• Maxillofacial Plastic and Reconstructive Surgery
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• v.17 no.4
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• pp.331-336
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• 1995

To use cultured mucosa as a graft of full thickness, our laboratory has been involved in the development of techniques to grow epidermis together with connective tissue. Human oral mucosa was obtained at dental surgery. Under sterile conditions the tissues were cut into explants of 0.1 $cm^2$ which were placed in the center of 24 well tissue culture dishes and incubated in a growth medium. The growth medium used for epithelial was MEM(Minimum Essential Medium) supplemented with 10% fetal calf serum, 0.5% dimethyl sulfoxide, glutamine (0.292 g/l), epidermal growth factor (40 ug/ml), cholera toxin (30 ng/ml), hydrocortisone (2 ug/ml), insulin (40 ug/ml) and transferin (5 ug/ml). The medium for stratification of epithelial cells was MEM supplemented with 10% fetal calf serum, 0.5% dimethyl sulfoxide and glutamine (0.292 g/l). The medium used for fibroblasts was MEM supplemented with 10% fetal calf serum. With the three types of media used alternatively, a mucosa composed of epidermis and connective tissue was obtained after 3 weeks of culture.

• Preventive Nutrition and Food Science
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• v.4 no.1
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• pp.43-46
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• 1999

Taurine is known to suppress oxidant-induced tissue injury by stabilizing biomembrane and scavanging free radicals. The purpose of this study was to determne the antioxidative and antimutabenic acitvities of taurine, ad to compare those acitivities with major antioxidants. For the measurement of antioxidative capacity, 0.05 , 0.1,0.5 and 1.0mg/ml of taurine, L-Ascorbic acid, alpha-tocopherol, and BHT (dibuty hydroxiy toluene)were prepared and tested for their ability to donate electrons to DPPH (1,1-diphenyl-2-picryl-hydrazyl). Antimutagenic acitivity was examined using the Ames salmonela test system at concentrations of 600, 900 and 1200ug/ml. Results indicated that taurine possesses electron-donating capacity, however, the degree of donation was very weak compared to the major antioxidants tested. However, taurine was evaluated as a potent mutation suppressor. Antimutagenic capacity was in increasing order BHT>taurine>L-ascorbic acid>alpha-tocopherol at concentrations of 600 and 900ug/ml. There was a dose-dependent increase in antimutabenicity of these compounds , however, antimutagenity of the 900ug taurie/plate was not significantly differently from that of 1200ug taurine/plate. These results indicate that taurine effectively suppresses the mutagenicity of AFB1 without noticeable elelctron donating ability.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.29 no.1
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• pp.123-134
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• 2003

고속액체크로마토그래피 방법을 사용하여 동시에 arbutin, 메탄올에 녹인 methyl, ethyl, prpyl, butyl parben과 glablidien(유용성감초추출물)을 파장 254와 276 nm에서 Gradient methanol로 octdecyl culumn을 사용하여측정하였다. arbutin와 glablidien 농도 0.5-1.0 ug/ml 파라벤류는 0.1-2.0 ug/ml에서 검량선이 직선으로 작성되었다. 검량이 직선으로 나타나 정량분석을 할 수 있다는 것을 알 수 있었다. 샘플 전처리가 크로마토그래피로 측정하기에 좋은 방법이란 것을 판정하기 위하여 일반로션을 사용하여 판정하였다. 이 방법의 정확도는 모든 측정물질(arbutin, methylparaben, ethylparaben, propylparaben, butylparaben, glablidine)의 회수율 상대표준편차(RSD)가 (0.28-2.55%) 나타나 신뢰성 있는 결과를 보였다.A high-performance liquid chromatographic method for the simultaneous determination of arbutine, a mixture of methyl, ethyl, propyl, butyl parabens dissolved in methoanol and Glrablidine(Oil Soluble Licorice), was studied by using a ODS C18 column and a methanol gradient at 254 and 276 nm. Calibration curves were found to be linear in the 0.5-1.0 ug/ml range(compounds arbutine, glrablidin) and 0.1-20 ug/ml (compounds methylpaaben, ethylparaben, propylparaben, butylparaben). Linear regression analysis of the data demonstrates the efficacy of the method in terms of precision and accuracy. An extraction method is developed and validated in order to apply this chromatographic method to a cosmetic lotion. The presision of this method, calculated as the relativ standard deviation(RSD) of the recoveries(0.28-2.55%) was excellent for all compounds.

