• Title/Summary/Keyword: Two-stage expansion

검색결과 144건 처리시간 0.035초

Effect of artificial shrinkage on clinical outcome in fresh blastocyst transfer cycles

  • Hur, Yong-Soo;Park, Jeong-Hyun;Ryu, Eun-Kyung;Yoon, Hae-Jin;Yoon, San-Hyun;Hur, Chang-Young;Lee, Won-Don;Lim, Jin-Ho
    • Clinical and Experimental Reproductive Medicine
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    • 제38권2호
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    • pp.87-92
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    • 2011
  • Objective: This study aimed to determine the safety and clinical effect of artificial shrinkage (AS) in terms of assisted hatching of fresh blastocysts. Also, we evaluated the correlation between patient age and the effect of AS on clinical outcome. Methods: Two AS methods, using a 29-gauge needle and laser pulse, were compared. Seventy-three blastocysts were shrunk using a 29-gauge needle and the same number of other blastocysts were shrunk by a laser pulse. We evaluated the shrunken blastocysts hourly and considered them viable if they re-expanded >70%. Blastocyst transfer cycles (n=134) were divided into two groups: a control group consisted of the cycles whose intact embryos were transferred (n=100), while the AS group consisted of the cycles whose embryos were replaced following AS (n=34). The implantation and pregnancy rates of the control group and AS group were compared ($p$ <0.05). Results: The re-expansion rates of the 29-gauge needle and laser pulse AS groups were similar (56 [76.7%] vs. 62 [84.9%], respectively). All of the remaining shrunken blastocysts were re-expanded within 2 hours. There was no degeneration of shrunken blastocysts. The total and clinical pregnancy rate of the AS group (23 [67.6%]; 20 [58.8%], respectively) was significantly higher than that of the control group (47 [47.0%]; 39 [39.0%], respectively). In the older patient group, there was no difference in the clinical outcomes between the AS and control groups. Conclusion: These results suggest that AS of blastocoele cavity, followed by the transfer, would be a useful approach to improve the clinical outcome in cycles in which fresh blastocyst stage embryos are transferred.

체외성숙, 수정 및 체외배양에서 생산된 소 배반포기배의 ICM과 Trophectoderm세포수에 관한 연구 (ICM-Trophectoderm Cell Numbers of Bovine IVM/IVF/IVC Blastocysts)

  • 김은영;엄상준;김선의;윤산현;박세필;정길생;임진호
    • 한국가축번식학회지
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    • 제20권1호
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    • pp.27-34
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    • 1996
  • 본 연구는 immunosurgery와 polynucleotide-specific 형광물질을 이용한 differential labelling기법으로 체외에서 소 수정란의 배발달을 유기하는데 효과적인 것으로 알려진 CR1배양액을 사용하여 체외생산된 소 배반포기배의 inner cell mass (ICM)와 trophectoderm(TE)의 총 세포수를 조사하고자 실시하였다. 공시 배반포기배는 체외수정 후 8일째에 얻어졌다. 체외생산된 배반포기배는 배반포강의 확대와 투명대 두께의 감소를 기준으로 초기, 중기 및 팽윤단계로 구분하였으며, 또한 같은 배발달군내의 배반포기배는 다시 두 군으로 나누어 bisbenzimide만을 처리하여 얻어진 총세포수와 immunosurgery와 two polynulceotide-specific 형광물질을 이용하여 얻어진 ICM와 TE의 총세포수를 비교하여 얻어진 결과는 다음과 같다. 1) 체외수정 후 8일째 배반포기배의 발달율은 29.3%였으며, 초기, 중기, 팽윤 및 부화단계로 구분하였을 때의 발달율은 각각 8.7, 9.9, 7.6, 3.1%였다. 2) Bisbenzimide를 이용한 배반포기배의 총 세포수는 초기, 중기 및 팽윤단게가 각각 46.9$\pm$8.6, 66.2$\pm$12.5, 122.8$\pm$14.4를 나타냈다. 이러한 결과는 CR1이 소 수정란의 발달에 적절한 배양액임을 알 수 있었다. 3) Immunosurgery와 polynucleotide-specific 형광물질을 이용한 differential labelling기법으로 배반포기배의 ICM과 TE 세포수를 초기, 중기 및 팽윤단계로 나누어 조사한 결과, ICM 세포수는 각각 12.8$\pm$5.9, 26.3$\pm$8.4, 35.5$\pm$15.0개 이었고, TE 세포수는 각각 30.5$\pm$5.0, 41.3$\pm$8.2, 81.1$\pm$13.4개로 나타나 ICM과 TE 세포수는 초기 배반포기배에서 팽윤 배반포기배로 진행됨에 따라 두배에서 세배 정도 증가되었음을 알 수 있었다. 또한, differential labelling과 bisbenzimide기법에서 얻어진 각각의 총세포수를 비교하였을 때 총세포수는 발달의 진행 정도에 따라 증가되며 그와 동시에 동일한 군 간의 세포수도 거의 유사함을 알 수 있었다. 따라서, ICM과 TE를 differential labelling하는 기법은 수정란의 quality를 평가하는데 매우 유용한 기법으로서 착상전 embryo 발달을 연구하는데 효과적으로 이용될 수 있다는 것을 시사한다.

