Browse > Article
http://dx.doi.org/10.3839/jabc.2021.010

Determination of isovitexin from Lespedeza cuneata using a validated HPLC-UV method  

Lee, Ju Sung (Department of Plant Science and Technology, Chung-Ang University)
Paje, Leo Adrianne (Department of Plant Science and Technology, Chung-Ang University)
Yoo, Sang-Woo (Research & Development Center, Natural Way Co., Ltd.)
Lee, Seong (Dankook University Hospital Research Institute of Clinical Medicine)
Ku, Ja-Jung (Forest Bioinformation Division, National Institute of Forest Science)
Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.1, 2021 , pp. 63-67 More about this Journal
Abstract
Isovitexin, a marker compound with various pharmacological activities, in Lespedeza cuneata, was analyzed using high performance liquid chromatography coupled with UV (HPLC/UV). There are no previous reports on using L. cuneata as the source material for the quantification of isovitexin. In this study, we developed an optimized method using HPLC-UV analysis, which was validated using various parameters. Our method demonstrated high specificity, and good separation of the chromatographic peak was achieved. Parameters such as linearity (r2≥0.9997), precision, and accuracy indicated that our proposed analytical method had good reliability and sensitivity. These results demonstrate the utility and convenience of our method for rapidly quantifying isovitexin in L. cuneata extracts.
Keywords
High-performance liquid chromatography; Isovitexin; Lespedeza cuneata; Method validation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zucolotto SM, Fagundes C, Reginatto FH, Ramos FA, Castellanos L, Duque C, Schenkel EP (2012) Analysis of C-glycosyl flavonoids from South American Passiflora species by HPLC-DAD and HPLC-MS. Phytochem Anal 23: 232-239   DOI
2 Close CA, Pereira JH, Yariwake M (2005) Distinction of the C-glycosylflavone isomer pairs orientin/isoorientin and vitexin/isovitexin using HPLC-MS exact mass measurement and in-source CID. Phytochem Anal 16: 295-301   DOI
3 Wang J, Tang F, Yue Y, Guo X, Yao X (2010) Development and validation of an HPTLC method for simultaneous quantitation of isoorientin, isovitexin, orientin, and vitexin in bamboo-leaf flavonoids. J AOAC Int 93: 1376-1383   DOI
4 Moheb A, Ibrahim RK, Roy R, Sarhan F (2011) Changes in wheat leaf phenolome in response to cold acclimation. Phytochemistry 72: 2294-2307   DOI
5 Wang C, Zhou B, Palm HL (2008) Detecting invasive Sericea Lespedeza (Lespedeza cuneata) in Mid-Missouri Pastureland using hyperspectral imagery. Environ Manag 41: 853-862   DOI
6 Kwon DJ, Kim JK, Ham YH, Bae YS (2007) Flavone glycosides from the aerial parts of Lespedeza cuneata. J Korean Soc Appl Biol Chem 50: 344-347
7 Baek J, Lee D, Lee TK, Song JH, Lee JS, Lee S, Yoo SW, Ki SK, Moon E, Lee S, Kim KH (2018) (−)-9'-O-(α-L-Rhamnopyranosyl)lyoniresinol from Lespedeza cuneata suppresses ovarian cancer cell proliferation through induction of apoptosis. Bioorg Med Chem Lett 28: 122-128   DOI
8 Baek J, Lee TK, Song JH, Choi E, Ko HJ, Lee S, Choi SU, Lee S, Yoo SW, Kim SH, Kim KH (2018) Lignan glycosides and flavonoid glycosides from the aerial portion of Lespedeza cuneata and their biological evaluations. Molecules 23: 1920   DOI
9 Sean KM (2012) Natural products research. Nat Prod Chem Res 1: e101
