• Journal of Microbiology and Biotechnology
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• 제20권9호
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• pp.1314-1321
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• 2010

Sulfur metabolism in S. cerevisiae is well established, but the mechanisms underlying the formation of sulfide remain obscure. Here, we investigated by real-time RT-PCR the dependence of expression levels of MET3, MET5/ECM17, MET10, MET16, and MET17 along with SSU1 on nitrogen availability in two wine yeast strains that produce divergent sulfide profiles. MET3 was the most highly expressed of the genes studied in strain PYCC4072, and SSU1 in strain UCD522. The strains behaved differently according to the sampling times, with UCD522 and PYCC4072 showing the highest expression levels at 120 h and 72 h, respectively. In the presence of 267 mg assimilable N/l, the genes were more highly expressed in strain UCD522 than in PYCC4072. MET5/ECM17 and MET17 were only weakly expressed in both strains under any condition tested. MET10 and SSU1 in both strains, but MET16 only in PYCC4072, were consistently upregulated when sulfide production was inhibited. This study illustrates that strain genotype could be important in determining enzyme activities and therefore the rate of sulfide liberation. This linkage, for some yeast strains, of sulfide production to expression levels of genes associated with sulfate assimilation and sulfur amino acid biosynthesis could be relevant for defining new strategies for the genetic improvement of wine yeasts.

• 한국게임학회 논문지
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• 제14권5호
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• pp.137-146
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• 2014

최근 게임시장은 게임제작과정과 게임디자인 분야의 많은 발전에 의하여 게임 상 컷씬(cut-scene)의 디자인 스타일과 완성도 또한 지속적인 발전을 이루고 있다. 따라서 항상 게임 디자이너들은 여러 디자인 방향성에서 보다 쉬운 인지와 인기 있는 디자인을 얻으려는 노력을 한다. 그러나 개발자에 의존한 디자인 전략은 최종 디자인결과물이 게임플레이어에게 사랑 받고 있는지 성과여부를 판단 할 수 없게 되며 모든 면에서 빠르게 변화하는 게임시장의 요구를 충족 할 수 없다. 또한 디자인 최종 완성도와 같은 어려운 절차들은 게임 컷씬 장면의 제작 및 이용을 어렵게 한다. 따라서 본 연구는 사용자 중심 디자인(UCD) 바탕의 비디오 게임 속 컷씬 디자인방법을 제시하여 제작환경에서 원작의 느낌을 훼손하지 않으면서 디자이너가 전하고자 하는 메시지를 더욱 부각시키고 흥미를 이끌어 몰입감을 높이는데 도움이 될 것이다.

• 한국식품저장유통학회지
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• 제15권2호
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• pp.306-310
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• 2008

탐나라, M.B.A 두 포도품종을 이용하여 건조 효모 S. cerevisiae Montrachet(UCD #522), S. bayanus Pasteur Champagne (UCD #595), S. cerevisiae Epernay II (CEG) 3종을 첨가하여 포도주 발효 특성과 관능적 검사를 비교하였다. 포도 두 품종을 $15^{\circ}C$에서 1차 발효시킨 결과 발효과정중 당함량이 감소되고 알코올이 증가되는 발효 형태 및 발효 속도에는 큰 차이가 없었으나 두 품종 모두 효모 첨가로 인해 발효 속도는 효모 종류에 관계없이 매우 빨라졌다. 30일간의 1차 발효 후 $10^{\circ}C$에서 60일 발효하여 관능검사를 실시한 결과 효모첨가여부에 관계없이 M.B.A가 탐나라보다 기호도가 상대적으로 높았으며 두 품종 모두 효모 첨가구의 기호도가 유의적인 차이는 없었으나 효모를 첨가하지 않은 것보다 낮게 나타났다. 전자혀를 이용하여 측정한 맛의 분포도에 있어서 탐나라와 M.B.A 두 포도품종 간에는 차이가 있었으나 효모균주 간에는 차이가 없음을 보여주어 기계적인 측정과 관능검사의 결과가 동일함을 보여주었다.

• 한국환경보건학회지
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• 제49권1호
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• pp.48-56
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• 2023

Background: Few studies have assessed exposure to chemicals in the context of environmental vulnerability with a focus on exposure among populations living in certain geographical areas. Objectives: This study aimed to investigate cadmium exposure levels and kidney damage indices in environmentally and socioeconomically vulnerable populations, with further subgrouping according to economic status. Methods: Four areas were selected to represent geographical vulnerability (two environmentally vulnerable populations and two socioeconomically vulnerable populations). Among them, population groups with lower socioeconomic status (SES) were separately classified. Urinary cadmium (UCd), beta2-microglobulin (β2-MG), and N-acetyl-β-D-glucosaminidase (NAG) levels were analyzed in samples from 245 residents of these four areas. Results: Geometric means of concentrations of UCd (0.97~2.02 ㎍/g creatinine) in all selected populations (N, 245; mean age, 67.8~70.9 years old) were higher than the national reference values (0.39 for adults and 0.78 ㎍/g creatinine for people in their 60s). Participants with a lower SES had higher UCd and NAG concentrations than did non-low SES participants. In the lower SES group, there was a significant association between UCd and NAG concentrations; however, there was no such correlation in the non-low SES group. Conclusions: Consistent with the findings of previous studies evaluating chemical exposure and associated health effects in specific populations, the findings of this study suggest that individuals with a low SES may be more vulnerable to exposure and related health effects.

