• Molecular & Cellular Toxicology
• /
• v.4 no.4
• /
• pp.338-347
• /
• 2008

Yeast RAD2, a yeast homolog of human XPG gene, is an essential element of nucleotide excision repair (NER), and its deletion confers UV sensitivity and NER deficiency. 6-Azauracil (6AU) sensitivity of certain rad2 mutants revealed that RAD2 has transcription elongation function. However, the fundamental mechanism by which the rad2 mutations confer 6AU sensitivity was not clearly elucidated yet. Using an insertional mutagenesis, PUF4 gene encoding a yeast pumilio protein was identified as a deletion suppressor of rad2${\Delta}$ 6AU sensitivity. Microarray analysis followed by confirmatory RT-qPCR disclosed that RAD2 and PUF4 regulated expression of HPT1 and URA3. Overexpression of HPT1 and URA3 rescued the 6AU sensitivity of rad2${\Delta}$ and puf4${\Delta}$ mutants. These results indicate that 6AU sensitivity of rad2 mutants is in part ascribed to impaired expression regulation of genes in the nucleotide metabolism. Based on the results, the possible connection between impaired transcription elongation function of RAD2/XPG and Cockayne syndrome via PUF4 is discussed.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• v.2
• /
• pp.143-148
• /
• 2006

The goal of this paper is to analyze, through simulations and experiments, GNSS interference mitigation performance under various types of antenna structures against wideband and narrowband interferences using spatial-temporal adaptive signal processing (STAP) techniques. The STAP approach, which combines spatial and temporal processing, is a viable means of GNSS array signal processing that enhancing the desired signal quality and providing protection against interference. In this paper, we consider four types of 3D antenna array structure - Uniform Linear Array (ULA), Uniform Rectangular Array (URA), Uniform Circular Array (UCA), and the Single-Ring Cylindrical Array (SRCA) under an interference environment. Analytical evaluation and simulations are performed to investigate the system performance. This is followed by simulation GPS orbits in interfered environment are used to evaluate the STAP performance. Furthermore, experiments using a 2x2 URA hardware simulator data show that with the removal of wideband and narrowband interference through the STAP techniques, the signal tracking performance can be enhanced.

• The Korean Journal of Mycology
• /
• v.17 no.4
• /
• pp.209-213
• /
• 1989

Transformation of an auxotrophic requirement for uracil in Pleurotus florida P101 has been achieved using chimeric vector containing Aspergillus nidulans ans 1, and Neurospora crassa pyr 4 DNA. Protoplasts of $Ura^-$strains of P. florida were incubated with plasmid pDJB3 containing the cloned pyr 4 gene in the presence of polyethylene glycol and $CaCl_2$. Transformants could grow on MMM showing mitotical stability. Southern hybridization analysis of DNA isolated from transformants showed that the Neurospora pyr 4 gene and vector sequence might be integrated into the P. florida chromosomes. As the transformants were monokaryon, each transformant was mated with the other monokaryon. Fruitbody shape of untransformant was eroded type but those of transformants were eroded type, funnel type, plane type and ungrowing cap type.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.42 no.4
• /
• pp.723-730
• /
• 2017

In this paper, we consider an online compensation algorithm considering the mutual coupling and suggest a new GPS antenna array to apply. To evaluate the anti-jamming performance for the proposed antenna array, ULA and URA, we divide direction finding of multiple jamming signals into environments. 1. there is no mutual coupling. 2. there is mutual coupling but no compensation. 3. mutual coupling is compensated. RMSE analysis showed that the online compensation algorithm works and that peak detection is possible for multiple jamming signals.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.3
• /
• pp.271-276
• /
• 2009

Functional gene studies in the cultivated white button mushroom Agaricus bisporus have been constrained by the absence of effective gene-silencing tools. Using two endogenous genes from A. bisporus, we have tested the utility of dsRNA hairpin constructs to mediate downregulation of specific genes. Hairpin constructs for genes encoding orotidine 5'-monophosphate decarboxylase (URA3) and carboxin resistance (CBX) were introduced into A. bisporus using Agrobacteriummediated transfection. Although predicted changes in phenotype were not observed in vitro, quantitative-PCR analyses indicated unambiguously that transcripts in several transformants were substantially reduced compared with the non-transformed controls. Interestingly, some hairpin transformants exhibited increased transcription of target genes. Our observations show that hairpin transgenic sequences can mediate downregulation of A. bisporus endogenous genes and that the technology has the potential to expedite functional genomics of the mushroom.

