• Journal of the Korean Society of Propulsion Engineers
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• v.12 no.3
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• pp.68-75
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• 2008

The three-dimensional structure of detonation wave propagating in a circular tube was investigated using a parallel computational code developed previously. A series of parametric study for a circular tube of a fixed diameter gave the formation mechanism of the detonation cell structures depending on pre-exponential factor, k. The unsteady results in three-dimension showed the mechanisms of two, three and four cell mode of detonation wave front structures. The detonation cell number was increased but cell width and length were decreased with increased pre-exponential factor k. In the all multi-cell mode, the detonation wave structure and smoked-foil records on the wall are made by the moving of transverse waves. The detonation wave front structures have the regular polygon and windmill shapes periodically.

• Molecules and Cells
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• v.38 no.1
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• pp.14-19
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• 2015

Eph receptors and their ligands, ephrins, represent the largest group of the receptor tyrosine kinase (RTK) family, and they mediate numerous developmental processes in a variety of organisms. Ephrins are membrane-bound proteins that are mainly divided into two classes: A class ephrins, which are linked to the membrane by a glycosylphosphatidylinositol (GPI) linkage, and B class ephrins, which are transmembrane ligands. Based on their domain structures and affinities for ligand binding, the Eph receptors are also divided into two groups. Trans-dimerization of Eph receptors with their membrane-tethered ligands regulates cell-cell interactions and initiates bidirectional signaling pathways. These pathways are intimately involved in regulating cytoskeleton dynamics, cell migration, and alterations in cellular dynamics and shapes. The EphBs and ephrinBs are specifically localized and modified to promote higher-order clustering and initiate of bidirectional signaling. In this review, we present an in-depth overview of the structure, mechanisms, cell signaling, and functions of EphB/ephrinB in cell adhesion and migration.

• Journal of Breast Disease
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• v.6 no.2
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• pp.39-45
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• 2018

Purpose: Dieckol, a phlorotannin compound isolated from Ecklonia cava, has been reported to have antioxidant, antiviral, anti-inflammatory, and anticancer properties. The purpose of this study was to investigate its anticancer effects on human breast cancer cell lines. Methods: In this study, the viability of two human breast cancer cell lines SK-BR-3 and MCF-7 was investigated after dieckol treatment using a WST-1 assay. Apoptosis and cell cycle distribution were assayed via Annexin V-fluorescein isothiocyanate and propidium iodide staining followed by flow cytometric analysis. Immunoblotting analysis was also performed using Bax/Bcl-2 to determine whether the dieckol-induced apoptosis was mediated by the intrinsic apoptotic pathway. Results: In a dose dependent manner, dieckol reduced the number of viable cells and increased the number of apoptotic cells. The effect of dieckol on the cell cycle distribution was analyzed using flow cytometry. Dieckol treatment significantly increased the percentage of MCF-7 and SK-BR-3 in the G2/M phase. Immunoblot analysis revealed that 24 hours of dieckol exposure increased the Bax/Bcl-2 ratio. Conclusion: Dieckol induced cytotoxicity in MCF-7 and SK-BR-3 human breast cancer cells inducing apoptosis and cell cycle arrest. Therefore, it is suggested that dieckol may be a potential therapeutic agent for breast cancer.

• Fisheries and Aquatic Sciences
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• v.19 no.4
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• pp.16.1-16.8
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• 2016

To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro. Subsequently, determination of growth conditions, long-term culture, characterization, and cryopreservation of the cell lines were also conducted. Five gonad-derived cell lines were stably established and cultured continuously over at least the 73th passage and 402 culture days under the media containing 20 % fetal bovine serum at $28^{\circ}C$. All cell lines consisted of two main cell types based on morphology even if the ratio of the two cell types was different depending on cell lines. Despite long-term culture, all cell lines maintained diploid DNA contents and expression of several genes that are known to express in the A. baerii gonad. After freezing and thawing of the cell lines, post-thaw cell viabilities between 57.6 and 92.9 % depending on cell lines were indentified, suggesting that stable cryopreservation is possible. The results and the cell lines established in this study will contribute to the development of an in vitro system for A. baerii germline stem cell culture.

