Acoustic tweezers represent an exceptionally versatile and adaptable collection of instruments that harness the intrinsic power of sound waves to manipulate a wide spectrum of bioparticles, ranging from minuscule extracellular vesicles at the nanoscale to more substantial multicellular organisms measuring in millimeters. This field of research has witnessed remarkable progress over the course of the past few decades, primarily in the domain of Single Beam Acoustic Tweezers (SBAT) which utilizes a single element transducer for its operation. Initially conceived as a method for particle trapping, SBAT has since evolved into an advanced platform capable of achieving precise translation of cells and organisms. Recent groundbreaking advancements have significantly enhanced the capabilities of SBAT, unlocking new functionalities such as particle/cell separation and controlled deformation of single cells. These advancements have propelled SBAT to the forefront of bioparticle/cell manipulation, gathering attention within the scientific community. This review explores the core principles of SBAT and how sound waves affect bioparticles/cells. We aim to build a strong conceptual foundation for understanding advancements in this field by detailing its principles and methodologies.
For the purpose of possibility study on development of an acoustic tweezer using standing waves and very high frequency ultrasound focused beams, a system which can manipulate the position of particles in water has been constructed. It can move the particles to near focal point of a focused beam by the radiation force of standing waves, and then the particles would be trapped by the radiating force of the focused beam. The results show that micro sphere particles were trapped well at nodes of the standing waves and their position can be easily manipulated by frequency control. And, even though the radiation force by single focused beam pushes a particle away from the transducer, two focused confronted beams can trap it at near center.
Receive dynamic focusing with an array transducer can provide near optimum resolution only in the vicinity of transmit focal depth. A customary method to increase the depth of field is to combine several beams with different focal depths, with an accompanying decrease in the frame rate. In this Paper. we Present a simultaneous multiple transmit focusing method in which chirp signals focused at different depths are transmitted at the same time. These chirp signals are mutually orthogonal in a sense that the autocorrelation function of each signal has a narrow mainlobe width and low sidelobe levels. and the crossorelation function of any Pair of the signals has values smaller than the sidelobe levels of each autocorrelation function. This means that each chirp signal can be separated from the combined received signals and compressed into a short pulse. which is then individually focused on a separate receive beamformer. Next. the individually focused beams are combined to form a frame of image. Theoretically, any two chirp signals defined over two nonoverlapped frequency bands are mutually orthogonal In the present work. however, a tractional overlap of adjacent frequency bands is permitted to design more chirp signals within a given transducer bandwidth. The elevation of the rosscorrelation values due to the frequency overlap could be reduced by alternating the direction of frequency sweep of the adjacent chirp signals We also observe that the Proposed method provides better images when the low frequency chirp is focused at a near Point and the high frequency chirp at a far point along the depth. better lateral resolution is obtained at the far field with reasonable SNR due to the SNR gain in Pulse compression Imaging .
Purpose: Renal stones are common and typically arise within the collecting system. The renal sinus are contains the collection system, the renal vessels, lymphatcs, fat, and fibrous tissue. Because of the compression of all the large echoes in signal processing, the echo from the renal stone generally cannot be distinguished from large echoes emanating from normal structures of the renal sinus. Use of ultrasonography has been difficult for detecting small renal stone without posterior shadowing and chemical composition of stone. The aim of study was measuring for posterior acoustic shadowing to a stone for various scan parameter and it examines a help in renal stone diagnosis. Material & Methods: The stone was place on sponge examined in a water bath with a 3.5MHz or 7.5MHz transducer(LOGIQ 400, USA). First, tested a variety of gain. Second, tested a variety of dynamic range. Third, tested a variety of focal zone. Fourth, measuring of the echo level for low and high frequency for depth. Results: 1) Average echo level was 98 for low total gain(10 dB) and was 142 for high total gain(40 dB). Posterior acoustic shadowing of renal stone was clear for low gain. 2) Average echo level was 129 for low dynamic range(42 dB) and was 101 for high dynamic range(72 dB). Posterior acoustic shadowing of renal stone was clear for high dynamic range. 3) When stone is in focal zone of transducer, definite posterior acoustic shadow is identified. 4) Stone was clear appeared for high frequency(7.5 MHz) than low frequency(3.5 MHz) and it is not distorted. Conclusion: The demonstration of an posterior acoustic shadow of renal stone dependents on several technical factors such as gain, dynamic range, focus, and frequency. This various factors are a help in renal stone diagnosis.
