Nunez-Ramirez, Diola Marina;Medina-Torres, Luis;Valencia-Lopez, Jose Javier;Calderas, Fausto;Lopez-Miranda, Javier;Medrano-Roldan, Hiram;Solis-Soto, Aquiles
Journal of Microbiology and Biotechnology
/
v.22
no.11
/
pp.1494-1500
/
2012
Fermentation with filamentous fungi in a bioreactor is a complex dynamic process that is affected by flow conditions and the evolution of the rheological properties of the medium. These properties are mainly affected by the biomass concentration and the morphology of the fungus. In this work, the rheological properties of a fermentation with the fungus Beauveria bassiana under different hydrodynamic conditions were studied and the rheological behavior of this broth was simulated through a mixture of carboxymethyl cellulose sodium and cellulose fibers (CMCNa-SF). The bioreactor was a 10 L CSTR tank operated at different stir velocities. Rheological results were similar at 100 and 300 rpm for both systems. However, there was a significant increase in the viscosity accompanied by a change in the consistence index, calculated according to the power law model, for both systems at 800 rpm. The systems exhibited shear-thinning behavior at all stir velocities, which was determined with the power law model. The mixing time was observed to increase as the cellulose content in the system increased and, consequently, the efficiency of mixing diminished. These results are thought to be due to the rheological and morphological similarities of the two fungal systems. These results will help in the optimization of scale-up production of these fungi.
This work covers an important point of the benchmark released by the expert group on Uncertainty Analysis in Modeling of Light Water Reactors. This ambitious benchmark aims to determine the uncertainty in light water reactors systems and processes in all stages of calculation, with emphasis on multi-physics (coupled) and multi-scale simulations. The Gesellschaft für Anlagen und Reaktorsicherheit methodology is used to propagate the thermal-hydraulic uncertainty of macroscopic parameters through TRACE5.0p3/PARCSv3.0 coupled code. The main innovative points achieved in this work are i) a new thermal-hydraulic model is developed with a highly-accurate 3D core discretization plus an iterative process is presented to adjust the 3D bypass flow, ii) a control rod insertion occurrence -which data is obtained from a real PWR test- is used as a transient simulation, iii) two approaches are used for the propagation process: maximum response where the uncertainty and sensitivity analysis is performed for the maximum absolute response and index dependent where the uncertainty and sensitivity analysis is performed at each time step, and iv) RESTING MATLAB code is developed to automate the model generation process and, then, propagate the thermal-hydraulic uncertainty. The input uncertainty information is found in related literature or, if not found, defined based on expert judgment. This paper, first, presents the Gesellschaft für Anlagen und Reaktorsicherheit methodology to propagate the uncertainty in thermal-hydraulic macroscopic parameters and, then, shows the results when the methodology is applied to a PWR reactor.
The turbulent flow behavior of air supply and exhaustion in the Shin-gum-ho subway station is analyzed for ordinary and emergency state. The depth of Shin-gum-ho station is 43.6m which consists of the island-type platform(8th floor in underground) and a two-story lobby (first & second floor in underground). An emergency stairway connects between the platform and the lobby. Ventilation operation mode for ordinary state is set up as a combination of air supply and exhaustion in the lobby and platform, while for emergency state it is set up as a full air supply in the lobby and a full exhaustion in the platform. The entire station is covered for simulation. The ventilation diffusers are modeled as 95 square shapes of $0.6m{\times}0.6m$ in the lobby and as 222 square shapes of $0.6m{\times}0.6m$ and 4 rectangular shapes of $1.2m{\times}0.8m$ in the platform. The total of 7.5million grids are generated and whole domain is divided to 22 blocks for MPI efficiency of calculation. Large eddy simulation(LES) is applied to solve the momentum equation and Smagorinsky model($C_s$=0.2) is used as SGS(subgrid scale) model. The time-averaged velocity fields are compared to experimental data and show a good agreement with it.
