• 생명과학회지
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• 제19권11호
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• pp.1514-1521
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• 2009

혈장 cathepsin B 활성도 측정이 갑상선암 및 결절성 증식증의 수술 전 진단에 도움이 되는지를 알아보고자 분화갑상선암 32례, 결절성 증식증 7례, 대조군 5례의 혈장 cathepsin B 발현양상을 대조군과 함께 관찰하였다. 수술시 제거된 여포선암의 암조직, 결절성 증식증 조직의 cathepsin B 발현을 정상 조직과 비교하였다. 혈장 Cathepsin B 활성도는 정상 대조군에서 $168.94{\pm}15.10$ (${\times}10^{-2}$, mU), 결절성 증식증에서 $255.45{\pm}95.68$ (${\times}10^{-2}$, mU), 악성종양에서 $284.87{\pm}79.32$ (${\times}10^{-2}$, mU)로써 악성종양군과 결절성 증식증군에서 cathepsin B 발현이 정상 대조군보다 비교적 높게 나타났다(p<0.05). 혈장 cathepsin B의 정량적 비교를 위한 immunoassay결과에서도 결절성 증식증군($17.64{\pm}7.49\;ng/ml$)과 악성종양군($15.50{\pm}7.86\;ng/ml$)에서 정상대조군($4.85{\pm}0.61\;ng/ml$)보다 높은 수치를 나타내었다(p<0.05). 결절성 증식증 및 악성종양군의 조직 내 cathepsin B mRNA발현이 정상 조직에서보다 높게 나타났다. 따라서 혈장 cathepsin B는 갑상선세포의 비정상적인 증식시에 증가됨을 알 수 있으며, 갑상선암 혹은 결절성 증식증을 스크리닝하는데 이용될 수 있을 것으로 사료된다.

• 생태와환경
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• 제41권3호
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• pp.402-411
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• 2008

남조류 제어능이 밝혀진 국내산 말조개와 논우렁이를 금강수계에서 채집하여, 저온기 부영양 호수에서 우점하는 규조류에 대한 섭식특성을 파악하고자, 두 패류를 단독 또는 혼합적용하고, 수조내 엽록소-a, 조류현존량, 섭식율 및 배설물 생산량의 변화를 각각 조사하였다. 섭식 실험은 패류밀도에 따라 말조개 단독처리군 5가지, 논우렁이 단독처리군 5가지, 두 패류의 혼합 처리군 4가지 등으로 구분하여 각각 3회씩 반복 실험하였다. 분석결과, 모든 패류 처리군에서 엽록소-a (또는 조류현존량 농도)가 뚜렷하게 감소하였으며, 말조개와 말조개 밀도가 높은 혼합군에서 조류제어율이 높았다. 각 출현종에 대한 패류의 조류제어능은 Synedra ulna, Scenedesmus sp., Ankistrodesmus falcatus 등은 말조개 밀도가 높을수록, Diatoma vulgare는 혼합 처리군에서 각각 높게 제어된 반면, Cryptomonas ovata는 고밀도 논우렁이 처리군에서 오히려 20% 성장을 유도하였다. 패류의 섭식율은 패류와 처리시간에 따라 차이를 보였는데, 초기에는 운동성이 좋은 논우렁이가 강한 섭식을 보인 반면, 시간이 경과함에 따라 말조개 및 고밀도 말조개 혼합 처리군에서 섭식율이 높았다. 패류의 배설물 생산은 혼합 처리군에서 가장 높았으며, 60 ${\mu}m$ 이상의 크기에서 높게 나타났다. 본 연구에서 국내산 말조개와 논우렁이를 단독 또는 혼합 적용시 모든 처리군에서 규조류가 뚜렷하게 감소하였으며, 말조개나 말조개 혼합 비율이 높을수록 제어율이 높았다. 따라서 앞으로 패류들의 현장적용 기술이 개발된다면 저온기 규조류 대발생의 생태친화적 제어가 가능할 것으로 사료되었다.

