• 한국토양동물학회지
• /
• 제6권1_2호
• /
• pp.37-44
• /
• 2001

제주시의 한라수목원과 서귀포시의 한라수목원 직영 육묘장에서 1999년과 2001년 수목의 뿌리를 가해하는 굼벵이의 종류를 조사하였다. 1999년 3월에는 한라수목원에서 12과 14종의 수종을 대상으로 조사를 하였고, 서귀포 육묘장에서는 주목 묘목에서 굼벵이를 조사하였다. 2001년에는 3월과 9월 한라수목원에서 27과 55종의 수목을 대상으로 조사를 하였으며, 서귀포 육묘장에서는 9과 18종의 수목에서 조사를 하였다. 한라수목원에서는 6종의 굼벵이와 풍뎅이가 채집이 되었는데 큰다색풍뎅이 (Holotrichia niponensis)는 21과 28종의 수목에서 채집되었고, 참검정풍뎅이 (H.diomphalia)는 20과 27종에서 채집이 되었으며, 미동정 굼벵이가 19과 27종의 수목에서 채집되었다. 애우단풍뎅이(Maladera orientalis)는 4과 4종의 수목에서 채집되었고, 똥풍뎅이 1종(Aphodius sp.)과 주둥무늬차색풍뎅이는 석류(Punica granatum)와 참개암나무(Carpinus sieboldiana)에서만 성충으로 채집되었다. 서귀포시의 육묘장에서는 큰다색풍뎅이가 6과 9종의 묘목에서 채집되었고, 참검정풍뎅이는 6과 10종의 묘목에서 채집되었다. 후피향나무(Ternss-traermia japonica)에서는 별줄풍뎅이(Minela testaceopes) 유충이 채집되었다. 큰다색풍뎅이와 참검정풍뎅이, 미동정 굼벵이 1종이 우점종이었으며 녹나무(Cinumomum cam-phrora)에서는 이들이 모두 피해를 주고 있었고, 주목에서는 큰다색풍뎅이의 피해가 심각하였다.

• Journal of Species Research
• /
• 제13권2호
• /
• pp.159-177
• /
• 2024

A faunal study on the marine amphipods at eight sites of the continental shelf region in the Southern Sea, Korea was conducted from 16-17 August 2022. In this study, 26 amphipod species in 19 genera and 14 families were found. Among these 14 families, the family Ampeliscidae with six species showed the highest abundance, followed by the family Caprellidae with five species. Amphipods were collected using conical nets, MOCNESS nets, SM grabs, and trawls. When using the SM Grab for collecting benthic sediment, 15 out of a total of 26 amphipod species were surveyed, indicating the highest diversity of amphipod species. Out of the total 26 species, four identified species (Ampelisca pygmaea Schellenberg, 1938; Byblis longiflagelis Ren, 1998; Caprella iniquilibra Mayer, 1903; Primno latreillei Stebbing, 1888) and five unidentified species(Themisto sp., Liljeborgia sp., Dulichiella sp., Cranocephalus sp., Primno sp.) are newly recorded in Korean fauna. These newly recorded species are fully illustrated and compared with related species. We provide a list amphipod fauna in Southern Sea of Korea including figures and tables.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.115.1-115
• /
• 2003

Post-harvest decay, caused by Penicillium spp. is a serious problem of fruits worldwide. Morphological characteristics and molecular markers were used to characterize 22 Penicillium isolates from apples, 18 isolates from pears, 60 from oranges and 18 from grapes and 23reference isolates representing related Penicillium spp. to assess their diversity and resolve their taxonomy. Based on morphological and physiological characteristics, the isolates were grouped as identical or very similar to P. digitatum, P. italicum, P. ulaiense or very similar to P. crustosum, P. expansum, P. solitum and unidentified Penicillium spp. Based on sequence comparisons of ITS region, variable site were presented within and among the species, but there variation were not correlated with the species. Cluster analyses of AP-PCR fragment patterns using UP and L45 primer and the -tubulin gene sequence, the Penicillium species were segregated into distinct groups. Particularly. the -tubulin partial sequence data provided support for species concepts based on morphological and physiological characteristics.

