• Journal of Broadcast Engineering
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• v.20 no.4
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• pp.509-520
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• 2015

A design for the merge mode of three dimensional High Efficiency Video Coding (3D-HEVC) is proposed in this paper. The proposed design can reduce the implementation complexity by removing the duplicated modules of the HEVC. For the extension codec, the implementation complexity is as crucial as coding efficiency, meaning if possible, extension codec needs to be easily implemented through by reusing the design of the legacy codec as-is. However, the existing merging process of 3D-HEVC had been built-in integrated in the inside of the HEVC merging process. Thus the duplicated merging process of HEVC had to be fully re-implemented in the 3D-HEVC. Consequently the implementation complexity of the extension codec was very high. The proposed 3D-HEVC merge mode is divided into following two stages; the process to reuse the HEVC modules without any modification; and the reprocessing process for newly added and modified merging modules in 3D-HEVC. By applying the proposed method, the re-implemented HEVC modules, which accounted for 51.4% of 3D-HEVC merge mode confirmed through the operational analysis of algorithm, can be eliminated, while maintaining the same coding efficiency and computational complexity.

• The Journal of Engineering Geology
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• v.9 no.3
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• pp.187-206
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• 1999

A computer program to numerically predict the permeability tensor of fractured rocks is developed using information on discontinuities which Borehole Televiewer and Borehole Image Processing System (BIPS) provide. It uses orientation and thickness of a large number of discontinuities as input data, and calculates relative values of the 9 elements consisting of the permeability tensor by the formulation based on the EPM model, which regards a fractured rock as a homogeneous, anisotropic porous medium. In order to assess feasibility of the program on field sites, the numerically calculated tensor was obtained using BIPS logs and compared to the results of pumping test conducted in the boreholes of the study area. The degree of horizontal anisotropy and the direction of maximum horizontal permeability are 2.8 and $N77^{\circ}CE$, respectively, determined from the pumping test data, while 3.0 and $N63^{\circ}CE$ from the numerical analysis by the developed program. Disagreement between two analyses, especially for the principal direction of anisotropy, seems to be caused by problems in analyzing the pumping test data, in applicability of the EPM model and the cubic law, and in simplified relationship between the crack size and aperture. Aside from these problems, consideration of hydraulic parameters characterizing roughness of cracks and infilling materials seems to be required to improve feasibility of the proposed program. Three-dimensional assessment of its feasibility on field sites can be accomplished by conducting a series of cross-hole packer tests consisting of an injecting well and a monitoring well at close distance.

• The Journal of Engineering Geology
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• v.22 no.4
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• pp.409-416
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• 2012

In this study, an electrical resistivity survey and a drilling investigation were conducted at an animal carcass disposal site. Chemical analysis of leachate collected from the site was also performed (sampling times: May 2011 and June 2012). Five lines of dipole-dipole electrical resistivity surveys were carried out, along with drilling investigations at 3 points within the disposal areas and 11 points near the disposal site. Two-dimensional inverse modeling of the collected resistivity data was performed to evaluate the properties (size, depth, and form) of the disposal site. Leachate analysis showed that pH of leachate decreased from 7.4 to 6.7, while Eh changed from -358 mV to -48 mV over time. In addition, dissolved ions increased due to the progression of carcass decomposition. Results of the electrical resistivity survey indicated that low resistivity zones (minimum value, $0.64{\Omega}m$) existed at a depth of 8 m from the surface. Considering the bedrock location and carcass disposal depth, there was no evidence of bedrock contamination by leachate. The results of the electrical resistivity survey are consistent with those of the drilling investigation, which indicates that electrical resistivity effectively depicted the properties of the disposal site. This study demonstrates that electrical resistivity survey is a suitable technique for investigation of animal carcass disposal sites.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.2
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• pp.99-106
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• 2011