• 말소리와 음성과학
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• 제9권4호
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• pp.35-41
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• 2017

The existence and the extent of vowel length difference before voiced/voiceless consonants in English and Korean are examined in three groups: (1) Korean-speaking Americans (group A), (2) immigrants who moved to the U.S. in their early teens (group I), and (3) Koreans who have been in the U.S. for less than 3 years (group K). 14 subjects were recorded reading 10 English and 10 Korean sentences. The results show that the three groups exhibit different patterns of the vowel length difference: Group A shows a very strong tendency of vowel lengthening before voiced consonants in both English and Korean, while Group I shows less degree of vowel lengthening, and Group K shows almost no tendency of vowel length difference in both languages. This strongly suggests that, (1) unlike English, Korean does not have the vowel length difference depending on the following consonants, and (2) the vowel lengthening effect observed in Korean (L2) speech in group A may be the result of transfer of the phonetic trait acquired in English (L1). It also implies that, in teaching pronunciation, some facts such as the vowel length difference cannot be expected to be acquired automatically for the learners of English, but have to be taught explicitly.

• Journal of Oral Medicine and Pain
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• 제44권1호
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• pp.11-15
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• 2019

Purpose: The aim of this study was to compare the potency-stabilizing effects of two different diluents of botulinum toxin A (10% dextrose solution and 0.9% saline). Methods: A mouse lethality bioassay was undertaken. Ninety mice were divided into experimental and control groups which received varying dosages in subgroups of 10. The experimental group was injected with botulinum toxin A diluted with 10% dextrose solution and the control group was injected with botulinum toxin A diluted with 0.9% saline. A 72 hours after intraperitoneal injection, the number of dead mice was counted to confirm median lethal dose ($LD_{50}$) of each group. Results: The value of $LD_{50}$ in the experimental group was approximately 0.131 mL (1.31 U) and the value of $LD_{50}$ in the control group was approximately 0.107 mL (1.07 U). The potency preservation rate of the experimental group was estimated to be 93.5% and that of the control group was estimated to be 76.3%. Conclusions: Dilution with 10% dextrose solution displayed less potency loss than 0.9% saline.

• 수목보호
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• 통권10호
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• pp.41-48
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• 2005

• the USERS
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• 통권45호
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• pp.8-11
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• 2005

• the USERS
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• 통권47호
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• pp.34-34
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• 2006

• 대한치과교정학회지
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• 제29권3호
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• pp.317-325
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• 1999

골격성 III급 부정교합 환자의 정확한 진단과 분석에 있어서 삼차원적인 골격부조화의 양상이나 안면부조화의 원인을 파악하는 것은 중요하다. 본 연구에서는 정모 두부규격방사선 사진 상에서 나타나는 골격성 III급 부정교합자의 계측학적 특성을 파악하기 위하여, 양호한 안모와 교합을 가지는 성인 남녀 정상교합자 60명과 전후방적 부조화가 심한 III급 부정교합을 가지는 성인 남녀 60명을 대상으로 측모와 정모 두부규격방사선 사진의 투사도를 작성하여 안면폭경, 고경, 각각의 비율, 측모계측치에 대응되는 정모계측치의 비율을 구하였고, 각각의 계측치와 비율을 비교, 분석하여 다음과 같은 결론을 얻었다. 1. 골격성 III급 부정교합자에서 골격의 전후방적 부조화는 정상군보다 상악골길이(Cd-A)가 짧은 것보다는 하악골길이(Cd-Gn)가 긴 것에 기인하였다. 2. 골격성 III급 부정교합자는 정상교합자보다 긴 안모를 가지는데, 이는 상안면고경(Cg-ANS)보다는 하안면 고경(ANS-Me), 특히 하악골 고경(Cd-Me)의 증가로 인한 것이었다. 3. 골격성 III급 부정교합자의 폭경은 단지 여자의 상$\cdot$하악 대구치간 폭경(U6-U6, L6-L6)과, 하악폭경(Ag-Ag)만 정상교합자보다 컸을 뿐, 이외 어떤 폭경항목도 정상과 차이가 없었다. 4.골격성 III급 부정교합자의 하악골 길이의 증가는 안모의 고경 특히 하안면 고경의 증가로 나타나지만, 하악골 폭경에서는 영향이 나타나지 않았다.

