• Title/Summary/Keyword: UV-B radiation

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Inhibition of Photooxidation by Breakdown of ChlorophyII in Oil Model System Using Gamma Irradiation (감마선 조사된 유지 model system의 chlorophyII 제거 및 광산화 억제)

  • Lee, Kyong-Haeng;Jo, Cheo-Run;Yook, Hong-Sun;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.33 no.6
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    • pp.693-699
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    • 2001
  • ChlorophyII destruction by irradiation in linoleic acid in methanol system was studied. ChlorophyII b standard (3 ppm) was added into methanol solution containing 1% of linoleic acid, and the sample was irradiated up to 20 kGy with or without nitrogen gas-bubbling. The content of chlorophyII b was analyzed by HPLC, and Hunter color value and UV-visible spectra were analyzed during 6 hr of photooxidation. The content of chlorophyll was reduced by irradiation and completely destroyed at 2.517 kGy. The model system with nitrogen gas-bubbling did not develop the lipid oxidation after irradiation or photooxidation at 20 kGy UV-visible spectra showed decreasing values of optical density by increase of irradiation doses, supporting the destruction of chlorophyII b. The results indicate that irradiation technology can be applied to reduce or eliminate the residual chlorophyII and to prolong the shelf-life of oil products.

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Classification of Biological Effect of 1,763 MHz Radiofrequency Radiation Based on Gene Expression Profiles

  • Im, Chang-Nim;Kim, Eun-Hye;Park, Ae-Kyung;Park, Woong-Yang
    • Genomics & Informatics
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    • v.8 no.1
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    • pp.34-40
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    • 2010
  • Radiofrequency (RF) radiation might induce the transcription of a certain set of genes as other physical stresses like ionizing radiation and UV. To observe transcriptional changes upon RF radiation, we exposed WI-38, human lung fibroblast cell to 1763 MHz of mobile phone RF radiation at 60 W/kg of specific absorption rate (SAR) for 24h with or without heat control. There were no significant changes in cell numbers and morphology after exposure to RF radiation. Using quantitative RT-PCR, we checked the expression of three heat shock protein (HSP) (HSPA1A, HSPA6 and HSP105) and seven stress-related genes (TNFRSF11B, FGF2, TGFB2, ITGA2, BRIP1, EXO1, and MCM10) in RF only and RF/HS groups of RF-exposed cells. The expressions of three heat shock proteins and seven stress-related genes were selectively changed only in RF/HS groups. Based on the expression of ten genes, we could classify thermal and non-thermal effect of RF-exposure, which genes can be used as biomarkers for RF radiation exposure.

Topical or oral treatment of peach flower extract attenuates UV-induced epidermal thickening, matrix metalloproteinase-13 expression and pro-inflammatory cytokine production in hairless mice skin

  • Kwak, Chung Shil;Yang, Jiwon;Shin, Chang-Yup;Chung, Jin Ho
    • Nutrition Research and Practice
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    • v.12 no.1
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    • pp.29-40
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    • 2018
  • BACKGROUND/OBJECTIVES: Ultraviolet radiation (UV) is a major cause of skin photoaging. Previous studies reported that ethanol extract (PET) of Prunus persica (L.) Batsch flowers (PPF, peach flowers) and its subfractions, particularly the ethylacetate (PEA) and n-butanol extracts (PBT), have potent antioxidant activity and attenuate the UV-induced matrix metalloproteinase (MMP) expression in human skin cells. In this study, we investigated the protective activity of PPF extract against UV-induced photoaging in a mouse model. MATERIALS/METHODS: Hairless mice were treated with PET or a mixture of PEA and PBT either topically or orally along with UV irradiation. Histological changes and biochemical alterations of mouse skin were examined. Major phenolic compounds in PPF extract were analyzed using an ACQUITY UPLC system. RESULTS: The overall effects of topical and oral treatments with PPF extract on the UV-induced skin responses exhibited similar patterns. In both experiments, the mixture of PEA and PBT significantly inhibited the UV-induced skin and epidermal thickening, while PET inhibited only the UV-induced epidermal thickening. Treatment of PET or the mixture of PEA and PBT significantly inhibited the UV-induced MMP-13 expression, but not type I collagen expression. Topical treatment of the mixture of PEA and PBT with UV irradiation significantly elevated catalase, superoxide dismutase (SOD) and glutathione-peroxidase (GPx) activities in the skin compared to those in the UV irradiated control group, while oral treatment of the mixture of PEA and PBT or PET elevated only catalase and SOD activities, but not GPx. Thirteen phytochemical compounds including 4-O-caffeoylquinic acid, cimicifugic acid E and B, quercetin-3-O-rhamnoside and kaempferol glycoside derivatives were identified in the PPF extract. CONCLUSIONS: These results demonstrate that treatment with PET or the mixture of PEA and PBT, both topically or orally, attenuates UV-induced photoaging via the cooperative interactions of phenolic components having anti-oxidative and collagen-protective activities.