In order to investigate rice stem proteome in response to heat stress, rice plants were subjected to heat treatment at 42$^{\circ}C$ and total soluble proteins were extracted from stem tissues, and were fractionated with 15% PEG (poly ethylene glycol) and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). After staining of 2-DE gels, 46 of differentially expressed proteins were extracted, digested by trypsin, and subjected to matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Proteins were identified through database search by using peptide mass fingerprints. Among them, 10 proteins were successfully identified. Seven proteins were up- and 3 proteins were down-regulated, respectively. These proteins are involved in energy and metabolism, redox homeostasis, and mitochondrial small heat shock proteins. The identification of some novel proteins in the heat stress response provides new insights that can lead to a better understanding of the molecular basis of heat-sensitivity in plants, and also useful to molecular breeding of thermotolerant forage crops.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.1
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• pp.53-61
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• 2020

Cell migration is a central process for recovering from wounds triggered by physical distress besides embryogenesis and cancer metastasis. Wound healing assay is widely used as a fundamental research technique for investigation of two-dimensional cell migration in vitro. The most common approach for imitating physical wound in vitro is mechanical scratching on the surface of the confluent monolayer by using sharp materials. The iron metal pin with a suspension spring for fine adjustment of the orthogonal contact surface between the scratching point and the individual bottom of multi-well plate with planar curvatures were adopted for the creative invention of a 96-well plate wound maker. While classic tips drew diverse and zigzag scratching patterns on the confluent monolayer, our wound maker displayed synchronized linear wounds in the middle of each well of a 96-well plate that was seeded with several cell lines. Given that several types of multi-well plates commercially available are compatible with our lab-made wound maker for creating uniform scratches on the confluent monolayer for the collective cell migration in wound healing assay, it is certain that the application of this wound maker to the real-time wound healing assay in high content screening (HCS) is superior than utilization of typical polypropylene pipette tips.

• Fire Science and Engineering
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• v.20 no.2 s.62
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• pp.44-53
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• 2006

We have conducted a numerical simulation under two-dimensional unsteady conditions in order to analyze the effect according to the injection angle and velocity of the $CO_2$ agent nozzle which is one of the elements for the fixed type $CO_2$ fire extinguishing system installed in a ship on the characteristics of flow and $CO_2$ concentration distribution. The flow fields and concentration fields were measured and analyzed. We can found that the difference of flow patterns according to the conditions of $CO_2$ agent injection nozzle, and in all the conditions of $CO_2$ agent injection nozzle, the iso-concentration line was expanded from the region at which vortex was generated to the surroundings. We can expected that the intensity of the wall jet on the bottom floor was generated differently and the iso-concentration lines were expanded or shrunk according to the angle of $CO_2$ agent injection nozzle. In case of increasing $CO_2$ agent injection velocity maintaining the flow quantity of the $CO_2$ agent injection equally, the iso-concentration line of $CO_2$ agent on bottom floor can be formed more higher than in case of decreasing $CO_2$ agent injection velocity.

• The Korean Journal of Pharmacology
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• v.32 no.2
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• pp.243-257
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• 1996

CCK and cholinergic agonist stimulate enzyme release from the pancreatic acini via G-protein-mediated activation of phospholipase C, In contrast secretin and related peptides increase the level of cAMP and activate cAMP-dependent protein kinase. Camostat, a synthetic protease inhibitor, causes pancreatic hypertrophy and hyperplasia by increasing the CCK release. In this study, the secretagogue-induced changes of intracellular proteins were examined in the dispersed pancreatic acini of rats with or without camostat treatment. Camostat(FOY-305, 200 mg/kg, p.o.) was given for 4 days twice daily and the dispersed acini were prepared at 12 bouts after last treatment. The profiles of Intracellular phosphoproteins were analyzed by two-dimensional gel electrophoresis after incubating the acini with $^{32}P$. The amylase release from the dispersed acini was measured. The pancreatic weight was increased to 126% of control, while amylase activity per mg acinar protein decreased to 41% of control, The maximum response of amylase release from dispersed acini to CCK-8 or carbachol was markedly decreased(65% or 46% of control, respectively). The group of intracellular proteins(24 kD, pI $4.5{\sim}8.5$) was increased in quantity by camostat. CCK-8 or secretin increased phosphorylation of a protein(34 kD, pI 4.7) in camostat-treated as well as control rats. CCK-8 increased tyrosine phosphoryiation in the acini of control rats. However, in camostat-treated rats, the basal level of tyrosine phosphorylation was increased and it was rather decreased by CCK-8. Secretin had no effect on the level of tyrosine phosphorylation in acini. These results indicate that both phospholipase C and adenylate cyclase induce phosphorylation of an intracellular acinar protein(34 kD, pI 4.7) and camostat treatment increases the basal level of tyrosine phosphorylation in acinar cells. And these results suggest that not only serine/threonine protein kinase but also protein tyrosine kinase/phosphatase are involved in the process of CCK receptor mediated stimulation-secrelion coupling.

