• 생명과학회지
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• 제9권2호
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• pp.176-181
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• 1999

본 연구는 누에(Bombyx mori)에서 cloning한 BomMAR의 염기서열을 기초로 하여 곤충 MLE(mariner-like ele-ment)의 계통분석을 통한 누에의 분자적 계통 관계를 이해하고자 수행하였다. 전체 10 종의 MLE중에서 15%의 낮은 상동성을 보이는 인간 MLE(Hsmarl)을 제외한 9종의 MLE의 DNA 염기서열을 이용한 UPGMA 분석 결과, BmoMAR을 포함한 10종의 MLE가 세 가지의 subfamily 로 grouping되는 것을 알 수 있었으며, 누에는 genetic distance 0.4332에서 같은 lepidoptera(나비목) 의 H. cecropia, almond moth, webworm 및 microcaddisfly와 함께 grouping 되었다. 따라서 이와 같은 결과는 MLE가 곤충의 계통분석에 유용한 molecular tool이 될 수 있음을 보여준다.

• 한국균학회지
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• 제28권1호
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• pp.32-37
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• 2000

본 연구는 국내 주요 송이 산지에서 생산되는 송이{Tricholoma matsutake(S. Ito et Imai) Sing}의 개체간, 그리고 지역간 유전적 다양성을 구명하기 위하여 실시하였다. 경상북도의 봉화, 울진, 고령, 청도의 4개 지역, 경상남도의 창녕, 하동, 함양의 3개 지역, 강원도의 양양, 인제의 2개 지역, 충청북도의 괴산, 전라북도의 남원을 포함하여 총 11개의 송이 산지를 대상으로 1994년부터 1997년까지 산지별로 $3{\sim}8$개의 균환에서, 그리고 각 균환마다 2개의 자실체를 채집하여 6개의 primer를 이용하여 Inter Simple Sequence Repeat Polymerase Chain Reaction(I-SSR PCR)법을 이용하여 자실체의 유전적 특성을 조사하였다. 그 결과 총 131개의 DNA band가 재현성 있게 나타났다. 또한 산지간의 유연관계를 살펴보기 위하여 Nei의 genetic distance를 이용하여 UPGMA tree를 작성하였다. I-SSR PCR법을 이용하여 DNA를 분석한 결과, 산지간의 유전적 변이는 12.9%에 불과한 반면 산지 내 개체간 변이가 87.1%로 나타났다. 유집 분석 결과는 11개 송이 산지를 제 I 그룹(양양, 함양, 인제, 하동, 울진), 제 II 그룹(남원, 창녕, 청도), 제 III 그룹(고령), 제 IV 그룹(봉화, 괴산)의 4개 그룹으로 분류하였다. 송이의 유전적 다양성, 집단간의 유전적 차이를 나타내는 수치인 Fst 값이 0.13이므로 집단간의 유전적 변이를 어느 정도 나타낸다고 결론짓는다.

• 한국산림과학회지
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• 제89권2호
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• pp.167-172
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• 2000

국내 5개 지역에서 채집한 비자나무(Torreya nucifera Siev. et Zucc.) 95개체를 대상으로 I-SSR 표지자를 분석하였다. 총 62개의 I-SSR 증폭산물(增幅産物)이 관찰되었으며, 그 중 7개의 증폭산물(增幅産物)은 분석된 95개 개체에서 단형성(單形性)이었다. 관찰된 전체 I-SSR 증폭산물(增幅産物)을 통합(統合)하여 분석한 결과 개체목에 대한 DNA지방판별(指放判別)이 가능하였다. 대부분의 유전다양성(遺傳多樣性)이 임분(林分)내의 개체목 간에 존재하는 것으로 나타났고(90.65%), 전체 5개 임분(林分)에서 유사한 수준의 유전다양성(遺傳多樣性)을 보였다. 집단간의 유전적(遺傳的) 분화(分化)정도는 심하지 않았다(${\phi}_{ST}=9.35%$). UPGMA법에 의한 유집분석(類集分析) 결과 각 집단의 유전적(遺傳的) 유연관계(類緣關係)는 임분(林分)의 지리적(地理的) 분포양상(分布樣相)과 일치(一致)하지 않았으며, 각 교점(交點)의 형성(形成)에 있어서 통계적 유의성이 없었고 따라서 전체 집단들이 유전적(遺傳的)으로 크게 분화(分化)되지 않았음을 알 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1355-1359
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• 2004

Japanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p<0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree nformation. Japanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p<0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree information.

