• 한국가금학회:학술대회논문집
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• 한국가금학회 1999년도 제16차 정기총회및학술발표회
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• pp.34-50
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• 1999

A cDNA encoding chicken interferon-gamma (chIFN-${\gamma}$) was amplified from P34, a CD4$^{+}$ T-cell hybridoma by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into pUC18. THe sequences of cloned PCR products were determined to confirm the correct cloning. Using this cDNA as probe, chicken genomic library from White Leghorn spleen was screened. Phage clones harboring chicken interferon-gamma (chIFN-${\gamma}$) were isolated and their genomic structure elucidated. The chIFN-${\gamma}$ contains 4 exons and 3 introns spanning over 14 kb, and follows the GT/AG rule for correct splicing at the exon/intron boundaries. The four exons encode 41, 26, 57 and 40 amino acids, respectively, suggesting that the overall structure of IFN-${\gamma}$ is evolutionairly conserved in mammalian and avian species. The 5’-untranslated region and signal sequences are located in exon 1. Several AT-rich sequences located in the fourth exon may indicate a role in mRNA turnover. The 5’-flanking region contains sequences homologous to the potential binding sites for the mammalian transcription factors, activator protein-1(AP-1) activator protein-2(AP-2) cAMP-response element binding protein(CREB), activating transcription factor(ATF), GATA-binding fator(GATA), upstream stimulating factor(USF), This suggests that the mechanisms underlying transcriptional regulation of chicken and mammalian IFN-${\gamma}$ genes may be similar.r.

• 한국정보시스템학회지:정보시스템연구
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• 제22권1호
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• pp.201-223
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• 2013

모바일뱅킹은 빠르게 성장하고있으며 은행서비스의 많은 부분이 모바일뱅킹에서 제공되고 있다. 하지만, 모바일뱅킹을 사용자들이 수용하는데 개인의 혁신성이 어떠한 영향을 미치는지에 대한 연구는 많이 이루어지지 않고 있다. 불행히도 모바일뱅킹은 그 특성상 제한된 화면을 사용자들이 사용하기가 용이하지 않다. 본 연구는 개인혁신성(PI)의 관점에서 모바일뱅킹을 수용하는데 영향을 미치는 요인을 밝히고 자하였다. 특히 다른 정보시스템에서 많이 사용된 지각된 유용성(USF) 및 사용의 용이성(EOU) 변수뿐만 아니라 지각된 즐거움(ENJ)의 요인을 추가하여 모바일뱅킹 수용에서 영향요인을 분석하고자 하였다. 또한, 본 연구에서는 336명의 모바일뱅킹의 사용자들에게 설문을 실시하였으며, 응답자들을 두 그룹(개인 혁신성이 높은 그룹과 낮은 그룹)으로 분류하여 구조방정식모형에서 집단간차이를 분석하였다. 본 연구결과 개인 혁신성(PI) 모바일뱅킹 수용에 영향을 미치는 것으로 분석되었다. 세부적으로 분석해 보면 개인혁신성이 높은 그룹이 낮은 그룹 보다 더 많은 영향을 받는 것으로 분석되었다. 특히, 사용의도와 사용의 유용성 그리고 지각된 즐거움 사이의 경로계수 값의 크기가 개인혁신성이 높은 그룹이 낮은 그룹보다 높았다. 여기서 지각된 즐거움의 경우 개인 혁신성이 높은 그룹이 낮은 그룹 보다 더 많은 영향을 받는 것으로 분석되었다. 이러한 연구의 결과는 모바일뱅킹 서비스의 성장과 확대에 실무적으로 사용할 수 있을 것으로 분석된다.

• Fisheries and Aquatic Sciences
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• 제12권2호
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• pp.90-97
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• 2009

The cytoskeletal $\beta$-actin gene and its 5'-upstream region were isolated and characterized in the rockbream (Oplegnathus fasciatus). Complementary DNA of the rockbream $\beta$-actin represented a 1,125 bp of an open reading frame encoding 375 amino acids, and the rockbream $\beta$-actin cDNA and deduced amino acid sequences were highly homologous to those of other vertebrate orthologs. At the genomic level, the $\beta$-actin gene also exhibited an organization typical of vertebrate cytoskeletal actin genes (2,159 bp composed of five translated exons interrupted by four introns) with a conserved GT/AG exon-intron splicing rule. The putative non-translated exon predicted in the rockbream $\beta$-actin gene was much more homologous with those of teleostean $\beta$-actin genes than those of mammals. The 5'-upstream regulatory region isolated by genome walking displayed conserved and essential elements such as TATA, CArG and CAAT boxes in its proximal part, while several other immune- or stress-related motifs such as those for NF-kappa B, USF, HNF, AP-1 and C/EBP were in the distal part. Semi-quantitative RT-PCR assay results demonstrated that the rockbream $\beta$-actin transcripts were ubiquitously but different-tially expressed across the tissues of juveniles.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.359-365
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• 2013

