• Annals of Clinical Neurophysiology
• /
• 제7권1호
• /
• pp.25-27
• /
• 2005

The Guillain-Barre syndrome (GBS) is an acute polyradiculoneuropathy marked by flaccid areflexic paralysis. Although the pathogenesis of GBS remains incompletely defined, considered as an autoimmune disease most frequently triggered by an previous infection. Antecedent infections with Campylobacter jejuni, cytomegalovirus, Ebstein-Barr virus, Mycoplasma pneumoniae, Haemophilus influenzae, human immunodeficiency virus, enterovirus, rotavirus are common. But, it is rare that GBS following typhoid fever. We present a case of typical GBS after antecedent Salmonella typhi infection.

• 미생물학회지
• /
• 제54권1호
• /
• pp.1-8
• /
• 2018

원핵 세포는 핵양체 결합 단백질(NAP)로 알려진 다양한 히스톤 유사 단백질을 가지고 있다. 이들은 DNA의 AT-rich 서열에 결합하여, DNA 자체를 감싸거나, 구부리거나, 떨어져 있는 DNA 가닥을 연결시키는 다리 역할을 하여, 결국에는 원핵 생물의 유전자 발현을 조절한다. NAP는 특히 전사의 억제 기능을 가지고 있기 때문에, 유전자 발현 억제에 있어서 이들의 역할과, 구체적인 메커니즘을 밝히는 것을 매우 중요한 일이다. 본 논문에서는 잘 알려져 있는 NAP인 H-NS와 HU에 대하여 정리하였고, 특히 E. coli와 Salmonella Typhimurium에서 이들의 유전자 발현에 대한 기능을 요약하였다. H-NS는 이들의 올리고머화와 필라멘트 구조 형성을 통하여 Salmonella와 같은 사람에 감염하는 병원성 세균의 독성유전자 발현을 억제할 수 있고, 이런 기능을 수행하였을 때 다른 NAP와 함께 작용할 수 있다. 최근에 H-NS는 사람에게 typhoid fever와 systemic disease를 발생시키는 독성물질인, typhoid toxin의 발현 또한 조절할 수 있음이 밝혀졌다. Salmonella에서 HU 또한 독성 유전자뿐만 아니라, 이들의 생리적 기능에 중요한 유전자들의 발현을 조절할 수 있다. 따라서, H-NS와 HU와 같은 NAP들이 원핵 생물의 독성 유전자 발현의 분자적인 메커니즘을 밝히는데 중요한 요소임을 제시한다.

• 대한핵의학회지
• /
• 제6권1호
• /
• pp.25-32
• /
• 1972

The plasma HGH concentrations were assayed in total 138 cases by the radioimmunoassay. The groups of control, typhoid fever, epidemic hemorrhagic fever, tuberculous meningitis and other febrile diseases were studied, also were the groups of hyperthyroidism, acromegaly and hypopitutarism. Insulin stimulation test was performed in control, typhoid fever and hypopituitarism. In the control group, the plasma HGH concentration in fasting (early morning) was $2.06{\pm}1.183m{\mu}g/ml$ and its upper limit was $4.5m{\mu}g/ml$. No sexual difference was observed. By the insulin stimulation, plasma HGH concentration had rised to the peak level of $24.1{\pm}15.71m{\mu}g/ml$, 60 min. after the intravenous insulin injection, then decreased to the normal level progressively. In typhoid fever, fasting HGH concentrations in febrile state and in defeverence were $2.5{\pm}1.35m{\mu}g/ml\;and\;2.2{\pm}3.32m{\mu}g/ml$ respectively, showing no significant difference with the control group. However, the levels of individual cases ranged widely, conpared with the control group. The response to the insulin stimulation test was similar to the control group. In epidemic hemorrhagic fever the HGH concentrations in oliguric phase, in diuretic phase and in convalescence were $4.2{\pm}3.71m{\mu}g/ml,\;2.2{\pm}1.30m{\mu}g/ml\;and\;3.4{\pm}3.01m{\mu}g/ml$ respectively. No significant differences were observe compared to the control, but they showed wide range of plasma HGH levels. In tuberculous meningitis, the fasting HGH concentration was $2.9{\pm}1.42m{\mu}g/ml$. In the other febrile diseases, the value was $2.5{\pm}2.23m{\mu}g/ml$. In 4 cases of hypopituitarism, the fasting HGH concentration was $2.3{\pm}0.42m{\mu}g/ml$ and ranged normally. However, the response to the insulin stimulation test was not observed. Very high plasma HGH concentrations were observed in acromegalic patients.