Sodium-cooled fast breeder reactors use liquid sodium as a moderator and coolant to transfer heat from the reactor core. The main hazard associated with sodium is its rapid reaction with water. Sodium-water reaction (SWR) takes place when water or vapor leak into the sodium side through a crack on a heat-transfer tube in a steam generator. If the SWR continues for some time, the SWR will damage the surface of the defective area, causing it to enlarge. This self-enlargement of the crack is called "self-wastage phenomena." A stepwise numerical evaluation model of the self-wastage phenomena was devised using a computational code of multicomponent multiphase flow involving a sodium-water chemical reaction: sodiumwater reaction analysis physics of interdisciplinary multiphase flow (SERAPHIM). The temperature of gas mixture and the concentration of NaOH at the surface of the tube wall are obtained by a numerical calculation using SERAPHIM. Averaged thermophysical properties are used to assess the local wastage depth at the tube surface. By reflecting the wastage depth to the computational grid, the self-wastage phenomena are evaluated. A two-dimensional benchmark analysis of an SWAT (Sodium-Water reAction Test rig) experiment is carried out to evaluate the feasibility of the numerical model. Numerical results show that the geometry and scale of enlarged cracks show good agreement with the experimental result. Enlarged cracks appear to taper inward to a significantly smaller opening on the inside of the tube wall. The enlarged outer diameter of the crack is 4.72 mm, which shows good agreement with the experimental data (4.96 mm).
Lee, Jae Seung;Jung, Dong J.;Lee, Ho Jin;Lee, Gang Bong;Heo, Nam Hoe;Jang, Ho G.
Bulletin of the Korean Chemical Society
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v.21
no.10
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pp.969-972
/
2000
[Fe II Fe III $BPLNP(OAc)_2](BPh_4)_2$ (1), a new model for the reduced form of the purple acid phosphatases, has been synthesized by using a dinucleating ligand, 2,6-bis[((2-pyridylmethyl)(6-methyl-2-pyridylmethyl)ami-no)methyl]-4-nitrophenol (HBPLNP) . Complex 1 has been studied by electronic spectral, NMR, EPR, SQUID, and electrochemical methods. Complex 1 exhibits two strong bands at 498 nm $(\varepsilon=$ 2.6 ${\times}10^3M-^1cm-^1)$ and 1363 nm $(\varepsilon=$ 5.7 ${\times}10^2M-^1cm-^1)$ in $CH_3CN.$ These are assigned to phenolate-to-FeIII and intervalence charge-transfer transitions, respectively. NMR spectrum of complex 1 exhibits sharp isotropically shifted resonances, which number is half of those expected for a valence-trapped species, indicating that electron transfer between FeⅡ and FeⅢ centers is faster than NMR time scale at room temperature. Complex 1 undergoes quasireversible one-electron redox processes. The $FeIII_2/FeIIFeIII$ and $FeIIFeIII/FeII_2$ redox couples are at 0.807 and 0.167 V ver-sus SCE, respectively. It has Kcomp = 5.9 ${\times}$10 1s(acetato) ligand combination sta-bilizes a mixed-valence FeIIFeIII complex in the air. Interestingly, complex 1 exhibits intense EPR signals at g = 8.56, 5.45, 4.30 corresponding to mononuclear high-spin FeⅢ species, which suggest a very weak magnetic coupling between the iron centers. Magnetic susceptibility study shows that there is a very weak antiferromag-netic coupling (J = $-0.78cm-^1$, H = $-2JS_1${\times}$S_2)$ between FeII and FeIII centers. Thus, we can suggest that complex 1 has a very weak antiferromagnetic coupling between the iron centers due to the electronic effect of the nitro group in the bridging phenolate ligand.