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농촌지역 수출 시행단계 농가의 수출 경험에 관한 질적 연구 (Qualitative Research on the Rural Farmers' Exporting Experience in Stage of Conducting Export)

  • 이향미;고종태
    • 농촌계획
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    • 제21권3호
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    • pp.155-169
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    • 2015
  • As an essential means to the exploitation of new demands, the export of agri-food has the price-supporting effect on the domestic agri-food and its expansion can be a good way to increase farms' income. Of course, to enhance farms' income under the liberalization of the market, it is necessary to have countermeasures considering various factors. To this end, the study understood the experiences of farms doing exports and looked into ways to improve them by reviewing their motivations to export, difficulties, requirements, etc. In this context, the study conducted the purposive sampling among tomato export farmhouses in Jeongseon-gun in the export enforcement phase and executed the theme analysis. The research findings and suggestions are as follows. First, in order to enhance the level of commercialization of Jeongseon-gun tomatoes and reinforce the trade bargaining power, it is necessary to form the consensus on policy and make the scaling of the site based on the strong organization power. To this end, it requires the active role of agricultural cooperatives that take a big portion of distribution in the site and distribute its profits directly to farmhouses. Second, it is necessary to set clear target markets for Jeongseon-gun tomato export farms and set sales strategies for each target market. That is, in order to expand the export of Jeongseon-gun tomatoes, after clarifying local sales and export sales, export farmhouses should focus on quality management and secure the quantity for export. Third, to expand the export to Japan which already has the foundation for the export of Korean tomatoes, and to new export countries of China, Russia, etc., it is necessary to set the 'Two-Track Strategies for Export Market (particular item for focus and particular area for focus)' through the building of network of tomato export areas in Gangwon-do. In conclusion, to expand the export of Jeongseon-gun tomatoes, agricultural cooperatives and leaders together should build the strong organizational power with farmhouses and secure the quantities for export. And, according to the 'Two-Track Strategies for Export Market (particular item for focus and particular area for focus)', export infrastructure and export operational system should be built and to do so, the solidarity of tomato export farmhouses in Gangwon area should precede.

역할 창조를 위한 '몸틀(body schema)' 형성 연구 (A Study on Forming 'Body Schema' for Role Creating)