• 대한간호
• /
• 제13권3호통권71호
• /
• pp.59-68
• /
• 1974

Although many communicable diseases have been treated successfully since the advent of the antibiotic era and explosive epidemics have become less frequent in Korea as a result of continuous efforts to prevent the communicable diseases, the incidence of s

• 보건행정학회지
• /
• 제5권1호
• /
• pp.59-79
• /
• 1995

This study intended to grasp the results of performance of school health policy and to provide information for the future policy performance in Korea. As the objects of the study we chose the secondary school(142 middle schools, 111 high schools) in Pusan, 1993. The contents of the study were about school health service and school health education. For the study we collected datum and took statistics through the existing theses, books, various statistics, and interviews with the persons concerned. Therefore the method of the study was basically emperical and demonstrative. The results of the study can be presented as follows : 1. the results of performance of health service proved to be 5.40% for vaccination against hepatitis B, 5.26% for typhoid fever, 6.65% for EH fever, and 9.84% for influenza in middle schools, In high schools' 5.76% for vaccination against hepatitis B, 0.03% for typhoid fever, 0.25 for EH fever, and 0.86% for influenza. 2. The results of policy performance of health education were found out 64.08% in middle schools, and 17.12% in high schools. 3. The factors which affected the results of performance of health service turned out 1) the improper standard of policy performace that applicants should pay their own expenses. 2) the lack of performer's will to carry out the policy owing to the improper standard. 3) the shortage of interorganizational enforcement activities and communications which influence the will of performance. 4. The affecting factors to the will of performance of health education proved to be 1) the improper standard that nurse-teachers can teach students in the classroom in case of need. 2) the lack of nurse-teachers due to the above inappropriate standard. 3) the lack of enforcement activities of supervising organs which employ and direct nurse-teachers. 4) the lack of activities of organs which should communicate one another and allot nurse-teachers of public schools without fail.

• Pediatric Infection and Vaccine
• /
• 제4권1호
• /
• pp.155-159
• /
• 1997

We had experienced a case of pericarditis associated with Mycoplasma and Salmonella co-infection. This 5-year old boy presented with high fever and generalized tonic-clonic seizure. The diagnosis was based on the markedly increased serial titer of Widal test and cold agglutinin with Mycoplasma indirect hemmagglution test during hospitalization. The electrocardiogram revealed P-R interval prolongation and an echocardiogram revealed moderate pericardial effusion. The patient treated with ceftriaxone and erythromycin for 2 weeks, and responded to the treatment well. We report this case with brief review of the related literatures.

• Journal of Preventive Medicine and Public Health
• /
• 제10권1호
• /
• pp.86-93
• /
• 1977

부산시 남구 문현동 고지대 일원에서 유행한 장티푸스의 전염원과 감염경로를 규명하고 그 관리사업을 1975년 4월 28일부터 동년 6월 3일까지 실시하여 얻은 결과는 다음과 같다. 1. 원인불명의 고열질환은 장티푸스로 동정되었으며 주증상은 복통, 설사, 간장비대, 오심 구토, 비장비대장미진, 회맹부압통등이었다. 2. 평균이병기간은 25.2일이었고 발생율은 1000명당 35.6였다. 3. 장티푸스유행의 전염원은 행려환자로 추정되었다. 4. 발병으로 부터 신고까지의 기간은 $10{\sim}19$일사이가 제일 많았다. 5. 1차 대변배양검사 양성은 38.9%였고 2차 검사성적은 5.6%였다. 6. 연령별 발생분포는 $5{\sim}9$세군이 30.5%로 가장 높았으며 남녀 발생비는 남자가 여자보다 약간 높았다. 7, 교육정도는 국민학교 이하가 66.6%, 생활정도로는 저소득층이 77.8%로 각각 최고율을 점하고 있다. 8. 발병후 의료시설의 이용은 약국이 제일 높고 다음이 한약방, 의원의 순서였다.