• Journal of Environmental Health Sciences
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• v.34 no.4
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• pp.285-291
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• 2008

This study was performed to investigate the effects of Chitosan on the nickel poisoning in rats. In the study, 150 male Sprague-Dawley were used. The experimental groups were divided into four: A (30 mg/L nickel), B (30 mg/L nickel+0.2% Chitin, Chitosan and Dithiocarbamate Chitosan), C (30 mg/L nickel+0.4% Chitin, Chitosan and Dithiocarbamate Chitosan), D (30 mg/L nickel+0.8%Chitin, Chitosan and Dithiocarbamate Chitosan). The results were as flows; 1. The nickel concentration in the livers of the control group (A) was $0.153{\sim}0.186\;mg/kg$ but the nickel concentration in the livers of the experimental decreased during the experimental period (P<0.05). 2. Metallothionenin levels in rat liver were $2.77{\sim}3.25\;ug/g$ wet,wt in control group (A), but were $2.89{\sim}3.51\;ug/g$ wet,wt (B), $2.97{\sim}3.62\;ug/g$ wet,wt (C), $2.68{\sim}3.68\;ug/g$ wet,wt (D). Respectively in the experimental groups. The experimental groups were inclined to increase compare to the control group (P<0.05). In conclusion, this study revealed a preventive effect of Chitin, Chitosan and Dithiocarbamate Chitosan against nickel toxicity.

• The Korea Journal of Herbology
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• v.24 no.1
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• pp.151-157
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• 2009

Objectives : The purpose of this study is to investigate the effect of Water Extract from Artemisiae Argi Folium (WAAF) on mouse macrophage Raw 264.7 cells stimulated by lipopolysaccharide (LPS). Methods : Cell viabilities were measured by MTT assay. And the intracellular productions of hydrogen peroxide (H2O2) were measured by dihydrorhodamine 123 assay. TNF-$\alpha$ and IL-6 production from Raw 264.7 were measured by ELISA method. Results : The results of the experiment are as follows. 1. WAAF significantly increased the cell viability compared to the control group (treated with LPS only) at the concentrations of 10, 50, 100, 200, 400 ug/mL. 2. WAAF significantly increased the intracellular production of H2O2 compared to the control group at the concentrations of 50, 100, 200 ug/mL. 3. WAAF significantly decreased the production of TNF-$\alpha$ compared to the control group at the concentrations of 100, 200 ug/mL. 4. WAAF significantly decreased the production of IL-6 compared to the control group at the concentrations of 50, 100, 200 ug/mL. Conclusions : WAAF could be supposed to have the immune-modulating activity related with the macrophage's immunoactivity.

• Journal of Acupuncture Research
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• v.19 no.3
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• pp.41-50
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• 2002

Objective : To study anti-inflammatory, analgesic effect and molecular biological mechanism of honey bee venom for aqua-acupuncture, human mast cell line(HMC-1) and human glioma cell line(HS683) were treated with bee venom. Methods : Cell viability of bee venom was tested by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) asssay. To explore whether anti-inflammatory, analgesic effects of bee venom are associated with the control of gene expression, quantitative RT-PCR analysis of inflammation and pain related genes was performed. Results : The MTT assay demonstrated that cell viability was not decreased by treatment with 10-9 ug/ml bee venom in comparison with 10-2, 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, 10-9, 10-10 and 10-11 ug/ml. sPLA2 and COX-l were down-regulated by treatment with 10-9 ug/ml bee venom in HS683 Cell line in comparison with control. COX-2 was up-regulated by treatment with 10-9 ug/ml bee venom in HS683 Cell line and HSP-2 was up-regulated by treatment with 10-9 ug/ml bee venom in HMC-1 Cell line in comparison with control. sPLA2, COX-1 and COX-2 showed no significant regulation in HMC-1 Cell line and cPLA2 also showed no significant regulation in both HMC-l and HS683 Cell line between control and bee venom treated group.

• Journal of Embryo Transfer
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• v.21 no.2
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• pp.85-93
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• 2006

본 연구는 소 형질 전환 체세포 핵이식에서 용이한 탈핵을 위해 demecolcine을 이용할 시 탈핵율과 핵이식란의 발육능을 높이기 위한 최적의 조건을 알아보고자 실시되었다. 도축장 유래 미성숙 난자를 18시간 체외성숙 후 제1극체가 확인된 성숙 난자를 0.1, 0.2, 0.4 및 0.8 ug/ml의 demecolcine이 첨가된 배지에서 1시간 더 처리한 다음 세포막이 돌출되어 있는 난자를 체세포 핵이식에 공여하여 각 군간 배반포로의 발육능을 비교하였다. Demecolcine 처리 후 핵이 포함된 셰포막의 protrusion rates를 각 군간 비교한 결과 0.2, 0.4 및 0.8 ug/ml 군에서 0.1 ug/ml 군보다 유의적으로 높았으며 (82.8, 86.2, 90.4 vs. 70.1%), conventional blind 방법과 비교한 결과 demecolcine를 이용한 군에서 유의적으로 높은 탈핵율을 보였다(75.3 vs. 96.2%; p<0.05). 체세포 핵이식란의 발육능 비교에서는 0.1 및 0.2ug/ml 군에서 대조군과 함께 유의적으로 높은 분할율 및 배반포로의 발육능을 보였다(p<0.05). 결론적으로 소 형질전환 체세포 핵이식을 위한 탈핵시 높은 탈핵율과 배반포로의 발육율을 얻을 수 있는 demecolcine의 적정농도는 0.2ug/ml이라고 사료된다.