Purpose : To evaluate and compare the accuracy of magnetic resonance imaging (MRI) and ultrasound (US) for detection and estimation of invasion depth of colorectal carcinoma (CRC) by correlation with histopathologic findings in vitro, and to find out the best MR pulse sequence for accurate delineation of tumor from surrounding normal tissue. Materials and Methods: Resected specimens of CRC from 45 patients were examined about tumor detectability and invasion depth of US using high frequency (5-17 MHz) linear transducer in a tube filled with normal saline and MRI in a 8-channel quadrate head coil. The institutional review board approved this study and informed consent was waived. MRI with seven pulse sequences of in- and out-of-phases gradient echo T1 weighted images, fast spin echo T2 weighted image and its fat suppression image, fast imaging employing steady-state acquisition (FIESTA) and its fat suppression image, and diffusion weighted image (DWI) were performed. In each case, both imaging findings of MRI and US were evaluated independently for detection and estimation of invasion depth of tumor by consensus of two radiologists and were compared about diagnostic accuracy according to the histopathologic findings as reference standard. Seven MR pulse sequences were evaluated on the point of accurate delineation of tumor from surrounding normal tissue in each specimen. Results: In specimens of CRC, both imaging modalities of MRI (91.1%) and US (86.7%) showed relatively high diagnostic accuracy to detect tumor and evaluate invasion depth of tumor. In early CRC, diagnostic accuracy of US was 87.5% and that of MRI was 75.0%. There was no statistically significant difference between two imaging modalities (p > 0.05). The best pulse sequence among seven MR sequences for accurate delineation of tumor from surrounding normal tissue in each specimen of CRC was fast spin echo T2 weighted image. Conclusion: MRI and US show relatively high diagnostic accuracy to detect tumor and evaluate invasion depth of resected specimen of CRC. The most excellent pulse sequence of MRI for accurate delineation of tumor from surrounding normal tissue in CRC is fast spin echo T2 weighted image.
Purpose : To evaluate and compare the diagnostic accuracy of MRI and ultrasound(US) for estimation of invasion depth of gastric carcinoma by correlation with histopathologic findings in vitro and to find out the best MR pulse sequence for detection and accurate delineation of tumor. Materials and Methods : Resected specimen of total or subtotal gastrectomy from 53 patients with gastric carcinoma were done of imaging studies of MRI and US. And US was examined by using high frequency linear transducer for tumor invasion depth by a radiologist. In each case, both imaging findings of MRI and US were evaluated independently for tumor detection and invasion depth by consensus of two radiologists and were compared the diagnostic accuracy between two imaging modalities according to the histopathologic findings. MR imaging with five MR pulse sequences, spin echo T1 and in- and out-of phase gradient echo T1 weighted images, FSE and SSFSE T2 weighted images, were performed. Five MR pulse sequences were evaluated and compared on the point of detection and accurate distinction of tumor from surrounding normal tissue. Results : In EGC, diagnostic accuracy of US(77%) was superior than that of MRI(59%) but no statistically significant difference was noted between two imaging modalities(p=0.096). In AGC, both imaging modalities of MRI and US showed relatively high diagnostic accuracy as 97% and 84% respectively. Diagnostic accuracy of MRI was statistically better than that of US at the significant level(p<0.001). The best MR pulse sequence among five in each specimen was FSE T2WI(75.5%, 40/53) in both EGC and AGC. In AGC, FSE T2WI showed excellent imaging quality by showing very high ratio (93.5%, 29/31) of accurate delineation of tumor. Conclusion : MRI and US show relatively high diagnostic accuracy in the evaluation of tumor invasion depth of resected specimen in AGC. The most excellent pulse sequence of MRI for the evaluation of tumor invasion depth is FSE T2WI on the point of detection and accurate delineation of tumor in both EGC and AGC.
