• Journal of Life Science
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• v.14 no.1
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• pp.115-120
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• 2004

Lepista nuda is a world-wide species which has and international reputation as a excellent edible species. In this study, we investigated the genetic variation and taxonomic relationship of L. nuda and other five Tricholomataceae species were analyzed by random amplied polymorphic DNA (RAPD). 15 kinds of random primers were used. The distance matrix was calculated using UPGMA and phyolgenetic relationship were inferred by neighnor-joining (NJ) method. Various bands of 100bp∼1600bp were observed in electrophoretic patterns of RAPD. Nei's genetic distance was calculated using a total of 228 DNA bands identified, and phylogenetic tree was made. The Nei's genetic variations of L. nuda, Lepista surdida, Collybia peronata, Collybia confluens, Lyophyllum cinerascens, Laccara laccata were 0∼21.3%, 21.2∼28.0%, 15.4∼23.0%, 14∼21.8%, 16.5∼34.6%, and 12.4∼27.4%, respectively The consistency index, the retention index and homoplasy index were 0.5217, 0.5769 and 0.5156, respectively. Also, two groups could be made by NJ tree. The genetic distance between L. nuda and C. confluens was closer than that between L. nuda and L. sordida.

• Journal of Forest and Environmental Science
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• v.29 no.3
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• pp.200-210
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• 2013

Biochemical characteristics of 24 Pongamia pinnata genotypes (candidate plus trees) from three agroclimatic zones were estimated and molecular characterization through RAPD markers was done. Various biochemical characters viz. seed oil, total carbohydrates, protein, acid value and Iodine number recorded significant variation among different genotypes. The highest seed oil content was 41.87% while seeds of 14 genotypes recorded above average (32.11%) for the trait. Seed oil and protein content exhibited a significant positive correlation and moderate heritability. Out of the initially selected twenty-five random primers, twenty-two RAPD primers were found to be highly reproducible and produced a total of 183 loci of which 147 (80.32%) loci were polymorphic. Percentage of polymorphism varied from 44% to 100% with an average of 80.62%. High level of genetic variation was found among different genotypes of P. pinnata. Both molecular and oil content (biochemical) markers appeared useful in analyzing the extent of genetic diversity in Pongamia and the result of these analyses will help to better understand the genetic diversity and relationship among populations. Overall, the Pongamia genotypes included in the study showed a correlation with their geographical origins such that genotypes from the same region tend to have higher genetic similarity as compared to those from different regions. However, in UPGMA based Nei's analysis, some genotypes were found not to be grouped based on geographical origins possibly due to the exchange of germplasm over time between farmers across the regions. The results from oil content analyses showed that several genotypes in 'Central and Western Plateau' agroclimatic zone of Jharkhand displayed a good potential for high oil content. The study provides insight about P. pinnata populations in Jharkhand (India) and constitutes a set of useful background information that can be used as a basis for future breeding strategy and improvement of the species.

• Journal of Korean Society of Forest Science
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• v.103 no.2
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• pp.196-202
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• 2014

In order to select superior trees based on fruit characteristics and provide basic informations necessary for their improvement, total 107 individual trees of Sorbus alnifolia (Sieb. et Zucc.) K. Koch were selected from 11 wild populations in South Korea. After collecting normal fruit branch, we investigated morphological characteristics of fruit and then considered its relationship among the 11 populations by multivariate analysis method. Results from principal compound analysis showed that it represented 85.8% accumulated explanation from five principal compounds. According to cluster analysis based on fruit characteristics, the natural S. alnifolia populations were classified into four groups and Mt. Mani population was different from other populations. Selection effect with outstanding candidate trees including superior 5 individual trees (Gwangyo 1, Gwangyo 2, Deogyu 7, Mani 29, Mani 30) was estimated at 122.8%, 115.5% and 182.7% in fruit width, length and yield per fruit bunch, respectively. The object of this results will give us invaluable information about breeding by selection of S. alnifolia in south Korea.

