• Microbiology and Biotechnology Letters
• /
• v.21 no.4
• /
• pp.336-341
• /
• 1993

In metal-affinify aqueous two-phase systems, protein partitioning is affected by a variety of parameters such as pH, the number of surface-accessible histidines, and the amount and partition coefficient of metallated polythylene glyco(PEG) ligand. To enhance partitioning of proteins with surface-accessible histidines, we have synthesized and used a (Cu(II)-ininodiacetic acid)$_2$-PEG20,000($Cu(II)_2IDA_2$-PEG20,000) as well as Cu(II)IDA-PEG5,000 as an affinity ligand. The partition coefficient of $Cu(II)_2-IDA_2$-PEG20,000 in a PEG5,000/dextran two-phase system was 30.1, which corresponded to a 3.8-fold increase over that of Cu(II)IDA-PEG5,000. The partitioning experiments were performed on four proteins, horse cytochrome c, S. cerevisiae cytochrome c, horse myoglobin, and sheep myoglobin. Partitioning of proteins which convey surface-accessible histidines was enhanced dramatically by the addition of $Cu(II)_2IDA_2$-PEG20,000 ligand. These results demonstrate that enhanced partitioning of metal-binding proteins in an aqueous two -phase system can by achieved by using an appropriate metallated PEG ligand.

• ETRI Journal
• /
• v.18 no.3
• /
• pp.147-160
• /
• 1996

We investigate noise-induced phase transitions in globally coupled active rotators with multiplicative and additive noises. In the system there are four phases, stationary one-cluster, stationary two-cluster, moving one-cluster, and moving two-cluster phases. It is shown that multiplicative noise induces a bifurcation from one-cluster phase to two-cluster phase. Pinning force also induces a bifurcation from moving phase to stationary phase suppressing the multiplicative noise effect. Additive noise reduces both effects of multiplicative noise and pinning force urging the system to the stationary one-cluster phase. The frustrated effects of pinning force and additive and multiplicative noises lead to a reentrant transition at intermediate additive noise intensity. Nature of the transition is also discussed.

• Journal of KSNVE
• /
• v.6 no.2
• /
• pp.163-177
• /
• 1996

An investigation on thermohydraulic stability of flow oscillations in the CANada Deuterium Uranium-600(CANDU-6) heat transport system has been conducted. Flow oscillations in reactor coolant loops, comprising two heat sources and two heat sinks in series, are possibly caused by the response of the pressure to extraction of fluid in two-phase region. This response consists of two contributions, one arising from mass and another from enthalpy change in the two-phase region. The system computer code used in the investigation os SOPHT, which is capable of simulating steady states as well as transients with varying boundary conditions. The model was derived by linearizing and solving one-dimensional, homogeneous single- and two-phase flow conservation equations. The mass, energy and momentum equations with boundary conditions are set up throughout the system in matrix form based on a node-link structure. Loop stability was studied under full power conditions with interconnecting the two compressible two phase regions in the figure-of-eight circuit. The dominant function of the interconnecting pipe is the transfer of mass between the two-phase regions. Parametric survey of loop stability characteristics, i. e., damping ratio and period, has been made as a function of geometrical parameters of the interconnection line such as diameter, length, height and orifice flow coefficient. The stability characteristics with interconnection line has been clarified to provide a simple criterion to be used as a guide in scaling of the pipe.

• Journal of the Institute of Convergence Signal Processing
• /
• v.14 no.1
• /
• pp.39-44
• /
• 2013

In this paper digital phase-shifter for multi-arm spiral antennas was designed by using Hilbert transform. All frequency components in input signal are phase-shifted for 90 degree by Hilbert transform, and the transform is implemented by FIT and IFIT. Digital phase-shifter generates two signals with phase difference of 90 degree by using Hilbert transform from input signals sampled by analog-digital converter(ADC), and then the input signal is phase-shifted for a given phase by using two signals. Hilbert transform based on digital phase-shifter is designed by Xilinx System generator, and the effects of input noise, FIT point, sampling period, initial phase of input signal, and shifted phase are simulated and its results are compared with Matlab results.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.28 no.5
• /
• pp.115-121
• /
• 2014

The intrinsic simplicity, ruggedness, and simple power electronic drive requirement of a Switched Reluctance Motor(SRM) make it possible to use in many commercial adjustable speed application. The simple magnetic circuit results in a high efficiency drive and low temperature rise, and the drive system provides a good drive characteristics. This paper is provides two phase 4/3 SRM that is similar to two phase 6/3 SRM as aspect to magnetic structure. Although 6/3 SRM does not experience any flux reversal as the flux is in the same direction whether phase A or B is excited, but two phase 4/3 SRM experiences a flux reversal in small part of stator yoke. The flux reversal in two phase 4/3 SRM could be relieved by an adjustment of stator yoke structure. The magnetic analysis and design considerations of the two phase 4/3 SRM have been obtained by the finite element method analysis (FEM).

