• Journal of Photoscience
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• 제9권2호
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• pp.400-402
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• 2002

We collected seeds of Solidago virgaurea plants growing at different altitudes on the Mt Hakusan area in Japan and cultivated them in a naturally-lit green house. Three-week-old seedlings were irradiated with supplemental UV-B for 12 h each day for 1 and 2 weeks. After a week of itradiation the seedlings of the population collected from the higher altitude at Oh-nanjiho (ON) had accumulated more anthocyanins than those from the lower altitude at Bettoh-deai (BD). Levels of anthocyanins in the ON seedlings were highly correlated with the dose of UV-B radiation and the correlation was also observed after 2 weeks. The growth of the third leaves was retarded by UV-B radiation in both populations. The extent of growth retardation in the third leaves was correlated with the dose of UV -B radiation in both populations. However, no significant difference in the extent of leaf area growth was observed between the ON and BD populations. The increase in plant fresh weight was extensively inhibited in the ON seedlings after 1 week of UV-B radiation. The inhibition was recovered to those in the BD population by 2 weeks irradiation. These results indicate that these populations respond differentially to supplementary UV -B radiation during the first week. Because flavonoids such as anthocyanins play an important role in protection against UV-B radiation in many plants, populations growing at higher altitude may be better able to adapt to increased global levels of UV-B radiation.

• Molecular & Cellular Toxicology
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• 제3권1호
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• pp.23-30
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• 2007

The effects of high energy short wave solar radiation on human skin have received much publicity as the major cause of accelerated skin ageing and of skin cancers. To meet public demand, the cosmetic industry has developed sun protection factor products, which contain a variety of so-called "UV filters", among others benzophenone (BP) and its metabolites are the widely used UV filters. UV filters are also used to prevent UV light from damaging scents and colors in a variety of cosmetics products and to protect of plastic products against light-induced degradation. There are many variants of BP in use. In this respect, to regulate and to evaluate the hazardous effect of BP-type UV filters will be important to environment and human health. The genotoxicity of 7 BP-type UV filters was evaluated in L5178Y $(tk^{+/-})$ mouse lymphoma cells in vitro. BP, benzhydrol, 4-hydroxybenzophenone 2-hydroxy-4-methoxybenzophenone and 2, 4-dihydroxybenzophenone did not induce significant mutation frequencies both in the presence and absence of metabolic activation system. 2, 2'-Dihydroxy-4-methoxybenzophenone appeared the positive results at the highest dose, i.e. 120.4 ${\mu}g/mL$ only in the absence of metabolic activation system. And also, 2, 3, 4-trihydroxybenzophenone revealed a significant increase of mutation frequencies in the range of 138.1-207.2 ${\mu}g/mL$ in the absence of metabolic activation system and 118.3-354.8 ${\mu}g/mL$ in the presence of metabolic activation system. Through the results of MLA with 7 BP-type UV filters in L5178Y cells in vitro, we may provide the important clues on the genotoxic potentials of these BP-type UV filters.

• 한국의류학회지
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• 제33권2호
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• pp.256-265
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• 2009

The purpose of this study is to check color change, color fastness, increase wt., antibiosis, and UV-protection efficiency depending on gallnut concentrations and mordanting methods, when silk fabrics dye with gromwell according to pH. This results will contribute in developing of natural mordant with multi function. The results are as follows. ${\lambda}_{max}$ of Gallnut extracts was near 299 nm. When gallnut was used as a mordant, at all pH levels, pre-mordanted fabrics had red color and post-mordanted ones had red-purple color which was closed to natural color of gromwell. Brightness of post-mordanted fabrics was higher than that of pre-mordanted fabrics. In the case of chroma, pre-mordanted fabrics was higher than post-mordanted fabrics. There was no significant difference of color, brightness, and chroma depending on gallnut concentration. As mordanting concentration increased, fabric weight gradually went up and increase weight reached maximum $17{\sim}19%$. At all pH levels, color fastness improved by pre-mordanting and post-mordanting, and it showed the maximum $4{\sim}5$ grade of wet fastness and 5 grade of dry cleaning. Antibiosis of silk fabric was improved by gromwell dyeing and synthetic tannin mordanting. Antibiosis of gallnut extracts was excellent. The color fastness and antibiosis were preserved after 10 cycle dry cleaning. UV-protection efficiency was excellent by dyeing with gromwell and mordanting with gallnut.