With the assistance of typhoon field data at aerial elevation level observed by meteorological satellites and wind velocity and direction records nearby the ground gathered in Guangzhou Weather Station between 1985 and 2001, some key wind field parameters under typhoon climate in Guangzhou region were calibrated based on Monte-Carlo stochastic algorithm and Meng's typhoon numerical model. By using Peak Over Threshold method (POT) and Generalized Pareto Distribution (GPD), Wind field characteristics during typhoons for various return periods in several typical engineering fields were predicted, showing that some distribution rules in relation to gradient height of atmosphere boundary layer, power-law component of wind profile, gust factor and extreme wind velocity at 1-3s time interval are obviously different from corresponding items in Chinese wind load Codes. In order to evaluate the influence of typhoon field parameters on long-span flexible bridges, 1:100 reduced-scale wind field of type B terrain was reillustrated under typhoon and normal conditions utilizing passive turbulence generators in TJ-3 wind tunnel, and wind-induced performance tests of aero-elastic model of long-span Guangzhou Xinguang arch bridge were carried out as well. Furthermore, aerodynamic admittance function about lattice cross section in mid-span arch lib under the condition of higher turbulence intensity of typhoon field was identified via using high-frequency force-measured balance. Based on identified aerodynamic admittance expressions, Wind-induced stochastic vibration of Xinguang arch bridge under typhoon and normal climates was calculated and compared, considering structural geometrical non-linearity, stochastic wind attack angle effects, etc. Thus, the aerodynamic response characteristics under typhoon and normal conditions can be illustrated and checked, which are of satisfactory response results for different oncoming wind velocities with resemblance to those wind tunnel testing data under the two types of climate modes.
Journal of Korean Society of Occupational and Environmental Hygiene
/
v.24
no.3
/
pp.310-320
/
2014
Backgrounds: Endotoxin, which found in the outer membrane of the gram-negative bacteria cell wall, makes up almost all of the lipopolysaccharide(LPS). When people are exposed to endotoxin,it can result in diverse health effects such as an airway irritation and inflammation, fever, malaise, bronchitis, allergic asthma, toxic pneumonitis, hypersensitivity lung disease. Cases among the elderly, children or pregnant can occur more frequently than a healthy adult if they are repeatedly exposed to the existing endotoxin. Therefore, we investigated and assessed the environmental characteristics associated with the airborne endotoxin concentration level in six hospital lobbies. Method: Endotoxin from indoor air in six hospital lobbies was measured by an area sampling method and analyzed according to American Society for Testing and Materials International(ASTM international) E2144-01. Total suspended particulate(TSP), carbon dioxide($CO_2$), temperature and humidity were also measured by using direct reading measurements or airborne sampling equipment at the same time. Environmental characteristics were appropriately divided into two or three groups for a statistics analysis. One-way analysis variable(one-way ANOVA) was used to examine a difference of the endotoxin concentration, depending on the environmental characteristics. In addition, only variables with p-value(p<0.25) were eventually designed to the best model by using multiple regression analysis. Results: The correlation analysis result indicated that TSP(p=0.003) and $CO_2$(p<0.0001) levels were significantly associated with endotoxin concentration levels. In contrast, temperature(p<0.068) and humidity(p<0.365) were not associated with endotoxin concentration. Levels of endotoxin concentration were statistically different among the environmental characteristics of Service time(p=0.01), Establishment of hospital(p<0.001), Scale of hospital(p=0.01), Day average people using hospital(p=0.03), Cleaning time of lobby(p=0.05), Season(p<0.001), and Cleaning of ventilation system(p<0.001) according to ANOVA. Finally, the best model(Adjusted R-square=72%) that we designed through a multiple regression test included environmental characteristics related to Service time, Area of lobby, Season, Cleaning of ventilation system, and Temperature. Conclusions: According to this study, our result showed a normal level of endotoxin concentration in the hospital lobbies and found environmental management methods to reduce the level of endotoxin concentration to a minimum. Consequently, this study recognized to be requirement for the management of ventilation systems and an indoor temperature in order to reduce the level of endotoxin concentration in the hospital lobbies.