Lee Kichang;Jung Joohyun;Oh Sunkyoung;Jeong Yucheol;Lim Changyun;Yoon Junghee;Choi Mincheol
Journal of Veterinary Clinics
/
v.22
no.3
/
pp.186-189
/
2005
For the assessment of the clinical application of histogram on internal parenchymal organs, ultrasonography with a multi-frequency transducer was taken. We scanned in the region of right cranial abdomen for both liver and right kidney, and left cranial abdomen for liver, spleen and left kidney in 9 normal Beagle dogs. The data from histogram examined in a region of interest centered on each picture element of B-mode images at the same depth were compared among liver, renal cortex, spleen, cortex and medulla of each kidney. The right renal cortex showed significantly lower echogenicity than parenchyma of liver by $15{\%}$. Spleen was more echogenic than the cortex of the left kidney by $23{\%}$, and liver was more echogenic than the left renal cortex by $30{\%}$. Renal cortex was more echogenic than medulla by $47{\%}$ and $65{\%}$ on the right and left side, respectively (p<0.05). The mean (${\pm}SD$) values calculated echogenicity were $46.2{\pm}12.3\;(95\%$ confidential interval (CI), 41.0 to 55.0) and $53.4{\pm}12.1\;(95\%$ CI, 47.0 to 55.1) in in the right renal cortex and liver parenchyma, $65.0{\pm}11.8\;(95\%$ CI, 57.9 to 71.0) and $51.0{\pm}16.9\;(95\%$ CI, 42.8 to 54.1) in splenic parenchyma and renal cortex. And the mean values calculated echogenicity were $65.0{\pm}10.15\;(95\%$ CI, 60.1 to 71.5) and $52.0{\pm}9.4\;(95\$ CI, 43.8 to 60.3) in liver parenchyma and the left renal cortex, $54.5{\pm}18.3\;(95\%$ CI, 40.1 to 62.8) and $35.0{\pm}16.2\;(95\%$ CI, 24.2 to 43.6) in the left renal cortex and medulla. And the mean values calculated echogenicity were $55.0{\pm}14.4\;(95\%$ CI, 47.3 to 61.7) and $40.0{\pm}13.2\;(95\%$ CI, 34.3 to 46.7) in the right renal cortex and medulla, respectively. In addition, the echogenicity ratios were $0.86{\pm}0.11$ between the right renal cortex and liver parenchyma, $1.37{\pm}0.47$ between spleenic parenchyma and the left renal cortex, $1.30{\pm}0.19$ between liver parenchyma and the left renal cortex. All the values measured showed significant different (p<0.05). Ultrasound histogram is simple, useful and feasible to evaluate the sonographic architecture of the internal organs such as liver, spleen and kidney, quantitatively.
For application to several MHz photoacoustic imaging systems, a needle hydrophone was designed and fabricated by using PMN-PZT piezoelectric single crystal, and its characteristics were evaluated through comparison with a commercial PVDF(Polybinylidene Fluoride) hydrophone of which receiving sensitivity is known. The simulation using the KLM model results show that the peak receiving impulse response for $50{\Omega}$ terminating impedance of the fabricated hydrophone is -261.6 dB re $1V/{\mu}Pa$ and the frequency response is relatively flat over 2 ~ 12 MHz with fluctuation less than 5 dB. The measurement results using tone burst signals also show that it has higher (ave. 10.9 dB) sensitivity than the commercial hydrophone in 2 ~ 8 MHz, and the receiving sensitivity of $-255.8{\pm}2.8$ dB re $1V/{\mu}Pa$ was measured for the fabricated hydrophone. In addition, it is known that the photoacoustic signals and the image of a hair obtained by a mechanical scanned photoacoustic imaging system with the fabricated hydrophone were bigger and better than those obtained with the commercial hydrophone.