• Journal of Haehwa Medicine
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• v.20 no.2
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• pp.53-65
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• 2012

In order to investigate the possibility of SHT as therapeutic for the treatment of atopic dermatitis (AD), cytotoxicity, anti-oxidant activity, modulatory and suppression activities of SHT were tested. 90% or higher cell viability was observed in all tested groups from 25 to 200 ug/ml using Raw 264.7 cells. SHT showed dose-dependent DPPH scavenging activity, with more than 80% scavenging activities at 400 and 800 ug/ml concentrations. SHT showed dose-dependent suppression activity of ROS production, especially at 200 ug/ml of 57.4%. SHT decreased NO production activity dose dependently, expecially at 200 ug/ml of 28.8%. IL-$1{\beta}$, IL-6, MCP-1, TNF-${\alpha}$ production rate were decreased by 45.7%, 15.5%, 8.9%, 16.5% respectively when Raw 264.7 cells were treated with LPS and with SHT of 200 ug/ml. However, only IL-6 and TNF-${\alpha}$ showed significant changes. The results above indicate that SHT significantly reduces the effect of oxidative and inflammatory cytokines. The use of SHT in dermatitis can be widely suggested.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1349-1357
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• 2009

This study was performed to investigate the effects of Shigyungbanha-tang(SGT) on the lipopolysaccharide(LPS) induced acute lung injury(ALI) in mice. 1 and 24 h before LPS intratracheal instillation, control group was taken distilled water orally. Treated groups was taken each concentrate SGT(2.5 g/kg, 6.7 g/kg) by orally as same times. Normal group was not instilled with LPS and was taken distilled water. 24 h after LPS intratracheal instillation, lung histology was performed in inflated-fixed lungs in 3 mice of each groups. The other mice of each groups, bronchoalveolar lavege fluids(BALF) was obtained to measure proinflammatory cytokines(TNF-$\alpha$, IL-$1{\beta}$, IL-6) and blood sample was obtained to measure white blood cell(WBC). In vitro, the effect of SGT($100\;ug/m{\ell}$, $500\;ug/m{\ell}$, $1000\;ug/m{\ell}$) on the release of RANTES, TARC induced by TNF-$\alpha$ and IL-4 in human alveolar epithelial cell(A549) was examined. Histopathologically, SGT prevented LPS-induced lung injury. SGT decreased protein, TNF-$\alpha$, IL-$1{\beta}$ and IL-6 according to concentrations. In vitro, $500\;ug/m{\ell}$, $1000\;ug/m{\ell}$ concentrate SGT suppressed the expression of RANTES and TARC on A549 cells. On the basis of these results, SGT had a markedly anti-inflammatory effect in a clinically relevant model of ALI. Nevertheless, further investigations are required to determine the potential clinical usefulness of SGT in the adjunctive therapy of ALI.

• Korean Journal of Community Nutrition
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• v.5 no.2
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• pp.289-296
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• 2000

The purpose of this study was to investigate the relationship and lipids in healthy adults on self-selected diets. These subjects consisted of 40 female college students residing in Chungnam. Anthropometric measure-ments, diet intake measurements, and blood collection were conducted. Serum concentrations of 5 microminerals(As, Cr, Mn, Se, Ni), lipids(triglyceride, total cholesterol, HDL-cholesterol), and glucose were measured by an ICP spectrometer and biochemical analyzer. The results were as follows. The mean age of the subjects was 22.34 years and the mean weight, height, and BMI were 52.89kg, 161.29cm and 20.34, respectively. The mean serum concentrations appeared to be 14.60ug/dl(As), 1.87ug/dl(Cr), 0.18ug/dl(Mn), 23.50ug/dl(Se), 0.21ug/dl(Ni), 60.73mg/dl(triglyceride), 138.49mg/dl(total cholesterol), 65.95mg/dl(HDL-cholesterol), 60.39mg/dl(LDL-cholesterol) and 88.82mg/dl(glucose). When analyzed by Pearson’s correlation coefficient, the serum concentration of Cr was negatively correlated with Ca and vitamin B12 intake(p〈0.05, p〈0.05) respectively, Mn was negatively correlated with Na intake(p〈0.05), Ni, however, was positively correlated with K intake(p〈0.05). The serum concentration of Se was positively correlated with LDL-cholesterol(p〈0.05), Ni, however, negatively correlated with total cholesterol, HDL-cholesterol, LDL-cholesterol, and glucose, respectively(p〈0.001, p〈0.01, p〈0.05). Further studies are needed to clarify the precise micromineral intakes, nutritional assessment of microminerals, and cause-effect relation of microminerals and serum lipids.