The high mobility group box 1 (HMGB1) protein is a multifunctional cytokine-like molecule that plays an important role in the pathogenesis of tumors. In this study, real-time polymerase chain reactions and Western blot assays indicated that HMGB1 transcriptional activity and protein level are increased in $Tax^+$-T cells (TaxP). To clarify the mechanisms, a series of HMGB1 deletion reporter plasmids (pHLuc1 to pHLuc6) were transfected into $Tax^-$-T cells (TaxN, Jurkat) and $Tax^+$-T cells (TaxP). We found that promoter activity in $Tax^+$-T cells to be higher than that in $Tax^-$-T cells, indicating a significant increase in pHLuc6. Bay11-7082 (NF-${\kappa}B$ inhibitor) treatment did not block the enhancing effect. Chromatin immunoprecipitation assays revealed that Tax was retained on a HMGB1 promoter fragment encompassing -1163 to -975. Bioinformatics analysis showed six characteristic cis-elements for CdxA, AP-1, AML-1a, USF, v-Myb, and C/EBP in the fragment in question. Mutation of cis-elements for C/EBP reduced significant HMGB1 promoter activity induced by Tax. These findings indicate that Tax enhances the expression of HMGB1 gene at the transcriptional level, possibly by interacting with C/EBP.

• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5281-5286
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• 2013

Purpose: Prostate cancer caused by the abnormal disorderly growth of prostatic acinar cells is the most prevalent cancer of men in western countries. We aimed to screen out differentially expressed genes (DEGs) and explore small molecule drugs for prostate cancer. Materials and Methods: The GSE3824 gene expression profile of prostate cancer was downloaded from Gene Expression Omnibus database which including 21 normal samples and 18 prostate cancer cells. The DEGs were identified by Limma package in R language and gene ontology and pathway enrichment analyses were performed. In addition, potential regulatory microRNAs and the target sites of the transcription factors were screened out based on the molecular signature database. In addition, the DEGs were mapped to the connectivity map database to identify potential small molecule drugs. Results: A total of 6,588 genes were filtered as DEGs between normal and prostate cancer samples. Examples such as ITGB6, ITGB3, ITGAV and ITGA2 may induce prostate cancer through actions on the focal adhesion pathway. Furthermore, the transcription factor, SP1, and its target genes ARHGAP26 and USF1 were identified. The most significant microRNA, MIR-506, was screened and found to regulate genes including ITGB1 and ITGB3. Additionally, small molecules MS-275, 8-azaguanine and pyrvinium were discovered to have the potential to repair the disordered metabolic pathways, abd furthermore to remedy prostate cancer. Conclusions: The results of our analysis bear on the mechanism of prostate cancer and allow screening for small molecular drugs for this cancer. The findings have the potential for future use in the clinic for treatment of prostate cancer.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제26권2호
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• pp.7-17
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• 2020

Purpose: The purpose of this study is to identify the operational status and current management of Sub-Health Centers in Limpio, Paraguay. Second, understanding the use of Sub-Health Centers and Perceptions of Sub-Health Centers in Limpio, Paraguay. Third, Providing policy implications for strengthening the Health Delivery System in Paraguay. Methods: The survey of the current status of sub-health centers in Limpio was conducted with observation and interview. Utilization of Sub-Health centers was analyzed in the 2018 Paraguay Community Health Survey. A face to face interview was conducted to complete a questionnaire and 831 samples were collected for the study. Results: In order to perform the normal function of the sub-health center, it will be necessary to allocate manpower that meets the standard. A common problem with sub-health centers in Limpio is that they have an environment vulnerable to rain. Currently, there are no health promotion and communicable disease management programs in sub-health center. Satisfaction of users about treatment, equipment, medicines and cleanleness of rooms. Implications: First, it is necessary to allocate human resources and organize spaces according to the standard. Second, there was a problem caused by moisture, and continuous maintenance and repair are required. Third, water and sewage related facilities must be safely improved to prevent contamination of groundwater. Forth, it is necessary to implement a program that fits the role of the sub-health center. Fifth, it is necessary to form a health delivery system considering the accessibility of residents. Finally, it is necessary to discuss the location of sub-health center considering travel time of Limpio residents.

• Journal of Yeungnam Medical Science
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• 제26권1호
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• pp.24-29
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• 2009

결과적으로 영남대학교 의과대학에서는 3학년 학생을 대상으로 2008년도에 처음으로 해외임상실습을 시행하게 되어 2년간 새로운 경험을 하게 되었으며 외국의 병원까지도 교류의 기회를 넓히게 되었다. 향후에는 좀 더 영역을 넓히고 전 학생이 참가할 수 있는 프로그램을 개발하여 글로벌 의사양성이라는 교육목표를 완수하려는 지속적인 관심과 노력이 필요할 것으로 생각한다.