• Korean Journal of Microbiology
• /
• v.31 no.1
• /
• pp.48-53
• /
• 1993

For the purpose to improve the glucoamylase productivity of Saccharomyces diastaticus, we integrated STA 1 gene into chromosomal DNA of S. diastaticus using YIp vector. After construction of Ylp-STA by the subcloning of STAI (5.3 kb) into YIp5 vector, S. diastaticus GMT-II(a. ura3. STAJ) was transformed by Ylp-STA through homologous recombination at the chromosomal STAJ gene. So we obtained the tram formants that glucoamylase productivity was increased maximum six fold. These strains transformed by the multi-copy integration of Ylp-STA in chromosomal DNA were confirmed by Southern hybridization. And the integrated Ylp-STA was maintained stably during 30 mitotic divisions.

• Microbiology and Biotechnology Letters
• /
• v.23 no.5
• /
• pp.536-543
• /
• 1995

Multicopy integration vector is a very useful vector system in that they can be integrated into chromosomal DNA in several copies and stably maintained under non-selective conditions. To develop a multicopy integration vector system in the yeast Yarrowia lipolytica, P-type ribosomal DNA was cloned from Y lipolytica. A HindIII-BglII fragment of the cloned rDNA and a promoterless URA3 gene were inserted into pGEM1, generating multicopy integration vectors, pMIYL-1 and pMIYL-2. The rDNA fragment is for targeted homologous recombination between the vector and the chromosomal DNA of Y. lipolytica, and the promoterless URA3 gene is a defective selection marker for inducing multicopy integration. pMIYL-1 and pMIYL-2 have an unique restriction enzyme site, KpnI, and two unique restriction enzyme sites, KpnI and EcoRI, repectively, which can be used for targeting of the vectors into the rDNA of Y. lipolytica chromosomal DNA. After transformation of the vectors into Y. lipolytica, copy number and stability were analyzed by Southern hybridization. The vectors were found to be present in less than 5 copies per cell and were stably maintained during growth in non-selective media.

• Journal of Microbiology
• /
• v.40 no.3
• /
• pp.245-247
• /
• 2002

A genomic DNA library of the fungus Trametes versicolor was constructed in a yeast integration vector which contains the URA3 gene of the budding yeast Saccharomyces cerevisiae and the gene responsible for hygromycin B resistance, and fragments acting as autonomously replicating sequences (ARSes) in the budding yeast were identified from the genomic DNA library. Sixteen recombinant plasmids from the library transformed the budding yeast Saccharomyces cerevisiae to Ura+ at high frequencies. They were maintained stably under selective conditions, but were gradually lost from yeast cells at different rates under nonselective conditions, indicating that they contain eukaryotic origins of DNA replication and exist as extrachromosomal plasmids. Base sequences of four ARS DNAs among the 16 cloned fragments revealed that all or the four contain at least one 11 bp ［(A/T)TTTA(T/C)(A/G)TTT(A/T)］consensus sequence of the budding yeast ARS.

• Korean Journal of Microbiology
• /
• v.52 no.1
• /
• pp.116-119
• /
• 2016

Stable maintenance of telomere is required for cell proliferation and survival. Although telomerase is the primary means for telomere maintenance, recombination is another important pathway to maintain telomeres. In this study, we developed a genetic assay for telomere recombination using the internal $TG_{1-3}$ repeats present in subtelomeric regions of yeast. The recombination frequencies were dependent on the presence of the internal $TG_{1-3}$ repeats. PCR amplification of the regions near URA3 and CAN1 markers using genomic DNA isolated from $FOA^rCan^r$ colonies indicated that each isolate had lost the chromosome end including the markers. In addition, the recombination frequencies increased with longer internal $TG_{1-3}$ repeats. Our results suggest that the $FOA^rCan^r$ colony formation is the consequence of recombination between the internal and terminal $TG_{1-3}$ repeats.

• Animal Systematics, Evolution and Diversity
• /
• v.5 no.2
• /
• pp.89-105
• /
• 1989

농게(Ura arcuata) (달랑게과, 달랑게아과) 의 유생을 부화에서부터 첫 번째 게기까지 수온 $25^{\circ}C$ , 염분농도 33.3$\textperthousand$의 해수에서 사육하고, 각 유생기이 형태적인 특징을 기술 및 도식하였다. 이 종은 5기의 zoea와 1기의 megalopa 유생을 거쳐 첫 번째 게기로 변태하였다. 제 1 zoea 유생기는 제 1 소악의 내지에 4개의 말단강모와 제 2 악각의 기절과 내지에 각각 3개의 우상강모와 0, 0, 5 강모식을 가지고 촉각은 B형이었다. 이러한 특징들은 이미 보고된 같은 과의 유생들의 특징들과 잘 일치하고 있다.