• Title/Summary/Keyword: Unidentified species

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Seasonal variation of Attached Diatoms community in the Hantan River

  • Kim, Yong-Jae
    • Proceedings of the Korea Society of Environmental Biology Conference
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    • 2004.05a
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    • pp.24-24
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    • 2004
  • This study was investigated to the seasonal variation of attached diatoms communities in the Hantan River in November 2001, February, Ma and August 2002. It was identified the total 107 taxa which were composed of 95 species, 11 varieties and 1 unidentified speCies. The standing crops ranged 298,5004,776,000cells${\cdot}cm^{-2}.$and showed marked irregularly seasonal variations. It was higher value at the lower station than the upper station in fall. But it was similar values from the upper to the lower station by the effect of typhoon in summer. Chlorophyll concentrations ranged $13.4~304.2{\mu}m{\cdot}cm^{-2}.$ Standing crops and chlorophyll showed similar trends during investigation. Low diversity index values probably indicated the effects of envi~onmental stresses(water temperature, flow and current velocity) other than organic pollution. An assessment of organic pollution using epilithic diatoms(DAlpo) was $\alpha$-oligosaprobic states at the upper and mid stations, and was $\alpha$-mesosaprobic states at the lower stations.

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Note on the Prey Items of Marine Cladocerans (해산지각류의 먹이에 관한 보고)

  • KIM, SE-WHA;YOON, YANG-HO;ONBE, TAKASHI
    • 한국해양학회지
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    • v.28 no.1
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    • pp.69-71
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    • 1993
  • Food items of the five species of marine cladocerans were reported to be largely confined to centric diatoms. Reexamination of the prey items, however, revealed that flagellar organisms were also well fed by cladocerans. This additional finding suggests that marine cladocerans might extend their food items to weakly motile flagellates e.g., Prorocentrum micans, Protoperidinium sp. and unidentified flagellates.

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Comparison of electrophoretic karyotypes in fusarium

  • Min, Byung-Re
    • Journal of Microbiology
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    • v.33 no.4
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    • pp.334-338
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    • 1995
  • The electrophoretic karyotypes of 6 species in different Fusarium sections were examined by using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Intact chromosomal DNA was prepared from protoplasts and up to 9 distinct bands were separated on 0.7% or 0.8% agarose gel under several different conditions. Putative chromosome numbers varied from 6 to 9 amd polymorphic karyotypes were observed in different Fusarium sections. Using Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as standards, the sizes of the Fusarium spp. chromosomes were estimated. The electrophoretic karyotypes of F. moniliforme and F. subglutinans (section Liseola) were similar. Unidentified filamentour fungi, F. beomiforme was much closer to F. axysporum (section Elecgans) in karyotype and the karyotypes of F. napiforme were more similar to those of section Liseola than any other sections. F. graminearum (section Discolor) had a distinctive electrophoretic karyotype.

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Ozobranchus jantseanus (Clitellata: Ozobranchidae) from Reeve's Turtle, Mauremys reevesii: New Annelid Fauna in Korea

  • Koo, Kyo Soung;Yun, Kayoung;Jang, Yikweon
    • Parasites, Hosts and Diseases
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    • v.60 no.3
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    • pp.213-215
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    • 2022
  • Turtle leeches have not been recorded in Korea, although they occur in geographically adjacent countries including China and Japan. This study describes a turtle leech, Ozobranchus jantseanus (Clitellata: Ozobranchidae), found from Reeve's turtle (Mauremys reevesii) in Korea. Of the 143 Reeve's turtles collected from the freshwater reservoir in Jinju City, Gyeongsangnam-do, Korea, 95 unidentified leeches were found in 8 (5.6%) individuals. The leeches had 22 somites with 11 pairs of externally exposed branchiae, body-sized posterior suckers, and spines on the dorsal surface. We identified these leeches as Ozobranchus jantseanus Oka, 1912 (Clitellata: Ozobranchidae). This species of turtle leeches found in Korea may fill the gap in the biodiversity of East Asian annulus.

Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia

  • Khaerani Nurlaelita;Arif Wibowo;Ani Widiastuti
    • The Plant Pathology Journal
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    • v.40 no.4
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    • pp.399-407
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    • 2024
  • Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch's Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.

