• 제목/요약/키워드: Two-dimensional temperature

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E. coli에서 근류균 섬유소 분해효소 유전자의 발현 및 생화학적 특성조사 (Expression and Biochemical Characterization of CMCase Gene of Rhizobium fredii Usda193 in Escherichia coli)

  • 윤호종;박용우;임선택;강규영;윤한대
    • 한국미생물·생명공학회지
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    • 제23권3호
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    • pp.275-281
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    • 1995
  • From the plasmid pYA300 carring a CMCase of Rhizobium fredii USDA193 plasmid was subcloned into pBluescript II KS(+)/pBluescript II SK(+) vectors and designated pYA500 and pYA600, respectively. Escherchia coli cells transformed with pYA500 porduced the CMCase more than with pYA600. The orientation of the cloned fragment in pBluescript vector had the effect on gene expression in E. coli background. When the 1.7 kb CMCase gene fragment of R. fredii USDA193 was hybridized to EcoRI-digested total DNA from R. meliloti and R. fredii USDA 191 the unique bands hybridized respectively, indicating that some genetic diversity exists in the EcoRI restriction enzyme site for CMCase gene in Rhizobium strains. The optimum pH of enzyme activity was 7 and the optimum temperature of that was nearly 37$\circ$C. The cellulase-minus derivatives of pYA500 were constructed by Tn5 insertional mutation. Among 6000 transconjugants, two mutant plasmids (designated pYA500::Tn5a and pYA500::Tn5b) were detected from the cellulase- negative transconjugants. The product of CMCase gene was analyzed by one dimensional SDS- PAGE of the cell extracts. About 45 kDa protein was considered to be a product of CMCase gene.

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Cholesteric 액정질서를 지닌 Hydroxypropyl Chitosan 가교필름의 제조와 팽윤거동 (Preparation and Swelling Behavior of Cross-Linked Films of Hydroxypropyl Chitosan Possessing Cholesteric Liquid-Crystalline Order)

  • 마영대;김경희
    • 폴리머
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    • 제24권3호
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    • pp.418-430
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    • 2000
  • 알칼리키토산과 프로필렌옥사이드를 반응시킴에 의해 유방성 및 열방성 액정상을 형성하는 새로운 히드록시프로필키토산(HPCTO)을 합성함과 동시에 HPCTO/메탄올의 유방성 액정용액에 glyoxal첨가하여 주형시킴에 의해 HPCTO의 가교필름들을 제조하였다. 가교필름들의 열 및 팽윤 특성을 검토하였다. 필름들은 cholesteric 액정상의 특징적인 지문조직을 나타내었으며 이들의 pitch는 온도 및 가교제의 농도가 증가할수록 증가하였다. 가교시료들은 물과 메탄을 중에서 이방성 팽윤을 나타내었으며, 이러한 사실은 HPCTO분자들간에 2차원적인 가교가 우선적으로 일어남을 시사한다. 이방성 팽윤의 정도는 용매의 종류에 크게 의존하나 검토된 가교제의 농도에는 의존하지 않았다.

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Optical properties of a-plane InGaN/GaN multi-quantum wells with green emission

  • Song, Hoo-Young;Kim, Eun-Kyu;Lee, Sung-Ho;Hwang, Sung-Min
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2009년도 제38회 동계학술대회 초록집
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    • pp.172-172
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    • 2010
  • In the area of optoelectronic devices based on GaN and related ternary compounds, the two-dimensional system like as quantum wells (QWs) has been investigated as an effective structure for improving the light-emitting efficiency. Generally, the quantum well active regions in III-nitride light-emitting diodes grown on conventional c-plane sapphire substrates have critical problems given by the quantum confined Stark effect (QCSE) due to the effects of strong piezoelectric and spontaneous polarizations. However, the QWs grown on nonpolar templates are free from the QCSE since the polar-axis lies within the growth plane of the template. Also the unique characteristic of linear polarized light emission from nonpolar QW structures is attracting attentions because it is proper to the application of back-light units of liquid crystal display. In this study, we characterized optical properties of the a-plane InGaN/GaN QW structures by temperature-dependent photoluminescence (TDPL) measurements. From the photoluminescence (PL) spectrum measured at 300 K, green emission centered at 520 nm was observed for the QW region. Since indium incorporation on nonpolar QWs is lower than that on c-plane, this high indium-doping on a-plane InGaN QWs is not common. Therefore, the effect of high indium composition on optical properties in a-plane InGaN QWs will be extensively studied.