Extraction of Caffeine from Spent Coffee Grounds and Oxidative Degradation of Caffeine (커피 찌꺼기의 카페인 용출 및 산화분해 특성)

  • Shin, MinJeong;Kim, Young-Hun
    • Journal of Environmental Science International
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    • v.27 no.12
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    • pp.1205-1214
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    • 2018
  • During the past few decades, significant increase in the consumption of coffee has led to rapid increase in the production of coffee waste in South Korea. Spent coffee waste is often treated as a general waste and is directly disposed without the necessary treatment. Spent Coffee Grounds (SCGs) can release several organic contaminants, including caffeine. In this study, leaching tests were conducted for SCGs and oxidative degradation of caffeine were also conducted. The tested SCGs contained approximately 4.4 mg caffeine per gram of coffee waste. Results from the leaching tests show that approximately 90% of the caffeine can be extracted at each step during sequential extraction. Advanced oxidation methods for the degradation of caffeine, such as $UV/H_2O_2$, photo-Fenton reaction, and $UV/O_3$, were tested. UV radiation has a limited effect on the degradation of caffeine. In particular, UV-A and UV-B radiations present in sunlight cause marginal degradation, thereby indicating that natural degradation of caffeine is minimal. However, $O_3$ can cause rapid degradation of caffeine, and the values of pseudo-first order rate constants were found to be ranging from $0.817min^{-1}$ to $1.506min^{-1}$ when the ozone generation rate was $37.1g/m^3$. Additionally, the degradation rate of caffeine is dependent on the wavelength of irradiation.

A Theoretical and Experimental Study for the Design of Solar Air Heaters Using Porous Material (다공성 물질을 이용한 공기용 태양열 집열기의 설계를 위한 이론 및 실험적 연구)

  • Hwang, Yong-Ha;Park, Seung-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.20 no.1
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    • pp.336-345
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    • 1996
  • A theoretical and experimental study was conducted in order to design highly efficient solar air heaters using porous material. Radiative characteristics of glass windows and porous absorbing media were correlated through spectral transmittances measured by the UV-Visible and the FT-IR spectrometers. Using those characteristics the efficiencies of collectors were numerically calculated with the use of the two-flux radiation model. Based on the theoretical results, an experimental solar collector was designed and constructed. Experimental results under various conditions show that the daily efficiencies are over 60% in general and agree well with the theoretically calculated ones.

Study on Quality Criteria for Transparent Soundproof Panels(2) - Sound Insulation Characteristics and Evaluation of Weatherability (투명방음판의 품질기준 설정에 관한 연구(2) - 음향 특성 및 내후성 평가)

  • Chang, Taesun;Kim, Chulhwan;Hwang, Cheolho
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.22 no.11
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    • pp.1099-1105
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    • 2012
  • Transparent noise barrier panels have the advantages of transmitting light to nearby residents and allowing drivers to orientate themselves by providing views of the surrounding area. To develop guidelines on quality criteria for transparent soundproof panels, their sound transmission loss and weatherability were experimentally investigated. In Korea, the sound transmission loss of noise barrier panels should be more than 25 dB at 500 Hz and 30 dB at 1000 Hz. The transmission loss tests of transparent panels were performed in accordance with KS F 2808. Typically, plastic materials suffer from color changes when exposed to ultraviolet(UV) light over extended periods. Therefore, weathering of plastics is one of the most important properties for outdoor applications. Protection against UV radiation and weathering is usually accomplished by using UV stabilizer additives or coatings. Transparent materials for soundproof panels were examined through accelerated weathering tests and their weathering resistances were evaluated from changes in yellowness index.

The Anti-melanogenic Effect of Whitening Agent Containing Arbutin and ${\beta}-Glucosidase$ (알부틴과 베타-글루코시다제를 함유하는 미백 화장품의 항멜라닌 효과)

  • Lew Bark-Lin;Ryou Ji-Ho;Lee Mu-Hyoung
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.3 s.47
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    • pp.415-418
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    • 2004
  • Arbutin is a glycosylated hydroquinone found at high concentration in certain plants capable of surviving extreme and sustained dehydration. It has been reported to have an inhibitory effect of melanogenesis and to be hydrolyzed easily to yield glucose and hydroquinone by ${\beta}-glucosidase.$ While hydroquinone also has an anti-melanogenic effect, however, is carefully used as a topical whitening agent because of side effects. The present study was undertaken to examine the inhibitory effect of an whitening agent containing arbutin and ${\beta}-glucosidase$ on UV radiation induced pigmentation in human skin. Experimental subjects were UVB-irradiated on the back. UVB-irradiated areas were assigned to three groups: arbutin and ${\beta}-glucosidase$ treated group, vehicle control, and no-application control. Arbutin and ${\beta}-glucosidase$ treatment inhibited pigmentation by 50.17 percent, compared with the controls (N : 10: P<0.05). These results suggest that the whitening agent containing arbutin and ${\beta}-glucosidase$may be used as an agent to inhibit melanin formation induced by UV radiation.

