• Title/Summary/Keyword: Two-flow nozzle

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Evaluation of Sperm Sex-Sorting Method using Flow Cytometry in Hanwoo (Korean Native Cattle)

  • Yoo, Han-Jun;Lee, Kyung-Jin;Lee, Yong-Seung;Lee, Chang-Woo;Park, Joung-Jun;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • Journal of Embryo Transfer
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    • v.27 no.1
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    • pp.37-43
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    • 2012
  • This study evaluated a method of sorting X and Y chromosomes based on size using the forward angle light scatter related refractive index (FSC) of a flow cytometer. Hanwoo bulls sperm were separated to X and Y chromosomes by the parameters of FSC or Hoechst 33342 intensity. As a result, using monitor program linked flow cytometry during sorting processing, the purities were $97{\pm}0.57$ or $96{\pm}0.67%$ for the X-fraction and $96{\pm}0.33$ or $97{\pm}1.33%$ for the Y-fraction in the two sperm sorting methods. There were no differences in the X and Y ratios (X and Y %) between the sperm sorting methods based on FSC or DNA content. The proportions of female and male embryos used for in vitro fertilization and development were $66.03{\pm}3.31$ or $69.37{\pm}1.41%$, and $70.56{\pm}2.42$ or $56.11{\pm}3.09%$ when sperm were processed using the sex sorting method by FSC or Hoechst 33342. In conclusion, further study is needed to determine the optimum procedure and improve the nozzle to enhancing sorting accuracy or efficiency. Also, the findings of this study do not negate the possibility that the difference method of sperm sorting cannot use a UV laser beam.

Spray Characteristics in the cross region of twin spray between impinging F-O-O-F type injectors (충돌형 F-O-O-F 인젝터의 이중분무 중첩영역에서의 분무특성에 관한 연구)

  • Kwon, K.C.;Lee, E.S.;Kang, S.J.;Rho, B.J.
    • Proceedings of the KSME Conference
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    • 2001.11b
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    • pp.758-763
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    • 2001
  • This paper presents twin spray characteristics of two impinging F-O-O-F type injectors in which fuel and oxidizer impinge on each other to atomize under the various conditions. The droplet size and velocity in the impinging spray flow field were measured using a PDPA. The droplet size and velocity were investigated at mixture ratios of 1.5, 2.0, 2.47 and 3.0 for four injectors in which two single F-O-O-F injectors were arranged at intervals of 20.8, 31.2, 41.6 and 62.4mm respectively. In general, the arithmetic mean diameter, SMD and standard deviation of droplet size in the interaction area (X=0 and Y=0mm) were smaller. The axial velocity in the interaction area was slightly higher. Considering the behavior of impinged droplets using the We number calculated by using the axial velocity instead of the relative velocity in line C in Fig. 1(b) for four injectors, it is consumed that the We number over 500 had the possibility to disintegrate, and the We number below 500 had it to cohere after impingement of twin spray. The results of this study can be used for the design of a nozzle for liquid propellant rockets.

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Cumulative Distributions and Flow Structure of Two-Passage Shear Coaxial Injector with Various Gas Injection Ratio (2중 유로형 전단 동축 분사기의 기체 분사율에 따른 유동 및 입도분포)

  • Lee, Inchul;Kim, Dohun;Koo, Jaye
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.7
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    • pp.675-682
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    • 2013
  • To verify the effect of inner- and outer-stage gas jets, a shear coaxial injector was designed to analyze the axial velocity profile and breakup phenomenon with an increase in the measurement distance. When the measurement position was increased to Z/d=100, the axial flow showed a fully developed shape due to the momentum transfer, aerodynamic drag effect, and viscous mixing. An inner gas injection, which induces a higher momentum flux ratio near the nozzle, produces the greater shear force on atomization than an outer gas injection. Inner- and Outer-stage gas injection do not affect the mixing between the inner and outer gas flow below Z/d=5. The experiment results showed that the main effect of liquid jet breakup was governed by the gas jet of an inner stage. As the nozzle exit of the outer-stage was located far from the liquid column, shear force and turbulence breaking up of the liquid jets do not fully affect the liquid column. In the case of an inner-stage gas injection momentum flux ratio within 0.84, with the increase in the outer gas momentum flux ratio, the SMD decreases. However, at an inner-stage gas jet momentum flux ratio over 1.38, the SMD shows the similar distribution.