Rapid metabolic discrimination between Zoysia japonica and Zoysia sinica based on multivariate analysis of FT-IR spectroscopy (FT-IR스펙트럼 데이터의 다변량통계분석 기반 들잔디와 갯잔디의 대사체 수준 신속 식별 체계)

  • Yang, Dae-Hwa;Ahn, Myung Suk;Jeong, Ok-Cheol;Song, In-Ja;Ko, Suk-Min;Jeon, Ye-In;Kang, Hong-Gyu;Sun, Hyeon-Jin;Kwon, Yong-Ik;Kim, Suk Weon;Lee, Hyo-Yeon
    • Journal of Plant Biotechnology
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    • v.43 no.2
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    • pp.213-222
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    • 2016
  • This study aims to establish a system for the rapid discrimination of Zoysia species using metabolite fingerprinting of FT-IR spectroscopy combined with multivariate analysis. Whole cell extracts from leaves of 19 identified Zoysia japonica, 6 identified Zoysia sinica, and 38 different unidentified Zoysia species were subjected to Fourier transform infrared spectroscopy (FT-IR). PCA (principle component analysis) and PLS-DA (partial least square discriminant analysis) from FT-IR spectral data successfully divided the 25 identified turf grasses into two groups, representing good agreement with species identification using molecular markers. PC (principal component) loading values show that the $1,100{\sim}950cm^{-1}$ region of the FT-IR spectra are important for the discrimination of Zoysia species. A dendrogram based on hierarchical clustering analysis (HCA) from the PCA and PLS-DA data of turf grasses showed that turf grass samples were divided into Zoysia japonica and Zoysia sinica in a species-dependent manner. PCA and PLS-DA from FT-IR spectral data of Zoysia species identified and unidentified by molecular markers successfully divided the 49 turf grasses into Z. japonica and Z. sinica. In particular, PLS-DA and the HCA dendrogram could mostly discriminate the 47 Z. japonica grasses into two groups depending on their origins (mountainous areas and island area). Considering these results, we suggest that FT-IR fingerprinting combined with multivariate analysis could be applied to discriminate between Zoysia species as well as their geographical origins of various Zoysia species.

Dynamics of the Community of Phytoplankton and Periphytic Algae on Reed in the Shihwa Constructed Wetland (시화호 인공습지의 식물 플랑크톤과 갈대 부착조류 군집의 동태)

  • Kim, Yong-Jae;Kwun, Soon-Kuk
    • Korean Journal of Environmental Agriculture
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    • v.23 no.1
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    • pp.59-67
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    • 2004
  • The Shihwa constructed wetland was established for the treatment of severely polluted water from Banwoul, Donghwa and Samhwa streams. This study was focused on investigating dynamics of phytoplankton communities in open waters and periphytic algae on reed (Phragmites communis) planting area at 5 stations from October 2001 to July 2002. The concentration of T-N and T-P of inlet stations from the streams were decreased by flowed through the wetland. However, the TN/TP ratios at all stations were shown as a little over 16 indicating that the T-P will play some role as a limitation factor. Phytoplankton communities were identified as a total 413 taxa which were composed of 375 species, 21 varieties, 2 forma and 15 unidentified species. Standing crops of phytoplankton communities and chlorophyll-a concentrations ranged from $330{\sim}36,420\;cells/mL$ and $2.5{\sim}170.7\;{\mu}g/L$ respectively, and showed the decreasing tendency after flowing through the wetland at almost all stations. Dominant species were 14 taxa at all stations which were Euglena oblonga and Synura spinosa etc. Periphytic algae on the reed were a total 329 taxa which were composed of 295 species, 13 varieties, 3 forma and 17 unidentified species. The species numbers were recorded in order of Chlorophyceae-Bascillariophyceae-Cyanophyceae-Euglenophyceae-Chrysophyceae. The relative abundance (%) was showed a seasonal variation from Cyanophyceae to Bascillariophyceae and to Chlorophyceae. Chlorophyll-a concentrations, ie. the biomass of periphytic algae were ranged from $5.5{\sim}363.8\;mg/m^2$. Dominant species were 16 taxa which were Lyngbya angusta of Cyanophyceae in the early investigation, and were changed to Stigeoclonium lubricum of Chlorophyceae, and Nitzschia plea of Bacillariophyceae etc. in the late. Species number, standing crops and chlorophyll-a concentrations of phytoplankton and perphytic algae were shown higher values at the inlet stations than the stations after flowing through the wetland.