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Constructing a Three-Dimensional Endothelial Cell Layer in a Circular PDMS Microchannel

  • Choi, Jong Seob;Piao, Yunxian;Kim, Kyung Hoon;Seo, Tae Seok
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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    • pp.274.2-274.2
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    • 2013
  • We described a simple and efficient fabrication method for generating microfluidic channels with a circular-cross sectional geometry by exploiting the reflow phenomenon of a thick positive photoresist. Initial rectangular shaped positive photoresist micropatterns on a silicon wafer, which were fabricated by a conventional photolithography process, were converted into a half-circular shape by tuning the temperature to around $105^{\circ}C$. Through optimization of the reflow conditions, we could obtain a perfect circular micropattern of the positive photoresist, and control the diameter in a range from 100 to 400 ${\mu}m$. The resultant convex half-circular photoresist was used as a template for fabricating a concave polydimethylsiloxane (PDMS) through a replica molding process, and a circular PDMS microchannel was produced by bonding two half-circular PDMS layers. A variety of channel dimensions and patterns can be easily prepared, including straight, S-curve, X-, Y-, and T-shapes to mimic an in vivo vascular network. To inform an endothelial cell layer, we cultured primary human umbilical vein endothelial cells (HUVECs) inside circular PDMS microchannels, and demonstrated successful cell adhesion, proliferation, and alignment along the channel.

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The study of the calculation of energy consumption load for heating and cooling in building using the Laplace Transform solution

  • Han, Kyu-Il
    • 수산해양기술연구
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    • 제50권3호
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    • pp.292-300
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    • 2014
  • The Laplace Transform solution is used as a mathematical model to analyse the thermal performance of the building constructed using different wall materials. The solution obtained from Laplace Transform is an analytical solution of an one dimensional, linear, partial differential equation for wall temperature profiles and room air temperatures. The main purpose of the study is showing the detail of obtaining solution process of the Laplace Transform. This study is conducted using weather data from two different locations in Korea: Seoul, Busan for both winter and summer conditions. A comparison is made for the cases of an on-off controller and a proportional controller. The weather data are processed to yield hourly average monthly values. Energy consumption load is well calculated from the solution. The result shows that there is an effect of mass on the thermal performance of heavily constructed house in mild weather conditions such as Busan. Building using proportional control experience a higher comfort level in a comparison of building using on-off control.

Transformation of TiO2 Film to Titanate Nanotube Thin Film Using Hydrothermal Method

  • Guo, Yupeng;Lee, Nam-Hee;Oh, Hyo-Jin;Yoon, Cho-Rong;Kim, Sun-Jae
    • 한국표면공학회:학술대회논문집
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    • 한국표면공학회 2007년도 춘계학술발표회 초록집
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    • pp.147-148
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    • 2007
  • In this study, the technology to grow oriented nanotube thin film from dip-coated $TiO_2$ using hydrothermal method has been successfully developed. The effects of preparation parameters, such as reaction temperature, duration and post treatment conditions on the film morphologies and the adherence to the substrate, have been examined. A general formation mechanism of oriented titanate nanotube thin film is proposed in terms of the detailed observation of the products via two dimensional surface FESEM studies and HRTEM images. The overall formation of $TiO_2-based$ nanotube thin film can be summarized with three successive steps: (1) $TiO_2$ dissolving and amorphous $Na_2TiO_3$ deposition process; (2) layered $Na_2Ti_3O_7$ formation via spontaneous crystallization and rapid growth process; (3) formation of nanotube thin film via $Na_2Ti_3O_7$ splitting and multilayer scrolling process of (100) planes around the c axis of $Na_2Ti_3O_7$.

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REDUCED DIFFERENTIAL TRANSFORM FOR THERMAL STRESS ANALYSIS UNDER 2-D HYPERBOLIC HEAT CONDUCTION MODEL WITH LASER HEAT SOURCE

  • SUTAR, CHANDRASHEKHAR S.;CHAUDHARI, KAMINI K.
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • 제25권2호
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    • pp.54-65
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    • 2021
  • In this study, a two-dimensional thermoelastic problem under hyperbolic heat conduction theory with an internal heat source is considered. The general solution for the temperature field, stress components and displacement field are obtained using the reduced differential transform method. The stress and displacement components are obtained using the thermal stress function in the reduced differential transform domain. All the solutions are obtained in the form of power series. The special case with a time-dependent laser heat source has been considered. The problem is considered for homogeneous material with finite rectangular cross-section heated with a non-Gaussian temporal profile. The effect of the heat source on all the characteristics of a material is discussed numerically and graphically for magnesium material taking a pulse duration of 0.2 ps. This study provides a powerful tool for finding the solution to the thermoelastic problem with less computational work as compared to other methods. The result obtained in the study may be useful for the investigation of thermal characteristics in engineering and industrial applications.