Numerical Study of Distribution Characteristics of Pulverized Coal According to Operation Condition in PM Burners (저공해 버너에서의 운전조건에 따른 미분탄 분배특성에 관한 수치 해석 연구)

  • Yoon, Sung-Hwan;Park, Jeong;Kwon, Oh-Boong;Park, Ho-Young;Seo, Sang-Il
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.35 no.5
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    • pp.491-501
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    • 2011
  • We performed numerical simulation using a DPM (discrete phase model) to identify the optimal operation ranges in two representative PM burners widely used in domestic 500-MW pulverized coal-fired power plants. Recently there has been an increased utilization of low-cost coals such as sub-bituminous coal. We investigate the effects of coal blends on the distribution ratio of coal to air by varying the mass flow rates of pulverized coal and primary air and the particle size. We present and discuss optimal conditions for the distribution ratio of coal to air in PM burners.

Combustion Characteristics of Fuel-rich Gas Generator with Impinging Injector for a Liquid Rocket Engine (액체로켓엔진에서 충돌형 분사기 형태의 연료과잉 가스발생기 연소특성)

  • Han, Yeoung-Min;Kim, Seung-Han;Lee, Kwang-Jin;Moon, Il-Yoon;Seol, Woo-Seok;Lee, Chang-Jin
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.33 no.6
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    • pp.64-70
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    • 2005
  • The overall results of hot firing tests of fuel-rich gas generator with impinging injector at design and off-design points are described. The gas generator consists of an injector head with impinging injector, a water cooled combustor wall, a turbulence ring to enhance mixing, an instrument ring measuring temperature and pressure and a nozzle. The combustion tests were successfully performed without damage of gas generator. Test results show that the outlet temperature is not dependent on residence time of hot gas within 4~6msec but dependent on chamber pressure. The relation between outlet temperature and combustion efficiency resulting from measured pressure, mass flow rate and area of nozzle throat is shown. The overall O/F ratio is the critical parameter to determine the outlet temperature and the linear correlation between two parameters is established.

Waterjet Propulsion Model Experiment for Catamaran Ship (쌍동선의 워터제트 추진 모형시험)

  • Choi, G.I.;Min, K.S.;Ann, Y.W.
    • Journal of the Society of Naval Architects of Korea
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    • v.33 no.1
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    • pp.65-76
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    • 1996
  • A screw propeller is usually accepted as a propulsor of many kinds of ships. However, for high speed vessels, screw propeller has large cavitation area on the blades so propeller efficiency is decreased and erosion can be happened. To avoid this problem, supercavitating propeller and waterjet are generally used for high speed vessels. In this paper, we introduced the self-propulsion test procedure which has been developed for high speed vessels in Hyundai Maritime Research Institute. The model ship used in experiment represents catamaran about 5.3 m in length. To minimize the experimental errors, two impellers were driven by a single motor. Thrust was calculated by converting the measured pressure to flow rates at the nozzle exit. The test procedure is composed of resistance test, self propulsion test and analysis. In order to measure the pressure, pressure tabs were installed around the nozzle exit and connected to the pressure sensor by vinyl tube.

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Thermal Energy Storage in Phase Change Material - by Means of Finned Thermosyphon - (상변화 물질을 이용한 에너지의 저장에 관한 연구 - 핀이 부착된 열싸이폰의 이용에 관하여 -)

  • Kim, Kwon-Jin;Yoo, Jai-Suk;Kim, Ki-Hyun
    • Solar Energy
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    • v.11 no.1
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    • pp.69-77
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    • 1991
  • A two-phase closed thermosyphon with circular fins was used as the heat transfer device for storing the thermal energy in paraffin wax. Experiments were carried out for 4, 6 and 8 fins and for various initial temperatures of the wax and power inputs. Heat transfer characteristics along the heat flow path were investigated as well as the overall performance of the system. Some of the important results are as follows:(1) The thermosyphon heat transfer coefficient and the overall heat transfer coefficient increased with the number of fins, whereas the heat transfer coefficient between the fin and the wax decreased; (2) Facilitation of heat transfer by the fins seemed to alleviate the dry-out phenomenon that had been reported to occur in case of bare thermosyphon; and (3) The horizontal fins had adverse effect of subduing a full scale convection in the wax, and the increase of the number of fins delayed the onset of local convection between the fins.