• Journal of Power Electronics
• /
• v.17 no.5
• /
• pp.1256-1267
• /
• 2017

A fault tolerant structure and its corresponding control strategy for electromagnetic stirring power supplies are proposed in this paper. The topology structure of the electromagnetic stirring power supply contains two-stages. The fore-stage is the PWM rectifier. The back-stage is the fault tolerant inverter, which is a two-phase three-bridge orthogonal inverter circuit while operating normally. When the power switch devices in the inverter are faulty, the structure of the inverter is reconfigured. The two-phase half bridge inverter circuit is constructed with the remaining power switch devices and DC-link capacitors to keep the system operating after cutting the faulty power switch devices from the system. The corresponding control strategy is proposed to let the system work under both normal and fault conditions. The reliability of the system is improved and the requirement of the electromagnetic stirring process is met. Finally, simulation and experimental results verify the feasibility of the proposed fault tolerant structure and corresponding control strategy.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.15 no.1
• /
• pp.63-69
• /
• 2009

Gas volumetric fractions and pressure loss are very important parameters in understanding and predicting gas-liquid two-phase flows. They are also essential to design large heat exchanging system in many industries, boiler and refrigerating systems mounted at ships. This paper therefore presents a theoretical method of predicting the pressure loss and gas volumetric fractions in gas-liquid two-phase flows for the whole range of pipe inclinations. The theoretical analysis is based on the two-fluid stratified flow model. It also provides the results of the comparisons between this theoretical analysis results and previous experimental results.

• Korean Journal of Food Science and Technology
• /
• v.36 no.3
• /
• pp.518-521
• /
• 2004

Ginseng saponin was isolated from panax ginseng root using high-speed countercurrent chromatography (HSCCC). Preliminary studies were performed to optimize physical properties of two-phase solvent system and operating parameters including rotation speed of column, elution mode of mobile phase, and flow rate. Two-phase solvent system for isolation of ginseng saponins was composed of chloroform, water, and methanol as blending solvent. Chloroform-aqueous methanol (4:6) systems with various concentration of methanol in water were evaluated for retention of stationary phase in column. Retention of stationary phase decreased with increasing flow rate in tail-to-head elution mode using upper phase as mobile phase and head-to-tail elution mode using lower phase as mobile phase. Latter mode produced high retention at flow rate of 5 mL/min. Optimum conditions for isolation of saponin were chloroform/methanol/water (40/39/21) solvent system; mobile phase, of lower organic layer, flow rate, of 5 mL/min, head to tail elution mode, rotation speed, of 800 rpm, and sample injection, of $200{\mu}L$, Recovery yield of ginseng saponin from panax ginseng root extract by HSCCC was 63.6%, and the purity of HSCCC fractions was verified by TLC.

• The Transactions of the Korean Institute of Electrical Engineers A
• /
• v.50 no.5
• /
• pp.248-254
• /
• 2001

The traction power demand highly varies with time and train positions and the traction load is a large-capacity current at single phase converted from 3-phase power system. Subsequently, each phase current converted from 3-phase power system cannot be maintained in balance any longer and thus the traction load can bring about imbalance in three-phase voltage. Therefore, the exact assessment of voltage unbalance must be carried out preferentially as well as load forecast at stages of designing and planning for electric railway system. The evaluation of unbalance voltage in areas, such as electric railway depots should be a prerequisite with more accuracy. The conventional researches on voltage unbalance have dealt with connection schemes of the transformers used in ac AT-fed electric railroads system and induced formulas to briefly evaluate voltage unbalance in the system(3). These formulas are still being used widely due to their easy applicabilities on voltage unbalance evaluation. Meanwhile, they don't take into account detailed characteristics of ac AT-fed electric railroads system, being founded on some assumptions. Accordingly. accuracy still remains in question. This paper proposes a new method to more effectively estimate voltage unbalance index. In this method, numerous diverted circuits in electric railway depots are categorized in three components and each component is defined as a two-port network model. The equivalent circuit for the entire power supply system is also described into a two-port network model by making parallel and/or series connections of these components. Efficiency and accuracy in voltage unbalance calculation as well can be promoted by simplifying the circuits into two-port network models.

• Journal of Korean Institute of Industrial Engineers
• /
• v.20 no.3
• /
• pp.65-77
• /
• 1994

There are so many situations in which production schedules need to be rescheduled, due to the uncertainty or dynamic characteristics existing in manufacturing environment. Under the circumstances, it is necessary to build a system which helps a schedule manager understand the dynamic situations and reflect his insight into the system easily. We developed a system that can improve a production schedule. The system consists of a schedule editor and a schedule generator. The schedule editor, a rule-based system, always keeps a schedule feasible by keeping track of local changes in the revised one. The schedule generator consists of a two-phase heuristic method. In the lst phase it generates a schedule by a priority rule with Giffler and Thompson algorithm then it improves the performance in terms of the makespan in the 2nd phase. Experimental results show that, most of the times, the system generates a better schedule than the one with ordinary priority rules only.