• Journal of Ginseng Research
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• 제45권4호
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• pp.498-509
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• 2021

Background: A wide range of environmental factors, such as diseases, nutritional deficiencies, ageing, hormonal imbalances, stress, and ultraviolet (UV) radiation, may affect the structure and function of the skin that covers the entire surface of the human body. In this study, we investigated roles of red ginseng oil (RGO) in enhancing skin functions, including hair growth and skin protection, using mouse models. Methods: For hair growth experiment, shaved dorsal skins of C57BL/6 mice were topically applied with vehicle, RGO, RGO's major compounds, or minoxidil for consecutive 21 days and skin tissues were examined the hair growth promoting capacity. For skin protection experiment, SKH-1 hairless mice were topically applied with vehicle or RGO twice a day for three days prior to exposure to UVC radiation at 20 kJ/cm². Skin tissues were collected to evaluate skin protective effects of RGO. Results: Topical application of RGO to C57BL/6 mice effectively promoted hair regeneration by inducing early telogen-to-anagen transition and significantly increasing the density and bulb diameter of hair follicles. Major compounds, including linoleic acids and β-sitosterol, contributed to RGO-promoted hair growth. Treatment with RGO as well as its major components upregulated expression of hair growth-related proteins. Furthermore, in SKH-1 hairless mice, RGO had a protective effect against UVC-induced skin damage by inhibiting inflammation and apoptosis, as well as inducing cytoprotective systems. Conclusion: These data suggest that RGO may be a potent agent for improving skin health and thereby preventing and/or treating hair loss and protecting skin against UV radiation.

• 한국축산식품학회지
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• 제35권1호
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• pp.58-70
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• 2015

This study was conducted to examine the effect of egg shell membrane hydrolysates (ESMH) on wrinkle, UV, and moisture protection for cosmetic use. ESMH were fragmented as whole ESMH (before fractioning), Fraction I (> 10 kDa), Fraction II (3-10 kDa), and Fraction III (< 3 kDa). In order to test whether fractionated ESMH can be used for functional cosmetic materials, we examined not only the level of hyaluronic acid and collagen production, but also the MMP-1 activity using a HaCaT and CCD-986Sk cell line. Our study treated each sample of fractionated ESMH with different concentrations (0.01, 0.1, 1 mg/mL). In our in vivo research, we used hairless mice that had been exposed to UV-B to induce wrinkles for 7 wk, then applied Fraction I to the treatment group for 5 wk and then tested skin thickness, minimum erythema dose and moisture content. In addition, Fraction I was high in collagen and HA biosynthesis and it was better than TGF-${\beta}$ in improving of the skin. When TNF-${\alpha}$ caused MMP-1 activity in the CCD-986Sk cells, the whole ESMH and Fraction I proved to be effective in hindering the induction of collagenase depending on the concentration, and also showed outstanding effects in the suppression of skin aging. We found that the treatment group mice's UV-B radiation-induced skin damage was largely mitigated compared to that of the non-treatment group mice. Thus, we have concluded that EMSH helps to mitigate UV-B radiation-induced wrinkles, collagen, HA, MMP-1 activity and can be used for functional cosmetic materials.

• 한국의류학회지
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• 제44권5호
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• pp.906-922
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• 2020

Efficacy of Flos Sophorae as UV protective dye was examined in comparison to standard dyes of quercetin and rutin. Cotton was dyed using Flos Sophorae extract, quercetin, and rutin with 2 different concentrations for each dye. Each type of dyeing applied a temperature of 70℃ or 90℃ and a time of 30 min or 60 min. Color values of dyed samples were examined using a spectrocolorimeter. HPLC-DAD analysis indicated the amount of quercetin and rutin contained in Flos Sophorae used in the study. UPF values of dyed samples were measured using an ultraviolet transmittance analyzer. Experimental results indicated that dyeing cotton with Flos Sophorae extract, quercetin, or rutin increased the UPF value and decreased the UVA and UVB transmittance (%) regardless of dyeing conditions. A higher rank of UPF values were shown in cotton dyed using the Flos Sophorae extract that implied the possible concerted effect of quercetin, rutin, and flavonoid compounds contained in Flos Sophorae. The results indicated that Flos Sophorae is an effective natural dye that can improve the UV protective function of summer cotton fabric.