Activities of Extract from Leonurus sibiricus Against UVB-Damage in HS68 Cell (자외선 B로 산화적 손상이 유도된 HS68 세포에 익모초 추출물의 효능 평가)

  • Kim, Bo-Ae
    • The Korea Journal of Herbology
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    • v.32 no.5
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    • pp.13-18
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    • 2017
  • Objectives : The aim of this study is to investigate anti-inflammation of Leonurus sibiricus methanol extract against UVB-damage in fibroblast. The skin is continuously exposed to damage from environmental stresses. UV radiation causes a variety of biological effects especially on the skin, including inflammation and photoaging. Methods : In this study, we tried to search for Leonurus sibiricus which exhibit protective activities against UVB-induced cytotoxicity and oxidative cell death, NO and $PGE_2$ production. HS68 cells were exposed to UVB ($120mJ/cm^2$) and treated with various concentrations (0, 0.25, 0.5, 1, 2, 4, $8mg/m{\ell}$) of Leonurus sibiricus methanol extract for additional 24 h. Intracellular reactive oxygen species (ROS) levels generated by UV radiation were detected using a spectrofluorometer after DCF-DA staining. Also, HS68 cells were irradiated with UVB and then treated with Leonurus sibiricus methanol extract for 12 h. The lipid peroxidation was assayed by measuring the levels of 8-isoprostane secreted into the culture medium. Results : UVB-induced cytotoxicity and cell death were effectively suppressed by treatment of Leonurus sibiricus aqueous methanol extracts. Oxidative cell damage was mediated $PGE_2$ in UVB-induced HS68 fibroblast cell, which was significantly inhibited by treatment with Leonurus sibiricus extracts. Also, the protective effect of these extract seemed to be mediated by inhibited intracellular ROS generation and lipid peroxidation in dose-dependent manner. Conclusion : These results suggest that Leonurus sibiricus aqueous methanol extracts may have anti-aging effects new functional materials against oxidative UVB stress-mediated skin damages.

Color Change of Major Wood Species Planted in Indonesia by Ultraviolet Radiation (자외선 조사에 의한 인도네시아 주요 조림수종 목재의 재색변화)

  • Park, Se-Hwi;Jang, Jae-Hyuk;Qi, Yue;Hidayat, Wahyu;Hwang, Won-Joong;Febrianto, Fauzi;Kim, Nam-Hun
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.1
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    • pp.9-18
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    • 2016
  • This study was performed to understand wood durability to climate deterioration of planted Indonesian wood specie such as Albizia, Gmelina, Mangium and Mindi. Wood samples were exposed to indoor and outdoor condition. $L^*a^*b^*$ and Color changes (${\Delta}E*ab$) were determined by a spectrophotometer. As a result, color of all samples was changed more markedly by ultraviolet radiation. In indoor test with UV, brightness of wood specimens from four species was not changed and all samples were changed into more reddish and yellowish. In accelerated weathering test, all samples were bleached and changed into more greenish and blueish. In outdoor test, brightness of wood specimens decreased in Albizia and Gmelina and increased in Mangium and Mindi. All wood specimens in outdoor test were changed into more greenish and blueish. Albizia and Gmleina woods showed greater color change than those of Mangium and Mindi. Especially, color change of wood samples might be influenced greatly by moisture. In conclusion, wood color changed into more clearly by UV radiation. Therefore, exposing woods to UV radiation could be one of reasonable methods to improve wood quality on visual characteristic.

Effect of UV-C Irradiation on the Quality of Imported Dried Fish during Storage (UV-C 조사가 수입 건어포류의 저장 중 품질에 미치는 영향)

  • Kim, Ju-Yeon;Chun, Ho-Hyun;Song, Kyung-Bin
    • Food Science and Preservation
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    • v.15 no.6
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    • pp.922-926
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    • 2008
  • The effect of UV-C irradiation on microbial growth and the quality of imported dried filefish and octopus during storage was examined. Samples of imported dried filefish and octopus were irradiated at 0, 5, 10 and $20\;kJ/m^2$ and stored for 3 months at $20^{\circ}C$ or for 6 days at $4^{\circ}C$. Exposure times of 5 min 33 sec, 11 min 6 sec, and 22 min 12 sec were used. UV-C treatment of the imported dried filefish and octopus decreased the populations of aerobic bacteria, yeasts and molds in proportion to radiation dose. Compared to the control, total aerobic bacteria, and yeast and mold populations were significantly lower (1-2 log CFU/g) with UV-C treatment of $20\;kJ/m^2$. UV-C irradiation caused negligible changes in the Hunter color L, a and b values. These results indicate that UV-C irradiation could be useful in inhibiting microbial growth on imported dried fish without impairing quality during storage.