Notes on the Benthic Macrofauna During September 1997 Namdaecheon Estuary, Gangneung, Korea (강릉 남대천 하구역의 1997년 9월중 대형저서동물의 분포패턴)

  • 홍재상;서인수;윤건탁;황인서;김창수
    • Korean Journal of Environmental Biology
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    • v.22 no.2
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    • pp.341-350
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    • 2004
  • We examined estuarine macrobenthos in Namdaecheon estuary, Gangneung, Korea, September 22, 1997. A total of 56 species were found together with an abundance of 378 individuals $m^{-2}$/ and a biomass of 20.79 gWWt $m^{-2}$. The major dominant species were unidentified oligochaete followed by different species of polychaetes Hediste japonica, Rhynchospio glutaea, Poecilochaetus trilobatus, Scoloplos armiger, Spiophanes bombyx and a talitrid amphipod Platorchestia crassicornis. The study area was divided into two different groups of stations and species, based on the q -mode and r-mode clustering analysis. In case of q-mode, there are two groups: one is a marine station group and the other is estuarine group. The r-mode clustering analysis showed the two main communities as follows; 1) Marine species group occurred only at station 7, 8 and 9 and 2) Estuarine species group composed of the species present at station 1 to 6 and 10 to 15. In the number of species, the estuarine station group includes 13 species, whereas 43 species were presented in the marine station group. Total macrofaunal abundance and total biomass were higher in the marine station group than those in the estuarine group. The species diversity index was also high in the marine group (> 2), whereas it was less than ca. 1 in the estuarine group.

Seasonal variations in species composition by the stow nets and the stow net on boat fisheries in the Han River Estuary, Korea (한강 하구 해역에서 개량안강망 및 해선망으로 어획된 수산생물의 계절별 종 조성)

  • Oh, Taeg Yun;Lee, Jae Bong;Seo, Young Il;Lee, Jong Hee;Choi, Jung Hwa;Kim, Jung Yun;Lee, Dong Woo
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.48 no.4
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    • pp.452-468
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    • 2012
  • Marine organisms were collected by the stow nets and the stow net on boat fisheries from April 2010 to November 2011 in the Han River Estuary and categorized as 126 species, 61 families, and 5 taxa. The species were consisted with 34 in Crustacea, 5 in Cephalopoda, 79 in Pisces, unidentified jellyfishes in Cnidaria, and Finless porpoise in Mammals. The major species were composed of fish and crustaceans in the Han River Estuary. The dominant species in Crustacea were Chinese ditch prawn (Palaemon gravieri), blue crab (Portunus trituberculatus), Ridgetail prawn (Exopalaemon carinicauda), and mantis crab (Oratosquilla oratoria), and those in Pisces were Korean anchovy (Coilia nasus), and Japanese grenadier anchovy (Coilia mystus). The length structures of the six dominant species have more than one mode in the Han River Estuary. It reflects that the species inhibit during a part of and/or whole lifetime in the Han River Estuary where they utilize as spawning and/or nursery grounds. Freshwater fishes were collected from station D where is the closest location to the Han River stream, and their appearances were well matched with the large amount of freshwater discharge due to flood periods. Principal components analysis (PCA) was carried out with species compositions and showed temporal and spatial differences by the variations of species.

Comparisons of Isozyme Patterns in Pythium Species and Application to Pythium Systematics (Isozyme을 이용한 Pythium species의 비교 및 Pythium systematics에의 이용)

  • Lee, Youn-Su
    • The Korean Journal of Mycology
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    • v.21 no.4
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    • pp.293-300
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    • 1993
  • For the enzymes AAT, GmDH, ME, GPI, LDH and IDH, nine, seven, four, nine, seven, and four different phenotypes, respectively, were observed. All six isolates of an unidentified sterile Pythium sp. isolated from field soil showed the same band positions for all six enzymes compared. These phenotypes were not similar to any of the known Pythium species. Two isolates of unknown Pythium species (145 and 299) showed the same band positions for all six enzymes. The phenotypes for all three unknown Pythium spp. were different from the other species in the experiment. Five isolates of P. heterothallicum showed the same band positions for all enzymes compared except one enzyme, lDH. Two isolates of P. torulosum showed the same band petitions for enzymes AAT, GmDH and ME, and three isolates of P. totulosum showed the same positions for enzymes GPI, LDH, and IDH. Single isolates of P. spinosum and P. irregulare showed the same band positions for enzymes AAT, GmDH and GPI. In conclusion, sterile types of Pythium species showed 100% similarities among themselves but did not show any similarity with all isolates of P. heterothallicum and P. spinosum isolate, and showed very low similarities with other isolates in general except with unknown Pythium isolate 306. Similarity levels between different species were low in general with few exceptions.

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