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Flow Characteristics of Two-Dimensional Turbulent Stepped Wall Jet (2次元 亂流 Stepped Wall Jet 의 流動特性)

  • 부정숙;김경천;박진호;강창수
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.9 no.6
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    • pp.732-742
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    • 1985
  • Measurements of mean velocity and turbulence characteristics are obtained with a linearized constant temperature hot-wire anemometer in a two-dimensional turbulent jet discharging parallel to a flate. Wall static pressure distribution is also measure. The Reynolds number based on the jet nozzle width (D) is about 42,000 and the step height is 2.5D. The reattachment length is found to be 7.5D by using both wool tuft and oil methods. Upstream of the reattachment point, there exist double coherent structures and mean velocity, Reynolds stresses and triple product profiles are asymmetric about jet center line due to the influence of streamline curvature and recirculating flow region. Near the reattachment point, wall static pressure and turbulence quantities change its shape rapidly because of the large eddies by the solid wall. Especially, turbulence intensity has a maximum value in the reattachment regin, then decreases slowly in the redeveloping wall jet ragion. Downstream of X/D=14, a single large scale eddy structure is formed. Far downstream affer the reattachment(X/D.geq.18) mean velocity profile, the decay of maximum velocity and the variation of jet half width are nearly similar to those of plane wall jet, but the Reynolds stresses are higher than those of the latter.

Development of a Parallel Cell-Based DSMC Method Using Unstructured Meshes (비정렬격자에서 병렬화된 격자중심 직접모사 기법 개발)

  • Kim, Hyeong-Sun;Kim, Min-Gyu;Gwon, O-Jun
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.30 no.2
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    • pp.1-11
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    • 2002
  • In the present study, a parallel DSCM technique based on a cell-based data structure is developed for the efficient simulation of rarefied gas flows especially od PC clusters. Dynamic load balancing is archieved by decomposing the computational domain into several sub-domains and accounting for the number of particles and the number cells of each domain. Mesh adaptation algorithm is also applied to improve the resolution of the solution and to reduce the grid dependency. It was demonstrated that accurate solutions can be obtained after several levels of mesh adapation starting from a coars initial grid. The method was applied to a two-dimensioanal supersonic leading-edge flow and the axi-symmetric Rothe nozzle flow to validate the efficiency of the present method. It was found that the present method is a very effective tool for the efficient simulation of rarefied gas flow on PC-based parallel machines.

Turbulent Dispersion Behavior of a Jet issued into Thermally Stratified Cross Flows (II) (열적으로 성충화된 횡단류에 분류된 제트의 난류확산 거동 (II))

  • Kim, Sang Ki;Kim, Kyung Chun
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.23 no.11
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    • pp.1434-1443
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    • 1999
  • The turbulent fluctuations of temperature and two components of velocity have been measured with hot- and cold-wires in the Thermally Stratified Wind Tunnel(TSWT). Using the fin-tube heat exchanger type heaters and the neural network control algorithm, both stable ($dT/dz=109.4^{\circ}C$) and unstable ($dT/dz=-49.1^{\circ}C$) stratifications were realized. An ambient air jet was issued normally into the cross flow($U_{\infty}=1.0 m/s$) from a round nozzle(d = 6 mm) flushed at the bottom waII of the wind tunnel with the velocity ratio of $5.8(U_{jet}/U_{\infty})$. The characteristics of turbulent dispersion in the cross flow jet are found to change drastically depending on the thermal stratification. Especially, in the unstable condition, the vertical velocity fluctuation increases very rapidly at downstream of jet. The fluctuation velocity spectra and velocity-temperature cospectra along the jet centerline were obtained and compared. In the case of stable stratification, the heat flux cospectra changes Its sign from a certain point at the far field because of the restratification phenomenon. It is inferred that the main reason in the difference between the vertical heat fluxes is caused by the different length scales of the large eddy motions. The turbulent kinetic energy and scalar dissipation rates were estimated using partially non-isotropic and isotropic turbulent approximation. In the unstable case, the turbulent energy dissipation decreases more rapidly with the downstream distance than in the stable case.