10 Yoo G, Park SJ, Lee TH, Yang H, Baek YS, Kim N, Kim YJ, Kim SH (2015) Flavonoids isolated from Lespedeza cuneata G. Don and their inhibitory effects on nitric oxide production in lipopolysaccharidestimulated BV-2 microglia cells. Pharmacogn Mag 11: 651-656   DOI
11 Woldeyes S, Adane L, Tariku Y, Muleta D, Begashaw T (2012) Evaluation of antibacterial activities of compounds isolated from Sida rhombifolia Linn. (Malvaceae). Nat Prod Chem Res 1: 101
12 Stalikas CD (2007) Extraction, separation, and detection methods for phenolic acids and flavonoids. J Sep Sci 30: 3268-3295   DOI
13 Koes RE, Quattrocchio F (1994) The flavonoid biosynthetic pathway in plants: Function and evolution. BioEssays 16: 123-132   DOI
14 Benavente-Garcia O, Castillo J, Marin FR, Ortuno A, Del Rio JA (1997) Uses and properties of citrus flavonoids. J Agric Food Chem 45: 4505-4515   DOI
15 Chuarienthong P, Lourith N, Leelapornpisid P (2010) Clinical efficacy comparison of anti-wrinkle cosmetics containing herbal flavonoids. Int J Cosm Sci 32: 99-106   DOI
16 Malinowska P (2013) Effect of flavonoids content on antioxidant activity of commercial cosmetic plant extracts. Herba Polonica 59: 63-75   DOI
17 Zhang J, Yuan K, Zhou WL, Zhou J, Yang P (2011) Studies on the active components and antioxidant activities of the extracts of Mimosa pudica Linn. from southern China. Pharmacogn Mag 7: 35-39   DOI
18 Choi JS, Islam MN, Ali MY, Kim EJ, Kim YM, Jung HA (2014) Effects of C-glycosylation on anti-diabetic, anti-Alzheimer's disease and anti-inflammatory potential of apigenin. Food Chem Toxicol 64: 27-33   DOI
19 Huang ST, Chen CT, Chieng KT, Huang SH, Chiang BH, Wang LF, Kuo HS, Lin CM (2005) Inhibitory effects of a rice hull constituent on tumor necrosis factor alpha, prostaglandin E2, and cyclooxygenase-2 production in lipopolysaccharide-activated mouse macrophages. Ann N Y Acad Sci 1042: 387-395   DOI
20 Lin CM, Huang ST, Liang YC, Lin MS, Shih CM, Chang YC, Chen TY, Chen CT (2005) Isovitexin suppresses lipopolysaccharide-mediated inducible nitric oxide synthase through inhibition of NF-kappa B in mouse macrophages. Planta Med 71: 748-753   DOI
21 Lee J, Lee AY, Quilantang N, Geraldino PJ, Cho E, Lee S (2019) Antioxidant activity of avicularin and isovitexin from Lespedeza cuneata. J Appl Biol Chem 62: 143-147   DOI
22 de Oliveira DR, Zamberlam CR, Gaiardo RB, Rego GM, Cerutti JM, Cavalheiro AJ, Cerutti SM (2014) Flavones from Erythrina falcata are modulators of fear memory BMC Complement. Altern Med 14: 288-301
23 Peng XF, Zheng ZP, Cheng KW, Shan F, Ren GX, Chen F, Wang MF (2008) Inhibitory effect of mung bean extract and its constituents vitexin and isovitexin on the formation of advanced glycation end products. Food Chem 106: 475-481   DOI
24 Landim LP, Feitoza GS, Costa JGM (2012) Development and validation of a HPLC method for the quantification of three flavonoids in a crude extract of Dimorphandra gardneriana. Braz J Pharmacogn 23: 58-64   DOI
25 Fu YJ, Zu YG, Liu W, Zhang L, Tong MH, Efferth T, Kong Y, Hou CL, Chen LY (2008) Determination of vitexin and isovitexin in pigeonpea using ultrasonic extraction followed by LC-MS. J Sep Sci 31: 268-275   DOI
26 Jang D, Jung YS, Kim MS, Oh SE, Nam TG, Kim DO (2019) Developing and validating a method for separating flavonoid isomers in common buckwheat sprouts using HPLC-PDA. Foods 8: 549   DOI
27 Fu Y, Zu Y, Liu W, Hou C, Chen L, Li S, Shi X, Tong M (2007) Preparative separation of vitexin and isovitexin from pigeonpea extracts with macroporous resins. J Chromatogr A 1139: 206-213   DOI