• 제목/요약/키워드: UV sensitivity

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Mapping of UV-B sensitive gene in Arabidopsis by CAPS markers (CAPS marker에 의한 Arabidopsis의 자외선 B 감수성 유전자 지도작성)

  • 박홍덕;김종봉
    • Journal of Life Science
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    • v.12 no.6
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    • pp.715-720
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    • 2002
  • A mutant Arabidopsis thaliana which is very sensitive to Ultraviolet-B(UV-B) radiation has been isolated by ethylmethane sulfonate(EMS) mutagenesis. Genetic cross proved the UV-sensitive gene(uvs) to segregate as a single Mendelian locus. For mapping of uvs, we crossed Arabidopsis thaliana Lansberg with uvs plant(Columbia), and made F2 plants by F1 selfcross. We designed 10 kinds of CAPS marker primers. Each primers amplifies a single mapped DNA sequence from uvs and Lansberg erecta ecotyres. Also identified was at least one restriction endonuclase for each of these PCR product that generates ecotype-specific digestion pattern. We got crossing over value of UB-sensitivity and each CAPS marker which located on different chromosome arm. The value of crossing over showed that uvs was linked to LFY3 which was on chromosome 5.

Electrochemical Properties of Dye-sensitized Solar Cells with Improving the Surface Structure (표면형상 변화에 따른 염료감응 태양전지의 전기화학적 특성)

  • Zhao, Xing Guan;Jin, En Mei;Gu, Hal-Bon
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.25 no.2
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    • pp.153-158
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    • 2012
  • We use UV(ultraviolet)-$O_3$ treatment to increase the surface area and porosity of $TiO_2$ films in dye-sensitized solar cells (DSSCs). After the UV-$O_3$ treatment, surface area and porosity of the $TiO_2$ films were increased, the increased porosity lead to amount of dye loading and solar conversion efficiency was improved. Field emission scanning electron microscopy images clearly showed that the nanocrystalline porosity of films were increased by UV-$O_3$ treatment. The Brunauer, Emmett, and Teller surface area of the $TiO_2$ films were increased from $0.71cm^2/g$ to $1.31cm^2/g$ by using UV-$O_3$ treatment for 20 min. Also, UV-$O_3$ treatment of $TiO_2$ films significantly enhanced their solar conversion efficiency. The efficiency of the films without treatment was 4.9%, and was increased to 5.6% by UV-$O_3$ treatment for 20 min. Therefore the process enhanced the solar conversion efficiency of DSSCs, and can be used to develop high sensitivity DSSCs.

Gene-Specific Repair of 6-4 Photoproducts in Trichothiodystrophy Cells

  • Nathan, Sheila;Van Hoffen, Anneke;Mullenders, Leon H.F.;Mayne, Lynne V.
    • BMB Reports
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    • v.32 no.6
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    • pp.554-560
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    • 1999
  • TTD1BI cells are non-hypersensitive to UV irradiation and perform normal genome repair of pyrimidine dimers but fail to excise 6-4 photoproducts and, concomitantly, are unable to restore RNA synthesis levels following UV irradiation. This pointed to a detect in gene-specific repair and this study was undertaken to examine repair of 6-4 photoproducts at the gene-level. The results indicated a defect in gene-specific repair of 6-4 photoproducts in active genes, although strand-specificity of 6-4 photoproduct removal was essentially similar to that of normal cells. These findings indicate that the near normal UV resistance of TTD1BI cells may be due to the inability of these cells to remove DNA lesions preferentially, as well as to the cells opting out of the cell cycle to repair damage before resuming replication.

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A Studies on Indirect Photometric Detection of Aliphatic Compounds by High-Performance Liquid Chromatography (고성능 액체 크로마토그래피에 의한 지방족 화합물의 간접 분광광도 측정에 관한 연구)

  • Sam-Woo Kang;Jeon-Tag Kang
    • Journal of the Korean Chemical Society
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    • v.33 no.4
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    • pp.406-412
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    • 1989
  • Indirect photometric detection of aliphatic compounds such as alcohols, ketones and esters was investigated in high-performance liquid chromatography, These samples possessing nonchromophore or weak chromophore could be indirectly detected by a UV detector with addition of UV absorbing, p-chlorophenol or p-nitrophenol in mobile phase as detection reagent, and submicrogram detection limits were achieved. Some mixtures of samples were also able to be separated and quantitated with good resolution and comparatively high sensitivity under optimum conditions.