• Environmental Analysis Health and Toxicology
• /
• 제11권1_2호
• /
• pp.49-57
• /
• 1996

The production of reactive oxygen species on addition of hexavalent chromium (potassium dichromate, $K_2Cr_2O_7$ ) to lung cells in culture was studied using flow cytometer analysis. A Coulter Epics Profile flow cytometer was used to detect the formation of reactive oxygen species after $K_2Cr_2O_7$ was added to A549 cells grown to confluence. The cells were loaded with the dye, 2',7'-dichlorofluorescein diacetate, after which cellular esterases removed the acetate groups and the dye was trapped intracellularly. Reactive oxygen species oxidized the dye, with resultant fluorescence. Increased doses of Cr(VI) caused increasing fluorescence (10-fold higher than background at 200 gM). Addition of Cr(III) compounds, as the picolinate or chloride, caused no increased fluorescence. Electron paramagnetic resonance (EPR) spectroscopic studies indicated that three (as yet unidentified) spectral "signals" of the free radical type were formed on addition of 20, 50, 100 and 200 gM Cr(VI) to the A549 cells in suspension. Two other EPR 'signals" with the characteristics of Cr(V) entities were seen at field values lower than the standard free radical value. radical value.

• Journal of Species Research
• /
• 제11권3호
• /
• pp.162-168
• /
• 2022

Compared to marine dinoflagellates, freshwater species are rarely recorded in Korea. In the present study, we isolated a freshwater dinoflagellate, Palatinus, from the Paldang Reservoir, Korea, in December 2021. The overall cell shape was ovoid, and the cell size was 34.3 ㎛ in length (25.8-39.5 ㎛) and 28.4 ㎛ in width (21.5-34 ㎛). An eyespot was usually observed near the sulcal region. The Kofoidian plate formula of the species was determined to be 4', 2a, 7", 6c, 5s, 5''', and 2''''. Apical pore complex was not observed. However, variations in the cingular plate caused by the fusion of 3C and 4C were observed. Analyses of 28S rDNA sequences revealed that the unidentified species is 100% similar to Palatinus apiculatus, and clustered together in the same lineage in the phylogenetic tree (100% bootstrap value). Our findings confirmed that the isolated dinoflagellate is Palatinus apiculatus, which was discovered for the first time in Korean freshwaters.

• ALGAE
• /
• 제28권4호
• /
• pp.307-330
• /
• 2013

The genus Cryptomonas is easily recognized by having two flagella, green brownish color, and a swaying behavior. They have relatively simple morphology, and limited diagnostic characters, which present a major difficulty in differentiating between species of the genus. To understand species delineation and phylogenetic relationships among Cryptomonas species, the nuclear-encoded internal transcribed spacer 2 (ITS2), partial large subunit (LSU) and small subunit ribosomal DNA (rDNA), and chloroplast-encoded psbA and LSU rDNA sequences were determined and used for phylogenetic analyses, using Bayesian and maximum likelihood methods. In addition, nuclear-encoded ITS2 sequences were predicted to secondary structures, and were used to determine nine species and four unidentified species from 47 strains. Sequences of helix I, II, and IIIb in ITS2 secondary structure were very useful for the identification of Cryptomonas species. However, the helix IV was the most variable region across species in alignment. The phylogenetic tree showed that fourteen species were monophyletic. However, some strains of C. obovata had chloroplasts with pyrenoid while others were without pyrenoid, which used as a key character in few species. Therefore, classification systems depending solely on morphological characters are inadequate, and require the use of molecular data.

• Mycobiology
• /
• 제50권4호
• /
• pp.231-237
• /
• 2022

Penicillium species have been actively studied in various fields, and many new and unrecorded species continue to be reported in Korea. Moreover, unidentified and misidentified Korean Penicillium species still exist in GenBank. Therefore, it is necessary to revise the Korean Penicillium inventory based on accurate identification. We collected Korean Penicillium nucleotide sequence records from GenBank using the newly developed software, GenMine, and re-identified Korean Penicillium based on the maximum likelihood trees. A total of 1681 Korean Penicillium GenBank nucleotide sequence records were collected from GenBank. In these records, 1208 strains with four major genes (Internal Transcribed Spacer rDNA region, b-tubulin, Calmodulin and RNA polymerase II) were selected for Penicillium reidentification. Among 1208 strains, 927 were identified, 82 were identified as other genera, the rest remained undetermined due to low phylogenetic resolution. Identified strains consisted of 206 Penicillium species, including 156 recorded species and 50 new species candidates. However, 37 species recorded in the national list of species in Korea were not found in GenBank. Further studies on the presence or absence of these species are required through literature investigation, additional sampling, and sequencing. Our study can be the basis for updating the Korean Penicillium inventory.