Numerical Analysis of Flow Uniformity in Selective Catalytic Reduction (SCR) Process Using Computational Fluid Dynamics (CFD)

  • Shon, Byung-Hyun
    • International Journal of Advanced Culture Technology
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    • 제10권3호
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    • pp.295-306
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    • 2022
  • The NOx removal performance of the SCR process depends on various factors such as catalytic factors (catalyst composition, shape, space velocity, etc.), temperature and flow rate distribution of the exhaust gas. Among them, the uniformity of the flow flowing into the catalyst bed plays the most important role. In this study, the flow characteristics in the SCR reactor in the design stage were simulated using a three-dimensional numerical analysis technique to confirm the uniformity of the airflow. Due to the limitation of the installation space, the shape of the inlet duct was compared with the two types of inlet duct shape because there were many curved sections of the inlet duct and the duct size margin was not large. The effect of inlet duct shape, guide vane or mixer installation, and venturi shape change on SCR reactor internal flow, airflow uniformity, and space utilization rate of ammonia concentration were studied. It was found that the uniformity of the airflow reaching the catalyst layer was greatly improved when an inlet duct with a shape that could suppress drift was applied and guide vanes were installed in the curved part of the inlet duct to properly distribute the process gas. In addition, the space utilization rate was greatly improved when the duct at the rear of the nozzle was applied as a venturi type rather than a mixer for uniform distribution of ammonia gas.

기저부 경사각과 깊이가 에너지 제어형 용착 공정으로 보수된 영역의 열 및 잔류응력 특성에 미치는 영향 고찰 (A Study on the Influence of the Inclined Angle and Depth of the Substrate on Thermal and Residual Stress Characteristics in the Vicinity of the Repaired Region by a Directed Energy Deposition Process)

  • 김단아;이광규;안동규
    • 한국기계가공학회지
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    • 제21권6호
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    • pp.50-59
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    • 2022
  • The design of the substrate significantly affects the thermal history and the residual stress formation in the vicinity of a repaired region by a directed energy deposition (DED) process. The occurrence of defects in the repaired region depends on the thermal history and residual stress formation. The objective of this study was to investigate the influence of the inclined angle and depth of the substrate on the thermal and residual stress characteristics in the vicinity of a repaired region by a DED process through two-dimensional finite element analyses (FEAs). The temperature and residual stress distributions in the vicinity of the repaired region were predicted according to the combination of the inclined angle and depth of the substrate. The effects of the inclined angle and depth on the depth of the heat affected zone and the maximum value of the residual stress were examined. A proper combination of the inclined angle and depth of the substrate was estimated to decrease the residual stress in the vicinity of the repaired region.

Representation of fundamental solution and vibration of waves in photothermoelastic under MGTE model

  • Rajneesh Kumar;Nidhi Sharma;Supriya Chopra;Anil K. Vashishth
    • Ocean Systems Engineering
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    • 제13권2호
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    • pp.123-146
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    • 2023
  • In this paper, Moore-Gibson-Thompson theory of thermoelasticity is considered to investigate the fundamental solution and vibration of plane wave in an isotropic photothermoelastic solid. The governing equations are made dimensionless for further investigation. The dimensionless equations are expressed in terms of elementary functions by assuming time harmonic variation of the field variables (displacement, temperature distribution and carrier density distribution). Fundamental solutions are constructed for the system of equations for steady oscillation. Also some preliminary properties of the solution are explored. In the second part, the vibration of plane waves are examined by expressing the governing equation for two dimensional case. It is found that for the non-trivial solution of the equation yield that there exist three longitudinal waves which advance with the distinct speed, and one transverse wave which is free from thermal and carrier density response. The impact of various models (i)Moore-Gibson-Thomson thermoelastic (MGTE)(2019), (ii) Lord and Shulman's (LS)(1967) , (iii) Green and Naghdi type-II(GN-II)(1993) and (iv) Green and Naghdi type-III(GN-III)(1992) on the attributes of waves i.e., phase velocity, attenuation coefficient, specific loss and penetration depth are elaborated by plotting various figures of physical quantities. Various particular cases of interest are also deduced from the present investigations. The results obtained can be used to delineate various semiconductor elements during the coupled thermal, plasma and elastic wave and also find the application in the material and engineering sciences.