• Journal of Life Science
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• v.14 no.2
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• pp.325-330
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• 2004

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (Homologue of RAD3 gene). The over-expressed HRD3 protein was estimated to be a 75 kDa in size which is in good agreement with the estimated by the nucleotide sequence of the cloned gene. Two-dimensional gel electrophoresis showed that a number of other protein spots dramatically disappeared when the HRD3 protein was overexpressed. The overexpressed RAD3 protein showed a toxicity in E. coli host, suggesting that this protein may be involved in the inhibition of protein synthesis and/or degradation of host protein. To determine which part of HRD3 gene contributes to the toxicity in E. coli, various fusion plasmids containing a partial sequence of HRD3 and lac'Z gene were constructed. These results suggest that the C-terminal domain of HRD3 protein may be important for both toxic effect in E. coli and for its role in DNA repair in S. pombe.

• Journal of KIISE:Software and Applications
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• v.33 no.2
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• pp.220-230
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• 2006

As the computing technique and mobile devices get developed, the demand of multimedia contents for mobile environment has been increased. The multimedia contents provided on PDA has so far been limited to the materials such as video and audio. MPEG-4 is the international standard used for supporting to properly save, communicate multimedia in formation such as video, audio, image, text and two-dimensional object, which can present various, multimedia contents by using adaptation techniques. However, since most MPEG-4 contents are not used for PDA but desktop, it seems not to be played on PDA where needs low power consumption, limited memory capability and GUI, and so on. In this paper, we propose the adaptation techniques, which can present the MPEG-4 contents on PDA, using scene composition with MPEG-4. The proposed scheme consists of three subparts: physical adaptation, variation adaptation and resource adaptation. Physical adaptation adjusts the physical difference of between the authoring environment and playback environment. Event adaptation part transforms events used for desktop to the events used for playback on PDA. The resource adaptation enables efficiency of playback to promote by using the essential information table on BIFS parser. As the proposed scheme is applied to MPEG-4 player, we see that the MPEG-4 contents are efficiently played on PDA.

• Journal of the Korean GEO-environmental Society
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• v.16 no.9
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• pp.33-41
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• 2015

The vertical bearing capacity of a bucket foundation installed in sand can be calculated as sum of the skin friction and end bearing capacity. However, the current design equations are not considering the non-associated flow characteristics of sand and the reduction in the skin friction and increase in the end bearing capacity when the vertical load is applied. In this study, we perform two-dimensional axisymmetric finite element analyses following non-associated flow rule and calculate the vertical bearing capacity of circular bucket foundation of various sizes installed in sand of different friction angles. After calculating the skin friction and end bearing force at the ultimate state, design equations are derived for each. The skin friction of bucket foundation is shown significantly small compared to the end bearing capacity. Considering the difference with the available design equation for piles, it is recommended that the equation for piles is used for the bucket foundation. A new shape-depth factor ($s_q{\cdot}d_q$) for bucket foundation is recommended which also accounts for the increment of the end bearing capacity due to skin friction. Additionally, the shape and depth factor of embedded foundation proposed from the associated flow rule can overestimate the bearing capacity in sand, so it is more adequate to use the shape-depth factor proposed in this study.