• Journal of Microbiology
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• v.39 no.1
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• pp.74-78
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• 2001

The transcription of the chitin synthase genes (chss) was cell cycle-regulated in Aspergillus nidulans and the expression pattern was classified into two groups. Group one, containing chsA and chsC, showed decreasing transcription level upon entry into the S-phase and no further variation during the remainder of the cell cycle. However, group two, containing chsB, chsD, and csmA showed a sharp decrease of mRNA level upon entry into the G2-phase and an increase during the M-phase. Our results suggested that the chss, belonging to same group with the similar expression pattern during the cell cycle are functionally linked and that chsD may play a role in hyphal growth and development in A. nidulans.

• Proceedings of the KIEE Conference
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• 1999.07d
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• pp.1544-1546
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• 1999

The fabrication process for ceramic fuel cell with a large electrode area was investigated. A cofired cell of two layer, electrolyte/anode, yielded a power of $200mW/cm^2$. Its performance loss was mainly due to iR drop in the anode side. The performance of the cofired of three layer. cathode/electrolyte/ anode, was much lower than that of two layer, which resulted from the large iR drop and overvoltage at the cathode side. Also a flat cell with a large area of $7.7{\times}10.8cm^2$ was fabricated successfully and tested using ceramic and metallic interconnectors. The large cell with metallic interconnectror showed a good performance of 0.6 V, 4.5 A.

• IE interfaces
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• v.15 no.4
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• pp.364-373
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• 2002

This paper developed an operating software for the manufacturing cell of a model plant. The model plant, which was manufactured by Fishertechnik, consists of an assembly cell, a machining cell, and supplementary material handling equipments. Each I/O module of the model plant communicates with the INTERBUS controller devices via High-level Language Interface(HLI). The machining center has two machining cells, which consists of 4 machines respectively, two 3-axis portal transporters, and a rail guided vehicle(RGV). The Petri-Net was used to investigate the dynamics of each machining cell, e.g., the relation of material handling equipments and machines. The operating software was analyzed and designed by Object Oriented Technique. The software was implemented using Delphi 3.0 under Windows 95/NT operating system.

• Reproductive and Developmental Biology
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• v.36 no.3
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• pp.155-161
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• 2012

Germ cell-specific hyaluronidases such as sperm adhesion molecule 1 (SPAM1) and hyaluronoglucosaminidase 5 (Hyal5) are in part responsible for dispersal of the cumulus cell mass, which is a critical step in establishing fertilization in mammals. In this study, we identified two testis-hyaluronidases, SPAM1 and Hyal5, in hamster and rat. These two genes were expressed specifically in the testis. At the protein level, hamster SPAM1 and Hyal5 display 78.7% and 75.4% identity with mouse SPAM1 and Hyal5. Further, the activity of the enzymes with respect to cumulus cell dispersion did not differ, although we observed that the enzymatic activity differed in pH range. These studies suggest that different sperm hyaluronidases are capable of dispersing the cumulus cell mass despite differences in enzyme activity.

• Journal of Information Display
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• v.9 no.2
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• pp.22-27
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• 2008

We propose an optical configuration of a vertical alignment (VA) liquid crystal (LC) cell to eliminate the light leakage in the diagonal direction. VA LC cell has an excellent contrast ratio in the normal direction due to the no phase-retardation. However, change of the phase-retardation occurs in all directions, which causes the light leakage and deteriorates the characteristics of the dark state. We designed the LC cell structure composed of multiple combinations with two A-plates and two C-plates in order to achieve wide viewing property on the Poincare sphere. From calculations, we show that the proposed structure can improve the viewing angle characteristics by compensating for the light leakage in all directions.

• Proceedings of the KIEE Conference
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• 1994.07b
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• pp.1336-1338
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• 1994

Cell fusion device is an artificial equipment which fuses electrically two types of cells fed from the respective micropump to the fusion chamber by electric pulses. In this case, the detective sensor of flowing cell, along with passage, is required to control the time of pulses applied to cell and the injection of cells which are fed from inlet to micropump. There are two methods of detection of flowing cell; optical, impedance method. The difference of output for optical sensor is about 426mV for 805nm wavelength. about 37mV for 665nm wavelength. In impedance method, sensor output is 132.33mV at middle point and 117.10mV at edge point in the channel. Experimental results show that the optimal frequency range of sensor output is Iron 50Hz to 400Hz.