Journal of the Korean Society of Fisheries and Ocean Technology
/
v.35
no.2
/
pp.93-101
/
1999
A simple experimental method was used in an attempt to realize the elevation of the fishing ability of purse seine in the sea area of Cheju Island, the changes of seine volume and tension in the purseline during pursing. Experiments carried out on the six types simplified reduced model seines which were made of knotless nettings. The nettings were woven in different leg length 4.3, 5.0, 5.5, 6.0, 6.6 and 7.7mm of polyester 28 tex two threads two-ply twine, and each of the seines were named I, II, III, IV, V and Ⅵ seine. Dimension of seine models were 450cm for corkline and 85cmfor seine depth, each seines rigged up 160g of float for a floatline and 50g (underwater weight) of lead for a leadline. These model purse seines were made of the scale of 1/200 of its full scale, a 120 ton in the near sea of Cheju Island. Designing and testing for the model purse seines were based on the Tauti's law. Experiments were measured in the observation channel of a flume tank at the static conditions set up shooting and pursing equipments. Motion of purse seine during purse line was recorded by the two sets video camera for VTR which were placed in top and front of the model seine. The reading coordinate of seine volume carried out by the video digitization system, disk data for the purseline tension. An analysis were performed on the changes seine volume and tension in the purseline during pursing. The results obtained were as follows: 1. The seine volume during pursing was largest for Ⅵ seine with smallest d/l followed by V, IV, III, II and I seines, and tension in the purseline was small. 2. Seine volume during pursing can be expressed by the following equation; CVt=l-EXP[{2.79 (d/l)+0.35}t-33.37 (d/l) + 0.57] Where CVt is volume ratio, d is twine diameter, l is leg length and t is pursing time (sec). 3. Tension in the purse line during pursing can be expressed by the following equation; T= 1- EXP {0.57t + 13.36 (d/l)+2.97} Where T is tension (kg) in the purseline during pursing.
Journal of the Korean Association of Geographic Information Studies
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v.23
no.1
/
pp.15-29
/
2020
Recently, the incidence of flooding in Korea has decreased by the measures by central and local governments, however the scale of damage is increasing due to the improvement of living standard. One of the causes of such flood damage is natural causes such as rainfall exceeding the planned frequency of flood control under climate change. In addition, there are artificial causes such as encroachment of river spaces and management problems in upstream basins without consideration of downstream damage potential by regional development flood. In this study, in order to reduce the inundation damage caused by flooding of river, the situation at the time of inundation damage was reproduced by the detailed topographic data and 2D numerical model. Therefore, the effect of preparing various disaster prevention measures for the lowland was simulated in advance so that quantitative evaluation could be achieved. The target area is Taehwa river basin, where flooding was caused by the flooding of river waters caused by typhoon Chaba in October 2016. As a result of rainfall-discharge and two-dimensional analysis, the simulation results agree with the observed in terms of flood depth, flood arrival time and flooded area. This study examined the applicability of hydraulic analysis on river using two-dimensional inundation model, by applying detailed topographic data and it is expected to contribute to establish of disaster prevention measures.
Journal of the Korean Society of Marine Environment & Safety
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v.27
no.7
/
pp.1004-1010
/
2021
Ship-floating object accidents can lead not only to a delay in ship's operations, but also to large scale casualties. Hence, preventive measures are required to avoid them. This study analyzed the spatiotemporal aspects of such collisions based on the data on ship-floating object accidents in sea areas in the last five years, including the collisions in South Korea's territorial seas and exclusive economic zones. We also provide basic data for related research fields. To understand the distribution of the relative density of accidents involving floating objects, the sea area under analysis was visualized as a grid and a two-dimensional histogram was generated. A multinomial logistic regression model was used to analyze the effect of variables such as time of day and season on the collisions. The spatial analysis revealed that the collision density was highest for the areas extending from Geoje Island to Tongyeong, including Jinhae Bay, and that it was high near Jeongok Port in the West Sea and the northern part of Jeju Island. The temporal analysis revealed that the collisions occurred most frequently during the day (71.4%) and in autumn. Furthermore, the likelihood of collision with floating objects was much higher for professional fishing vessels, leisure vessels, and recreational fishing vessels than for cargo vessels during the day and in autumn. The results of this analysis can be used as primary data for the arrangement of Coast Guard vessels, rigid enforcement of regulations, removal of floating objects, and preparation of countermeasures involving preliminary removal of floating objects to prevent accidents by time and season.
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