• 대한미생물학회지
• /
• 제18권1호
• /
• pp.73-85
• /
• 1983

The advantages of the enzyme-linked immunosorbent assay(ELISA) are its sensitivity and its simplicity in detecting IgM and IgG antibodies. For applying the ELISA to the diagnosis of typhoid fever, first of all, experiments were performed to determine which concentration of killed whole cell antigens and lipopolysaccharide(LPS) antigens of S. typhi(0.901 w) were optimally coated to the wells of the polystyrene and polyvinylchloride microplate, using the hyperimmune sera from rabbits against S. typhi. By using both kinds of antigens of S. typhi adsorbed to the ELISA microplate, the changing patterns of IgG and IgM antibodies in the sera from rabbits responding to the killed whole cell antigens of S. typhi(0901 w) during the prolonged immunization were serially traced by the ELISA. At the same time, the level of antibodies against S. typhi in sera fron patients with typhoid fever and from normal healthy persons were measured by the ELISA employing the killed whole cell antigens and LPS antigens as the coating antigens. The results obtained were summerized as follow: 1. The optimal concentration of the killed whole cell antigens, which were more easily adsorbed to the polystyrene plate than the polyvinylchloride plate, was $10^8cells/ml$ of carbonate buffer(pH. 9.6) on the wells of the polystyrene plate when treated at $37^{\circ}C$ for 4 hours. On the other hand, the optimal concentration of lipopolysaccharide antigens, which were adsorbed only to the polyvinylchloride plate, was $100{\mu}g/ml$ of carbonate buffer(pH. 9.6) on the wells of the polyvinylchloride plate when treated at $37^{\circ}C$ for 4 hours. 2. IgM antibody response were dominating in rabbits responding to the killed whole cell antigens of S. typhi(0.901 w), and were more specific to the LPS antigens than to the killed whole cell antigens in the ELISA. Good correlations were made between the IgM titers by the ELISA and the aggglutinating titers of sera from the immunized rabbits. 3. Both IgG and IgM agglutination titers by the ELISA in sera from most of patients with typhoid fever were above 1:320 but those in sera from most of normal, healthy persons were below 1:80. 4. There were close correlations between the antibody titers by the ELISA and the agglutinating titers to the killed whole cell antigens in the tested human sera, IgM titers being more correlated with the agglutinating titers than IgG titers. But a little correlations were made between the antibody titers by the ELISA and the agglutinating titers to the LPS antigens. 5. IgM titers in the tested human sera were similar to IgG titers detected by the ELISA employing the killd whole cells antigens and the LPS antigens. 6. Good correlations were made between the antibody titers demonstrated by the ELISA performed on the killed whole cell antigens and the LPS antigens as the different, coating antigens on the ELISA microplates.