Sector scanner which has a conical end is used to image through the intercostal space because heart is protected by the ribs. Cardiac data published all around the world were also obtained by sector scanner. Although scanners being used in every small animal practice and animal hospital at college in Korea include convex ape and linear type, linear type is not appropriate f3r cardiac scan because of a wide contact surface. The purpose of this study is to establish ultrasonographic images of normal cardiac structures by measuring shape, size of reflectable cardiac structure according to restraint position in scanning normal heart of the puppies with 6.5 MHz convex scanner(SonoAce 4500, Medison, Korea) used in our veterinary teaching hospital, Seoul national university. Seventeen male and female puppies considered having healthy hear by X-ray and clinical examination are used feom April to July 1994. Scanning point selection of probe head and the distinction of imaged cardiac structures were accomplished by necropsy and cardiac scanning performed through thoracotomy under general anesthesia. At 10 o'clock position of transducer(at an angle of 30$^{\circ}$ between imaginary line from elbow joint to 3rd sternum and probe head, 60$^{\circ}$ from body surface, 4th intercostal space of right thorax) with the marker of scanner toward the head of dogs right atrium, left atrium and left ventricle were observed in 2, 3, 4, 5 intercostal space(2cm from the sternum) of experimental dog positioned ventrodorsally under general anesthesia. Under these conditions, the numerical values of imaged diastolic hear are as follows : the distance from skin to apex(mean$\pm$S.D) 47.53$\pm$6.94mm, thickness of left ventricular wall 6.00$\pm$1.60mm, length of left ventricle 16.27$\pm$5.31mm, width of left ventricle 15,33$\pm$4.25mm, length of left atrium 12.33$\pm$3.82mm, width of left atrium 11. 33$\pm$3.94mm, length of right atrium 1.00$\pm$2.41mm, width of right atrium 11.21$\pm$2.76mm and the area of left ventricle 270.92$\pm$109.81mm$^2$, area of left atrium 98.00$\pm$41.08mm$^2$, area of right atrium 62.75$\pm$21.04mm$^2$.
Since measuring the size of kidney with sonography becomes an important index for diagnosis, treatment, and prognostic prediction in kidney disease, the accurate measurement and evaluation on this are clinically very important. Accordingly, the purpose of this study was to increase reproducibility and objectivity in measuring the size of kidney by enumerating factors that have an impact for measurement. It targeted 44 adults in Korea at the age of 21-27. It measured in order for both kidneys to be seen most largely while changing a subject-examiner's position in a state of fasting for 8 hours and a transducer's approaching direction. It compared a size of kidney by measuring, respectively, with the same method in 30 minutes and in 1 hour after drinking water in 700-1,000cc. In case of the lateral approach scan in decubitus position, the average length of the kidney both to the right and the left and the deviation of measurement to be the largest. In NPO(None Per Oral) state, the average length in the right kidney was 10.19cm, and the average length in the left kidney was 10.33cm. In 60 minutes after taking moisture, the average length in the right kidney was 10.94cm, and the average length in the left kidney was 11.13cm. In comparing the average length of the kidney in NPO state and its average length in 60 minutes after taking moisture, the size swelled by 7.3% for the length in the right kidney and by 7.7% in the left, thereby having been indicated to be statistically significant(P<0.003). The measurement in a size of kidney by using ultrasound may be measured differently depending on a patient's state of taking moisture and a transducer's approaching direction. It is thought that when the measurement in a size of kidney is especially important clinically, the intake and intake time in moisture need to be considered and that measuring with the posterior approach in prone position is a good method aiming to increase reproducibility in measuring length of the kidney.
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