• Journal of Sericultural and Entomological Science
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• v.45 no.1
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• pp.18-24
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• 2003

B. mandarina of Korean population apparently differs B. mori in isozyme analysis. Fourteen polymorphism occurred B. mandarina not in B. mori at 6 isozymes, Bph, Bes, Amy-hc, Ies, Ict-D, Ict-E. Korean population has shared with the Korean native strain of B. mori in B genotype of Bes, F of Amy-hc, n of Ict-E, M and S of Ict-H. These 5 genotype were known that detection only Korean native strains of B. mori. Nei's genetic distance based on the genotype of isozyme and hemolymph protein using 4 populations of B. mandarina varied from 0.0350 to 0.0624. The distances of 0.0350 is between Jinju and Chilgok population and between Jinju and Kosung population has the largest distances, 0.0624. In genus of Bombyx, B. mandarina and B. mori, genetic distance varied from 0.3822 to 0.5074. Phylogenetic tree obtained using the subprogram UPGMA of NTSYS represented that Bombyx devided two group, B. mandarina and B. mori. B. mandarina has genetic differences according to the population within the Korean peninsula, but that was not recognized genetic variation or divergence considering low values of genetic distance.

• Journal of Ecology and Environment
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• v.38 no.4
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• pp.647-654
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• 2015

A prostrate species of Codium (Bryopsidales, Chlorophyta) was collected from Daejin on the eastern coast of Korea. This alga is morphologically characterized by a prostrate, adherent or pulvinate, dark green thallus that is tightly attached to substratum. The utricles are strongly grouped and cylindrical to slightly clavate. Their apex is rounded to capitated, and it frequently has an alveolate ornament. Hair scars are found in the upper portion of the utricle. The gametangia grow on a short pedicel in the upper part of the utricle. In the phylogenetic tree based on molecular data, this alga is placed in the same clade as C. mozambiquense in UPGMA analysis, and nests in a sister clade of C. lucasii subsp. capense and C. mozambiquense in ML and NJ analyses. However, the genetic distance between the sequences of the Korean alga and the two species is 1.3-1.9%, while that between the Korean alga and C. lucasii from Japan is 1.1% within intraspecific range. The divergence value between the Korean alga and C. lucasii from the type locality (Australia) is 2.7% considered to be interspecific range. As based on this genetic divergence value, the Korean alga together with Japanese C. lucasii can be separated from genuine C. lucasii from the type locality. However, the Korean alga is identified as C. lucasii until those entities are morphologically characterized in species level. This is the first record of C. lucasii in Korea

• Journal of Forest and Environmental Science
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• v.24 no.1
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• pp.27-34
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• 2008

Level and distribution of genetic diversity in seven populations of Albizia lucida Benth. in eastern region of the Indian sub-continent were estimated using ISSR markers. Relatively higher level of genetic diversity within populations was observed in seven populations of A. lucida (mean of 0.38). From the result of AMOVA, majority of genetic diversity was allocated within populations (96.2%) resulting in a moderate degree of population differentiation. The observed distribution pattern of I-SSR variant among the populations was coincided with the typical pattern of long-lived woody tree species. Genetic relationships among the populations, reconstructed by UPGMA method, revealed two genetic groups. The population of Anugul and Bargarh turned out to be the most closely related despite a distance location between them. These formations will be of great value in the development of conservation plans for species exhibiting high levels of genetic differentiation due to fragmentation, such as indication of conservation unit size, which populations should be chosen as priority in conservation plans and which samples should be introduced in areas with a low number of individuals of A. lucida.

• Journal of Microbiology
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• v.33 no.2
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• pp.136-141
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• 1995

