• Fisheries and Aquatic Sciences
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• 제21권11호
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• pp.34.1-34.13
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• 2018

Sexual maturity ($L_{50}$), the length at which 50% of fish in a size class are mature, is a key aspect of domestication of new fish species because it guides the procedure for identification of appropriate broodstock size for artificial spawning. In this study, the $L_{50}$ was determined for 1083 Barbus altianalis samples obtained from Lake Edward and the Upper Victoria Nile. Gonads of freshly killed samples were examined macroscopically and verified with standard histological procedures for the maturation stages that were used to determine $L_{50}$. Oocytes and spermatogenic cell sizes were compared for fish obtained from both water bodies. Results indicated that there were no variations in macro gonad features observed for fish from Lake Edward and Upper Victoria Nile. Similarly, there were no significant differences in oocyte sizes (P > 0.05) between the two populations but significant differences in spermatogenic cell sizes were noted (P < 0.05) except for spermatozoa (P > 0.05). This however did not suggest peculiar differences between the two populations for staging the gonads. Consequently, no staging variations were suggested for both populations in determination of $L_{50}$. Sexual maturity was found in the same class size of fork length (FL) 20-24.9 cm and 35-39.9 cm for males and females from both water bodies, respectively. At this FL, however, males were too small, and for good selection of vigor broodstocks for spawning and conservation purposes, they are better picked from class size of 30-34.9 cm FL and above. These findings were crucial for integration of appropriate breeding size in spawning protocol by farmers and fisheries scientists conserving wild B. altianalis populations.

• 농촌지도와개발
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• 제20권4호
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• pp.1045-1078
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• 2013

본 연구는 아프리카 지역의 농업기술보급체계를 진단하고, 보급체계의 발전방향 및 개발원조 전략을 제시함으로써 ODA(Official Development Assistance)를 통한 아프리카 농업기술전수의 효과성 제고에 기여하고자 한다. 기존의 대(對)아프리카 농업원조는 아프리카의 농업이 내포하고 있는 '다양성'과 '복잡성'의 가치를 간과한 채 진행되어 왔으며, 막대한 원조금액의 기대와 달리, 아프리카의 농업발전은 답보 상태에 놓여 있다. 본 연구는 농업기술보급체계 및 ODA사업 전략의 아프리카화(Africanization) 논의를 제기하고 있으며, 설문조사 일변도의 미시적 시각에서 나아가 사례국가별 농업기술보급체계의 거시적 특성을 분석하고 있다. 연구 결과, 사례국가의 기술보급체계는 조직과 기능에 따라 상이한 특성을 지니고 있으며, 이에 기술보급체계의 주체별 역할 세분화와 기능별 역량 분석 내용 또한 차별적으로 구성되었다. 에티오피아의 경우, 정부조직의 규모별로 하향식 농업기술보급체계가 구축되어 있으며, 전달체계의 최종행위자인 농민집단의 참여가 확대되고 있다. 그러나 여전히 기술전달을 통한 생산성 향상 등의 기능보다는 농가경영적인 측면에 치중되어 있어, 전달 기술의 효과적인 활용이 결여되어 있는 실정이다. 반면 우간다의 사례에서는 전 기관을 포괄하는 참여적 지도체계가 구축되어 있으나, 기술지도의 시급성에도 불구하고 지도 역할보다는 연구 기능의 확대에 투자가 집중되어 있음이 규명되었다. 상기 연구결과는 현 농업기술보급체계의 개선과 이를 토대로 한 농업기술전수사업의 수행이 아프리카 농업 농촌 ODA사업의 효과성 제고와 농업침체의 문제를 해결하기 위한 필요조건임을 논증하고 있다.

• Journal of Veterinary Science
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• 제22권4호
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• pp.43.1-43.10
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• 2021

Background: The H5 avian influenza viruses (AIVs) of clade 2.3.4.4 circulate in wild and domestic birds worldwide. In 2017, nine strains of H5N6 AIVs were isolated from aquatic poultry in Xinjiang, Northwest China. Objectives: This study aimed to analyze the origin, reassortment, and mutations of the AIV isolates. Methods: AIVs were isolated from oropharyngeal and cloacal swabs of poultry. Identification was accomplished by inoculating isolates into embryonated chicken eggs and performing hemagglutination tests and reverse transcription polymerase chain reaction (RT-PCR). The viral genomes were amplified with RT-PCR and then sequenced. The sequence alignment, phylogenetic, and molecular characteristic analyses were performed by using bioinformatic software. Results: Nine isolates originated from the same ancestor. The viral HA gene belonged to clade 2.3.4.4B, while the NA gene had a close phylogenetic relationship with the 2.3.4.4C H5N6 highly pathogenic avian influenza viruses (HPAIVs) isolated from shoveler ducks in Ningxia in 2015. The NP gene was grouped into an independent subcluster within the 2.3.4.4B H5N8 AIVs, and the remaining six genes all had close phylogenetic relationships with the 2.3.4.4B H5N8 HPAIVs isolated from the wild birds in China, Egypt, Uganda, Cameroon, and India in 2016-2017, Multiple basic amino acid residues associated with HPAIVs were located adjacent to the cleavage site of the HA protein. The nine isolates comprised reassortant 2.3.4.4B HPAIVs originating from 2.3.4.4B H5N8 and 2.3.4.4C H5N6 viruses in wild birds. Conclusions: These results suggest that the Northern Tianshan Mountain wetlands in Xinjiang may have a key role in AIVs disseminating from Central China to the Eurasian continent and East African.