• 대한화장품학회지
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• 제26권1호
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• pp.41-58
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• 2000

최근 환경오염으로 인한 지구오존층의 파괴로 자외선의 지표면의 도달 량이 증가하고 그로 인한 인간에 대한 피해가 증가하고 있다 . 과도한 자외선의 피부에 대한 직접적인 노출은 피부에 많은 문제점을 야기하므로 일차적으로 자외선차단제를 이용하여 인체에 대한 직접적인 피해를 최소화 해야한다 . 현재 자외선 차단제는 유기자외선흡수제와 무기자외선산란제가 많이 사용이 되는데 적은량으로도 효과가 높은 유기자외선차단제는 사용 시 주의를 요하므로 국가별로 사용량과 사용여부에 대하여 규제를 하고 있다. 본 연구에서는 플라보노이드류 ,알카로이드류 같은 자외선 흡수 성분을 함유한 식물중 UV/vis spectrophotometer 에서 자외선 흡수 peak을 나타내는 금은화 , 포공영, 녹차 , 황금추출물을 이용하여 화장품의 자외선 흡수제로서의 사용가능성여부를 조사하였다. 또한 식물추출물이 가진 자극성 성분과 오염성, 그들이 가진 유효성분들을 보호하고자 실리콘을 이용한 겔화를 시도하였다 . 자연친화적인 천연물로써 식물추출성분을 이용 유기자외선흡수제의 사용량을 줄이는 자외선홉수보조제로서 가능성을 보여주었다.

• 한방안이비인후피부과학회지
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• 제20권2호통권33호
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• pp.47-67
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• 2007

Objective : As a result of increasing amount of ultraviolet ray, skin problems including sunburn, rapid skin aging, melanoma, and even skin cancer continue to rise. In the present study, the effect of oriental herbal extract, Daehwanggo(大黃膏,DH) and Daehwanggogasnagbakpi(大黃膏加桑白皮,DS), as external application, on the skin damage, was investigated. Methods : 30 mice were equally distributed into 3 groups : control, UVB-control and UVB-irradiated and DS-treated group. Also mouse melanoma cell lines were cultured. Tyrosinase inhibition was measured to analyze the UN-protection effect. Melanogenesis in the UV-irradiated melanoma cell lines was compared in DS-treated cell line and control cell line. Sample skin from the ear tissue of the 3 groups were analyzed to observe the inflammatory response, T cell differentiation, apoptosis of keratinocytes. Results : The tyrosinase was more significantly inhibited in the DS group compared to DH group. Antioxidative effects was more prominent in DS group when superoxide dismutase was measured. Both the DS- and DH-treated cell lines showed significantly reduced melanogenesis. The reduction of external skin damage including erythematous papule, eczema, keratinocyte, pyopoiesis was observed in the DS- and DH-treated sample cells. In terms of the effect on the skin damage, sunburn cell, activated skin mast cells, secretion of IL-12, manifestation of HSP70, hyperplasia of epithelial cells, MMP-9 and destruction of the collagen were all significantly improved in the DS-treated sample cells. Melanin cells and the apoptosis in the melanoma cell line were decreased. Conclusion : DH and DS were traditionally applied externally for the scald in the oriental medicine. The present study elucidated the possibility of herbal extracts to be used as ultraviolet protectives. Further investigations are needed to assure the clinical application.

• 한국수산과학회지
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• 제41권1호
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• pp.7-12
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• 2008

To develop a whitening agent, cytotoxicity of the soluble collagen isolated from Todarodes pacificus (CIT) was evaluated. CIT tested for cytotoxicity on human dermal fibroblast (CCD-986sk) was exhibited very low cytotoxicity. Because tyrosinase is the key enzyme for melanin biosynthesis, the use of various tyrosinase inhibitors is a common practice for whitening purpose in cosmetics. Tyrosinase inhibitory activity and melanin production assay were measured to confirm the whitening effect. The inhibitory effect of MMP-1 in UV-irradiated human dermal fibroblast was also performed. CIT showed strong inhibition potency on tyrosinase by 51.5% at 0.2 mg/mL which increased the inhibition by increasing the concentration of CIT, and showed 69.1% inhibition at 1.0 mg/mL. CIT showed strong inhibition effect on melanin production with 82% at 1.0 mg/mL. The CIT also reduced about 76% expression of MMP-1 in UV-irradiated CCD-986sk cell at 1.0 mg/mL. From the preliminary observations, we suggest that the collagen isolated from CIT could be a potential source of natural skin-whitening and anti-aging agents for the photo-damaged skin.

• 한국응용과학기술학회지
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• 제22권3호
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• pp.281-288
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• 2005

The silica nanoparticles were used as support of catalyst, filling material, electronic assembler, thin film material, and sensor material. And, the titania nanoparticles were used as pigment, dielectric substance, sensor and photocatalyst. In this paper, the spherical composite particles of $TiO_2/SiO_2$with narrow size distribution and phase pure were synthesized by ultrasonic spray pyrolysis method from $TiOSO_4$ and colloidal silica solution. Using ultrasonic apparatus, this starting solution was vaporized to droplets, and these droplets were induced into tube furnace by carrier gas. The resulting composite powder was characterized by scanning electron microscopy, X-ray diffraction analysis, TG-DTA, in vitro sun protection factor(SPF) and BET surface area analysis.