From a cluster of structural rRNA genes which has previsouly been cloned (Hwang and Kim, in submission; J. Microbiol. Biotechnol.), a 1.0-kb Eco RI fragment of DNA which shows significant homology to the 25S and rRNA s of Tricholoma matsutake was used for sequence analysis. Nucleotide sequence was bidirectionally determined using delection series of the DNA fragment. Comparing the resultant 1016-base sequence with sequences in the database, both the 3'end of 25S-rRNA gene and 5S rRNA gene were searched. The 5S rRNA gene is 118-bp in length and is located 158-bp downstream of 3'end of the 25S rRNA gene. IGSI and IGS2 (partial) sequences are also contained in the fragment. Multiple alignment of the 5S rRNA sequences was carried out with 5S rRNA sequences from some members of the subdivision Basidiomycotina obtained from the database. Polygenetic analysis with distance matrix established by Kimura's 2-parameter method and phylogenetic tree by UPGMA method proposed that T. matsutake is closely related to efibulobasidium allbescens. Secondary structure of 5S rRNA was also hypothesized to show similar topology with its generally accepted eukaryotic counterpart.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.1
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• pp.1-6
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• 2006

This experiment first cloned some microsatellite sequences for goose species by magnetic beads enriched method and studied the genetic structure research of 14 indigenous grey goose breeds using 19 developed and 12 searched microsatellite markers with middle polymorphism. According to the allele frequencies of 31 microsatellite sites, mean heterozygosity (H), polymorphism information content (PIC) and $D_A$ genetic distances were calculated for 31-microsatellite sites. The results showed that 25 of 31microsatellite sites were middle polymorphic, so the 25 microsatellite markers were effective markers for analysis of genetic relationship among goose breeds. The mean heterozygosity was between 0.4985 and 0.6916. The highest was in the Xupu (0.6916), and in the Yan was the lowest (0.4985) which was consistent with that of PIC. The phylogenetic tree was completed through analysis of UPGMA. Fencheng Grey, Shoutou, Yangjiang and Magang were grouped firstly, then Xongguo Grey, Wugang Tong, Changle and Youjiang were the second group; Gang, Yan Xupu and Yili were the third group; Yongkang Grey and Wuzeng were the fourth group. The results could provide basic molecular data for the research on the characteristics of local breeds in the eastern China, and a scientific basis for the conservation and utilization of those breeds.

• Development and Reproduction
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• v.23 no.4
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• pp.377-384
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• 2019

Genomic DNA extracted from representatives of two populations, Gunsan and Chinese, of Urechis spp. was amplified using PCR with several primers. The band-sharing (BS) value between individuals no. 05 from the Gunsan population and no. 22 from the Chinese population was 0.206, which was the lowest recognized value. Oligonucleotides primer OPC-04 revealed 44 unique loci, which distinguished the Chinese population. Primer OPB-17 allowed the discovery of 22 loci shared by the two populations, which were present in all samples. Based on the average BS results, individuals from the Gunsan population demonstrated lower BS values (0.661±0.012) than did those from the Chinese population (0.788±0.014; p<0.05). The shortest genetic distance (GD) displaying a noteworthy molecular difference was between individuals CHINESE no. 12 and no. 13 (GD=0.027). Individual no. 06 from the Gunsan population was most distantly related to CHINESE no. 22 (GD=0.703). A group tree of the two populations was constructed by UPGMA Euclidean GD analysis based on a total of 543 fragments generated using six primers. The explicit markers recognized in this study will be used for genetic analysis, as well as to evaluate the species security and proliferation of echiuran individuals in intertidal regions of the Korean Peninsula.

• The Korean Journal of Zoology
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• v.35 no.1
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• pp.80-86
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• 1992

The nucleotide sequences of 185 rRNAs of the five Korean decapods were partially determined by the direct sequencing method using the reverse transcriptase. ne average GC content of five species was 51.1% which is higher than that of yeast(45.0%) and lower than those of frog (53.0%) and rat (55.6%). This result follows the general patterns of the GC content in the nucleotides of the nucleic acid shown among the various phylogenetic groups. The average ratio of transrional/transversional nucleotide substitution of pairwise comparison among six species (including Anemia salina) was 1.200 $\pm$ 0.310 when whole region alas examined. However, the ratio showed some differences when the conservative regions and variable regions frere separatelv examined. The molecular phylogenies of the five species were constructed by using two different tree making methods. In general the results support the previously reported molecular phylogeny of the decapod crustaceans. However, our results indicate thats in the analysis of the sequence dat3, the UPGMA clustering method of the distance matrix method should be carefully employed after considering the rate of nucneotide substitution in the different regions of the molecule.