• Journal of Korean Neurosurgical Society
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• 제46권4호
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• pp.292-299
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• 2009

Objective : Clinical and radiological results of posterior dynamic stabilization using interspinous U (ISU, $Coflex^{TM}$, Paradigm Spine $Inc.^{(R)}$, NY, USA) were analyzed in comparison with posterior lumbar interbody fusion (PLIF) in degenerative lumbar spinal stenosis (LSS). Methods : A retrospective study was conducted for a consecutive series of 61 patients with degenerative LSS between May 2003 and December 2005. We included only the patients completed minimum 24 months follow up evaluation. Among them, 30 patients were treated with implantation of ISU after decompressive laminectomy (Group ISU) and 31 patients were treated with wide decompressive laminectomy and posterior lumbar interbody fusion (PLIF; Group PLIF). We evaluated visual analogue scale (VAS) and Oswestry Disability Index (ODI) for clinical outcomes (VAS, ODI), disc height ratio disc height (DH), disc height/vertebral body length ${\times}100$), static vertebral slip (VS) and depth of maximal radiolucent gap between ISU and spinous process) in preoperative, immediate postoperative and last follow up. Results : The mean age of group ISU ($66.2{\pm}6.7$ years) was 6.2 years older than the mean age of group PLIF ($60.4{\pm}8.1$ years; p=0.003). In both groups, clinical measures improved significantly than preoperative values (p<0.001). Operation time and blood loss was significantly shorter and lower in group ISU than group PLIF (p<0.001). In group ISU, the DH increased transiently in immediate postoperative period ($15.7{\pm}4.5%{\rightarrow}18.6{\pm}5.9%$), however decreased significantly in last follow up ($13.8{\pm}6.6%$, p=0.027). Vertebral slip (VS) of spondylolisthesis in group ISU increased during postoperative follow-up ($2.3{\pm}3.3{\rightarrow}8.7{\pm}6.2$, p=0.040). Meanwhile, the postoperatively improved DH and VS was maintained in group PLIF in last follow up. Conclusion : According to our result, implantation of ISU after decompressive laminectomy in degenerative LSS is less invasive and provides similar clinical outcome in comparison with the instrumented fusion. However, the device has only transient effect on the postoperative restoration of disc height and reduction of slip in spondylolisthesis. Therefore, in the biomechanical standpoint, it is hard to expect that use of Interspinous U in decompressive laminectomy for degenerative LSS had long term beneficial effect.

• 대한한방내과학회지
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• 제25권3호
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• pp.427-439
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• 2004

It has been reported that Bopheyangyoungjeon(BYJ) has an effect on deficiency asthma(喘虛) clinically. The aim of this study was to determine an appropriate dosage of BYJ to treat asthma. In order to study the effects of orally administered BYJ on allergic asthma, mice were pretreated with three oral doses of the herbal solution of BYJ before antigen sensitization. 2 days later Mice were actively sensitized with a subcutaneous injection of ovalbumin and 13 day later ovalbumin aerosols were used to provoke asthmatic reaction. Serum level of IgE, IL-4, WBC, RBC, HGB, cell numbers in bronchoalveolar lavage fluid(BALF), and in vitro isometric contractile responses of the isolated tracheal smooth muscle(TSM) to acetylcholine(ACh, 0.1-1000uM), KCl were measured. The results were as follows ; 1. Contractile responses of TSM to ACh significantly increased in C group at Ach 0.3, 1, 3, 10, 30, 100, 300, 1000uM(P<0.05, P<0.01) and increased in D at 0.1, 0.3, 3, 30, 30, 100, 300, 1000uM. 2. The sensitivity of TSM to Ach increased more in A, B group, but it was not significant. 3. The maximal contractile response of TSM to ACh decreased more significantly in C group(P<0.01) and D group(P<0.05) the control group. 4. The maximal contractile response of TSM to KCI decreased more significantly in B group and C group(P<0.001) than in the control group. 5. The counts of lymphocytes in BALF decreased more significantly in B group and D group(P<0.05) than in the control group. 6. The counts of macrophages in BALF decreased more significantly in B group, C(P<0.05) than in the control group. 8. Serum IgE level increased more significantly in B group and C group(P<0.05) than the control group. 9. The counts of WBC, RBC, HGB in blood increased more significantly in A group than the control group. The above results support a role for BYJ orally administered in treatment of deficiency allergic Asthma.