• Journal of Yeungnam Medical Science
• /
• 제12권2호
• /
• pp.191-202
• /
• 1995

목 적: 소아의 급성 열성 질환은 그 원인이 위장관 계통이 아니더라도 흔히 구토, 설사, 복통 등의 복부증상을 동반하나 발열의 원인이 불명이면서 복부 증상이 동반될 때는 발열의 원인이 복부장기 이상소견으로 인한 것인지, 복부장기의 이상소견으로 그 원인을 유추할 수 있는지 당연히 관심을 가지게 된다. 저자들은 발열과 복부 증상을 주소로 하는 환아에서 복부 초음파 검사를 실시하여 그의 진단적 의의에 대해 알아보기 위하여 본 연구를 시행하였다. 방 법: 1994년 1월부터 1995년 6월까지 발열과 복부증상을 주소로 울산 동강병원 소아과에 입원, 치료한 환아 중 진단적 목적의 일부분으로 복부 초음파검사를 시행한 60례를 대상으로 그 검사의 진단적 가치에 대해서 후향적으로 조사하였다. 결 과: 1) 검사 환아중 56례 (93.3%)에서 복부 초음파의 이상 소견을 보였다. 2) 복부 초음파검사의 이상 소견으로 장간막 임파선 종대를 동반하는 경우가 52례 (86.7%)로서 가장 많았고, 소량의 복수만 있었던 경우가 1례 (1.7%), 방광벽 비후가 2례 (3.5%), 미세한 대장벽 비후만 있었던 경우가 1례 (1.7%) 있었다. 3) 소량의 복수 동반 여부와 관계없이 장간막 임파선 종대만 있었던 예는 30례 (50.0%)로서 다양한 진단명으로 볼때 특별한 진단적 가치가 없었다. 4) 소량의 복수 동반 여부와 관계없이 장간막 임파선 종대와 회맹장벽 비후가 있었던 경우가 8례 (13.3%)로서 역시 특별한 진단적 가치가 없었다. 5) 회맹장벽의 비후와 관계없이 장간막 임파선 종대와 비장비후가 있었던 7례 (11.7%)에선 4례 (6.7%)에서 장티푸스로 진단되었다. 6) 장티푸스로 진단된 6례 (10.0%)의 복부 초음파소견은 장간막 임파선 종대가 6례 (100.0%) 모두에서, 비종대가 4례 (66.7%)에서 회맹장벽비후가 1례에서 (16.7%) 보였고, 장간막임파선종대와 비장비대를 같이 보인 경우가 4례 (66.7%), 장간막임파선종대, 간비장비대, 회맹장벽비후를 같이 보인 경우가 1례 (16.7%) 있었다. 7) 장간막 임파선종대와 충수돌기 부위의 이상이 같이 있었던 7례 (11.7%) 모두에서 급성 충수돌기염이나 그의 합병증으로 진단되었다. 8) 방광벽 비후가 있었던 2례 (3.3%)에서 급성 방광염과 급성 출혈성방광염으로 진단되었다. 9) 미세한 대장벽의 비후가 있었던 1례 (1.7%)에서는 이질로 진단되었다. 결 론: 발열과 복부증상을 주소로 하는 환아에서 복부 초음파검사상 회맹장벽의 비후, 장간막 임파선 종대, 비장비후 등의 소견이 있을때에는 장티푸스의 가능성을 고려해야겠으며 소아에서 복부이학적검사를 쉽게 할 수 없는 경우나 그 진단이 분명치 못한 경우 복부 초음파검사를 시행하여 장간막 임파선 종대와 충수돌기 주변 부위의 이상 소견으로 수술이 필요한 급성 충수돌기염이나 그의 합병증의 진단을 신속히 할 수 있으리라 사료된다.

• 한국미생물·생명공학회지
• /
• 제41권1호
• /
• pp.128-134
• /
• 2013

Salmonella enterica serovar typhi, a Gram-negative food-borne pathogen, causes typhoid fever in humans. OmpC is an outer membrane porin of S. typhi expressed throughout the infection period. OmpC is potentially an attractive antigen for multivalent vaccines and diagnostic kit designs. In this study we combined in silico, in vitro and in vivo approaches to analyze various aspects of OmpC's antigenic properties. The conserved region, in addition to secondary and tertiary structures, and linear B cell epitopes, were predicted. A number of results obtained from in silico analyses were validated by experimental studies. OmpC was amplified, cloned and then expressed, with the recombinant protein then being purified. BALB/c mice were immunized by purified denatured OmpC. The titer of antibody was raised. Results of challenges with the pathogen revealed that the immunity is non-protective. Most of the theoretical and experimental results were in consensus. Introduced linear B cell epitopes can be employed for the design of diagnostic kits based on antigen-antibody interactions.