• Plant Resources
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• 제8권2호
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• pp.135-144
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• 2005

RAPD analysis was performed to discuss the taxonomic status and phylogenetic relationships among the tribe Forsythieae and related groups. Two hundred and eighteen scorable polymorphic bands were detected from fourteen oligonucleotide primers. From the results of RAPD analysis by Nei and Li's genetic distance, each individuals of Abeliophyllum distichum showed high genetic relationships with ranging from 0.085 to 0.301, also the genus Forsythia showed from 0.042 to 0.655 among the species and populations. But, Abeliophyllum and Forsythia showed distinct dissimilarity, ranging from 0.610 to 1.258. And genetic differences among the population of Forsythia were 0.042 in F. koreana, 0.275 in F. saxatilis, 0.275 in F. ovata, 0.279 in F. nakaii, and 0.249 in F. viridissima. The UPGMA phenogram of tribe Forsythieae based on the results of RAPD analysis were presented that Abeliophyllum is distinct genus different from Forsythia. NJ tree which applied as the outgroups Fontanesia and Jasminum was derived, and it showed that tribe Forsythieae might be a monophyletic group. The genus Fontanesia was showed as sister group of tribe Forsythieae. Among the populations of taxa in Forsythia, F. koreana and F. saxatilis were more closely related, and F. ovata and F. nakaii were very closely related to F.japonica. And Fontanesia was the sister group of tribe Forsythieae.

• 한국발생생물학회지:발생과생식
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• 제24권4호
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• pp.327-335
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• 2020

Genomic DNA (gDNA) extracted from two populations of natural and cultured river pufferfish (Takifugu obscurus) was amplified by polymerase chain reaction (PCR). The complexity of the fragments derived from the two locations varied dramatically. The genetic distances (GDs) between individuals numbered 15 and 12 in the cultured population was 0.053, which was the lowest acknowledged. The oligonucleotide primer OPC-11 identified 88 unique loci shared within each population reflecting the natural population. The OPC-05 primer identified 44 loci shared by the two populations. The average band-sharing (BS) values of individuals in the natural population (0.683±0.014) were lower than in those derived from the cultured population (0.759±0.009) (p<0.05). The shortest GD demonstrating a significant molecular difference was found between the cultured individuals # 15 and # 12 (GD=0.053). Individual # 02 of the natural population was most distantly related to cultured individual # 22 (GD=0.827). A cluster tree was built using the unweighted pair group method with arithmetic mean (UPGMA) Euclidean GD analysis based on a total of 578 various fragments derived from five primers in the two populations. Obvious markers identified in this study represent the genetic structure, species security, and proliferation of river pufferfish in the rivers of the Korean peninsula.

• Mycobiology
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• 제29권4호
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• pp.179-182
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• 2001

Chitin synthases(UDP-N-acetyl-D-glucosamine: chitin 4-$\beta$-N-acetyl-D-glucosaminyl transferase, EC 2.4.1.16) catalyze the synthesis of chitin from UDP-N-acetyl-D-glucosamine. Two zymogenic type of chitin synthase gene(TmCHS1 and TmCHS2) were amplified and its nucleotide sequences were determined. By the amino acid comparison and UPGMA tree grouping, TmChs1 and TmChs2 were classified as class II and class IV chitin synthases respectively. The class II type TmChs1 was grouped with others of Agaricales ectomycorrhizal mushroom. Additionally the phylogenetic tree was well adapted to Hymenomycete previously classified by morphological and physiological characteristics.