  • 송효숙
    • 한국연극학
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    • 제52호
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    • pp.319-357
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    • 2014
  • Formation of 'body schema' is the start for actor to create role and becomes the root and the foundation of existing as a role on the stage. For this, an actor needs to form 'scheme of role' with escaping from own 'body schema.' 'Schema of role' is formed by acquiring through synthesizing daily basic actions, namely, walking, standing, sitting, hand stretching, bending, and touching. The body schema, which was made with simple and usual actions, has fundamental significance in a sense of becoming the body in which the past traces in a role are habituated while energy as a role flows. As for the process of forming body schema, an actor first needs to obtain the visualized materials like photo, magazine, picture and image available for seeing a role specifically and clearly based on what analyzed a character. An actor needs to have three-dimensional image available for always recalling it in the head during acting. To do this, image data available for fundamentally capturing routine actions along with body structure are still more useful. Next, the body schema is formed by interaction with environment. Thus, there is a need of passing through the two-time process of forming body schema. Firstly, the body schema is made on routine actions in a role as physical condition of a role in actor's own everyday life. Secondly, the body schema is made on routine actions available for moving efficiently and economically in line with the environment of performance. A theatrical stage is the temporal space of rhythm and rule different from routine space. What forms body schema immediately in the second phase without body schema in the first phase ultimately becomes what exists as actor's own body, not the body of a role. The body schema, which was formed as the second process, is what truly has identity as a role in the ontological aspect, comes to experience the oppositional force in muscle, a qualitative change in energy, and emotional agitation in the physical aspect, and experiences perception, thinking, volition, and even consciousness with the entire body in the cognitive dimension. Thus, the formation of body schema can be known to be just a method of changing even spiritual and emotional layer. Body schema cannot be made if there is no process of embodiment and habit. Embodiment and habit are not simply the repeated, empty and mechanical action in the body. But, habit itself has very important meanings for forming body schema for role creating. First, habit allows the body itself to learn and understand a meaning. Second, habit relies upon environment, thereby allowing an actor of making the habituated body schema to recognize environment. Third, habit makes the mind. The habituated body schema is just the mind and the ego of a person who possesses the body schema. Fourth, habit comes to experience the expansion in energy and the expansion in existence. It may be experienced through interrelation among actor's body, tool, and environment. Fifth, habit makes identity of the body. Hence, this just becomes what secures identity of a role. These implications of habit are the formation of body schema, which is maintained with the body of being remembered firmly through being closely connected with the process of neural adaptation. Finally, it sought for possibility of practice as one method of forming body schema for role creating through Deleuze's '-becoming' theory. As 'actual animal-becoming' is real '-becoming' of forming structural transformation in the physical dimension, it meets with what the formation of body schema pursues actuality and reality. This was explained with a concept as saying of 'all '-becoming' molecular' by Deleuze/Guattari. 'Animal of having imitated animal's characteristic- becoming' is formed by which the body schema relies upon environment. In this way, relationship among the body, tool and environment has influence even upon a change in consciousness, thinking, and emotion, thereby being able to be useful for forming body schema in a sense of possibly experiencing ultimately expansion in role, namely, expansion in existence.

DMZ 세계평화공원 조성을 위한 국제공조 방안: NGO의 국제연대 활동과 지원을 중심으로 (International Cooperation Plan for DMZ World Peace Park: Focusing on NGO's international solidarity and support)

  • 서경도
    • 디지털융복합연구
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    • 제14권12호
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    • pp.645-655
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    • 2016
  • 본 연구의 목적은 'DMZ 세계평화공원' 조성에서 NGO국제연대의 역할과 기능, 그리고 작동의 메커니즘, NGO국제연대의 정책형성 등에 대하여 분석하고자 한다. DMZ 세계평화공원 조성은 북한과 대한민국 정부의 합의를 기초로 하여 양 주체간의 대화노력의 결과로 얻어질 수 있는 문제지만 그러한 결과를 실현하기 위한 과정상의 문제에 있어서 보다 더 완화된 접근 방법을 강구하여야 하며, 장기적으로 단계적인 계획 하에 이루어져야 한다. 이를 실현하는 과정에서 남한 정부의 독자적인 노력만으로는 어려우며, 국제사회와 더불어 북한이 대화협상의 의지를 가지도록 다양한 대화채널을 모색하여야 하며, 이는 곧 북한의 접근 경로 상 제3의 영역인 NGO의 역할에 주목하여야 한다. 결과적으로 'DMZ 세계평화공원' 조성하기 위해서는 DMZ 세계평화공원의 구체적인 실천 로드맵의 설정이 필요한데, 1단계로는 대통령 또는 국무총리 직속의 'DMZ 세계평화공원 추진위원회'의 구성을 하고 운영하는 것, 후보지의 선정, 북한의 호응과 국제사회의 지지에 대한 호소, 민간의 자발적 참여가 필요하다. 2단계는 소재지의 최종확정이 필요하며, 3단계로는 평화공원의 조속한 착공, DMZ평화적 이용의 확대, DMZ 접경지역의 생태 역사 문화관광의 확대 등의 단계별 로드맵이 마련될 필요가 있다.