• 드라이브 ㆍ 컨트롤
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• 제5권4호
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• pp.2-49
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• 2008

회사/제품/신기술정보: 두산모트롤(주), (주)보쉬 렉스로스 코리아, 하베코리아(주), 코리아유씨디(주), 한국유켄공업(주), 파크전자(주)

• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1125-1131
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• 2006

A kan'::luxCDABE fusion strain that was both highly bioluminescent and responsive to benzoic acid was constructed by transforming E. coli strain W3110 with the plasmid pUCDK, which was constructed by digesting and removing the 7-kb KpnI fragment from the promoterless luxCDABE plasmid pUCD615. Experiments using buffered media showed that this induction was dependent on the pH of the media, which influences the degree of benzoic acid protonation, and the expression levels seen are likely due to acidification of the cytoplasm by uncoupling of benzoic acid. Consequently, the sensitivity of this strain for benzoic acid was increased by nearly 20-fold when the pH was shifted from 8.0 to 6.5. Benzoic acid derivatives and several phenolics also resulted in significantly increased bioluminescent signals. Although these compounds are known to damage membranes and induce the heat-shock response within E. coli, bacterial strains harboring mutations in the fadR and rpoH genes, which are responsible for fatty acid biosynthesis during membrane stress and induction of the heat-shock response, respectively, showed that these mutations had no effect on the responses observed.

• 한국식물병리학회지
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• 제14권1호
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• pp.13-18
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• 1998

The use of bioluminescence as a sensitive marker for the detection of Pseudomnas sp. in the rhizosphere was investigated. Transposon Tn4431 which contains a promoterless luciferase operon and tetracycline resistant gene was used. This transposon, present on a suicide vector (pUCD623) in E. coli HB101, was mated with spontaneous rifampicin mutant of Pseudomonas fluorescens B16, a plant growth promoting rhizobacteria (PGPR), and then rifampicin and tetracycline resistant survivors were isolated. Twenty tow mutants wer isolated from the conjugants between E. coli HB101 and P. fluorescens B16. One of these, B16::Tn4431 (L22) recombinant which glowed brightly in the dark was selected for analysis. The cucumber seeds inoculated with L22 were grown in moisten two layers of filter paper and nonsterile soil contained in half cut PVC pipe. The roots were removed from the filter paper and PVC pipe, then placed on the 1/2 LB media plates. The plates were incubated at room temperature for 16 hr. L22 could successfully be detected in the rhizoplane by using the ordinary negative camera film (ASA100-400) with 30 minutes exposure under dark condition. The root colonizing ability and the plant growth promoting effect of L22 were not reduced compared to the untreated bacteria and wild type. L22 was superior to will type.

• 한국멀티미디어학회논문지
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• 제22권2호
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• pp.322-333
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• 2019

After the launch of smart phones, various miniaturized smart devices such as wearable and IOT devices have deeply embedded in human life, and have created a technology-oriented society. In this technology-oriented society, technology development itself is important, however it seems more important to utilize existing technology appropriately and deliver effectively to human life. As the computer became personalized after the appearance of PC, human-centered computing such as HCI and UCD had begun to appear. However, most of the researches focused on technology that made human being convenient to interact with computer such as computer systems design and UX development. In the technology-oriented society, it seems more urgent to apply existing technology to human life. In this paper, we propose a methodology, 'Digital Leveraging' which guides how to effectively apply technology to human life. Digital Leveraging is the way of convergence between technology and humanities.

• 방사성폐기물학회지
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• 제11권3호
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• pp.199-206
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• 2013

파이로프로세싱에서 전해제련은 액체카드뮴음극(liquid cadmium cathode, LCC)을 이용하여 우라늄과 초우라늄원소(TRU)를 동시에 회수하는 공정이다. 액체카드뮴음극의 표면에 전착된 우라늄이 카드뮴 중의 우라늄 용해도(2.35wt%)를 초과하여 전착되면, 표면적이 큰 수지상 우라늄을 형성하여 액체카드뮴 내부로 가라앉지 않고 이 수지상 우라늄 자체가 고체전극으로 작용한다. 따라서 본 연구에서는 Cd-U 상태도를 바탕으로 ${\alpha}$상 우라늄(수지상 우라늄)이 안정하게 존재하는 $500^{\circ}C$와 카드뮴과 우라늄간 금속간 화합물(intermetallic compound)이 형성되는 $440^{\circ}C$의 두 가지의 온도 조건에서 전착실험을 하였다. $440^{\circ}C$에서 정전류법으로 전착한 경우, 우라늄은 수지상이 아닌 알갱이 형태로 전착되었고 액체카드뮴음극의 도가니 밖으로 자라나지 않은 채 카드뮴 풀 중앙을 중심으로 일정하게 적층되었다. XRD 분석을 통해 이러한 전착물이 $UCd_{11}$이라는 금속간 화합물이라는 것을 알 수 있었다. $UCd_{11}$은 카드뮴보다 비중이 커서 전착 중에 액체카드뮴 내부로 침전되므로 교반기를 사용하지 않고도 우라늄과 초우라늄원소를 동시에 회수할 수 있을 것으로 판단된다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 하계종합학술대회 논문집(1)
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• pp.231-234
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• 2002

This paper has studied a management system for RCST of the Satellite Multimedia Internet Service through analysing the architecture and specification of BSAN based on Digital Video Broadcasting System. In this paper, designing and implementing RCST DPM and agent system are shown. The UCD-SNMP framework served as base station for implementation of the agent. And the message protocol between RCST DPM and agent system has been also defined to provide a way for interaction.