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Design and construction of a new ultraviolet sensor using CsI deposition in the ionization chamber

  • Souri, R.;Negarestani, A.;Souri, S.;Farzan, M.;Mahani, M.
    • Nuclear Engineering and Technology
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    • v.50 no.5
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    • pp.751-757
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    • 2018
  • In this article, a UV sensor that is an appropriate tool for fire detection has been designed and constructed. The structure of this UV sensor is an air-filled single-wire detector that is able to operate under normal air condition. A reflective CsI photocathode is installed at the end of the sensor chamber to generate photoelectrons in the ion chamber. An electric current is produced by accelerating photoelectrons to the anode in the electric field. The detector is able to measure the intensity of the incident UV rays whenever the current is sufficiently high. Therefore, the sensitivity coefficient of this sensor is found to be $7.67{\times}10^{-6}V/photons/sec$.

Determination of isovitexin from Lespedeza cuneata using a validated HPLC-UV method

  • Lee, Ju Sung;Paje, Leo Adrianne;Yoo, Sang-Woo;Lee, Seong;Ku, Ja-Jung;Lee, Sanghyun
    • Journal of Applied Biological Chemistry
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    • v.64 no.1
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    • pp.63-67
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    • 2021
  • Isovitexin, a marker compound with various pharmacological activities, in Lespedeza cuneata, was analyzed using high performance liquid chromatography coupled with UV (HPLC/UV). There are no previous reports on using L. cuneata as the source material for the quantification of isovitexin. In this study, we developed an optimized method using HPLC-UV analysis, which was validated using various parameters. Our method demonstrated high specificity, and good separation of the chromatographic peak was achieved. Parameters such as linearity (r2≥0.9997), precision, and accuracy indicated that our proposed analytical method had good reliability and sensitivity. These results demonstrate the utility and convenience of our method for rapidly quantifying isovitexin in L. cuneata extracts.

The Variation of UV Radiation by Cloud Scattering at King Sejong Station in West Antarctica (남극 세종기지에서의 구름 산란에 의한 자외선 변화)

  • Lee, Kyu-Tae;Lee, Bang-Yong;Won, Young-In;Kim, Youn-Joung;Lee, Won-Hak;Jee, Joon-Bum
    • Ocean and Polar Research
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    • v.26 no.2
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    • pp.133-143
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    • 2004
  • For the purpose of understanding the cloud scattering effect of UV radiation at King Sejong station In West Antarctica, we analyzed the data measured by UV-Biometer at surface and compared its result with solar radiation model. The parameterization of UV radiation by cloud ice crystal was applied to solar radiation model and the sensitivity of this model for the variation of ice crystal was tested. The cloud optical thickness was calculated by using this solar radiation model. It was compared the result from calculation with CERES satellite data. In solar radiation model, the UV radiation was less scattered with increase of ice crystal size in cloud and this scattering effect was more important to UV-A radiation than Erythemal UV-B radiation. But scattering effects by altitude of cloud was not serious. The calculated cloud optical thicknesses in Erythemal UV-B and UV-A region were compared with CERES satellite data and the result by UV-A was more accurate than Erythemal UV-B region.