• 식물분류학회지
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• 제35권3호
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• pp.161-174
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• 2005

한국산 반하속 식물의 종간 및 종내 집단간의 유연관계를 조사하기 위하여 RAPD 분석을 수행하였으며, PCR 과정을 통해 증폭된 RAPD 절편들은 300 bp에서 2,500 bp 사이의 구간에서 관찰되었다. 7개의 oligoprimer를 이용한 효소중합반응에서 70개의 유효한 polymorphic band makers를 확인하였고, Nei-Li의 유전적 거리지수를 이용하여 분석하였다. 또한 이러한 자료에 근거하여 종내 개체군 군집에 대한 UPGMA 유집분석 및 NJ tree를 도출하였다. 반하의 지역별 개체군 집단간에는 각각 낮은 유전적 거리지수 수준에서 유집되어 전반적으로 개체군간 유연관계가 밀접한 것으로 조사되었다. 반하는 지역별 개체군에 따라 잎의 형태와 꽃의 색에 따른 형태학적 변이 및 체세포염색체수의 세포학적 변이 패턴을 다양하게 보이고 있어 이는 생육지의 다양성에 의해 나타난 형질분화의 차이로 추정된다. 이런 특성은 반하의 분화속도를 빠르게 하는 주요 원인으로 판단된다. 본 연구를 통해 볼 때 새로운 종은 반하속에 속하는 분류군으로 제주도와 일본의 반하 개체군과 매우 가까운 유연관계를 형성하고 있는 것으로 밝혀졌다. RAPD 분석은 한국산 반하속 식물종의 종간 및 종내 개체군 집단간의 유연관계 파악에 매우 유용한 실험적 접근방법임을 보여주었다.

• 한국자원식물학회지
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• 제25권1호
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• pp.72-79
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• 2012

Vaccinium oldhami Miq. is a Korean native tree, which is deciduous and shrub tree with broad leaf. It grows 1~4m in height generally. Ecologically, this tree grows well in shady place even in barren soil. Also, the tree has resistance to cold and dry, which tend to form a little community. This research investigates quantitative morphological characteristics of leaf and fruit among the V. oldhami in South Korea and then considers its relationship on the basis of raw data among the 10 populations. This study will give us invaluable information about growing conditions, reasonable management and breeding by selection of V. oldhami in South Korea. The main results obtained from this study are summarized as follows; Leaf size of Mudeung population was larger than other populations. Naebyeon population was smaller in size of the leaf than other populations. Anmyeondo population was larger in fruit characteristics compared with other populations and Deogyu population was the smallest among populations. According to cluster analysis based on the leaf and fruit morphological characteristics, the natural V. oldhami populations were classified into four groups such as the first group of Kumo population, the second group of Mudeung population, the third group of Anmyundo, Daedun, Doolyun population and the fourth group of the other five populations.

• 한국자원식물학회지
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• 제19권4호
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• pp.509-514
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• 2006

외나로도 식물상을 조사하던 중 분홍색 포를 가진 산딸나무 개체를 발견하였다. 흰색 포를 가지는 일반 산딸나무 개체와 식별가능한 유전자 marker를 탐색하기 위하여 ISSR primer를 사용하였다. 50개의 primer 중 6개 primer에서 총 58개의 증폭산물을 관찰할 수 있었으며, primer당 평균 9.67개가 증폭되었다. UPGMA 방법에 의한 유집분석을 수행한 결과, 산딸나무 개체들은 포가 분홍색인 개체들과 흰색인 개체들이 따로 유집되었으며, 흰색인 개체들은 도서지역 개체들과 내륙지역 개체들로 유집되었다. 산딸나무 화색의 변이에 따른 유전적 다양성조사 결과, 특정 ISSR primer는 꽃이 없는 상태에서 변이 개체를 구분할 수 있는 유용한 marker로 사용될 수 있는 것으로 사려되었다.