Efficient simulation method for a gas inflow to the central molecular zone

  • Shin, Jihye;Kim, Sungsoo S.;Baba, Junichi;Saitoh, Takayuki R.;Chun, Kyungwon;Hozumi, Shunsuke
    • 천문학회보
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    • 제40권1호
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    • pp.59.1-59.1
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    • 2015
  • We present hydrodynamic simulations of gas clouds that inflowing from the disk to a few hundred parsec region of the Milky Way. Realistic Galactic structures are included in our simulations by thousands of multipole expansions that describe 6.4 million stellar particles of a self-consistent Galaxy simulation (Baba, Saitoh & Wada, in prep.). We find that a hybrid multipole expansion model with two different basis sets and a thick disk correction well reproduces the overall structures of the Milky Way. We find that the nuclear ring evolves into 240 pc at T~1500 Myr, regardless of the initial size. For most of simulation runs, gas inflow rate to the nuclear region is equilibrated as ~0.02 Msun/yr, and thus accumulated gas mass and star formation activity is stabilized as $6{\times}10^7Msun$ and ~0.02M/yr, respectively. These stabilized values are in a good agreement with estimations for the CMZ. The nuclear ring is off-centered to the Galactic center by the lopsided central mass distribution of the Galaxy model, and thus an asymmetric mass distribution is arose accordingly. The lopsidedness also leads the nuclear ring to be tilted to the Galactic plane and to precess along the Galaxy rotation. In early evolutionary stage when gas clouds start to inflow and form the nuclear ring, the z-directional oscillations of the gas clouds results in the twisted, infinity-shaped nuclear ring. Since the infinity-shaped feature is transient only for first 100 Myr, the current infinity-shape observed in the CMZ may indicate that the CMZ forms quite recently.

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Effects of Water Stress by PEG on Growth and Physiological Traits in Rice Seedlings

  • Choi, Weon-Young;Kang, Si-Yong;Park, Hong-Kyu;Kim, Sang-Su;Lee, Ki-Sang;Lee, Kyu-Seong;Shin, Hyun-Tak;Choi, Sun-Young
    • 한국작물학회지
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    • 제45권2호
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    • pp.112-117
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    • 2000
  • This study was conducted to evaluate the drought tolerance of Japonica and Indica rice cultivars during germinating and seedling stages by using the polyethylene glycol (PEG) solution. Each 5 cultivars of Japonica and Indica were cultured from 14 days after seeding(DAS) to 21 DAS using the PEG solution in a moderate water potential (-0.63 MPa). The lengths of radicle and plumule during the germinating stage were inhibitied by the PEG treatment to about 50% and 85%, respectively. The application of PEG to the seedling of two rice types caused to inhibit the plant height and leaf age about 23 % and 10%, respectively. Shoot and root dry weights by PEG treatment were inhibited more severely in Japonica than those in Indica. The difference on delaying of leaf area expansion between both rice types was not found with treatment of PEG, while the leaf color was increased in both Japonica and Indica by 19.9% and 9.2%, respectively. The average photosynthetic ability was inhibited more in Japonica to 36.0% than did Indica to 27.9%. The stomatal conductance was severely affected by PEG treatment, and the degree was varied in both rice types, ranged with 80-85% in Japonica and 29.3-81.6% in Indica. These results indicate there is little relationship between seed germination and seedling growth under the stress of low water potential.

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A Simulation Method For Virtual Situations Through Seamless Integration Of Independent Events Via Autonomous And Independent Agents

  • Park, Jong Hee;Choi, Jun Seong
    • International Journal of Contents
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    • 제14권3호
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    • pp.7-16
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    • 2018
  • The extent and depth of the event plan determines the scope of pedagogical experience in situations and consequently the quality of immersive learning based on our simulated world. In contrast to planning in conventional narrative-based systems mainly pursuing dramatic interests, planning in virtual world-based pedagogical systems strive to provide realistic experiences in immersed situations. Instead of story plot comprising predetermined situations, our inter-event planning method aims at simulating diverse situations that each involve multiple events coupled via their associated agents' conditions and meaningful associations between events occurring in a background world. The specific techniques to realize our planning method include, two-phase planning based on inter-event search and intra-event decomposition (down to the animated action level); autonomous and independent agents to behave proactively with their own belief and planning capability; full-blown background world to be used as the comprehensive stage for all events to occur in; coupling events via realistic association types including deontic associations as well as conventional causality; separation of agents from event roles; temporal scheduling; and parallel and concurrent event progression mechanism. Combining all these techniques, diverse exogenous events can be derived and seamlessly (i.e., semantically meaningfully) integrated with the original event to form a wide scope of situations providing chances of abundant pedagogical experiences. For effective implementation of plan execution, we devise an execution scheme based on multiple priority queues, particularly to realize concurrent progression of many simultaneous events to simulate its corresponding reality. Specific execution mechanisms include modeling an action in terms of its component motions, adjustability of priority for agent across different events, and concurrent and parallel execution method for multiple actions and its expansion for multiple events.