• 환경생물
• /
• 제17권1호
• /
• pp.71-77
• /
• 1999

고리원전 주변 해역에서 1995년 11월부터 1996년 12월까지 총 13회의 현장조사를 수행하였다. 그 결과 식물플랑크톤은 총 162개 분류군(미동정종 포함)이 출현하였으며 그중 규조류가 총 120종이 출현하여 가장 많은 종수를 보였고 규질편모류 3종, 와편모조류 34종, 유글레나조류 2종, 녹조류 1종, 기타편모류 2종 등이 출현하였다. 규조류 종인 Skeletonema costatum은 연중 우점종으로 기록되었다. 식물플랑크톤 현존량의 월 평균값 변화는 최저 94 cells/ml(8월)에서 최고 1059 ce11s/m1(7월)에 이르며 2월과 7월에 현존량의 peak가 나타났다. 고리원전 주변해역에서는 온배수에 의한 영향이 계절에 따라 차별적으로 나타나고 겨울과 가을철에 한하여 1∼3 km의 수역에서 식물플랑크톤 현존량의 증가가 일어 날 수 있음을 나타내었고 여름철 식물플랑크톤 현존량은 온배수의 영향이 상당히 부정적임을 나타내었다.

• Journal of Microbiology
• /
• 제44권5호
• /
• pp.572-576
• /
• 2006

To obtain primary idea on oral bacterium species that are generally present in periodotally healthy Koreans, the oral bacterial flora in the saliva of four periodontally healthy Koreans at different ages (5, 32, 35, 65) was investigated in this study. For this investigation, 16S rRNA gene clone libraries were generated from the saliva of the four healthy Koreans, and 50 clones were randomly selected from each saliva clone library and sequenced. Totally, 37 different kinds of bacterial 16S rRNA gene sequences were identified based on sequence homology search through GenBank database. The 37 kinds of saliva clone sequences were classified to 14 genera and 2 uncultured and 1 unidentified bacteria. Among the 14 identified genera, Streptococcus, Prevotella, and Veillollella were common genera, and Streptococcus was dominant genus that accounted for 7 different species. Among the seven Streptococcus species, S. salivarius appeared as the most common species. More numbers of species belonging to the genera Streptococcus and Prevotella was present in saliva from ages 32 and 35. While saliva from ages 5 and 65 showed more numbers of species belonging to the genera Rothia, including potential pathogenic species. Overall, saliva of a young child and a senior showed higher bacterial diversity than that of young adults.

• Journal of Microbiology and Biotechnology
• /
• 제17권1호
• /
• pp.168-175
• /
• 2007

We report here on the cultivation of numerous novel bacterial species from a eutrophic freshwater pond. A total of 60 strains, 15 strains per each culture medium, were obtained from the surface of a eutrophic freshwater pond by employing a conventional dilution-plating method with four different kinds of culture media, including R2A, 1/10R2A, PCA, and 1/10PCA. Among the 60 strains isolated, 27 strains showed less than 97% 16S rRNA gene sequence similarities to validly published species, and thus they are considered to comprise 19 novel species. Of the 27 strains assigned to the novel species, the majority of the strains (20 strains) were affiliated with the Alphaproteobacteria and Betaproteobacteria. The remaining 7 strains were affiliated with the Gammaproteobacteria, Firmicutes, Actinobacteria, and Deinococci. Because we have isolated 19 novel species from a usual freshwater pond using a conventional culturing technique, our results suggest that an unexplored ecosystem, even if it looks like a common ecosystem found elsewhere, harbors diverse unidentified microbes, which will be definitely further characterized.