• Korean Journal of Agricultural Science
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• v.42 no.2
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• pp.105-109
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• 2015

Andean potato latent virus (APLV) is a phytopathogenic virus that belongs to the Group IV (+) sense ssRNA viruses of the genus Tymovirus. It mainly infects potatoes and is specified as a controlled quarantine virus in Korea. In this study, two primer sets of RT-PCR and nested PCR [set 2 ($404{\rightarrow}259bp$) and set 23 ($501{\rightarrow}349bp$)], were selected, which can rapidly and accurately diagnose APLV in quarantine sites. In addition, a modified-positive control plasmid is development, can possible verification of laboratory contamination in diagnosis of APLV detection. The PCR-base system developed in this study is expected to diagnose APLV and contribute to the plant quarantine in Korea.

• The Plant Pathology Journal
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• v.20 no.3
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• pp.220-223
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• 2004

Most plants of commercial poinsettia cultivars grown from cuttings develop mosaic and chlorotic dot symptoms on leaves. Reverse transcription-polymerase chain reaction (RT-PCR) test showed that they were infected with Poinsettia mosaic virus (PnMV). In a survey of commercially grown poinsettias conducted in Korea, PnMV was detected in ten of ten poinsettia cultivars sampled and in 100％ of 178 samples tested. The virus has isometric particles and about 29 nm in diameter. Crystalline virus particles were observed in cytoplasm of cells of diseased plants by transmission electron microscopy. Nucleotide sequence of coat protein gene of PnMV- Kl showed 97.3％ homology with that of a German isolate. This is the first report on PnMV in Korea.