사내근로복지기금제도를 통한 이윤공유참여의 생산성효과 (The Effects of Profit-Sharing Schemes on Productivity through Firm's Contribution to the Employee Welfare Fund)

  • 신범철
    • 노동경제논집
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    • 제26권3호
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    • pp.115-147
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    • 2003
  • 이 논문은 사내근로복지기금제도를 통한 이윤공유참여가 노동생산성에 미치는 효과를 한국의 상장 및 비상장 기업을 대상으로 실증 분석하였다. 이러한 분석을 위해 1992년에서 2000년까지의 한국신용평가사에서 발행한 모든 기업에 대한 재무제표 자료와 노동부의 사내근로복지기금 자료를 결합하여 패널자료를 구축하였고, 설정된 생산함수모형을 2단계 최소자승법과 패널기법에 의해 추정 분석하였다. 이 논문의 추정 결과에 의하면 사내근로복지기금에 의한 이윤공유참여가 자본의 체화효과를 통해서 그리고 노사관계 개선이나 조직의 효율성 증대와 같은 비체화효과를 통해 생산성을 향상시키는 것으로 나타났다. 이 결과는 이윤공유참여제도가 생산성에 부정적인 영향을 미친다는 대리인비용이론의 주장과는 반대의 결과이며, 한국 고유의 이윤참여제도로서 사내근로복지기금제도의 활성화를 위해 정부의 세제 및 금융상의 지원이 확대될 필요가 있음을 시사하고 있다.

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Site suitability for conifer plantation and a new challenge to utilize deciduous trees

  • NAGASHIMA, Keiko
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 추계학술대회
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    • pp.24-24
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    • 2018
  • Degraded plantation forests are increasing because of unfavorable forestry conditions prevailing in Japan, including falling timber prices, increasing operational costs, and aging and declining forestry workforce. To remedy this situation, appropriate management strategy is required. This study introduces the challenges of Odai Town, Mie Prefecture that employed a new management strategy by evaluating site suitability for conifer forests and that proposes a new forest management regime of planting deciduous trees in unsuitable sites. The site suitability for conifer forests was evaluated from two aspects: the natural site conditions and the relationship among site conditions, growth, and damage by Anaglyptus subfasciatus Pic. in Cryptomeria japonica D. Don and Chamaecyparis obtusa Sieb. Et Zucc. forests. By analyzing the relationship among site conditions, growth, and insect damage based on field data obtained in plantation forests, growth evaluation and insect damage evaluation maps were developed. Based on the natural forest investigation, natural site condition maps for C. japonica and C. obtusa were established. Furthermore, by integrating these evaluation maps with the forest road maps showing the accessibility to the forest, the forest management regime for the whole plantation area of Odai Town was established. The forest management regime map indicates the sites suitable for forestry: suitable for long-rotation, short-rotation, and potential sites for short-rotation. The sites unsuitable for forestry were considered to be more suitable for broadleaved forests. Clear-cutting was conducted in a small area and different seral stage saplings (approximately 20 deciduous tree species) suitable to the site conditions were planted in an area of $80-120m^2$ protected by deer-fences. This might establish a forest composed of many species with a multilayer vertical forest structure in a short period. The planted saplings were distributed neither randomly nor uniformly to reflect the natural distribution of trees in the forest. A challenge to develop new products using the deciduous trees has started, such as wood chips for preparing smoked food, essential oil, and deodorizer. As these challenges have just begun, their effects on enhancing sustainable resource management are still being monitored. Even with the challenges, this regime can be of high value as a management strategy to remedy the situation of expansion of degraded forests in Japan.

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