 분산 도표(dispersion diagram)로 해석하였다. 대역 저지 필터의 중심주파수(fo)는 6.6 GHz이다. 제안된 필터는 단위 셀의 개수가 증가하면 3dB 대역폭은 넓어지고 삽입손실은 줄어드는 특성이 있다. 또한 제안한 대역저지필터는 소형의 평면구조를 가지며, 한 번의 에칭으로 제작이 가능 하여 다양한 분야의 RF 필터로서 사용 가능성이 높다.

• Steel and Composite Structures
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• 제49권4호
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• pp.393-405
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• 2023

In this paper, the energy absorption capability of a novel cruciform composite lattice structure was evaluated through the simulation of compression tests. For this purpose, several test samples of Polylactic acid cellular reinforced with continuous glass fibers were prepared for compression testing using the additive manufacturing method of material extrusion. Using a conventional path design for material extrusion, multiple debonding is probable to be occurred at the joint regions of adjacent cells. Therefore, an innovative printing path design was proposed for the cruciform lattice structure. Afterwards, quasistatic compression tests were performed to evaluate the energy absorption behaviour of this structure. A finite element model based on local material property degradation was then developed to verify the experimental test and extend the virtual test method. Accordingly, different combinations of unit cells' dimensions using the design of the experiment were numerically proposed to obtain the optimal configuration in terms of the total absorbed energy. Having brilliant energy absorption properties, the studied cruciform lattice with its optimized unit cell dimensions can be used as an energy absorber in crashworthiness applications. Finally, a cellular structure will be suitable with optimal behavior in crush load efficiency and high energy absorption.