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Effects of Shigyungbanha-Tang on the Lipopolysaccharide-Induced Acute Lung Injury in Mice  

Kim, Ki-Tae (Department of Internal Medicine, College of Oriental Medicine, Semyung University)
Ko, Heung (Department of Internal Medicine, College of Oriental Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.6, 2009 , pp. 1349-1357 More about this Journal
Abstract
This study was performed to investigate the effects of Shigyungbanha-tang(SGT) on the lipopolysaccharide(LPS) induced acute lung injury(ALI) in mice. 1 and 24 h before LPS intratracheal instillation, control group was taken distilled water orally. Treated groups was taken each concentrate SGT(2.5 g/kg, 6.7 g/kg) by orally as same times. Normal group was not instilled with LPS and was taken distilled water. 24 h after LPS intratracheal instillation, lung histology was performed in inflated-fixed lungs in 3 mice of each groups. The other mice of each groups, bronchoalveolar lavege fluids(BALF) was obtained to measure proinflammatory cytokines(TNF-$\alpha$, IL-$1{\beta}$, IL-6) and blood sample was obtained to measure white blood cell(WBC). In vitro, the effect of SGT($100\;ug/m{\ell}$, $500\;ug/m{\ell}$, $1000\;ug/m{\ell}$) on the release of RANTES, TARC induced by TNF-$\alpha$ and IL-4 in human alveolar epithelial cell(A549) was examined. Histopathologically, SGT prevented LPS-induced lung injury. SGT decreased protein, TNF-$\alpha$, IL-$1{\beta}$ and IL-6 according to concentrations. In vitro, $500\;ug/m{\ell}$, $1000\;ug/m{\ell}$ concentrate SGT suppressed the expression of RANTES and TARC on A549 cells. On the basis of these results, SGT had a markedly anti-inflammatory effect in a clinically relevant model of ALI. Nevertheless, further investigations are required to determine the potential clinical usefulness of SGT in the adjunctive therapy of ALI.
Keywords
Shigyungbanha-tang; lipopolysaccharide; TNF-$\alpha$; IL-$1{\beta}$; IL-6; RANTES; TARC; ALI;
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1 서울대학교의과대학 내과학교실. 임상 내과학(I). 서울, 고려의학, pp 1076-1089, 2004
2 김성은, 이영만, 박원학. 내독소로 유도된 급성폐손상에서 PLA2의 억제제인 Rutin의 효과. Korean J. Electron Microscopy 34(1):31-42, 2004   과학기술학회마을
3 Vadas, P., Browning, J., Edelson, J., Pruzanski, W. Extracellular phospholipase A2 expression and inflammation. Journal of Lipid Mediators 8: 1-8, 1993   PUBMED
4 권 현, 정승기, 이형구. 시경청폐탕과 시경반하탕이 Xylene으로 인한 흰쥐의 폐부종에 미치는 영향. 경희의학, 5(3):337-345, 1989
5 Lewis, J.F., McCaig, L. Aerosolized versus instilled exogenous surfactant in nonuniform pattern of lung injury. Am Rev Respir Dis 148: 1187-1193, 1993   DOI   PUBMED   ScienceOn
6 Articas, A., Bernard, G.R., Carlet, J., Dreyfuss, D., Gattinoni, L., Hudson, L., Lamy, M., Marinni, J.O., Matthay, M.A., Pinsky, M.R., Spracc, R., Suter, P.M. and the Consensus Committee. The American-European Consensus Conference on ARDS, Part 2. Am J Respir Crit Care Med 157: 1337-1347, 1998
7 M. Bachofen, E.R. Weibel. Structural alterations of lung parenchyma in the adult respiratory distress syndrome. Clinics in Chest Medicine 31: 35-56, 1982
8 李梴. 醫學入門. 서울, 大星文化社, p 208, 1984
9 Chia Chou Yeh, Chih Che Lin, Shulhn Der Wang, Che Ming Hung, Ming Hsien Yeh, Ching Ju Liu, Shung Te Kao. Protective and immunomodulatory effect of Gingyo-san in a murine model of acute lung inflammation. Journal of Ethnopharmacology 111(2):418-426, 2007   DOI   ScienceOn