Differential Responses of Three Cyanobacteria to UV-B and Cd

  • Atri, Neelam;Rai, Lal-Chand
    • Journal of Microbiology and Biotechnology
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    • v.13 no.4
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    • pp.544-551
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    • 2003
  • Interactive effects of UV-B and Cd on growth, pigment, photosynthesis, and lipid peroxidation have been studied in Anabaena, Microcystis, and Nostoc; all the tested cyanobacteria showed differential sensitivity to different dosage of UV-B and Cd alone as well as in combination. Phycocyanin was severely affected by UV-B and Cd by all the strains; the degree of pigment bleaching was most pronounced in Anabaena followed by Microcystis and Nostoc. $UV-B_2+Cd_2$ produced nearly 83, 78, and 65% inhibition of phycocyanin in Anabaena, Microcystis, and Nostoc, respectively. The above treatment also significantly decreased the contents of Chl ${\alpha}$ and carotenoid. Presence of capsule in Microcystis protected the phycocyanin bleaching as compared to decapsulated cells. Laboratory-grown Microcystis revealed about 75 and 80% inhibition, following $UV-B_2+Cd_2$ treatment, respectively. in capsulated and decapsulated cells. Damage caused by Cd was more pronounced than UV-B. Inhibition of photosynthesis did occur in all the test strains, being maximum in Anabaena. PS II was the most sensitive component of the electron transport chain, showing 84, 80, and 70% inhibition in Anabaena, Microcystis, and Nostoc, respectively. As compared to control, significant lipid peroxidation (6.5-fold higher) was observed in Anabaena with $UV-B_2+Cd_2$, $^{14}C-uptake$ was more susceptible to Cd and Uv-B than oxygen-evolution. Approximately 84, 80, and 76% inhibition of $^{14}C-uptake$ was observed in Anabaena, Microcystis, and Nostoc, respectively. Similarly, $UV-B_2+Cd_2$ inhibited APT content of Anabaena by 87%. This ,study suggests that inhibition of carbon fixation was due to decreased ATP content of the test cyanobacteria by UV-B+Cd, where Anabaena was the most sensitive and Nostoc the most tolerant.

Determination of Sesamin and Sesamolin in Sesame (Sesamum indicum L.) Seeds Using UV Spectrophotometer and HPLC

  • Kim, Kwan-Su;Lee, Jung-Ro;Lee, Joon-Seol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.1
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    • pp.95-100
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    • 2006
  • Sesamin and sesamolin, antioxidant lipidsoluble lignan compounds, are abundant in sesame (Sesamum indicum L.) seed oil and provide oxidative stability of oil related to sesame quality. The sesamin and sesamolin contents of 403 sesame land races of Korea were determined by HPLC analysis of methanol extract (HPLC value), and their total lignan content was compared with those by using UV-Vis spectrophotometric analysis (UV method) of methanol (UV-MeOH value) and hexane (UV-Hexane value) extracts. HPLC values of total lignan content were strongly associated with UV-Hexane (r=0.705**) and UV-MeOH (r=0.811**) values. The UV values from both the extracts were 3.8-4.7 times higher than those of HPLC values. Lignan content was overestimated by UV method because total compounds in the mixture solution were quantified by absorbing at the same ultraviolet wavelength as in HPLC method. UV method could more rapidly analyze small amount of sample with higher sensitivity of detection than HPLC method. Average contents of lignans in sesame germplasm evaluated in this study were $2.09{\pm}1.02mg/g$ of sesamin, and $1.65{\pm}0.61mg/g$ of sesamolin, respectively, showing significant variation for lignan components. The results showed that UV method for the determination of sesamin and sesamolin could be practically used as a faster and easier method than HPLC by using the regression equations developed in this study.

Cholesteric Liquid Crystals as Multi-Purpose Sensor Materials

  • Lisetski, L.N.
    • Journal of Radiation Protection and Research
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    • v.30 no.1
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    • pp.27-30
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    • 2005
  • New possibilities are discussed for cholesteric liquid crystals (CLC) as sensor materials for detection of ionizing radiation, biologically active UV radiation, and the presence of hazardous vapors in atmosphere. A distinguishing property of CLC-based detectors is their 'bioequivalence', i.e., mechanisms of their response to external factors essentially imitate the corresponding mechanisms of biological tissues. Such detectors can ensure sufficiently high sensitivity to make feasible their use as alarm indicators or in biophysical studies. Specific examples ate given of sensor compositions and their response characteristics.