10 Alam, R., et al. RANTES is a chemotactic and activating factor for human eosinophils. Journal of Immunology 150: 3442-3448, 1993
11 신원용, 현민경, 정병무, 최은영, 윤철호, 정지천. 노인성 폐렴환자 1례에 대한 증례보고. 대한한방내과학회지 26(1):229-235, 2005   과학기술학회마을
12 郭子光 外. 現代中醫治療學. 四川, 四川科學技術出版社, pp 92-94, 1995
13 Kahdi, F., Udobi, E.D., Childs Karim Touijer. Acute Respiratory Distress Syndrome. AMERICAN FAMILY PHYSICIAN 67(20):315-322, 2003
14 Mandana, M., Mojtaba, M., Ali, M., Mohammad, S.S., Atabak, N., Mohammad, R.K., Molook, H., Mohammad, H.G. The role of glutathione-S-transferase polymorphisms on clinical outcome of ALI/ARDS patient treated with N-acetylcysteine. Respiratory Medicine 1: 1-8, 2008   DOI   ScienceOn
15 Behnia, R., Molteni, A., Waters, C.M., Panos, R.J., Ward, W.F., Schnaper, H.W. Early markers of ventilator-induced lung injury in rats. Ann Clin Lab Sci 26: 437-450, 1996   PUBMED
16 Chia Chou Yeh, Shang Jyh Kao, Chih Che Lin, Shulhn Der Wang, Ching Ju Liu, Shung Te Kao. The immunomodulation of endotoxin-induced acute lung injury by hesperidin In vivo and In vitro. Life Sciences 80(20):1821-1831, 2007   DOI   ScienceOn
17 Hyungug Cho. Germanium increases alveolar macrophage engulfment of apoptotic neutrophils in acute lung injury induced by endotoxin. Korean J. Electron Microscopy 35(4):1-7, 2005
18 김선영, 김창환, 김태규, 김희제, 서영훈, 성정석, 엄현석, 이기현 외. 면역학 제2판. 서울, 라이프사이언스, pp 223-273, 2006
19 윤호주. 급성 폐손상에서 cytokine의 역할. 대한내과학회지 54(4):544-549, 1999
20 Van der Poll, T., Keogh, C.V., Guirao, X., Buurman, W.A., Kopf, M., Lowry, S.F. Interleukin-6 gene-deficient mice show impaired defense against pneumococcal pneumonia. J Infect Dis 176(2):439-444, 1997   DOI   ScienceOn
21 이경진, 김재홍, 문상관, 조기호, 배형섭, 이경섭. 뇌졸중의 폐렴합병증에 대한 시경반하탕의 치험1예. 경희의학 15(1)107-112, 1999
22 이상민, 유철규. 급성호흡곤란증후군. 대한내과학회지 68(5): 476-486, 2005
23 이승언, 신조영, 이시형. LPS로 유도한 복강대식세포에서 IκBa 분해억제에 의한 柴梗半夏湯의 항염증효과. 대한한방내과학회지 28(3):442-452, 2007
24 윤용갑. 동의방제와 처방해설. 서울, 의성당, pp 639-692, 2002
25 Hierholzer, C., Kalff, J.C., Omert, L., Tsukada, K., Loeffert, J.E., Watkins, S.C., et al. Interleukin-6 prodection in hemorrhagic shock is accompanied by neutrophil recruitment and lung injury. Am J Physiol 275: 611-621, 1998
26 전국한의과대학 본초학교실. 본초학. 서울, 영림사, pp 136-541, 2000
27 Xiao Su, Yuanlin Song, Jinjun Jiang, Chunxue Bai. The role of aquaporin-1(AQP1) expression in a murine model of lipopolysaccharide-induced acute lung injury. Respiratory Physiology & Neurobiology 142(1):1-11, 2004   DOI   ScienceOn
28 Imai, T., et al. The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. Journal of Biological Chemistry 272(23):15036-15042, 1997   DOI   ScienceOn
29 정진홍, 신경철. Interleukin-1으로 유도된 흰쥐 급성폐손상에서 Aerosolized Vitamin-E의 전처치 효과. 영남의대학술지 24(2):365-372, 2007
30 대한내과학회 해리슨내과학 편집위원회. Harrison's Principles of Internal medicine 16th edition. 서울, MIP, pp 1737-1740, 2006
31 김성호, 김희택. 황금이 A549 세포주에서 TNF-a 및 IL-4로 유도된 chemokines에 미치는 영향. 한방안이비인후피부과학회지 20(2):108-115, 2007
32 김민희, 조승제, 송준섭, 노창수, 정헌종. 내독소 투여로 유발된 폐손상에서 표면활성제 투여 방법이 IL-1$\beta$와 IL-6치에 미치는 효과. 대한주산의학회지 15(2):154-163, 2004
33 Katsumi Shimoda, Hiroaki Nakazawa, Maret, G., Traber, Daniel, L. Traber, Motohiro Nozaki. Plasma and tissue vitamin E depletion in sheep with burn and smoke inhalation injury. burns 34: 1137-1141, 2008   DOI   ScienceOn
34 전국한의과대학 폐계내과학교실. 동의폐계내과학. 서울, 나도, pp 72-74, 90, 192-199, 2007